GREMLIN Database
DUF466 - Protein of unknown function (DUF466)
PFAM: PF04328 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 62 (59)
Sequences: 6029 (3573)
Seq/√Len: 465.2
META: 0.868

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
40_S43_E3.5761.00
27_A31_A2.0631.00
47_E51_A2.0531.00
48_R51_A2.0321.00
29_M39_L1.9201.00
42_R46_R1.8691.00
50_E54_S1.8571.00
46_R50_E1.7681.00
15_L24_R1.7571.00
26_L38_P1.7551.00
23_E41_R1.7031.00
15_L20_P1.6081.00
29_M36_E1.5661.00
29_M38_P1.5361.00
26_L30_R1.4701.00
10_W14_R1.4011.00
25_Y39_L1.3601.00
12_Y15_L1.3071.00
41_R45_F1.3031.00
45_F49_Q1.2881.00
39_L47_E1.2321.00
27_A30_R1.2231.00
28_H33_H1.1981.00
21_D44_F1.1961.00
29_M37_P1.1341.00
5_A9_L1.0931.00
6_L9_L1.0771.00
47_E50_E1.0741.00
49_Q53_Y1.0711.00
14_R23_E1.0421.00
28_H32_H1.0331.00
9_L12_Y1.0211.00
10_W19_D1.0151.00
4_R7_R1.0101.00
20_P24_R1.0041.00
29_M34_P1.0001.00
6_L10_W0.9981.00
24_R27_A0.9451.00
11_R15_L0.9281.00
10_W13_L0.8961.00
12_Y16_V0.8871.00
4_R8_R0.8871.00
55_G58_G0.8811.00
42_R45_F0.8481.00
44_F48_R0.8431.00
33_H38_P0.8421.00
8_R11_R0.8421.00
32_H36_E0.8191.00
16_V19_D0.8161.00
8_R12_Y0.8071.00
26_L39_L0.8041.00
51_A54_S0.7961.00
49_Q52_R0.7921.00
45_F53_Y0.7241.00
3_R7_R0.7151.00
12_Y17_V0.6981.00
19_D45_F0.6901.00
10_W23_E0.6781.00
3_R6_L0.6691.00
23_E27_A0.6320.99
7_R11_R0.6320.99
14_R19_D0.6290.99
7_R10_W0.6260.99
28_H31_A0.6250.99
19_D44_F0.6190.99
14_R17_V0.6180.99
5_A8_R0.6140.99
33_H36_E0.6130.99
17_V20_P0.6080.99
11_R16_V0.6040.99
56_P60_R0.6010.99
55_G60_R0.5660.99
26_L29_M0.5640.99
46_R49_Q0.5620.99
3_R8_R0.5550.99
37_P43_E0.5510.99
44_F47_E0.5480.99
53_Y56_P0.5470.99
14_R20_P0.5440.98
14_R21_D0.5420.98
43_E46_R0.5400.98
50_E55_G0.5380.98
30_R35_G0.5370.98
9_L13_L0.5350.98
54_S57_G0.5230.98
45_F48_R0.5060.98
17_V41_R0.5010.97
20_P23_E0.5000.97
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3hkmA 1 0.3226 23.5 0.853 Contact Map
4v0qA 2 0.4194 13 0.869 Contact Map
4hdhA 2 0.4516 12.2 0.87 Contact Map
4magA 1 0.5806 10.6 0.874 Contact Map
4hhjA 1 0.4194 9.3 0.877 Contact Map
2bbrA 1 0.4194 7.4 0.883 Contact Map
2iw0A 1 0.5323 6.8 0.885 Contact Map
3tg2A 2 0.2581 6.7 0.885 Contact Map
4k6mA 2 0.5323 6.4 0.886 Contact Map
1t98A 2 0.371 6.2 0.887 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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