GREMLIN Database
DUF456 - Protein of unknown function (DUF456)
PFAM: PF04306 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 139 (136)
Sequences: 13080 (9002)
Seq/√Len: 771.9
META: 0.885

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
29_W33_V3.8061.00
12_F36_A3.1211.00
92_F110_G3.0341.00
62_R97_L2.8721.00
102_D105_Q2.6561.00
92_F103_L2.5321.00
11_I128_A2.2981.00
62_R98_R2.2811.00
33_V37_V2.1741.00
35_L131_M2.1221.00
54_A59_A2.0901.00
30_W33_V2.0531.00
8_P128_A2.0031.00
30_W34_V2.0001.00
61_K65_W1.9961.00
19_A24_F1.9831.00
31_T134_L1.8381.00
12_F39_A1.8111.00
38_L127_L1.7771.00
66_G94_G1.7751.00
46_D124_K1.7511.00
65_W69_I1.6701.00
99_G105_Q1.6661.00
15_L35_L1.6151.00
31_T35_L1.6121.00
111_L115_L1.5911.00
60_S63_G1.5331.00
42_G127_L1.5111.00
67_A91_A1.4901.00
76_F79_P1.4761.00
83_L87_P1.4651.00
66_G90_G1.4431.00
127_L131_M1.4071.00
15_L135_F1.4051.00
107_L111_L1.3941.00
16_L24_F1.3851.00
66_G70_G1.3731.00
13_A17_L1.3671.00
71_G87_P1.3421.00
90_G94_G1.3271.00
86_G90_G1.3001.00
44_V48_L1.2901.00
11_I132_I1.2671.00
57_F112_G1.2471.00
70_G90_G1.2341.00
96_L103_L1.1821.00
104_R107_L1.1811.00
22_D26_R1.1781.00
95_E98_R1.1591.00
12_F32_L1.1401.00
117_F121_T1.1341.00
94_G98_R1.1261.00
108_R111_L1.1191.00
12_F16_L1.1181.00
51_A64_I1.1121.00
133_G137_V1.1101.00
18_Y22_D1.0911.00
19_A27_V1.0851.00
11_I15_L1.0711.00
28_G31_T1.0701.00
8_P42_G1.0621.00
129_L133_G1.0601.00
12_F35_L1.0551.00
39_A131_M1.0361.00
62_R65_W1.0151.00
35_L39_A1.0061.00
118_L122_L1.0041.00
34_V134_L0.9941.00
78_G85_L0.9901.00
91_A110_G0.9891.00
103_L107_L0.9841.00
57_F109_A0.9701.00
40_L44_V0.9591.00
100_R105_Q0.9591.00
112_G116_G0.9561.00
93_L97_L0.9551.00
19_A32_L0.9531.00
49_A123_A0.9501.00
19_A29_W0.9481.00
37_V41_L0.9451.00
64_I68_A0.9351.00
130_V134_L0.9271.00
88_F92_F0.9191.00
119_L122_L0.9141.00
60_S98_R0.9121.00
104_R108_R0.9111.00
22_D25_A0.9081.00
105_Q108_R0.9071.00
43_E46_D0.9041.00
128_A132_I0.8761.00
9_P43_E0.8731.00
57_F111_L0.8701.00
96_L99_G0.8641.00
51_A68_A0.8631.00
132_I136_V0.8621.00
74_G86_G0.8571.00
45_L123_A0.8421.00
109_A112_G0.8401.00
53_G112_G0.8331.00
119_L126_A0.8331.00
78_G82_G0.8291.00
63_G95_E0.8291.00
8_P11_I0.8201.00
92_F96_L0.8091.00
11_I131_M0.8091.00
33_V36_A0.8081.00
92_F107_L0.7971.00
55_K61_K0.7971.00
99_G102_D0.7971.00
45_L49_A0.7931.00
16_L32_L0.7831.00
23_G26_R0.7801.00
18_Y21_A0.7751.00
54_A113_A0.7671.00
19_A23_G0.7671.00
75_L79_P0.7591.00
49_A53_G0.7571.00
85_L89_L0.7551.00
116_G120_G0.7511.00
79_P82_G0.7501.00
16_L20_W0.7441.00
103_L106_A0.7441.00
74_G79_P0.7411.00
8_P43_E0.7381.00
118_L121_T0.7341.00
82_G86_G0.7321.00
89_L93_L0.7251.00
59_A112_G0.7241.00
68_A71_G0.7101.00
48_L52_L0.7071.00
44_V47_Y0.7061.00
8_P12_F0.6971.00
27_V31_T0.6961.00
1_V4_V0.6901.00
57_F108_R0.6891.00
96_L102_D0.6881.00
115_L119_L0.6841.00
16_L36_A0.6801.00
63_G94_G0.6741.00
81_V85_L0.6721.00
63_G66_G0.6701.00
70_G74_G0.6691.00
62_R94_G0.6631.00
10_L14_G0.6621.00
9_P13_A0.6611.00
36_A39_A0.6571.00
75_L83_L0.6561.00
95_E109_A0.6501.00
32_L36_A0.6491.00
59_A113_A0.6481.00
53_G119_L0.6481.00
39_A127_L0.6451.00
78_G81_V0.6441.00
51_A54_A0.6431.00
88_F114_L0.6421.00
26_R30_W0.6371.00
19_A22_D0.6301.00
60_S95_E0.6271.00
67_A87_P0.6261.00
59_A109_A0.6231.00
121_T125_L0.6211.00
35_L134_L0.6111.00
38_L134_L0.6061.00
40_L43_E0.6051.00
65_W68_A0.6051.00
62_R66_G0.6051.00
70_G86_G0.5981.00
11_I18_Y0.5971.00
15_L131_M0.5961.00
20_W24_F0.5891.00
47_Y51_A0.5831.00
66_G91_A0.5801.00
54_A116_G0.5781.00
77_F86_G0.5781.00
126_A130_V0.5741.00
49_A52_L0.5731.00
74_G78_G0.5621.00
113_A117_F0.5601.00
123_A130_V0.5581.00
9_P39_A0.5571.00
57_F115_L0.5561.00
59_A116_G0.5551.00
52_L56_K0.5541.00
73_V77_F0.5521.00
87_P113_A0.5511.00
27_V135_F0.5491.00
50_P54_A0.5461.00
64_I67_A0.5441.00
35_L127_L0.5411.00
35_L38_L0.5371.00
105_Q109_A0.5321.00
49_A120_G0.5311.00
84_I117_F0.5291.00
11_I14_G0.5221.00
122_L126_A0.5181.00
1_V125_L0.5181.00
88_F110_G0.5141.00
111_L114_L0.5141.00
39_A43_E0.5131.00
66_G69_I0.5111.00
120_G124_K0.5111.00
14_G17_L0.5111.00
17_L20_W0.5011.00
4_V9_P0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2jwaA 2 0.295 4.9 0.924 Contact Map
2nwlA 3 0.7338 3.2 0.93 Contact Map
3qnqA 2 0.9712 3 0.931 Contact Map
2l2tA 2 0.3094 2.2 0.936 Contact Map
2ks1B 1 0.3094 2 0.937 Contact Map
4pj0K 1 0.1655 1.8 0.939 Contact Map
3wu2K 1 0.1655 1.7 0.94 Contact Map
2jlnA 1 1 1.7 0.94 Contact Map
2a65A 2 0.6115 1.6 0.941 Contact Map
1iijA 1 0.2518 1.6 0.941 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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