GREMLIN Database
DUF454 - Protein of unknown function (DUF454)
PFAM: PF04304 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 69 (68)
Sequences: 14108 (10527)
Seq/√Len: 1276.6
META: 0.865

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
3_R7_N2.7671.00
2_H6_L2.6711.00
16_R20_E2.1931.00
33_I68_L2.1731.00
25_P28_A2.0811.00
37_W64_A1.9291.00
40_I60_A1.8671.00
59_I63_V1.8541.00
6_L19_R1.8381.00
26_R30_I1.7171.00
4_W8_H1.6471.00
2_H19_R1.5641.00
31_L35_L1.5541.00
42_I46_F1.5311.00
51_W55_L1.4921.00
14_Y17_D1.4441.00
50_L53_R1.4401.00
8_H11_F1.4231.00
27_R31_L1.4071.00
36_M64_A1.2851.00
48_V53_R1.2721.00
50_L54_I1.2271.00
63_V67_I1.2191.00
5_L15_I1.2051.00
62_G65_I1.2021.00
43_S47_V1.1371.00
30_I34_A1.1331.00
32_A67_I1.1231.00
39_S43_S1.1211.00
28_A31_L1.0921.00
33_I64_A1.0791.00
58_A62_G1.0781.00
41_G45_F1.0771.00
24_I28_A1.0611.00
37_W60_A1.0551.00
17_D21_G1.0031.00
30_I33_I0.9971.00
62_G66_Y0.9911.00
35_L39_S0.9911.00
44_I53_R0.9821.00
52_L55_L0.9771.00
34_A38_L0.9721.00
54_I58_A0.9631.00
24_I32_A0.9501.00
13_P17_D0.9491.00
43_S46_F0.9431.00
63_V66_Y0.9291.00
44_I57_A0.9201.00
53_R57_A0.9191.00
64_A68_L0.8981.00
40_I57_A0.8841.00
42_I47_V0.8721.00
49_P53_R0.8691.00
43_S56_L0.8651.00
28_A32_A0.8351.00
38_L42_I0.8321.00
56_L60_A0.8311.00
12_G16_R0.8231.00
55_L59_I0.8161.00
6_L15_I0.8071.00
6_L16_R0.7741.00
33_I37_W0.7741.00
51_W54_I0.7701.00
17_D22_R0.7471.00
10_L13_P0.7421.00
61_L65_I0.7351.00
11_F14_Y0.7331.00
3_R6_L0.7291.00
17_D25_P0.7161.00
30_I68_L0.7161.00
8_H12_G0.7131.00
42_I45_F0.7041.00
36_M63_V0.7001.00
52_L56_L0.6981.00
60_A64_A0.6861.00
54_I57_A0.6681.00
37_W41_G0.6521.00
1_L4_W0.6411.00
16_R19_R0.6251.00
55_L58_A0.6181.00
49_P52_L0.6171.00
17_D23_G0.6071.00
45_F48_V0.6011.00
59_I66_Y0.5951.00
5_L11_F0.5811.00
27_R30_I0.5771.00
18_W37_W0.5731.00
7_N16_R0.5691.00
40_I44_I0.5641.00
41_G44_I0.5611.00
24_I67_I0.5571.00
53_R56_L0.5521.00
40_I53_R0.5391.00
14_Y25_P0.5331.00
5_L8_H0.5211.00
36_M60_A0.5141.00
37_W40_I0.5111.00
60_A63_V0.5071.00
40_I56_L0.5031.00
12_G15_I0.5011.00
57_A61_L0.5011.00
38_L41_G0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2ckaA 1 0.3768 11.7 0.832 Contact Map
4ekfA 1 0.4348 4.8 0.86 Contact Map
4huqT 1 0.7826 3.1 0.871 Contact Map
2ebzA 1 0.5072 3.1 0.872 Contact Map
4lxjA 1 0.5942 3.1 0.872 Contact Map
2ckcA 1 0.4058 2.9 0.874 Contact Map
3agtA 1 0.3623 2.7 0.876 Contact Map
1m0uA 2 0.3333 2.6 0.877 Contact Map
1yzvA 6 0.3768 2.5 0.878 Contact Map
1aw9A 5 0.3478 2.4 0.879 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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