DUF445 - PFAM28P - GREMLIN Contacts

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DUF445 - Protein of unknown function (DUF445)

PFAM:	PF04286	Sequences: AEAAMVGGLADWFAVTALFRHPLGLPIPFHTAIIPRNKDRIAESLGEFVEENFLTPEVVAERLRSADFAERLGRWLADPENAERLAREAARLLAGALEALDDEDVQRFLERALRRRLRELDLAPLLGRLLEALTADGRHQELLDLLLDRLARWLEDNETRARIRELIEERLPWWLPRFVDERLADRIVDGLLRLLDEVRADPDHPLRRAFDEALAELAERLQTDPELRARVEALKRELLAHPAVREWLESLWEELRAALLADLADPDSALRRRLAEALAALGERLAEDPALRAKLNRWLERAAVGLVERYRDEIGRLISETVERWDAEELSRRIELIVGRDLQFIRINGTLVGGLVGLLIYAVSQLI	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_10
Length:	367 (364)
Sequences:	5677 (4008)
Seq/√Len:	210.1
META:	0.692

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
37_N	40_R	5.285	1.00
67_D	70_E	4.298	1.00
21_H	25_L	4.263	1.00
219_E	223_T	3.935	1.00
232_E	235_K	3.763	1.00
67_D	296_N	3.298	1.00
131_E	222_Q	3.165	1.00
118_R	236_R	3.160	1.00
43_E	319_S	3.035	1.00
319_S	323_E	2.998	1.00
327_A	331_S	2.953	1.00
69_A	296_N	2.925	1.00
39_D	323_E	2.879	1.00
32_A	35_P	2.856	1.00
216_E	220_R	2.839	1.00
64_R	300_E	2.734	1.00
21_H	26_P	2.612	1.00
83_E	275_A	2.523	1.00
283_E	287_E	2.379	1.00
316_R	320_E	2.356	1.00
220_R	224_D	2.301	1.00
67_D	292_R	2.205	1.00
138_R	141_E	2.191	1.00
70_E	74_R	2.182	1.00
229_A	233_A	2.175	1.00
42_A	322_V	2.131	1.00
289_P	293_A	2.113	1.00
5_M	8_G	2.070	1.00
49_V	318_I	2.064	1.00
56_P	308_E	2.058	1.00
139_H	211_D	2.045	1.00
361_Y	365_Q	2.038	1.00
358_L	362_A	2.036	1.00
4_A	354_G	2.021	1.00
28_P	31_T	1.970	1.00
78_D	81_N	1.943	1.00
47_E	51_E	1.933	1.00
332_R	336_L	1.929	1.00
148_D	199_R	1.923	1.00
341_D	346_R	1.916	1.00
9_L	356_V	1.903	1.00
62_R	65_S	1.902	1.00
43_E	323_E	1.887	1.00
256_R	260_L	1.879	1.00
77_A	286_A	1.853	1.00
257_A	261_A	1.843	1.00
149_R	152_R	1.835	1.00
151_A	196_D	1.829	1.00
127_G	222_Q	1.817	1.00
313_E	316_R	1.807	1.00
356_V	360_I	1.804	1.00
21_H	32_A	1.790	1.00
87_R	271_R	1.785	1.00
56_P	60_A	1.783	1.00
341_D	348_N	1.775	1.00
328_E	332_R	1.757	1.00
209_A	212_E	1.751	1.00
326_D	329_E	1.749	1.00
121_D	228_R	1.733	1.00
21_H	36_R	1.719	1.00
338_V	342_L	1.713	1.00
165_E	169_E	1.712	1.00
102_D	256_R	1.705	1.00
116_R	119_E	1.699	1.00
38_K	42_A	1.699	1.00
152_R	156_D	1.696	1.00
34_I	41_I	1.635	1.00
82_A	279_A	1.608	1.00
344_F	347_I	1.606	1.00
73_G	282_G	1.600	1.00
334_I	338_V	1.595	1.00
151_A	199_R	1.587	1.00
79_P	83_E	1.575	1.00
130_L	218_A	1.570	1.00
144_D	207_R	1.563	1.00
143_L	210_F	1.555	1.00
121_D	232_E	1.529	1.00
262_D	265_D	1.524	1.00
330_L	334_I	1.521	1.00
103_E	107_R	1.516	1.00
50_E	315_G	1.515	1.00
80_E	83_E	1.508	1.00
83_E	87_R	1.506	1.00
135_A	215_A	1.500	1.00
16_T	20_R	1.490	1.00
359_L	363_V	1.486	1.00
140_Q	211_D	1.478	1.00
8_G	350_T	1.473	1.00
182_R	186_R	1.465	1.00
316_R	319_S	1.461	1.00
289_P	292_R	1.458	1.00
216_E	219_E	1.453	1.00
11_D	17_A	1.445	1.00
237_E	241_H	1.430	1.00
228_R	231_V	1.423	1.00
8_G	16_T	1.406	1.00
62_R	320_E	1.401	1.00
13_F	17_A	1.378	1.00
70_E	292_R	1.374	1.00
128_R	222_Q	1.359	1.00
171_L	180_D	1.356	1.00
46_G	50_E	1.336	1.00
11_D	15_V	1.333	1.00
10_A	13_F	1.308	1.00
351_L	354_G	1.307	1.00
147_L	195_L	1.272	1.00
196_D	200_A	1.260	1.00
8_G	11_D	1.249	1.00
10_A	15_V	1.244	1.00
284_R	288_D	1.237	1.00
10_A	17_A	1.236	1.00
82_A	282_G	1.228	1.00
327_A	330_L	1.224	1.00
203_D	208_R	1.220	1.00
38_K	327_A	1.218	1.00
115_R	119_E	1.214	1.00
233_A	237_E	1.210	1.00
3_A	10_A	1.180	1.00
50_E	312_D	1.172	1.00
52_N	333_R	1.169	1.00
38_K	48_F	1.162	1.00
258_A	261_A	1.157	1.00
335_E	339_G	1.153	1.00
151_A	155_E	1.151	1.00
349_G	353_G	1.149	1.00
118_R	240_A	1.143	1.00
42_A	46_G	1.134	1.00
94_A	264_A	1.130	1.00
41_I	50_E	1.129	1.00
91_R	95_G	1.128	1.00
35_P	41_I	1.128	1.00
101_D	104_D	1.127	1.00
157_N	160_R	1.122	1.00
86_A	275_A	1.111	1.00
148_D	200_A	1.106	1.00
220_R	227_L	1.102	1.00
353_G	357_G	1.099	1.00
343_Q	348_N	1.098	1.00
145_L	149_R	1.094	1.00
107_R	111_R	1.093	1.00
341_D	344_F	1.093	1.00
229_A	236_R	1.091	1.00
3_A	11_D	1.088	1.00
262_D	270_L	1.086	1.00
356_V	359_L	1.085	1.00
339_G	343_Q	1.085	1.00
362_A	365_Q	1.078	1.00
121_D	124_P	1.076	1.00
333_R	337_I	1.075	1.00
6_V	13_F	1.074	1.00
193_R	197_E	1.071	1.00
334_I	344_F	1.066	1.00
315_G	319_S	1.057	1.00
360_I	363_V	1.057	1.00
98_E	260_L	1.044	1.00
354_G	361_Y	1.044	1.00
9_L	13_F	1.043	1.00
294_K	298_W	1.042	1.00
208_R	212_E	1.041	1.00
17_A	31_T	1.037	1.00
260_L	264_A	1.037	1.00
42_A	45_L	1.036	1.00
236_R	240_A	1.035	1.00
143_L	147_L	1.033	1.00
14_A	19_F	1.032	1.00
291_L	295_L	1.031	1.00
343_Q	350_T	1.029	1.00
57_E	61_E	1.028	1.00
55_T	58_V	1.025	1.00
329_E	333_R	1.023	1.00
74_R	286_A	1.022	1.00
346_R	350_T	1.022	1.00
182_R	185_D	1.022	1.00
343_Q	346_R	1.019	1.00
82_A	275_A	1.018	1.00
197_E	201_D	1.013	1.00
19_F	22_P	1.010	1.00
176_P	179_V	1.003	1.00
43_E	47_E	1.001	1.00
298_W	302_A	1.000	1.00
194_L	206_L	0.992	1.00
243_A	246_E	0.987	1.00
230_R	233_A	0.986	1.00
175_L	180_D	0.985	1.00
8_G	13_F	0.985	1.00
60_A	304_V	0.984	1.00
137_G	140_Q	0.983	1.00
16_T	31_T	0.980	1.00
11_D	31_T	0.980	1.00
273_R	276_E	0.979	1.00
7_G	14_A	0.979	1.00
89_A	93_L	0.975	1.00
2_E	30_H	0.973	1.00
320_E	323_E	0.973	1.00
46_G	319_S	0.972	1.00
346_R	352_V	0.970	1.00
184_A	187_I	0.967	1.00
212_E	216_E	0.962	1.00
26_P	30_H	0.949	0.99
279_A	283_E	0.949	0.99
335_E	346_R	0.943	0.99
344_F	348_N	0.940	0.99
104_D	107_R	0.940	0.99
37_N	41_I	0.939	0.99
3_A	8_G	0.939	0.99
335_E	343_Q	0.938	0.99
225_P	229_A	0.933	0.99
116_R	120_L	0.931	0.99
321_T	324_R	0.930	0.99
255_L	258_A	0.926	0.99
149_R	153_W	0.926	0.99
283_E	286_A	0.925	0.99
156_D	159_T	0.924	0.99
12_W	16_T	0.920	0.99
166_L	169_E	0.919	0.99
262_D	268_S	0.915	0.99
39_D	43_E	0.914	0.99
155_E	192_L	0.911	0.99
13_F	31_T	0.911	0.99
2_E	13_F	0.909	0.99
160_R	164_R	0.908	0.99
228_R	232_E	0.900	0.99
226_E	230_R	0.900	0.99
71_R	74_R	0.895	0.99
171_L	175_L	0.888	0.99
151_A	192_L	0.885	0.99
131_E	219_E	0.884	0.99
58_V	62_R	0.881	0.99
39_D	42_A	0.878	0.99
235_K	239_L	0.872	0.99
17_A	30_H	0.870	0.99
233_A	236_R	0.869	0.99
325_W	344_F	0.867	0.99
75_W	81_N	0.867	0.99
108_F	112_A	0.864	0.99
329_E	332_R	0.862	0.99
53_F	325_W	0.860	0.99
231_V	234_L	0.858	0.99
11_D	16_T	0.858	0.99
77_A	283_E	0.856	0.99
3_A	6_V	0.856	0.99
341_D	352_V	0.855	0.99
272_R	276_E	0.853	0.99
17_A	32_A	0.847	0.99
325_W	335_E	0.847	0.99
2_E	6_V	0.846	0.99
61_E	324_R	0.844	0.99
290_A	293_A	0.842	0.99
335_E	350_T	0.841	0.99
6_V	16_T	0.841	0.99
11_D	30_H	0.839	0.99
27_I	30_H	0.839	0.99
45_L	322_V	0.837	0.99
65_S	68_F	0.834	0.99
60_A	64_R	0.832	0.99
275_A	279_A	0.831	0.99
95_G	98_E	0.831	0.99
170_R	174_W	0.829	0.99
16_T	30_H	0.826	0.99
23_L	27_I	0.825	0.99
226_E	229_A	0.825	0.99
276_E	280_A	0.824	0.99
171_L	179_V	0.821	0.99
331_S	335_E	0.821	0.99
250_S	254_E	0.818	0.98
297_R	301_R	0.818	0.98
348_N	352_V	0.816	0.98
268_S	271_R	0.812	0.98
41_I	334_I	0.808	0.98
12_W	48_F	0.805	0.98
305_G	309_R	0.803	0.98
124_P	128_R	0.793	0.98
10_A	31_T	0.792	0.98
347_I	351_L	0.792	0.98
131_E	215_A	0.791	0.98
209_A	213_A	0.788	0.98
6_V	31_T	0.780	0.98
21_H	27_I	0.778	0.98
307_V	310_Y	0.777	0.98
44_S	337_I	0.775	0.98
16_T	32_A	0.774	0.98
67_D	74_R	0.769	0.98
30_H	33_I	0.766	0.98
104_D	294_K	0.763	0.98
293_A	297_R	0.762	0.98
325_W	333_R	0.761	0.98
127_G	218_A	0.760	0.98
80_E	84_R	0.758	0.98
343_Q	360_I	0.757	0.98
350_T	361_Y	0.757	0.98
139_H	144_D	0.754	0.97
227_L	231_V	0.750	0.97
3_A	7_G	0.750	0.97
261_A	265_D	0.747	0.97
188_V	192_L	0.746	0.97
54_L	62_R	0.746	0.97
131_E	135_A	0.745	0.97
341_D	347_I	0.744	0.97
89_A	92_L	0.743	0.97
82_A	86_A	0.741	0.97
110_E	114_R	0.737	0.97
163_I	167_I	0.737	0.97
96_A	100_L	0.733	0.97
91_R	309_R	0.733	0.97
201_D	204_H	0.731	0.97
91_R	94_A	0.731	0.97
338_V	346_R	0.729	0.97
304_V	308_E	0.728	0.97
10_A	16_T	0.726	0.97
186_R	190_G	0.723	0.97
169_E	173_W	0.722	0.97
35_P	328_E	0.721	0.97
46_G	322_V	0.720	0.97
217_L	227_L	0.717	0.97
114_R	118_R	0.717	0.97
349_G	357_G	0.717	0.97
48_F	333_R	0.716	0.97
296_N	300_E	0.715	0.96
73_G	77_A	0.714	0.96
202_P	205_P	0.713	0.96
225_P	228_R	0.713	0.96
12_W	20_R	0.713	0.96
282_G	285_L	0.710	0.96
330_L	337_I	0.709	0.96
3_A	13_F	0.708	0.96
15_V	44_S	0.708	0.96
12_W	346_R	0.708	0.96
4_A	7_G	0.705	0.96
27_I	31_T	0.703	0.96
12_W	17_A	0.701	0.96
302_A	306_L	0.698	0.96
262_D	269_A	0.698	0.96
39_D	327_A	0.696	0.96
24_G	27_I	0.696	0.96
42_A	323_E	0.695	0.96
258_A	262_D	0.694	0.96
63_L	307_V	0.693	0.96
46_G	49_V	0.693	0.96
123_A	231_V	0.692	0.96
320_E	324_R	0.692	0.96
134_T	215_A	0.690	0.96
173_W	182_R	0.690	0.96
12_W	342_L	0.689	0.96
180_D	183_L	0.688	0.96
95_G	99_A	0.688	0.96
317_L	321_T	0.687	0.96
2_E	11_D	0.687	0.96
322_V	337_I	0.687	0.96
7_G	10_A	0.684	0.95
164_R	181_E	0.684	0.95
48_F	325_W	0.684	0.95
356_V	361_Y	0.684	0.95
291_L	294_K	0.683	0.95
41_I	58_V	0.683	0.95
335_E	341_D	0.674	0.95
40_R	48_F	0.673	0.95
6_V	30_H	0.673	0.95
44_S	47_E	0.672	0.95
181_E	185_D	0.672	0.95
146_L	149_R	0.669	0.95
325_W	346_R	0.669	0.95
242_P	247_W	0.668	0.95
290_A	294_K	0.667	0.95
232_E	236_R	0.667	0.95
352_V	356_V	0.667	0.95
26_P	31_T	0.666	0.95
166_L	170_R	0.664	0.95
31_T	346_R	0.664	0.95
175_L	178_F	0.663	0.95
224_D	227_L	0.663	0.95
3_A	12_W	0.662	0.95
246_E	249_E	0.660	0.95
134_T	139_H	0.659	0.94
217_L	220_R	0.658	0.94
192_L	196_D	0.656	0.94
151_A	195_L	0.654	0.94
8_G	31_T	0.653	0.94
48_F	52_N	0.652	0.94
7_G	19_F	0.649	0.94
11_D	32_A	0.649	0.94
175_L	179_V	0.649	0.94
267_D	272_R	0.648	0.94
4_A	9_L	0.648	0.94
81_N	84_R	0.647	0.94
81_N	85_L	0.645	0.94
360_I	364_S	0.644	0.94
313_E	330_L	0.643	0.94
12_W	40_R	0.642	0.94
278_L	281_L	0.641	0.94
327_A	337_I	0.641	0.94
110_E	245_R	0.641	0.94
312_D	315_G	0.640	0.94
13_F	30_H	0.639	0.94
331_S	338_V	0.638	0.93
79_P	85_L	0.637	0.93
10_A	30_H	0.637	0.93
201_D	205_P	0.637	0.93
292_R	296_N	0.636	0.93
167_I	171_L	0.635	0.93
99_A	105_V	0.634	0.93
355_L	360_I	0.633	0.93
197_E	204_H	0.632	0.93
12_W	31_T	0.630	0.93
286_A	292_R	0.630	0.93
333_R	360_I	0.630	0.93
315_G	321_T	0.630	0.93
2_E	10_A	0.624	0.93
42_A	48_F	0.623	0.93
270_L	273_R	0.621	0.93
12_W	15_V	0.621	0.93
311_R	319_S	0.621	0.93
8_G	354_G	0.619	0.92
50_E	58_V	0.619	0.92
331_S	337_I	0.619	0.92
8_G	17_A	0.618	0.92
321_T	325_W	0.616	0.92
74_R	77_A	0.615	0.92
112_A	115_R	0.614	0.92
84_R	87_R	0.610	0.92
247_W	251_L	0.609	0.92
37_N	52_N	0.608	0.92
344_F	361_Y	0.608	0.92
51_E	55_T	0.608	0.92
335_E	348_N	0.607	0.92
44_S	48_F	0.604	0.92
15_V	18_L	0.604	0.92
186_R	189_D	0.603	0.91
205_P	210_F	0.603	0.91
331_S	334_I	0.603	0.91
73_G	285_L	0.603	0.91
33_I	48_F	0.601	0.91
288_D	293_A	0.601	0.91
269_A	272_R	0.600	0.91
61_E	65_S	0.599	0.91
345_I	353_G	0.599	0.91
11_D	348_N	0.599	0.91
45_L	53_F	0.599	0.91
9_L	12_W	0.599	0.91
220_R	223_T	0.596	0.91
187_I	314_I	0.595	0.91
322_V	327_A	0.594	0.91
254_E	258_A	0.593	0.91
334_I	348_N	0.591	0.91
182_R	190_G	0.590	0.91
230_R	234_L	0.589	0.91
327_A	338_V	0.587	0.90
106_Q	110_E	0.583	0.90
61_E	64_R	0.582	0.90
210_F	214_L	0.581	0.90
35_P	42_A	0.581	0.90
322_V	325_W	0.580	0.90
185_D	188_V	0.579	0.90
101_D	113_L	0.575	0.89
340_R	344_F	0.573	0.89
346_R	360_I	0.571	0.89
99_A	294_K	0.570	0.89
93_L	96_A	0.570	0.89
326_D	341_D	0.570	0.89
126_L	130_L	0.569	0.89
347_I	364_S	0.568	0.89
48_F	53_F	0.565	0.89
8_G	30_H	0.565	0.89
83_E	240_A	0.564	0.89
180_D	197_E	0.564	0.89
359_L	362_A	0.563	0.88
163_I	191_L	0.563	0.88
198_V	206_L	0.563	0.88
284_R	295_L	0.562	0.88
116_R	135_A	0.562	0.88
171_L	176_P	0.561	0.88
155_E	158_E	0.561	0.88
115_R	118_R	0.561	0.88
11_D	346_R	0.561	0.88
13_F	16_T	0.560	0.88
358_L	361_Y	0.560	0.88
325_W	341_D	0.559	0.88
2_E	53_F	0.558	0.88
10_A	348_N	0.558	0.88
224_D	228_R	0.557	0.88
111_R	115_R	0.557	0.88
337_I	356_V	0.557	0.88
348_N	360_I	0.555	0.88
6_V	33_I	0.555	0.88
191_L	195_L	0.554	0.88
338_V	348_N	0.554	0.88
144_D	147_L	0.554	0.88
347_I	352_V	0.552	0.87
234_L	237_E	0.552	0.87
31_T	37_N	0.552	0.87
46_G	315_G	0.551	0.87
303_A	333_R	0.550	0.87
243_A	248_L	0.549	0.87
140_Q	207_R	0.547	0.87
321_T	333_R	0.544	0.87
321_T	331_S	0.543	0.87
244_V	248_L	0.542	0.87
24_G	30_H	0.541	0.86
22_P	35_P	0.541	0.86
345_I	349_G	0.540	0.86
11_D	14_A	0.539	0.86
69_A	72_L	0.539	0.86
174_W	177_R	0.537	0.86
205_P	209_A	0.536	0.86
171_L	174_W	0.535	0.86
10_A	14_A	0.534	0.86
339_G	360_I	0.534	0.86
97_L	260_L	0.534	0.86
9_L	16_T	0.534	0.86
7_G	11_D	0.533	0.86
35_P	38_K	0.532	0.86
338_V	341_D	0.532	0.86
20_R	335_E	0.532	0.86
193_R	196_D	0.532	0.86
341_D	350_T	0.530	0.85
85_L	278_L	0.529	0.85
18_L	41_I	0.526	0.85
298_W	306_L	0.524	0.85
102_D	108_F	0.524	0.85
273_R	277_A	0.524	0.85
242_P	246_E	0.523	0.85
335_E	342_L	0.523	0.85
211_D	215_A	0.523	0.85
192_L	199_R	0.522	0.84
33_I	338_V	0.516	0.84
3_A	50_E	0.516	0.84
216_E	228_R	0.516	0.84
301_R	305_G	0.515	0.84
342_L	346_R	0.515	0.84
105_V	109_L	0.513	0.83
344_F	350_T	0.512	0.83
318_I	350_T	0.512	0.83
318_I	345_I	0.511	0.83
57_E	60_A	0.510	0.83
68_F	71_R	0.509	0.83
123_A	127_G	0.509	0.83
334_I	341_D	0.508	0.83
44_S	338_V	0.507	0.83
341_D	354_G	0.507	0.83
90_A	271_R	0.506	0.83
215_A	219_E	0.505	0.82
153_W	156_D	0.505	0.82
106_Q	253_E	0.504	0.82
8_G	12_W	0.502	0.82
26_P	32_A	0.501	0.82
348_N	355_L	0.501	0.82
346_R	361_Y	0.500	0.82

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
2kluA	1	0.079	38.9	0.949	Contact Map
3onoA	4	0.2343	38.5	0.949	Contact Map
4u8fA	4	0.2425	19.2	0.956	Contact Map
2ks1B	1	0.0899	13.8	0.959	Contact Map
2j69A	1	0.376	12.2	0.96	Contact Map
2kncB	1	0.0899	11.5	0.96	Contact Map
4aybC	1	0.2044	11.3	0.96	Contact Map
2l2tA	2	0.0872	10.7	0.961	Contact Map
2m20A	2	0.0681	9.2	0.962	Contact Map
3c5yA	5	0.2316	9.2	0.962	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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