GREMLIN Database
DUF445 - Protein of unknown function (DUF445)
PFAM: PF04286 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 367 (364)
Sequences: 5677 (4008)
Seq/√Len: 210.1
META: 0.692

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
37_N40_R5.2851.00
67_D70_E4.2981.00
21_H25_L4.2631.00
219_E223_T3.9351.00
232_E235_K3.7631.00
67_D296_N3.2981.00
131_E222_Q3.1651.00
118_R236_R3.1601.00
43_E319_S3.0351.00
319_S323_E2.9981.00
327_A331_S2.9531.00
69_A296_N2.9251.00
39_D323_E2.8791.00
32_A35_P2.8561.00
216_E220_R2.8391.00
64_R300_E2.7341.00
21_H26_P2.6121.00
83_E275_A2.5231.00
283_E287_E2.3791.00
316_R320_E2.3561.00
220_R224_D2.3011.00
67_D292_R2.2051.00
138_R141_E2.1911.00
70_E74_R2.1821.00
229_A233_A2.1751.00
42_A322_V2.1311.00
289_P293_A2.1131.00
5_M8_G2.0701.00
49_V318_I2.0641.00
56_P308_E2.0581.00
139_H211_D2.0451.00
361_Y365_Q2.0381.00
358_L362_A2.0361.00
4_A354_G2.0211.00
28_P31_T1.9701.00
78_D81_N1.9431.00
47_E51_E1.9331.00
332_R336_L1.9291.00
148_D199_R1.9231.00
341_D346_R1.9161.00
9_L356_V1.9031.00
62_R65_S1.9021.00
43_E323_E1.8871.00
256_R260_L1.8791.00
77_A286_A1.8531.00
257_A261_A1.8431.00
149_R152_R1.8351.00
151_A196_D1.8291.00
127_G222_Q1.8171.00
313_E316_R1.8071.00
356_V360_I1.8041.00
21_H32_A1.7901.00
87_R271_R1.7851.00
56_P60_A1.7831.00
341_D348_N1.7751.00
328_E332_R1.7571.00
209_A212_E1.7511.00
326_D329_E1.7491.00
121_D228_R1.7331.00
21_H36_R1.7191.00
338_V342_L1.7131.00
165_E169_E1.7121.00
102_D256_R1.7051.00
116_R119_E1.6991.00
38_K42_A1.6991.00
152_R156_D1.6961.00
34_I41_I1.6351.00
82_A279_A1.6081.00
344_F347_I1.6061.00
73_G282_G1.6001.00
334_I338_V1.5951.00
151_A199_R1.5871.00
79_P83_E1.5751.00
130_L218_A1.5701.00
144_D207_R1.5631.00
143_L210_F1.5551.00
121_D232_E1.5291.00
262_D265_D1.5241.00
330_L334_I1.5211.00
103_E107_R1.5161.00
50_E315_G1.5151.00
80_E83_E1.5081.00
83_E87_R1.5061.00
135_A215_A1.5001.00
16_T20_R1.4901.00
359_L363_V1.4861.00
140_Q211_D1.4781.00
8_G350_T1.4731.00
182_R186_R1.4651.00
316_R319_S1.4611.00
289_P292_R1.4581.00
216_E219_E1.4531.00
11_D17_A1.4451.00
237_E241_H1.4301.00
228_R231_V1.4231.00
8_G16_T1.4061.00
62_R320_E1.4011.00
13_F17_A1.3781.00
70_E292_R1.3741.00
128_R222_Q1.3591.00
171_L180_D1.3561.00
46_G50_E1.3361.00
11_D15_V1.3331.00
10_A13_F1.3081.00
351_L354_G1.3071.00
147_L195_L1.2721.00
196_D200_A1.2601.00
8_G11_D1.2491.00
10_A15_V1.2441.00
284_R288_D1.2371.00
10_A17_A1.2361.00
82_A282_G1.2281.00
327_A330_L1.2241.00
203_D208_R1.2201.00
38_K327_A1.2181.00
115_R119_E1.2141.00
233_A237_E1.2101.00
3_A10_A1.1801.00
50_E312_D1.1721.00
52_N333_R1.1691.00
38_K48_F1.1621.00
258_A261_A1.1571.00
335_E339_G1.1531.00
151_A155_E1.1511.00
349_G353_G1.1491.00
118_R240_A1.1431.00
42_A46_G1.1341.00
94_A264_A1.1301.00
41_I50_E1.1291.00
91_R95_G1.1281.00
35_P41_I1.1281.00
101_D104_D1.1271.00
157_N160_R1.1221.00
86_A275_A1.1111.00
148_D200_A1.1061.00
220_R227_L1.1021.00
353_G357_G1.0991.00
343_Q348_N1.0981.00
145_L149_R1.0941.00
107_R111_R1.0931.00
341_D344_F1.0931.00
229_A236_R1.0911.00
3_A11_D1.0881.00
262_D270_L1.0861.00
356_V359_L1.0851.00
339_G343_Q1.0851.00
362_A365_Q1.0781.00
121_D124_P1.0761.00
333_R337_I1.0751.00
6_V13_F1.0741.00
193_R197_E1.0711.00
334_I344_F1.0661.00
315_G319_S1.0571.00
360_I363_V1.0571.00
98_E260_L1.0441.00
354_G361_Y1.0441.00
9_L13_F1.0431.00
294_K298_W1.0421.00
208_R212_E1.0411.00
17_A31_T1.0371.00
260_L264_A1.0371.00
42_A45_L1.0361.00
236_R240_A1.0351.00
143_L147_L1.0331.00
14_A19_F1.0321.00
291_L295_L1.0311.00
343_Q350_T1.0291.00
57_E61_E1.0281.00
55_T58_V1.0251.00
329_E333_R1.0231.00
74_R286_A1.0221.00
346_R350_T1.0221.00
182_R185_D1.0221.00
343_Q346_R1.0191.00
82_A275_A1.0181.00
197_E201_D1.0131.00
19_F22_P1.0101.00
176_P179_V1.0031.00
43_E47_E1.0011.00
298_W302_A1.0001.00
194_L206_L0.9921.00
243_A246_E0.9871.00
230_R233_A0.9861.00
175_L180_D0.9851.00
8_G13_F0.9851.00
60_A304_V0.9841.00
137_G140_Q0.9831.00
16_T31_T0.9801.00
11_D31_T0.9801.00
273_R276_E0.9791.00
7_G14_A0.9791.00
89_A93_L0.9751.00
2_E30_H0.9731.00
320_E323_E0.9731.00
46_G319_S0.9721.00
346_R352_V0.9701.00
184_A187_I0.9671.00
212_E216_E0.9621.00
26_P30_H0.9490.99
279_A283_E0.9490.99
335_E346_R0.9430.99
344_F348_N0.9400.99
104_D107_R0.9400.99
37_N41_I0.9390.99
3_A8_G0.9390.99
335_E343_Q0.9380.99
225_P229_A0.9330.99
116_R120_L0.9310.99
321_T324_R0.9300.99
255_L258_A0.9260.99
149_R153_W0.9260.99
283_E286_A0.9250.99
156_D159_T0.9240.99
12_W16_T0.9200.99
166_L169_E0.9190.99
262_D268_S0.9150.99
39_D43_E0.9140.99
155_E192_L0.9110.99
13_F31_T0.9110.99
2_E13_F0.9090.99
160_R164_R0.9080.99
228_R232_E0.9000.99
226_E230_R0.9000.99
71_R74_R0.8950.99
171_L175_L0.8880.99
151_A192_L0.8850.99
131_E219_E0.8840.99
58_V62_R0.8810.99
39_D42_A0.8780.99
235_K239_L0.8720.99
17_A30_H0.8700.99
233_A236_R0.8690.99
325_W344_F0.8670.99
75_W81_N0.8670.99
108_F112_A0.8640.99
329_E332_R0.8620.99
53_F325_W0.8600.99
231_V234_L0.8580.99
11_D16_T0.8580.99
77_A283_E0.8560.99
3_A6_V0.8560.99
341_D352_V0.8550.99
272_R276_E0.8530.99
17_A32_A0.8470.99
325_W335_E0.8470.99
2_E6_V0.8460.99
61_E324_R0.8440.99
290_A293_A0.8420.99
335_E350_T0.8410.99
6_V16_T0.8410.99
11_D30_H0.8390.99
27_I30_H0.8390.99
45_L322_V0.8370.99
65_S68_F0.8340.99
60_A64_R0.8320.99
275_A279_A0.8310.99
95_G98_E0.8310.99
170_R174_W0.8290.99
16_T30_H0.8260.99
23_L27_I0.8250.99
226_E229_A0.8250.99
276_E280_A0.8240.99
171_L179_V0.8210.99
331_S335_E0.8210.99
250_S254_E0.8180.98
297_R301_R0.8180.98
348_N352_V0.8160.98
268_S271_R0.8120.98
41_I334_I0.8080.98
12_W48_F0.8050.98
305_G309_R0.8030.98
124_P128_R0.7930.98
10_A31_T0.7920.98
347_I351_L0.7920.98
131_E215_A0.7910.98
209_A213_A0.7880.98
6_V31_T0.7800.98
21_H27_I0.7780.98
307_V310_Y0.7770.98
44_S337_I0.7750.98
16_T32_A0.7740.98
67_D74_R0.7690.98
30_H33_I0.7660.98
104_D294_K0.7630.98
293_A297_R0.7620.98
325_W333_R0.7610.98
127_G218_A0.7600.98
80_E84_R0.7580.98
343_Q360_I0.7570.98
350_T361_Y0.7570.98
139_H144_D0.7540.97
227_L231_V0.7500.97
3_A7_G0.7500.97
261_A265_D0.7470.97
188_V192_L0.7460.97
54_L62_R0.7460.97
131_E135_A0.7450.97
341_D347_I0.7440.97
89_A92_L0.7430.97
82_A86_A0.7410.97
110_E114_R0.7370.97
163_I167_I0.7370.97
96_A100_L0.7330.97
91_R309_R0.7330.97
201_D204_H0.7310.97
91_R94_A0.7310.97
338_V346_R0.7290.97
304_V308_E0.7280.97
10_A16_T0.7260.97
186_R190_G0.7230.97
169_E173_W0.7220.97
35_P328_E0.7210.97
46_G322_V0.7200.97
217_L227_L0.7170.97
114_R118_R0.7170.97
349_G357_G0.7170.97
48_F333_R0.7160.97
296_N300_E0.7150.96
73_G77_A0.7140.96
202_P205_P0.7130.96
225_P228_R0.7130.96
12_W20_R0.7130.96
282_G285_L0.7100.96
330_L337_I0.7090.96
3_A13_F0.7080.96
15_V44_S0.7080.96
12_W346_R0.7080.96
4_A7_G0.7050.96
27_I31_T0.7030.96
12_W17_A0.7010.96
302_A306_L0.6980.96
262_D269_A0.6980.96
39_D327_A0.6960.96
24_G27_I0.6960.96
42_A323_E0.6950.96
258_A262_D0.6940.96
63_L307_V0.6930.96
46_G49_V0.6930.96
123_A231_V0.6920.96
320_E324_R0.6920.96
134_T215_A0.6900.96
173_W182_R0.6900.96
12_W342_L0.6890.96
180_D183_L0.6880.96
95_G99_A0.6880.96
317_L321_T0.6870.96
2_E11_D0.6870.96
322_V337_I0.6870.96
7_G10_A0.6840.95
164_R181_E0.6840.95
48_F325_W0.6840.95
356_V361_Y0.6840.95
291_L294_K0.6830.95
41_I58_V0.6830.95
335_E341_D0.6740.95
40_R48_F0.6730.95
6_V30_H0.6730.95
44_S47_E0.6720.95
181_E185_D0.6720.95
146_L149_R0.6690.95
325_W346_R0.6690.95
242_P247_W0.6680.95
290_A294_K0.6670.95
232_E236_R0.6670.95
352_V356_V0.6670.95
26_P31_T0.6660.95
166_L170_R0.6640.95
31_T346_R0.6640.95
175_L178_F0.6630.95
224_D227_L0.6630.95
3_A12_W0.6620.95
246_E249_E0.6600.95
134_T139_H0.6590.94
217_L220_R0.6580.94
192_L196_D0.6560.94
151_A195_L0.6540.94
8_G31_T0.6530.94
48_F52_N0.6520.94
7_G19_F0.6490.94
11_D32_A0.6490.94
175_L179_V0.6490.94
267_D272_R0.6480.94
4_A9_L0.6480.94
81_N84_R0.6470.94
81_N85_L0.6450.94
360_I364_S0.6440.94
313_E330_L0.6430.94
12_W40_R0.6420.94
278_L281_L0.6410.94
327_A337_I0.6410.94
110_E245_R0.6410.94
312_D315_G0.6400.94
13_F30_H0.6390.94
331_S338_V0.6380.93
79_P85_L0.6370.93
10_A30_H0.6370.93
201_D205_P0.6370.93
292_R296_N0.6360.93
167_I171_L0.6350.93
99_A105_V0.6340.93
355_L360_I0.6330.93
197_E204_H0.6320.93
12_W31_T0.6300.93
286_A292_R0.6300.93
333_R360_I0.6300.93
315_G321_T0.6300.93
2_E10_A0.6240.93
42_A48_F0.6230.93
270_L273_R0.6210.93
12_W15_V0.6210.93
311_R319_S0.6210.93
8_G354_G0.6190.92
50_E58_V0.6190.92
331_S337_I0.6190.92
8_G17_A0.6180.92
321_T325_W0.6160.92
74_R77_A0.6150.92
112_A115_R0.6140.92
84_R87_R0.6100.92
247_W251_L0.6090.92
37_N52_N0.6080.92
344_F361_Y0.6080.92
51_E55_T0.6080.92
335_E348_N0.6070.92
44_S48_F0.6040.92
15_V18_L0.6040.92
186_R189_D0.6030.91
205_P210_F0.6030.91
331_S334_I0.6030.91
73_G285_L0.6030.91
33_I48_F0.6010.91
288_D293_A0.6010.91
269_A272_R0.6000.91
61_E65_S0.5990.91
345_I353_G0.5990.91
11_D348_N0.5990.91
45_L53_F0.5990.91
9_L12_W0.5990.91
220_R223_T0.5960.91
187_I314_I0.5950.91
322_V327_A0.5940.91
254_E258_A0.5930.91
334_I348_N0.5910.91
182_R190_G0.5900.91
230_R234_L0.5890.91
327_A338_V0.5870.90
106_Q110_E0.5830.90
61_E64_R0.5820.90
210_F214_L0.5810.90
35_P42_A0.5810.90
322_V325_W0.5800.90
185_D188_V0.5790.90
101_D113_L0.5750.89
340_R344_F0.5730.89
346_R360_I0.5710.89
99_A294_K0.5700.89
93_L96_A0.5700.89
326_D341_D0.5700.89
126_L130_L0.5690.89
347_I364_S0.5680.89
48_F53_F0.5650.89
8_G30_H0.5650.89
83_E240_A0.5640.89
180_D197_E0.5640.89
359_L362_A0.5630.88
163_I191_L0.5630.88
198_V206_L0.5630.88
284_R295_L0.5620.88
116_R135_A0.5620.88
171_L176_P0.5610.88
155_E158_E0.5610.88
115_R118_R0.5610.88
11_D346_R0.5610.88
13_F16_T0.5600.88
358_L361_Y0.5600.88
325_W341_D0.5590.88
2_E53_F0.5580.88
10_A348_N0.5580.88
224_D228_R0.5570.88
111_R115_R0.5570.88
337_I356_V0.5570.88
348_N360_I0.5550.88
6_V33_I0.5550.88
191_L195_L0.5540.88
338_V348_N0.5540.88
144_D147_L0.5540.88
347_I352_V0.5520.87
234_L237_E0.5520.87
31_T37_N0.5520.87
46_G315_G0.5510.87
303_A333_R0.5500.87
243_A248_L0.5490.87
140_Q207_R0.5470.87
321_T333_R0.5440.87
321_T331_S0.5430.87
244_V248_L0.5420.87
24_G30_H0.5410.86
22_P35_P0.5410.86
345_I349_G0.5400.86
11_D14_A0.5390.86
69_A72_L0.5390.86
174_W177_R0.5370.86
205_P209_A0.5360.86
171_L174_W0.5350.86
10_A14_A0.5340.86
339_G360_I0.5340.86
97_L260_L0.5340.86
9_L16_T0.5340.86
7_G11_D0.5330.86
35_P38_K0.5320.86
338_V341_D0.5320.86
20_R335_E0.5320.86
193_R196_D0.5320.86
341_D350_T0.5300.85
85_L278_L0.5290.85
18_L41_I0.5260.85
298_W306_L0.5240.85
102_D108_F0.5240.85
273_R277_A0.5240.85
242_P246_E0.5230.85
335_E342_L0.5230.85
211_D215_A0.5230.85
192_L199_R0.5220.84
33_I338_V0.5160.84
3_A50_E0.5160.84
216_E228_R0.5160.84
301_R305_G0.5150.84
342_L346_R0.5150.84
105_V109_L0.5130.83
344_F350_T0.5120.83
318_I350_T0.5120.83
318_I345_I0.5110.83
57_E60_A0.5100.83
68_F71_R0.5090.83
123_A127_G0.5090.83
334_I341_D0.5080.83
44_S338_V0.5070.83
341_D354_G0.5070.83
90_A271_R0.5060.83
215_A219_E0.5050.82
153_W156_D0.5050.82
106_Q253_E0.5040.82
8_G12_W0.5020.82
26_P32_A0.5010.82
348_N355_L0.5010.82
346_R361_Y0.5000.82
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2kluA 1 0.079 38.9 0.949 Contact Map
3onoA 4 0.2343 38.5 0.949 Contact Map
4u8fA 4 0.2425 19.2 0.956 Contact Map
2ks1B 1 0.0899 13.8 0.959 Contact Map
2j69A 1 0.376 12.2 0.96 Contact Map
2kncB 1 0.0899 11.5 0.96 Contact Map
4aybC 1 0.2044 11.3 0.96 Contact Map
2l2tA 2 0.0872 10.7 0.961 Contact Map
2m20A 2 0.0681 9.2 0.962 Contact Map
3c5yA 5 0.2316 9.2 0.962 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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