GREMLIN Database
DUF432 - Protein of unknown function (DUF432)
PFAM: PF04254 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 123 (123)
Sequences: 714 (549)
Seq/√Len: 49.5
META: 0.88

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
14_I78_S4.7291.00
15_E77_K3.8061.00
29_T96_E3.3581.00
62_T73_C2.9761.00
32_L97_L2.8871.00
13_L62_T2.7451.00
33_T92_E2.6821.00
17_E77_K2.6601.00
27_S100_R2.6031.00
50_L53_I2.5501.00
97_L112_V2.3451.00
22_L99_I2.1161.00
99_I109_V2.1111.00
33_T76_W1.8810.99
17_E79_E1.8740.99
33_T81_Y1.8600.99
20_I80_V1.7100.98
34_F93_G1.6170.98
3_N37_E1.5840.97
5_P9_T1.5790.97
3_N9_T1.5350.97
11_Y71_V1.5170.97
73_C111_K1.5070.97
16_F64_Y1.4900.96
51_L113_V1.4810.96
38_I110_S1.4420.96
33_T79_E1.3670.94
40_V54_F1.3480.94
107_V110_S1.3460.94
16_F112_V1.3420.93
39_G50_L1.3370.93
20_I86_D1.3330.93
9_T115_P1.3130.93
61_Y74_R1.2920.92
64_Y113_V1.2850.92
1_P5_P1.2790.92
63_L72_I1.2690.91
6_K9_T1.2390.90
25_G75_Y1.2350.90
52_D64_Y1.2270.90
23_E106_W1.2090.89
51_L84_I1.1780.88
63_L74_R1.1580.87
29_T123_Y1.1520.87
47_D87_V1.1500.87
30_I66_S1.1090.85
88_D91_R1.1030.84
37_E64_Y1.1000.84
66_S69_S1.0890.84
82_T92_E1.0850.83
29_T67_P1.0820.83
55_S73_C1.0670.82
2_V5_P1.0670.82
45_K123_Y1.0600.82
54_F64_Y1.0520.81
95_M112_V1.0440.81
109_V121_I1.0440.81
19_P66_S1.0410.81
73_C116_A1.0260.80
3_N10_N1.0210.79
29_T100_R1.0050.78
38_I90_L0.9850.77
37_E66_S0.9770.76
52_D113_V0.9670.76
61_Y64_Y0.9580.75
59_P103_S0.9510.74
102_T112_V0.9470.74
34_F85_P0.9370.73
57_V91_R0.9320.73
114_F119_M0.9280.72
7_E10_N0.9110.71
54_F101_N0.9040.71
10_N66_S0.9000.70
67_P122_Y0.8860.69
79_E82_T0.8840.69
11_Y31_Y0.8700.68
76_W115_P0.8590.67
10_N96_E0.8580.66
38_I45_K0.8580.66
30_I99_I0.8550.66
22_L26_S0.8540.66
28_A112_V0.8530.66
39_G53_I0.8320.64
103_S123_Y0.8310.64
17_E80_V0.8170.63
13_L73_C0.8040.61
75_Y115_P0.8020.61
45_K87_V0.7980.61
22_L118_G0.7940.60
55_S62_T0.7870.60
18_K79_E0.7840.59
65_G70_G0.7820.59
37_E74_R0.7780.59
42_L51_L0.7730.58
60_K74_R0.7730.58
16_F63_L0.7700.58
75_Y105_E0.7690.58
114_F117_Y0.7610.57
34_F119_M0.7610.57
13_L16_F0.7570.57
12_L35_P0.7450.56
23_E26_S0.7420.55
76_W116_A0.7330.54
69_S104_D0.7230.53
64_Y101_N0.7220.53
77_K95_M0.7040.51
51_L72_I0.6960.51
37_E61_Y0.6950.50
88_D119_M0.6940.50
94_V106_W0.6940.50
43_S47_D0.6890.50
42_L105_E0.6890.50
37_E55_S0.6870.50
30_I59_P0.6800.49
15_E75_Y0.6760.49
81_Y99_I0.6740.48
67_P79_E0.6720.48
4_L7_E0.6710.48
30_I114_F0.6680.48
17_E26_S0.6620.47
34_F41_F0.6600.47
16_F60_K0.6520.46
6_K14_I0.6490.46
38_I113_V0.6490.46
78_S81_Y0.6470.46
18_K93_G0.6460.45
102_T110_S0.6450.45
6_K59_P0.6440.45
32_L78_S0.6360.44
26_S102_T0.6340.44
44_S109_V0.6300.44
37_E54_F0.6270.44
106_W113_V0.6260.43
57_V111_K0.6250.43
43_S111_K0.6220.43
117_Y120_K0.6210.43
50_L71_V0.6210.43
23_E47_D0.6170.43
20_I48_Y0.6160.42
66_S115_P0.6140.42
25_G39_G0.6120.42
16_F42_L0.6100.42
9_T116_A0.6050.41
8_I23_E0.6040.41
91_R116_A0.6030.41
89_P122_Y0.5930.40
104_D122_Y0.5890.40
20_I82_T0.5880.40
42_L45_K0.5870.40
49_E67_P0.5860.40
22_L120_K0.5840.39
17_E103_S0.5800.39
88_D93_G0.5800.39
22_L28_A0.5790.39
25_G86_D0.5770.39
18_K109_V0.5740.38
25_G104_D0.5740.38
37_E60_K0.5670.38
12_L47_D0.5640.37
91_R110_S0.5620.37
93_G121_I0.5600.37
68_E91_R0.5580.37
93_G99_I0.5510.36
4_L86_D0.5480.36
31_Y122_Y0.5480.36
27_S45_K0.5450.36
34_F37_E0.5440.35
28_A51_L0.5420.35
112_V120_K0.5400.35
84_I99_I0.5390.35
98_T107_V0.5390.35
31_Y101_N0.5360.35
9_T12_L0.5360.35
16_F79_E0.5350.35
57_V66_S0.5320.34
105_E108_E0.5270.34
27_S95_M0.5230.34
14_I49_E0.5220.33
52_D101_N0.5040.32
84_I94_V0.5040.32
20_I30_I0.5030.32
2_V9_T0.5020.32
67_P113_V0.5020.32
64_Y74_R0.5000.31
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3nrfA 3 0.4634 13.9 0.921 Contact Map
4b9pA 1 0.3902 12.7 0.922 Contact Map
4q6kA 4 0.374 7.3 0.931 Contact Map
4b9cA 1 0.374 5.8 0.934 Contact Map
2xbtA 1 0.3902 5.5 0.935 Contact Map
1g43A 1 0.3821 5 0.936 Contact Map
3zqxA 1 0.3496 4.3 0.938 Contact Map
3zucA 1 0.3821 4.2 0.938 Contact Map
3pcoB 2 0.935 4.2 0.938 Contact Map
4p72A 2 0.935 4 0.939 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.006 seconds.