GREMLIN Database
DUF423 - Protein of unknown function (DUF423)
PFAM: PF04241 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 86 (86)
Sequences: 25250 (16243)
Seq/√Len: 1751.6
META: 0.904

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
21_E25_R4.2751.00
27_Q67_Y3.6621.00
25_R29_Y2.8931.00
65_S81_T2.8691.00
33_A55_F2.7461.00
6_G20_F2.2471.00
76_W79_A2.2301.00
18_A21_E2.1021.00
50_W53_W2.0221.00
19_V66_L1.8191.00
3_G26_Y1.8041.00
16_A19_V1.7751.00
5_F9_G1.7361.00
69_L78_G1.6651.00
47_L50_W1.5721.00
29_Y32_L1.5381.00
24_V27_Q1.4951.00
2_L20_F1.4931.00
19_V69_L1.4891.00
37_V55_F1.4571.00
61_L79_A1.4371.00
49_R53_W1.3831.00
50_W54_L1.3021.00
34_L56_L1.2591.00
46_R50_W1.2451.00
68_L72_T1.2301.00
30_H55_F1.1871.00
36_A39_L1.1601.00
22_T25_R1.1541.00
27_Q59_I1.1481.00
79_A83_I1.1411.00
34_L59_I1.1361.00
77_L80_I1.0931.00
40_L43_P1.0551.00
51_A55_F1.0381.00
28_M32_L1.0301.00
47_L51_A1.0291.00
38_G52_G1.0251.00
32_L36_A1.0221.00
53_W57_A1.0101.00
16_A70_A0.9971.00
16_A73_G0.9911.00
31_A59_I0.9901.00
59_I63_S0.9861.00
37_V51_A0.9421.00
14_L70_A0.9311.00
18_A25_R0.9301.00
48_L51_A0.9241.00
43_P48_L0.9161.00
30_H33_A0.9151.00
30_H59_I0.9101.00
39_L48_L0.9101.00
37_V52_G0.9071.00
23_A70_A0.9061.00
7_A22_T0.8851.00
19_V70_A0.8791.00
23_A63_S0.8751.00
24_V28_M0.8341.00
34_L38_G0.8311.00
57_A61_L0.8161.00
14_L17_L0.8021.00
10_L20_F0.7961.00
34_L37_V0.7731.00
45_S51_A0.7721.00
61_L65_S0.7701.00
66_L69_L0.7671.00
42_R45_S0.7631.00
37_V48_L0.7611.00
68_L74_I0.7611.00
34_L52_G0.7551.00
44_P49_R0.7531.00
20_F24_V0.7411.00
52_G56_L0.7281.00
35_L38_G0.7271.00
46_R49_R0.7241.00
31_A38_G0.7231.00
9_G13_R0.7221.00
78_G83_I0.7171.00
74_I77_L0.7071.00
35_L39_L0.7031.00
40_L48_L0.7011.00
41_A44_P0.7001.00
24_V31_A0.6991.00
56_L59_I0.6951.00
45_S48_L0.6931.00
68_L77_L0.6871.00
72_T75_R0.6851.00
33_A36_A0.6781.00
44_P48_L0.6741.00
36_A40_L0.6631.00
28_M31_A0.6591.00
41_A48_L0.6571.00
31_A35_L0.6511.00
22_T26_Y0.6471.00
2_L24_V0.6451.00
72_T77_L0.6441.00
82_P85_G0.6391.00
20_F23_A0.6361.00
18_A22_T0.6351.00
69_L81_T0.6341.00
53_W56_L0.6241.00
34_L55_F0.6211.00
72_T79_A0.6191.00
9_G12_G0.6181.00
45_S49_R0.6151.00
69_L75_R0.6061.00
61_L83_I0.5991.00
65_S69_L0.5951.00
17_L21_E0.5901.00
13_R17_L0.5901.00
20_F27_Q0.5881.00
37_V40_L0.5801.00
12_G17_L0.5781.00
44_P47_L0.5761.00
59_I64_G0.5761.00
68_L71_L0.5741.00
54_L57_A0.5741.00
26_Y66_L0.5561.00
11_K18_A0.5481.00
6_G9_G0.5481.00
66_L70_A0.5421.00
56_L60_V0.5361.00
24_V29_Y0.5351.00
5_F10_L0.5281.00
65_S80_I0.5201.00
16_A71_L0.5141.00
5_F69_L0.5111.00
11_K15_S0.5111.00
13_R16_A0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1v54K 1 0.5 9.6 0.874 Contact Map
2y69K 1 0.5 4.4 0.893 Contact Map
2e84A 1 0.186 1.7 0.914 Contact Map
2cvcA 1 0.1395 1.5 0.916 Contact Map
1z1nX 1 0.1279 1.3 0.919 Contact Map
4q2eA 2 1 1.1 0.924 Contact Map
4o9uB 2 0.7674 1 0.925 Contact Map
2q1fA 1 0.2209 0.9 0.927 Contact Map
4ru0A 1 0.186 0.9 0.927 Contact Map
2losA 1 0.7791 0.8 0.929 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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