GREMLIN Database
DUF422 - Protein of unknown function (DUF422)
PFAM: PF04240 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 208 (193)
Sequences: 6004 (4757)
Seq/√Len: 342.4
META: 0.877

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
105_A153_L3.5851.00
102_A150_L3.0001.00
79_L109_M2.7811.00
38_F42_L2.7181.00
40_A148_V2.5361.00
106_A150_L2.3741.00
195_L198_P2.2321.00
106_A146_F2.1721.00
55_H129_E2.1711.00
39_A43_L2.1101.00
42_L46_R2.0221.00
134_Y142_F2.0181.00
50_P140_S1.9891.00
182_F186_F1.9791.00
102_A106_A1.9701.00
42_L65_F1.9611.00
106_A110_V1.9581.00
178_L181_L1.9271.00
175_P179_Y1.9141.00
109_M149_G1.8791.00
144_G148_V1.8661.00
179_Y182_F1.8361.00
102_A154_A1.8321.00
150_L154_A1.8051.00
55_H127_I1.7551.00
12_A16_H1.7451.00
110_V146_F1.7411.00
58_D63_K1.7381.00
196_W199_A1.7321.00
6_V9_G1.7161.00
182_F185_F1.6921.00
33_A152_L1.6571.00
139_L143_L1.6471.00
40_A51_F1.6461.00
13_L16_H1.6431.00
87_A104_L1.6191.00
30_L77_F1.5951.00
50_P144_G1.5761.00
29_F33_A1.5441.00
17_A26_A1.5121.00
112_W116_L1.4941.00
178_L182_F1.4921.00
46_R66_G1.4701.00
113_D141_N1.4161.00
83_S109_M1.4141.00
179_Y183_L1.4081.00
83_S108_L1.4001.00
43_L47_T1.3861.00
7_L11_A1.3711.00
176_L179_Y1.3541.00
175_P182_F1.3491.00
38_F73_P1.3381.00
140_S144_G1.3331.00
125_F128_W1.3121.00
53_H129_E1.3111.00
156_L160_L1.2961.00
33_A77_F1.2951.00
179_Y186_F1.2941.00
181_L185_F1.2941.00
24_R28_L1.2921.00
32_I155_L1.2851.00
107_L146_F1.2801.00
80_A109_M1.2801.00
191_L199_A1.2771.00
154_A158_L1.2571.00
175_P178_L1.2531.00
186_F190_N1.2351.00
34_F77_F1.2251.00
57_T127_I1.2241.00
9_G12_A1.2161.00
13_L17_A1.1941.00
10_A13_L1.1921.00
173_L177_A1.1741.00
84_Y153_L1.1741.00
174_L177_A1.1731.00
80_A153_L1.1721.00
9_G17_A1.1711.00
181_L186_F1.1621.00
14_L17_A1.1541.00
167_A170_A1.1471.00
98_R101_R1.1461.00
143_L147_L1.1221.00
194_G199_A1.1151.00
195_L199_A1.1131.00
83_S87_A1.1071.00
91_L104_L1.1061.00
174_L178_L1.0971.00
121_V137_V1.0951.00
14_L18_V1.0921.00
10_A16_H1.0771.00
175_P186_F1.0741.00
190_N195_L1.0671.00
180_L184_L1.0651.00
181_L184_L1.0601.00
15_L18_V1.0561.00
42_L68_V1.0541.00
32_I36_I1.0521.00
59_T63_K1.0431.00
80_A152_L1.0301.00
56_Y72_I1.0241.00
176_L180_L1.0221.00
121_V124_G1.0191.00
22_G26_A1.0171.00
87_A105_A1.0121.00
38_F65_F1.0101.00
118_P121_V1.0041.00
178_L185_F1.0001.00
185_F189_L1.0001.00
40_A151_V0.9951.00
37_G152_L0.9921.00
9_G13_L0.9881.00
18_V23_W0.9811.00
109_M153_L0.9781.00
110_V149_G0.9721.00
16_H81_Y0.9711.00
157_R160_L0.9671.00
86_L108_L0.9631.00
37_G148_V0.9441.00
103_L146_F0.9441.00
128_W137_V0.9431.00
190_N193_F0.9381.00
105_A157_R0.9361.00
40_A50_P0.9341.00
97_R101_R0.9341.00
129_E132_G0.9321.00
101_R157_R0.9321.00
177_A181_L0.9281.00
189_L193_F0.9251.00
62_P70_L0.9231.00
36_I148_V0.9201.00
88_R101_R0.9151.00
184_L187_L0.9131.00
36_I152_L0.9091.00
194_G197_L0.9091.00
44_G54_Y0.9071.00
194_G198_P0.8881.00
103_L150_L0.8841.00
117_D137_V0.8751.00
112_W120_A0.8731.00
158_L161_R0.8731.00
84_Y157_R0.8681.00
185_F190_N0.8651.00
83_S153_L0.8641.00
79_L112_W0.8601.00
57_T124_G0.8551.00
173_L181_L0.8461.00
47_T50_P0.8451.00
59_T125_F0.8421.00
108_L111_A0.8411.00
40_A144_G0.8411.00
13_L18_V0.8391.00
182_F187_L0.8291.00
121_V128_W0.8251.00
178_L186_F0.8201.00
97_R100_L0.8171.00
183_L186_F0.8031.00
186_F189_L0.8031.00
9_G14_L0.8001.00
173_L176_L0.8001.00
38_F68_V0.7981.00
180_L187_L0.7901.00
172_L177_A0.7871.00
53_H130_D0.7851.00
180_L183_L0.7801.00
44_G76_W0.7801.00
113_D126_W0.7791.00
182_F189_L0.7781.00
6_V10_A0.7781.00
181_L188_G0.7781.00
178_L183_L0.7731.00
149_G153_L0.7711.00
116_L120_A0.7711.00
172_L175_P0.7701.00
185_F188_G0.7631.00
184_L188_G0.7591.00
106_A153_L0.7581.00
71_L74_L0.7531.00
175_P181_L0.7511.00
75_A78_M0.7501.00
174_L181_L0.7491.00
25_R28_L0.7441.00
35_L39_A0.7400.99
19_R22_G0.7270.99
45_V66_G0.7250.99
173_L178_L0.7220.99
34_F73_P0.7200.99
190_N198_P0.7180.99
177_A180_L0.7140.99
60_L63_K0.7140.99
153_L157_R0.7110.99
36_I39_A0.7100.99
33_A36_I0.7070.99
36_I40_A0.7070.99
179_Y190_N0.7040.99
133_G139_L0.7040.99
36_I155_L0.7020.99
73_P120_A0.6990.99
23_W27_L0.6950.99
174_L182_F0.6950.99
175_P185_F0.6940.99
84_Y88_R0.6870.99
9_G16_H0.6850.99
30_L33_A0.6810.99
6_V13_L0.6790.99
12_A15_L0.6770.99
27_L31_A0.6760.99
172_L181_L0.6750.99
45_V48_G0.6730.99
46_R68_V0.6690.99
11_A14_L0.6690.99
33_A37_G0.6690.99
172_L176_L0.6660.99
168_R171_A0.6630.99
183_L187_L0.6620.99
28_L32_I0.6610.99
41_E54_Y0.6610.99
22_G25_R0.6580.99
177_A182_F0.6530.99
140_S143_L0.6530.99
29_F156_L0.6520.99
54_Y113_D0.6510.99
157_R161_R0.6500.99
49_F53_H0.6490.99
187_L190_N0.6470.99
59_T62_P0.6450.99
183_L189_L0.6420.99
181_L190_N0.6410.99
181_L189_L0.6410.99
151_V154_A0.6290.98
163_R166_R0.6200.98
80_A149_G0.6200.98
173_L179_Y0.6150.98
17_A81_Y0.6100.98
116_L119_V0.6080.98
117_D136_G0.6080.98
141_N145_W0.6030.98
64_L70_L0.6030.98
172_L180_L0.6020.98
193_F196_W0.6000.98
42_L45_V0.5990.98
120_A125_F0.5960.98
121_V127_I0.5930.98
49_F147_L0.5910.98
192_A195_L0.5820.97
39_A42_L0.5800.97
45_V68_V0.5780.97
56_Y113_D0.5760.97
177_A184_L0.5750.97
37_G77_F0.5750.97
34_F37_G0.5740.97
174_L186_F0.5740.97
154_A157_R0.5740.97
9_G18_V0.5730.97
51_F76_W0.5700.97
63_K70_L0.5690.97
61_G71_L0.5680.97
6_V12_A0.5680.97
177_A186_F0.5670.97
119_V123_L0.5640.97
135_F198_P0.5630.97
191_L194_G0.5630.97
43_L50_P0.5610.97
137_V142_F0.5610.97
183_L188_G0.5600.97
169_R172_L0.5590.97
6_V14_L0.5560.96
49_F140_S0.5510.96
72_I76_W0.5470.96
176_L181_L0.5450.96
44_G50_P0.5430.96
102_A157_R0.5410.96
63_K66_G0.5390.96
12_A17_A0.5370.96
188_G192_A0.5350.96
179_Y185_F0.5340.96
43_L49_F0.5340.96
8_L15_L0.5310.95
179_Y187_L0.5310.95
88_R157_R0.5310.95
128_W138_P0.5280.95
158_L162_R0.5210.95
118_P144_G0.5210.95
96_R99_W0.5200.95
114_L117_D0.5140.95
115_V118_P0.5130.95
51_F144_G0.5120.94
173_L186_F0.5070.94
72_I120_A0.5040.94
156_L159_L0.5040.94
113_D142_F0.5000.94
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4qncA 2 0.3365 4.6 0.949 Contact Map
4x5mA 2 0.3462 3.1 0.953 Contact Map
1afoA 2 0.1154 2.6 0.955 Contact Map
3gd8A 4 0.4327 2.2 0.956 Contact Map
2jwaA 2 0.1202 2.1 0.957 Contact Map
2a65A 2 0.4327 1.9 0.958 Contact Map
2ks1B 1 0.125 1.7 0.959 Contact Map
4rngA 2 0.3413 1.6 0.959 Contact Map
2l2tA 2 0.1202 1.3 0.962 Contact Map
3j9tb 1 0 1.2 0.962 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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