GREMLIN Database
DUF417 - Protein of unknown function, DUF417
PFAM: PF04224 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 175 (143)
Sequences: 2048 (855)
Seq/√Len: 71.5
META: 0.808

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
115_S166_L3.6471.00
84_Y88_Y3.4661.00
97_I117_L3.3921.00
48_N132_T3.0341.00
100_L110_L2.8671.00
55_L86_F2.7081.00
23_V102_A2.5731.00
18_L162_A2.5561.00
43_V47_A2.3641.00
14_G168_A2.2211.00
23_V27_L2.2041.00
158_V162_A2.1911.00
118_A163_S2.1841.00
111_G166_L2.1531.00
40_E43_V2.1481.00
21_G161_G2.0451.00
119_V164_L2.0341.00
86_F90_L2.0221.00
36_A40_E1.8761.00
97_I121_M1.8511.00
116_L120_G1.7901.00
104_G111_G1.7901.00
17_L164_L1.7241.00
92_V96_L1.7071.00
100_L114_G1.6741.00
119_V160_L1.6100.99
115_S167_L1.5780.99
25_V158_V1.5770.99
26_L122_F1.5310.99
111_G115_S1.4810.99
26_L121_M1.4500.99
121_M124_T1.4470.99
146_L150_G1.4210.99
60_S82_G1.3990.98
116_L167_L1.3780.98
24_I157_V1.3760.98
17_L161_G1.3640.98
34_F92_V1.3590.98
22_L162_A1.3480.98
126_L130_F1.3330.98
36_A84_Y1.2900.97
112_A167_L1.2850.97
115_S119_V1.2500.97
57_P82_G1.2340.96
147_S150_G1.2330.96
39_A87_S1.2120.96
32_L37_Y1.2050.96
27_L95_I1.2000.96
15_Y165_W1.1640.95
94_E152_F1.1570.95
119_V167_L1.1350.94
59_F82_G1.1260.94
135_V150_G1.1200.94
103_L107_S1.1140.94
45_L153_L1.1100.93
32_L139_S1.0980.93
18_L158_V1.0910.93
57_P85_G1.0820.93
20_Y105_P1.0620.92
136_W141_G1.0610.92
93_V121_M1.0570.92
127_S131_T1.0560.92
27_L98_A1.0540.92
120_G124_T1.0470.91
31_G95_I1.0440.91
95_I99_L1.0400.91
43_V83_V1.0340.91
55_L89_L1.0280.91
140_L145_A1.0270.90
148_G151_Q1.0250.90
97_I118_A1.0170.90
42_I49_S1.0170.90
93_V97_I1.0030.89
46_V86_F0.9990.89
93_V124_T0.9990.89
25_V29_I0.9860.89
85_G89_L0.9860.89
9_R12_R0.9620.87
98_A102_A0.9590.87
37_Y140_L0.9550.87
52_M128_F0.9510.87
39_A42_I0.9440.86
29_I151_Q0.9280.85
102_A105_P0.9260.85
118_A122_F0.9240.85
34_F95_I0.9200.85
14_G164_L0.9180.85
88_Y92_V0.9060.84
154_L158_V0.9030.84
28_W32_L0.9000.84
34_F88_Y0.8880.83
104_G170_S0.8830.82
112_A171_A0.8570.81
35_T41_G0.8560.80
19_R166_L0.8530.80
21_G26_L0.8490.80
104_G169_D0.8470.80
82_G85_G0.8460.80
116_L119_V0.8440.79
113_L117_L0.8410.79
19_R105_P0.8360.79
97_I124_T0.8330.79
104_G107_S0.8230.78
42_I128_F0.8200.77
104_G166_L0.8190.77
13_L16_H0.8150.77
26_L124_T0.8150.77
59_F85_G0.8000.76
107_S110_L0.7990.76
40_E83_V0.7850.74
112_A116_L0.7840.74
24_I28_W0.7800.74
29_I158_V0.7760.74
35_T98_A0.7750.73
21_G29_I0.7740.73
46_V52_M0.7620.72
58_V82_G0.7610.72
49_S54_W0.7600.72
96_L100_L0.7600.72
29_I146_L0.7530.71
54_W58_V0.7520.71
99_L103_L0.7460.71
137_V151_Q0.7430.70
20_Y102_A0.7410.70
29_I154_L0.7330.69
37_Y137_V0.7290.69
27_L102_A0.7280.69
123_L160_L0.7160.67
38_E87_S0.7160.67
9_R13_L0.7130.67
50_P133_P0.7100.67
90_L167_L0.7070.66
41_G148_G0.7050.66
22_L49_S0.7010.66
20_Y165_W0.6970.65
13_L164_L0.6920.65
129_L135_V0.6920.65
131_T135_V0.6900.65
137_V140_L0.6850.64
136_W144_P0.6850.64
45_L149_A0.6790.63
28_W98_A0.6760.63
19_R115_S0.6710.62
32_L146_L0.6680.62
132_T151_Q0.6660.62
18_L161_G0.6650.62
36_A88_Y0.6640.62
32_L145_A0.6590.61
136_W142_G0.6560.61
20_Y161_G0.6560.61
94_E122_F0.6510.60
18_L21_G0.6450.59
93_V96_L0.6430.59
26_L29_I0.6410.59
101_L118_A0.6400.59
20_Y23_V0.6280.57
25_V159_L0.6270.57
135_V149_A0.6250.57
105_P111_G0.6140.56
20_Y115_S0.6120.55
97_I120_G0.6110.55
11_E172_K0.6010.54
137_V145_A0.6010.54
49_S131_T0.5980.54
86_F93_V0.5980.54
59_F89_L0.5980.54
42_I46_V0.5960.53
32_L140_L0.5940.53
105_P170_S0.5860.52
58_V152_F0.5840.52
55_L59_F0.5820.52
32_L137_V0.5810.52
165_W169_D0.5790.51
18_L22_L0.5750.51
93_V114_G0.5750.51
32_L148_G0.5740.51
44_P149_A0.5690.50
82_G86_F0.5680.50
39_A47_A0.5670.50
12_R109_K0.5650.50
84_Y87_S0.5650.50
52_M98_A0.5640.50
35_T145_A0.5610.49
49_S132_T0.5570.49
11_E14_G0.5570.49
90_L93_V0.5540.48
102_A112_A0.5540.48
23_V101_L0.5520.48
25_V146_L0.5510.48
19_R22_L0.5490.48
129_L150_G0.5460.47
13_L17_L0.5460.47
100_L111_G0.5390.46
26_L118_A0.5390.46
104_G108_P0.5370.46
11_E169_D0.5350.46
40_E98_A0.5320.46
28_W154_L0.5290.45
28_W148_G0.5280.45
167_L170_S0.5260.45
90_L121_M0.5150.44
30_G34_F0.5130.43
114_G117_L0.5120.43
46_V56_Y0.5100.43
31_G145_A0.5100.43
14_G26_L0.5090.43
17_L21_G0.5080.43
108_P166_L0.5050.42
101_L115_S0.5050.42
53_S108_P0.5040.42
149_A154_L0.5030.42
146_L151_Q0.5020.42
95_I106_W0.5010.42
101_L104_G0.5000.42
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3rkoL 1 0.7029 7.2 0.963 Contact Map
2fyuK 1 0.3029 5.6 0.965 Contact Map
3wu2Z 1 0.32 3.9 0.968 Contact Map
4he8L 1 0.7029 3.4 0.969 Contact Map
4ezcA 3 0.6286 3 0.969 Contact Map
4bwzA 2 0.3714 2.7 0.97 Contact Map
3tx3A 2 0.6686 2.2 0.971 Contact Map
4q25A 2 0.1771 2.2 0.972 Contact Map
3wajA 1 0.7486 1.6 0.974 Contact Map
4o93A 2 0.48 1.5 0.974 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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