GREMLIN Database
DUF413 - Protein of unknown function, DUF
PFAM: PF04219 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 90 (88)
Sequences: 503 (298)
Seq/√Len: 31.7
META: 0.527

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
33_M65_L4.1651.00
32_A41_R3.5061.00
22_E71_K3.3781.00
50_H59_R2.9871.00
4_D12_F2.4520.99
32_A48_E2.2830.99
61_P66_E2.1970.99
35_A41_R2.1440.98
54_V66_E1.9720.97
13_S23_A1.9190.97
33_M51_F1.8890.96
46_E49_K1.7010.94
7_N76_I1.6980.94
37_E73_L1.6560.93
35_A40_E1.6500.93
53_A58_E1.6030.92
34_N38_S1.5260.89
54_V70_L1.5100.89
29_Y32_A1.4860.88
10_R15_S1.4820.88
32_A35_A1.4620.87
43_P52_V1.4610.87
18_F26_L1.4280.86
54_V61_P1.4210.86
38_S49_K1.3890.84
43_P48_E1.3200.81
5_D19_T1.2610.78
27_E32_A1.2350.76
13_S24_Q1.2320.76
73_L77_R1.2310.76
4_D7_N1.2270.76
49_K53_A1.2090.75
4_D72_Y1.1640.72
27_E41_R1.1620.72
82_F85_L1.1380.70
25_L68_A1.1290.70
15_S18_F1.0990.68
25_L63_T1.0670.65
25_L65_L1.0610.65
49_K62_E1.0530.64
5_D10_R1.0130.61
64_P68_A0.9920.60
8_F18_F0.9510.56
12_F72_Y0.9440.56
53_A67_K0.9340.55
81_R87_G0.9250.54
79_P83_H0.9070.53
67_K88_S0.9000.52
17_D75_R0.8940.52
4_D9_P0.8930.52
43_P62_E0.8800.50
21_K24_Q0.8670.49
10_R22_E0.8660.49
83_H86_S0.8640.49
82_F86_S0.8590.49
53_A59_R0.8560.49
41_R48_E0.8470.48
30_G69_W0.8440.48
76_I82_F0.8440.48
41_R44_E0.8260.46
8_F33_M0.8250.46
36_L52_V0.8220.46
50_H63_T0.8200.46
74_K78_R0.8100.45
43_P51_F0.8090.45
41_R55_C0.8020.44
78_R86_S0.8010.44
10_R19_T0.8000.44
14_R37_E0.7970.44
9_P45_T0.7960.44
57_G64_P0.7910.43
12_F79_P0.7640.41
83_H88_S0.7610.41
54_V63_T0.7600.41
49_K67_K0.7550.40
75_R78_R0.7480.40
2_F8_F0.7460.40
26_L53_A0.7360.39
80_K87_G0.7220.38
54_V57_G0.7190.38
56_R63_T0.7180.38
37_E55_C0.7060.37
2_F14_R0.6940.36
31_V63_T0.6910.35
82_F87_G0.6690.34
16_G30_G0.6650.34
85_L88_S0.6570.33
34_N37_E0.6490.32
59_R62_E0.6470.32
60_E67_K0.6430.32
22_E39_G0.6410.32
6_K43_P0.6190.30
82_F88_S0.6130.30
15_S24_Q0.6130.30
47_E63_T0.6080.30
8_F12_F0.6060.29
50_H54_V0.6040.29
35_A80_K0.6040.29
52_V55_C0.6010.29
3_Y24_Q0.6010.29
21_K56_R0.6010.29
20_I23_A0.5840.28
7_N12_F0.5820.28
46_E70_L0.5790.28
48_E51_F0.5770.27
1_R31_V0.5760.27
71_K75_R0.5740.27
81_R86_S0.5720.27
9_P57_G0.5680.27
11_G16_G0.5660.27
11_G69_W0.5660.27
84_T87_G0.5630.27
63_T66_E0.5630.27
46_E50_H0.5600.26
8_F17_D0.5590.26
35_A42_E0.5580.26
2_F71_K0.5540.26
19_T81_R0.5530.26
63_T69_W0.5500.26
55_C73_L0.5490.26
39_G47_E0.5480.26
61_P70_L0.5450.25
61_P74_K0.5420.25
9_P61_P0.5360.25
39_G48_E0.5350.25
44_E60_E0.5340.25
29_Y41_R0.5320.25
25_L61_P0.5320.25
14_R67_K0.5200.24
50_H66_E0.5140.24
50_H62_E0.5130.24
10_R16_G0.5000.23
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2qdjA 1 0.4 38.4 0.899 Contact Map
4eljA 1 0.4222 18 0.914 Contact Map
3mwpA 2 0.9333 15.6 0.917 Contact Map
4o6iA 2 0.9333 13.9 0.919 Contact Map
2efvA 2 0.5 13.4 0.919 Contact Map
3q7cA 1 0.9333 13.2 0.919 Contact Map
4k7eA 2 0.6 11.8 0.921 Contact Map
4gveA 1 0.9111 11.3 0.922 Contact Map
3e6pL 1 0.3222 6.8 0.929 Contact Map
2wviA 1 0.6778 6.4 0.93 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.005 seconds.