GREMLIN Database
Alpha-E - A predicted alpha-helical domain with a conserved ER motif.
PFAM: PF04168 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 293 (292)
Sequences: 13996 (9310)
Seq/√Len: 544.8
META: 0.844

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
252_G260_Y3.5161.00
166_D271_H3.3291.00
257_R280_R3.2151.00
10_F285_S3.1031.00
151_L227_L2.7771.00
147_R230_H2.7621.00
56_R74_D2.7331.00
257_R276_E2.6231.00
196_R213_D2.5731.00
61_T64_N2.5421.00
158_A281_L2.4531.00
255_R259_R2.4501.00
228_E255_R2.4131.00
70_L122_L2.3761.00
52_A68_F2.3411.00
147_R233_A2.3091.00
253_R257_R2.2571.00
111_E124_R2.2141.00
232_A248_E2.2051.00
202_T205_R2.1831.00
262_D265_E2.1821.00
189_A216_F2.1701.00
228_E232_A2.1471.00
101_M136_T2.0771.00
45_A50_L2.0691.00
73_P84_R2.0541.00
256_A260_Y2.0421.00
162_A220_L2.0361.00
214_P225_D1.9511.00
101_M132_F1.9161.00
221_A225_D1.9101.00
211_L220_L1.9061.00
276_E280_R1.8921.00
252_G256_A1.8381.00
197_Y208_D1.8331.00
250_L287_A1.8131.00
193_Y205_R1.8001.00
180_W206_V1.7811.00
13_G149_L1.7781.00
54_A60_A1.7541.00
143_D195_R1.7531.00
154_R223_C1.7271.00
207_A211_L1.6881.00
225_D255_R1.6571.00
141_T192_A1.6541.00
68_F72_D1.6531.00
226_R229_E1.6051.00
90_A97_L1.5841.00
154_R226_R1.5841.00
212_L266_L1.5831.00
27_R126_K1.5701.00
286_D290_R1.5531.00
41_R45_A1.5501.00
283_A286_D1.5361.00
204_A264_D1.5321.00
192_A209_F1.5321.00
98_S139_T1.5201.00
221_A259_R1.5201.00
250_L283_A1.5171.00
271_H275_D1.5151.00
162_A278_Q1.5071.00
90_A105_L1.5011.00
247_A291_T1.4941.00
287_A290_R1.4801.00
251_A284_L1.4581.00
220_L277_L1.4571.00
48_G53_F1.4361.00
50_L54_A1.4351.00
251_A255_R1.4161.00
16_L132_F1.4121.00
150_R230_H1.4001.00
84_R88_E1.3941.00
207_A263_I1.3901.00
187_A210_L1.3861.00
136_T149_L1.3751.00
245_S248_E1.3581.00
17_E157_R1.3531.00
70_L79_I1.3491.00
53_F60_A1.3391.00
228_E251_A1.3351.00
253_R280_R1.3321.00
150_R154_R1.3311.00
224_L255_R1.3281.00
227_L251_A1.3271.00
124_R128_R1.3151.00
52_A56_R1.3101.00
229_E233_A1.3081.00
161_T187_A1.3081.00
224_L254_L1.3061.00
9_L149_L1.3031.00
166_D275_D1.2971.00
249_R260_Y1.2961.00
201_I206_V1.2891.00
254_L284_L1.2841.00
56_R72_D1.2791.00
57_Y64_N1.2701.00
21_N160_N1.2701.00
108_L128_R1.2601.00
269_G272_E1.2571.00
7_D293_F1.2421.00
12_L90_A1.2411.00
107_E127_E1.2291.00
249_R252_G1.2291.00
287_A291_T1.2281.00
41_R54_A1.2281.00
214_P221_A1.2281.00
123_D127_E1.2231.00
16_L129_S1.2171.00
85_A88_E1.2071.00
263_I267_L1.2071.00
161_T220_L1.2021.00
162_A277_L1.2011.00
166_D278_Q1.1951.00
280_R283_A1.1951.00
48_G51_E1.1951.00
12_L97_L1.1851.00
228_E248_E1.1761.00
41_R50_L1.1761.00
163_R278_Q1.1671.00
224_L281_L1.1651.00
193_Y201_I1.1621.00
283_A287_A1.1591.00
83_L112_L1.1591.00
154_R222_F1.1551.00
158_A220_L1.1521.00
73_P80_L1.1251.00
78_S81_S1.1201.00
52_A55_E1.1191.00
150_R226_R1.1161.00
83_L108_L1.1121.00
9_L145_G1.1111.00
151_L288_I1.1101.00
246_E250_L1.1051.00
197_Y205_R1.0961.00
68_F75_N1.0821.00
107_E111_E1.0791.00
84_R113_R1.0741.00
80_L112_L1.0711.00
97_L101_M1.0691.00
54_A58_G1.0671.00
250_L284_L1.0651.00
161_T218_R1.0641.00
162_A274_L1.0551.00
207_A267_L1.0441.00
289_S293_F1.0431.00
275_D278_Q1.0411.00
191_E195_R1.0391.00
181_E190_F1.0381.00
193_Y202_T1.0351.00
11_W15_Y1.0291.00
168_K179_Q1.0241.00
142_R146_W1.0171.00
104_A127_E1.0141.00
193_Y209_F1.0121.00
47_L50_L1.0061.00
264_D267_L1.0031.00
214_P259_R1.0021.00
19_A82_C1.0011.00
254_L280_R0.9931.00
99_S191_E0.9931.00
39_E42_P0.9881.00
228_E252_G0.9881.00
261_A273_F0.9871.00
44_L60_A0.9841.00
104_A135_L0.9791.00
246_E290_R0.9791.00
16_L86_A0.9781.00
150_R153_R0.9751.00
104_A131_L0.9751.00
44_L53_F0.9711.00
235_A248_E0.9691.00
49_D52_A0.9681.00
246_E262_D0.9621.00
45_A48_G0.9611.00
180_W203_P0.9561.00
137_D146_W0.9551.00
53_F68_F0.9541.00
159_D163_R0.9461.00
81_S85_A0.9441.00
112_L121_F0.9381.00
155_L284_L0.9341.00
111_E128_R0.9331.00
226_R230_H0.9271.00
32_L185_R0.9271.00
278_Q282_N0.9261.00
64_N67_R0.9251.00
284_L288_I0.9231.00
67_R71_F0.9211.00
244_S248_E0.9211.00
245_S291_T0.9191.00
62_A65_V0.9171.00
47_L51_E0.9151.00
40_W48_G0.9141.00
27_R31_R0.9131.00
105_L132_F0.9131.00
46_A51_E0.9071.00
87_R106_N0.9051.00
229_E232_A0.9051.00
208_D212_L0.8961.00
275_D279_A0.8951.00
50_L53_F0.8831.00
80_L109_Y0.8791.00
40_W51_E0.8781.00
11_W14_R0.8741.00
247_A250_L0.8731.00
225_D228_E0.8731.00
44_L49_D0.8731.00
80_L113_R0.8711.00
26_L70_L0.8691.00
158_A223_C0.8681.00
63_E67_R0.8681.00
250_L280_R0.8661.00
169_Y203_P0.8651.00
67_R72_D0.8611.00
277_L281_L0.8581.00
204_A267_L0.8581.00
20_E24_R0.8561.00
141_T213_D0.8501.00
133_S153_R0.8491.00
53_F57_Y0.8491.00
282_N286_D0.8481.00
286_D289_S0.8481.00
87_R109_Y0.8441.00
151_L234_L0.8421.00
133_S138_E0.8391.00
104_A128_R0.8381.00
53_F64_N0.8381.00
246_E249_R0.8361.00
88_E91_R0.8351.00
276_E279_A0.8341.00
158_A224_L0.8331.00
225_D259_R0.8321.00
285_S289_S0.8271.00
130_A135_L0.8261.00
47_L53_F0.8201.00
137_D150_R0.8191.00
19_A129_S0.8161.00
250_L253_R0.8111.00
211_L274_L0.8101.00
243_R248_E0.8021.00
27_R123_D0.8001.00
151_L230_H0.7981.00
266_L269_G0.7971.00
137_D153_R0.7931.00
159_D162_A0.7831.00
159_D278_Q0.7821.00
44_L51_E0.7821.00
125_V129_S0.7821.00
8_N89_N0.7781.00
261_A265_E0.7741.00
123_D126_K0.7721.00
59_E64_N0.7711.00
168_K172_L0.7701.00
159_D282_N0.7651.00
261_A266_L0.7631.00
164_L186_S0.7601.00
98_S101_M0.7531.00
205_R208_D0.7521.00
104_A132_F0.7491.00
58_G61_T0.7491.00
129_S133_S0.7491.00
170_H271_H0.7371.00
235_A245_S0.7361.00
208_D213_D0.7331.00
165_L274_L0.7301.00
263_I266_L0.7291.00
20_E218_R0.7251.00
157_R218_R0.7231.00
230_H233_A0.7221.00
8_N92_A0.7211.00
38_E41_R0.7191.00
37_A41_R0.7171.00
197_Y213_D0.7151.00
238_Y241_G0.7141.00
15_Y85_A0.7131.00
40_W43_L0.7121.00
77_S82_C0.7121.00
166_D170_H0.7101.00
111_E114_E0.7101.00
41_R60_A0.7081.00
252_G255_R0.7051.00
51_E54_A0.7041.00
168_K183_L0.6991.00
86_A105_L0.6961.00
144_E147_R0.6921.00
235_A292_Y0.6861.00
29_N32_L0.6861.00
70_L121_F0.6851.00
65_V75_N0.6851.00
42_P50_L0.6821.00
120_E124_R0.6821.00
227_L284_L0.6811.00
170_H275_D0.6801.00
48_G52_A0.6781.00
248_E252_G0.6781.00
212_L261_A0.6761.00
160_N164_L0.6721.00
249_R262_D0.6721.00
133_S137_D0.6711.00
40_W47_L0.6701.00
261_A267_L0.6701.00
279_A282_N0.6701.00
43_L48_G0.6681.00
103_E123_D0.6671.00
33_L36_P0.6641.00
143_D215_D0.6641.00
7_D11_W0.6591.00
54_A57_Y0.6581.00
141_T196_R0.6551.00
146_W153_R0.6551.00
247_A287_A0.6531.00
85_A89_N0.6521.00
19_A83_L0.6471.00
224_L227_L0.6441.00
239_G242_G0.6441.00
257_R261_A0.6421.00
100_E188_S0.6421.00
231_L247_A0.6391.00
249_R256_A0.6381.00
254_L257_R0.6291.00
209_F216_F0.6291.00
96_R145_G0.6281.00
43_L46_A0.6271.00
44_L48_G0.6261.00
38_E45_A0.6231.00
139_T188_S0.6221.00
42_P45_A0.6191.00
73_P113_R0.6181.00
13_G153_R0.6161.00
165_L210_L0.6141.00
137_D142_R0.6091.00
80_L84_R0.6071.00
165_L211_L0.6051.00
250_L254_L0.6041.00
144_E234_L0.6041.00
154_R227_L0.6031.00
15_Y89_N0.6011.00
185_R190_F0.5971.00
17_E218_R0.5961.00
71_F112_L0.5951.00
49_D77_S0.5951.00
141_T209_F0.5941.00
15_Y18_R0.5931.00
65_V68_F0.5921.00
268_E276_E0.5921.00
185_R188_S0.5921.00
90_A98_S0.5901.00
164_L187_A0.5881.00
75_N81_S0.5871.00
257_R273_F0.5840.99
232_A252_G0.5840.99
39_E48_G0.5820.99
224_L258_L0.5800.99
43_L50_L0.5800.99
22_T25_L0.5790.99
44_L52_A0.5780.99
127_E131_L0.5780.99
12_L132_F0.5760.99
257_R260_Y0.5750.99
249_R253_R0.5740.99
192_A216_F0.5710.99
20_E130_A0.5690.99
6_A148_F0.5680.99
72_D75_N0.5680.99
279_A283_A0.5680.99
138_E218_R0.5670.99
43_L47_L0.5660.99
72_D78_S0.5660.99
5_V93_V0.5660.99
220_L274_L0.5650.99
46_A52_A0.5640.99
221_A255_R0.5600.99
101_M135_L0.5590.99
253_R276_E0.5570.99
228_E259_R0.5560.99
207_A270_L0.5550.99
22_T160_N0.5530.99
37_A59_E0.5530.99
51_E55_E0.5510.99
82_C86_A0.5500.99
192_A195_R0.5490.99
169_Y180_W0.5490.99
5_V95_D0.5480.99
43_L53_F0.5480.99
42_P47_L0.5480.99
45_A49_D0.5450.99
222_F226_R0.5440.99
39_E46_A0.5440.99
39_E47_L0.5430.99
196_R215_D0.5430.99
145_G153_R0.5410.99
141_T215_D0.5410.99
76_P81_S0.5400.99
253_R260_Y0.5400.99
225_D229_E0.5390.99
23_A79_I0.5380.99
272_E279_A0.5370.99
23_A125_V0.5360.99
116_L119_P0.5310.99
106_N109_Y0.5290.99
214_P219_S0.5280.99
282_N285_S0.5270.99
158_A277_L0.5260.99
49_D53_F0.5250.99
231_L251_A0.5250.99
53_F61_T0.5240.99
101_M140_M0.5240.99
251_A256_A0.5230.99
55_E58_G0.5220.99
142_R150_R0.5220.99
89_N93_V0.5200.99
94_R102_W0.5200.99
147_R215_D0.5200.99
250_L255_R0.5180.99
260_Y264_D0.5180.99
20_E188_S0.5180.99
132_F136_T0.5170.99
168_K171_L0.5150.99
7_D289_S0.5150.99
266_L273_F0.5150.99
244_S252_G0.5150.99
159_D281_L0.5150.99
203_P207_A0.5140.99
133_S154_R0.5130.99
107_E128_R0.5120.99
17_E153_R0.5080.99
259_R262_D0.5050.99
12_L149_L0.5050.99
112_L125_V0.5050.99
95_D98_S0.5040.99
247_A251_A0.5040.99
161_T210_L0.5030.99
180_W193_Y0.5030.99
263_I269_G0.5030.99
245_S260_Y0.5030.99
109_Y112_L0.5020.98
133_S136_T0.5020.98
192_A196_R0.5000.98
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4ab7A 3 0.1809 14.8 0.961 Contact Map
2qx5A 1 0.471 7.2 0.966 Contact Map
1sumB 2 0.1741 6.8 0.966 Contact Map
1iapA 1 0.3276 5.8 0.967 Contact Map
1x3aA 1 0.116 5.6 0.968 Contact Map
1v9vA 1 0.2048 3.1 0.971 Contact Map
2dc3A 2 0.4334 2.7 0.972 Contact Map
4nboA 1 0.256 2.7 0.972 Contact Map
3sk3A 2 0.2048 2.6 0.973 Contact Map
1dvpA 2 0.4334 2.6 0.973 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0095 seconds.