GREMLIN Database
DUF373 - Domain of unknown function (DUF373)
PFAM: PF04123 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 335 (333)
Sequences: 1695 (1170)
Seq/√Len: 64.1
META: 0.875

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
29_N70_T7.8281.00
26_R57_D7.6141.00
37_A46_A4.6311.00
5_L69_A4.2711.00
11_D14_G4.1531.00
51_E129_V4.1341.00
37_A50_F3.8751.00
21_T72_T3.5581.00
28_A31_E3.3001.00
9_R82_D3.2951.00
100_G124_D3.2661.00
13_L23_V3.1661.00
11_D15_R3.1141.00
77_V81_A3.0531.00
49_I70_T3.0451.00
93_L98_A2.9571.00
101_A121_V2.9271.00
71_I89_L2.9021.00
51_E54_K2.8481.00
90_D120_R2.8311.00
267_P307_G2.7681.00
114_L123_V2.7411.00
13_L72_T2.6341.00
91_E95_K2.5711.00
19_I23_V2.5661.00
71_I85_I2.4561.00
36_L40_D2.4381.00
30_L53_I2.3871.00
26_R30_L2.3641.00
16_K36_L2.3531.00
55_I104_V2.3231.00
4_V59_L2.2971.00
21_T88_Q2.2751.00
84_K87_E2.2541.00
43_D46_A2.2341.00
58_E62_E2.2021.00
84_K88_Q2.1951.00
54_K58_E2.1471.00
15_R75_E2.0931.00
26_R53_I2.0521.00
31_E35_K2.0461.00
52_A68_V2.0191.00
16_K40_D2.0151.00
13_L32_A2.0091.00
86_A90_D1.9651.00
27_E31_E1.9641.00
82_D125_S1.9611.00
255_L259_L1.9581.00
238_G261_F1.9501.00
86_A120_R1.9101.00
227_A272_A1.8401.00
238_G265_S1.8261.00
93_L121_V1.8211.00
77_V109_E1.8021.00
93_L97_K1.7891.00
169_G186_L1.7881.00
2_T124_D1.7721.00
231_I272_A1.7671.00
29_N49_I1.7380.99
42_E127_R1.7280.99
74_D80_E1.7120.99
162_G196_G1.7080.99
17_A43_D1.6500.99
242_V261_F1.6170.99
5_L101_A1.5780.99
4_V55_I1.5420.99
19_I32_A1.5130.99
53_I57_D1.5110.99
36_L43_D1.5080.99
87_E91_E1.4790.98
101_A117_I1.4750.98
53_I68_V1.4130.98
11_D72_T1.4000.98
24_I95_K1.3930.98
56_Y66_V1.3840.97
111_E128_R1.3500.97
23_V32_A1.3490.97
30_L38_L1.3390.97
324_A330_G1.3150.96
132_K146_K1.3150.96
46_A50_F1.3140.96
168_Y184_I1.3070.96
6_C52_A1.3070.96
13_L49_I1.2940.96
12_D16_K1.2920.96
10_D45_D1.2850.96
40_D132_K1.2810.96
44_S131_V1.2750.96
17_A32_A1.2740.96
159_G197_F1.2670.96
35_K39_A1.2600.96
227_A276_A1.2430.95
34_V50_F1.2400.95
31_E34_V1.2360.95
11_D74_D1.2340.95
238_G264_G1.2310.95
93_L120_R1.2290.95
85_I113_V1.2260.95
251_L255_L1.2240.95
76_K80_E1.2240.95
34_V38_L1.2140.94
145_K162_G1.2070.94
78_G81_A1.2040.94
116_I123_V1.2030.94
103_L125_S1.1940.94
241_S264_G1.1760.93
4_V66_V1.1650.93
68_V241_S1.1580.93
30_L57_D1.1240.92
150_D217_G1.1220.92
195_K200_D1.1120.91
223_T279_G1.1110.91
92_V98_A1.1100.91
74_D84_K1.1060.91
139_S142_Y1.1060.91
108_A133_Q1.0990.91
230_L275_V1.0970.91
102_I125_S1.0950.91
194_I200_D1.0930.91
69_A96_F1.0810.90
33_A50_F1.0810.90
277_L300_P1.0780.90
56_Y68_V1.0780.90
132_K147_L1.0770.90
79_V125_S1.0650.90
5_L71_I1.0570.89
3_L69_A1.0530.89
156_T159_G1.0520.89
324_A333_I1.0460.89
4_V102_I1.0420.89
130_V145_K1.0400.88
49_I108_A1.0390.88
321_S327_I1.0360.88
172_L181_L1.0290.88
141_Y195_K1.0280.88
8_D12_D1.0270.88
36_L46_A1.0260.88
109_E152_K1.0260.88
326_S329_A1.0240.88
9_R125_S1.0100.87
322_Y326_S1.0040.87
310_L313_A1.0020.87
310_L314_S1.0020.87
30_L34_V0.9940.86
60_K123_V0.9930.86
154_S161_P0.9900.86
90_D122_P0.9850.86
64_E102_I0.9740.85
55_I58_E0.9710.85
3_L101_A0.9690.85
44_S81_A0.9640.84
62_E80_E0.9550.84
154_S159_G0.9480.83
239_Y243_S0.9480.83
56_Y60_K0.9400.83
86_A116_I0.9390.83
110_D115_P0.9360.83
156_T161_P0.9320.82
1_R310_L0.9300.82
105_S195_K0.9280.82
270_V307_G0.9180.81
133_Q214_L0.9120.81
65_D191_Y0.9110.81
13_L17_A0.9090.81
111_E126_V0.9020.80
36_L107_G0.8970.80
128_R152_K0.8900.79
34_V37_A0.8870.79
53_I127_R0.8850.79
327_I330_G0.8850.79
71_I144_I0.8840.79
89_L117_I0.8830.79
325_L329_A0.8810.79
48_A52_A0.8730.78
120_R124_D0.8720.78
159_G196_G0.8660.77
161_P197_F0.8650.77
256_L259_L0.8620.77
263_Y315_N0.8620.77
303_V307_G0.8620.77
7_V313_A0.8610.77
91_E94_E0.8570.77
44_S133_Q0.8470.76
136_S139_S0.8450.76
28_A67_E0.8440.75
19_I43_D0.8440.75
165_L185_L0.8410.75
21_T84_K0.8380.75
162_G189_G0.8380.75
13_L70_T0.8270.74
167_I183_A0.8270.74
162_G193_L0.8260.74
16_K39_A0.8220.74
6_C48_A0.8220.74
324_A328_V0.8190.73
51_E55_I0.8190.73
178_G237_L0.8180.73
13_L19_I0.8150.73
71_I92_V0.8140.73
7_V10_D0.8060.72
284_E296_Y0.8040.72
187_V191_Y0.7970.71
155_R195_K0.7950.71
108_A142_Y0.7940.71
253_G256_L0.7930.71
74_D77_V0.7910.71
95_K116_I0.7900.71
88_Q132_K0.7880.70
54_K168_Y0.7880.70
113_V116_I0.7860.70
15_R23_V0.7850.70
11_D43_D0.7850.70
292_R295_R0.7840.70
12_D45_D0.7830.70
131_V273_G0.7810.70
154_S192_L0.7800.70
324_A329_A0.7770.69
252_S262_I0.7770.69
51_E127_R0.7750.69
198_G295_R0.7750.69
33_A37_A0.7730.69
7_V235_I0.7690.68
69_A101_A0.7620.68
118_Q123_V0.7620.68
322_Y330_G0.7560.67
183_A186_L0.7550.67
92_V205_R0.7550.67
24_I67_E0.7530.67
94_E228_I0.7530.67
149_E195_K0.7520.67
79_V219_I0.7520.67
42_E160_P0.7490.66
300_P303_V0.7490.66
78_G119_S0.7490.66
250_W255_L0.7460.66
4_V61_K0.7450.66
77_V88_Q0.7450.66
156_T160_P0.7410.66
133_Q142_Y0.7400.65
17_A40_D0.7400.65
164_L188_L0.7400.65
326_S330_G0.7380.65
192_L274_L0.7380.65
17_A39_A0.7340.65
60_K113_V0.7320.65
33_A36_L0.7320.65
17_A36_L0.7300.64
2_T100_G0.7290.64
193_L234_G0.7270.64
81_A109_E0.7240.64
238_G272_A0.7190.63
30_L36_L0.7190.63
62_E166_L0.7170.63
32_A35_K0.7170.63
16_K32_A0.7170.63
109_E153_Y0.7150.63
203_L208_S0.7130.63
250_W258_I0.7120.63
182_G200_D0.7110.62
163_L187_V0.7110.62
62_E173_L0.7110.62
220_S224_Y0.7100.62
43_D133_Q0.7090.62
132_K143_L0.7090.62
116_I130_V0.7090.62
29_N68_V0.7080.62
213_S280_R0.7010.61
6_C118_Q0.7000.61
265_S269_L0.6990.61
71_I162_G0.6940.61
7_V117_I0.6930.60
147_L196_G0.6920.60
257_L260_A0.6900.60
301_F307_G0.6880.60
150_D155_R0.6840.59
133_Q146_K0.6820.59
226_A229_L0.6800.59
242_V245_Y0.6780.59
233_V314_S0.6740.58
176_L179_Y0.6740.58
109_E141_Y0.6720.58
36_L45_D0.6720.58
5_L285_Y0.6700.58
100_G122_P0.6700.58
251_L257_L0.6650.57
321_S324_A0.6630.57
9_R77_V0.6630.57
17_A88_Q0.6600.57
19_I35_K0.6590.57
52_A116_I0.6570.56
274_L300_P0.6570.56
308_L312_G0.6560.56
5_L12_D0.6560.56
82_D115_P0.6530.56
144_I174_L0.6530.56
238_G242_V0.6510.56
60_K66_V0.6500.56
291_R296_Y0.6480.55
234_G265_S0.6480.55
71_I88_Q0.6450.55
77_V139_S0.6450.55
256_L260_A0.6450.55
88_Q141_Y0.6450.55
322_Y325_L0.6450.55
98_A197_F0.6440.55
33_A46_A0.6420.55
6_C9_R0.6420.55
203_L258_I0.6410.55
133_Q139_S0.6410.55
43_D51_E0.6390.54
43_D155_R0.6390.54
99_D183_A0.6370.54
11_D16_K0.6370.54
123_V148_L0.6360.54
142_Y160_P0.6350.54
234_G275_V0.6350.54
324_A327_I0.6350.54
307_G312_G0.6340.54
61_K91_E0.6330.54
49_I169_G0.6290.53
148_L191_Y0.6290.53
92_V96_F0.6280.53
309_I327_I0.6280.53
169_G182_G0.6280.53
79_V128_R0.6280.53
172_L182_G0.6270.53
65_D225_V0.6270.53
142_Y146_K0.6260.53
303_V309_I0.6250.53
41_P47_N0.6240.53
135_R139_S0.6230.52
90_D173_L0.6230.52
192_L233_V0.6220.52
3_L96_F0.6220.52
71_I130_V0.6210.52
234_G309_I0.6200.52
224_Y276_A0.6200.52
224_Y228_I0.6170.52
24_I320_L0.6160.52
43_D108_A0.6150.52
52_A56_Y0.6150.52
252_S274_L0.6130.51
111_E115_P0.6130.51
171_L304_L0.6130.51
7_V12_D0.6110.51
22_P25_G0.6110.51
4_V63_G0.6110.51
116_I258_I0.6100.51
251_L259_L0.6100.51
40_D107_G0.6090.51
112_S126_V0.6090.51
116_I306_L0.6080.51
7_V21_T0.6080.51
321_S328_V0.6050.50
205_R235_I0.6040.50
227_A279_G0.6040.50
67_E92_V0.6040.50
251_L258_I0.6040.50
273_G303_V0.6030.50
169_G185_L0.6010.50
134_S195_K0.6000.50
229_L233_V0.5980.50
4_V8_D0.5970.49
58_E89_L0.5960.49
154_S196_G0.5960.49
249_G252_S0.5950.49
102_I272_A0.5940.49
266_I314_S0.5940.49
311_Y314_S0.5930.49
65_D96_F0.5920.49
4_V56_Y0.5920.49
77_V152_K0.5910.49
203_L320_L0.5910.49
329_A332_L0.5910.49
160_P191_Y0.5900.49
52_A55_I0.5890.48
296_Y300_P0.5880.48
311_Y315_N0.5870.48
44_S110_D0.5860.48
70_T122_P0.5860.48
2_T165_L0.5860.48
255_L258_I0.5850.48
171_L180_A0.5850.48
114_L118_Q0.5840.48
244_S278_L0.5810.48
323_L330_G0.5810.48
21_T153_Y0.5800.47
38_L87_E0.5800.47
109_E142_Y0.5770.47
69_A99_D0.5760.47
22_P110_D0.5760.47
202_A206_F0.5760.47
162_G234_G0.5760.47
102_I227_A0.5760.47
250_W259_L0.5750.47
6_C279_G0.5740.47
152_K160_P0.5730.47
12_D73_G0.5710.46
115_P125_S0.5710.46
228_I277_L0.5640.46
96_F99_D0.5630.45
116_I157_F0.5630.45
273_G304_L0.5620.45
276_A299_L0.5600.45
52_A64_E0.5590.45
18_G65_D0.5580.45
34_V332_L0.5580.45
89_L110_D0.5580.45
30_L235_I0.5570.45
211_R215_Y0.5560.45
112_S146_K0.5560.45
157_F195_K0.5560.45
21_T149_E0.5560.45
252_S256_L0.5550.45
46_A133_Q0.5550.45
258_I261_F0.5550.45
142_Y305_A0.5530.44
71_I119_S0.5520.44
241_S245_Y0.5520.44
185_L275_V0.5500.44
307_G311_Y0.5500.44
48_A143_L0.5490.44
103_L117_I0.5480.44
313_A317_L0.5480.44
79_V144_I0.5470.44
151_P154_S0.5470.44
45_D107_G0.5470.44
92_V101_A0.5450.43
139_S143_L0.5440.43
146_K149_E0.5420.43
160_P268_W0.5420.43
124_D160_P0.5410.43
305_A312_G0.5410.43
37_A171_L0.5400.43
69_A92_V0.5400.43
23_V62_E0.5390.43
86_A122_P0.5380.43
116_I232_L0.5380.43
327_I331_I0.5380.43
198_G201_E0.5370.43
84_K201_E0.5370.43
17_A28_A0.5360.42
234_G238_G0.5350.42
19_I241_S0.5350.42
88_Q146_K0.5340.42
61_K274_L0.5340.42
53_I229_L0.5330.42
101_A119_S0.5330.42
37_A150_D0.5320.42
55_I79_V0.5300.42
264_G271_I0.5290.42
133_Q143_L0.5290.42
123_V281_L0.5280.41
24_I91_E0.5280.41
35_K57_D0.5250.41
230_L239_Y0.5250.41
165_L188_L0.5240.41
199_L312_G0.5240.41
273_G297_L0.5230.41
2_T31_E0.5230.41
165_L229_L0.5220.41
102_I115_P0.5210.41
76_K132_K0.5200.41
289_R292_R0.5200.41
33_A53_I0.5190.40
220_S223_T0.5180.40
109_E112_S0.5180.40
202_A207_Y0.5180.40
42_E82_D0.5180.40
24_I232_L0.5170.40
146_K160_P0.5170.40
9_R112_S0.5160.40
164_L186_L0.5160.40
176_L184_I0.5150.40
74_D85_I0.5150.40
72_T88_Q0.5150.40
12_D36_L0.5130.40
96_F131_V0.5120.40
99_D317_L0.5110.40
18_G27_E0.5110.40
292_R302_F0.5090.39
127_R230_L0.5070.39
87_E232_L0.5060.39
117_I239_Y0.5060.39
19_I56_Y0.5050.39
9_R115_P0.5050.39
33_A85_I0.5040.39
66_V133_Q0.5040.39
95_K101_A0.5040.39
93_L135_R0.5040.39
162_G226_A0.5040.39
81_A134_S0.5020.39
147_L268_W0.5020.39
10_D16_K0.5010.38
10_D14_G0.5010.38
68_V150_D0.5010.38
159_G193_L0.5000.38
69_A79_V0.5000.38
59_L63_G0.5000.38
214_L218_R0.5000.38
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2aifA 1 0.1672 31.6 0.97 Contact Map
4r3uC 1 0.3343 30.1 0.97 Contact Map
4j29A 2 0.209 28.5 0.97 Contact Map
3c8nA 2 0.2836 28.2 0.97 Contact Map
1fvpA 2 0.2716 27.1 0.971 Contact Map
3os4A 1 0.4388 26.9 0.971 Contact Map
1e2bA 1 0.2627 25.5 0.971 Contact Map
1jsxA 1 0.3493 24.2 0.971 Contact Map
4g84A 2 0.394 19.4 0.973 Contact Map
2gb4A 1 0.4269 18 0.973 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0102 seconds.