GREMLIN Database
DUF368 - Domain of unknown function (DUF368)
PFAM: PF04018 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 245 (236)
Sequences: 9128 (6421)
Seq/√Len: 417.9
META: 0.824

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
24_E53_N3.6251.00
44_G47_A3.5261.00
138_L186_P3.1141.00
144_A168_L3.1031.00
109_N112_T3.0631.00
49_W54_G2.9761.00
30_K203_S2.8111.00
115_A212_L2.6351.00
36_A48_F2.5271.00
76_S177_K2.4931.00
77_Y81_N2.3521.00
142_F189_L2.3071.00
203_S207_K2.2021.00
23_E210_H2.1791.00
5_D64_I2.1651.00
142_F186_P2.1501.00
172_I187_F2.1501.00
128_G226_N2.0671.00
38_K42_K2.0641.00
39_L44_G2.0601.00
46_K50_K2.0511.00
27_N203_S2.0421.00
73_K177_K2.0351.00
174_D178_D2.0301.00
76_S174_D2.0041.00
124_Y128_G1.9801.00
141_L187_F1.9501.00
204_W208_K1.9211.00
28_S53_N1.8951.00
119_G216_L1.8761.00
61_G65_L1.8681.00
36_A51_K1.8641.00
216_L220_F1.8561.00
149_I191_A1.8081.00
30_K200_R1.7461.00
175_A180_D1.7311.00
112_T211_D1.7011.00
33_N51_K1.7011.00
82_Y85_L1.6701.00
27_N207_K1.6451.00
62_L66_I1.6341.00
24_E28_S1.6241.00
66_I70_S1.5671.00
5_D152_M1.5611.00
142_F193_V1.5591.00
141_L183_V1.5481.00
84_I88_S1.5461.00
3_A67_S1.5331.00
125_L128_G1.5291.00
188_G192_V1.5251.00
76_S173_L1.5221.00
33_N36_A1.5221.00
115_A216_L1.5071.00
3_A7_V1.4971.00
39_L51_K1.4841.00
170_E174_D1.4771.00
59_P62_L1.4571.00
172_I183_V1.4561.00
147_I166_L1.4471.00
124_Y226_N1.4391.00
106_K211_D1.4361.00
77_Y82_Y1.4101.00
49_W55_N1.4081.00
28_S57_L1.4021.00
83_P87_W1.3781.00
41_L44_G1.3701.00
21_I60_L1.3581.00
168_L171_P1.3471.00
127_T226_N1.3441.00
76_S80_E1.3441.00
35_K51_K1.3421.00
102_G218_T1.3421.00
26_I203_S1.3381.00
91_F225_L1.3221.00
17_F214_L1.3141.00
139_L143_L1.3121.00
146_A193_V1.3061.00
125_L129_L1.3061.00
65_L69_L1.2791.00
134_T168_L1.2761.00
193_V197_L1.2571.00
140_F168_L1.2561.00
113_I117_L1.2561.00
115_A119_G1.2551.00
34_L38_K1.2481.00
1_M60_L1.2361.00
122_I125_L1.2321.00
40_L48_F1.2291.00
209_Y212_L1.2231.00
175_A183_V1.2141.00
23_E27_N1.2051.00
39_L43_G1.1961.00
180_D183_V1.1961.00
48_F52_I1.1811.00
182_L185_L1.1801.00
68_I172_I1.1781.00
112_T215_A1.1741.00
98_I222_L1.1581.00
37_L41_L1.1551.00
150_C198_F1.1541.00
45_F49_W1.1371.00
210_H214_L1.1371.00
55_N59_P1.1361.00
138_L142_F1.1181.00
104_Q210_H1.1131.00
50_K55_N1.1091.00
196_L200_R1.1061.00
111_K212_L1.1021.00
204_W209_Y1.0861.00
120_A124_Y1.0841.00
40_L45_F1.0681.00
38_K41_L1.0601.00
101_V218_T1.0591.00
186_P189_L1.0501.00
205_L213_T1.0441.00
141_L171_P1.0421.00
3_A71_L1.0391.00
89_F93_L1.0381.00
121_A125_L1.0321.00
6_V173_L1.0251.00
110_L114_L1.0171.00
146_A197_L1.0091.00
15_I19_T1.0011.00
72_A173_L1.0001.00
151_A162_I0.9941.00
94_I228_I0.9931.00
105_I108_W0.9841.00
32_I52_I0.9821.00
138_L182_L0.9791.00
31_S51_K0.9691.00
140_F144_A0.9631.00
112_T115_A0.9631.00
72_A177_K0.9581.00
33_N39_L0.9581.00
171_P183_V0.9571.00
108_W112_T0.9551.00
36_A39_L0.9411.00
39_L47_A0.9341.00
116_L120_A0.9221.00
86_T89_F0.9201.00
48_F51_K0.9161.00
185_L188_G0.9071.00
5_D151_A0.9051.00
106_K210_H0.9041.00
175_A184_L0.8991.00
144_A163_L0.8921.00
105_I112_T0.8841.00
141_L172_I0.8841.00
79_L86_T0.8801.00
116_L222_L0.8761.00
45_F48_F0.8751.00
69_L73_K0.8741.00
78_L82_Y0.8731.00
115_A118_I0.8721.00
141_L186_P0.8681.00
108_W113_I0.8671.00
111_K115_A0.8671.00
135_P168_L0.8631.00
6_V71_L0.8591.00
137_S140_F0.8571.00
79_L87_W0.8521.00
21_I56_F0.8521.00
174_D180_D0.8451.00
78_L86_T0.8421.00
18_I97_S0.8421.00
154_L217_L0.8381.00
35_K38_K0.8361.00
185_L189_L0.8351.00
136_D168_L0.8341.00
112_T212_L0.8321.00
35_K39_L0.8281.00
99_P102_G0.8251.00
123_A220_F0.8211.00
6_V68_I0.8211.00
181_L185_L0.8201.00
183_V186_P0.8181.00
6_V72_A0.8151.00
62_L65_L0.8121.00
36_A52_I0.8101.00
136_D171_P0.8091.00
114_L118_I0.8041.00
153_I195_L0.7981.00
54_G58_L0.7921.00
197_L201_L0.7831.00
27_N30_K0.7811.00
110_L113_I0.7801.00
123_A127_T0.7801.00
70_S74_L0.7791.00
47_A50_K0.7781.00
47_A51_K0.7731.00
68_I176_V0.7711.00
7_V10_V0.7711.00
188_G191_A0.7691.00
138_L183_V0.7681.00
187_F191_A0.7671.00
58_L62_L0.7661.00
133_E136_D0.7661.00
140_F143_L0.7651.00
104_Q214_L0.7621.00
16_A25_L0.7611.00
43_G47_A0.7531.00
95_L99_P0.7531.00
153_I199_S0.7501.00
74_L77_Y0.7491.00
92_G96_A0.7431.00
24_E56_F0.7311.00
144_A166_L0.7271.00
74_L78_L0.7251.00
174_D177_K0.7201.00
23_E206_L0.7181.00
101_V221_M0.7161.00
56_F60_L0.7141.00
176_V184_L0.7031.00
64_I152_M0.7001.00
67_S73_K0.7001.00
105_I215_A0.7001.00
189_L193_V0.6971.00
75_I86_T0.6971.00
162_I165_L0.6961.00
1_M64_I0.6911.00
148_A159_G0.6901.00
141_L168_L0.6871.00
124_Y127_T0.6791.00
107_K211_D0.6781.00
200_R203_S0.6771.00
206_L214_L0.6761.00
116_L218_T0.6751.00
153_I198_F0.6741.00
70_S73_K0.6741.00
27_N31_S0.6731.00
211_D215_A0.6701.00
149_I195_L0.6680.99
72_A76_S0.6680.99
74_L81_N0.6670.99
148_A191_A0.6660.99
53_N56_F0.6500.99
1_M5_D0.6500.99
102_G108_W0.6490.99
162_I166_L0.6490.99
134_T140_F0.6480.99
10_V15_I0.6480.99
33_N200_R0.6470.99
175_A178_D0.6460.99
225_L229_W0.6460.99
201_L204_W0.6450.99
172_I191_A0.6430.99
89_F96_A0.6410.99
118_I121_A0.6400.99
116_L119_G0.6400.99
17_F101_V0.6370.99
173_L177_K0.6370.99
1_M21_I0.6370.99
4_A12_G0.6370.99
103_K106_K0.6350.99
151_A159_G0.6280.99
153_I202_L0.6280.99
182_L186_P0.6250.99
88_S91_F0.6220.99
221_M226_N0.6210.99
1_M16_A0.6200.99
6_V67_S0.6180.99
29_I153_I0.6160.99
138_L189_L0.6140.99
207_K210_H0.6140.99
17_F217_L0.6090.99
40_L43_G0.6060.99
163_L168_L0.6060.99
4_A152_M0.6050.99
108_W215_A0.6040.99
65_L188_G0.6030.99
73_K77_Y0.6010.99
84_I87_W0.5990.99
80_E170_E0.5970.99
30_K196_L0.5970.99
86_T90_F0.5920.99
204_W207_K0.5870.99
214_L217_L0.5840.99
148_A152_M0.5840.99
157_I162_I0.5820.99
119_G123_A0.5820.99
49_W52_I0.5810.99
217_L221_M0.5810.99
212_L216_L0.5810.99
221_M227_K0.5790.99
67_S70_S0.5770.99
171_P175_A0.5770.99
222_L226_N0.5760.99
213_T217_L0.5760.99
34_L41_L0.5730.98
189_L192_V0.5720.98
59_P66_I0.5710.98
68_I173_L0.5710.98
233_E236_E0.5700.98
84_I231_W0.5700.98
5_D12_G0.5690.98
107_K112_T0.5680.98
98_I102_G0.5680.98
25_L29_I0.5660.98
43_G48_F0.5660.98
88_S229_W0.5640.98
200_R207_K0.5630.98
36_A41_L0.5610.98
1_M25_L0.5610.98
213_T216_L0.5600.98
5_D148_A0.5590.98
94_I225_L0.5590.98
197_L200_R0.5590.98
71_L74_L0.5580.98
121_A124_Y0.5560.98
73_K76_S0.5550.98
101_V104_Q0.5550.98
224_S227_K0.5540.98
148_A160_S0.5530.98
33_N38_K0.5510.98
48_F54_G0.5500.98
175_A179_L0.5490.98
36_A40_L0.5490.98
25_L153_I0.5470.98
191_A195_L0.5450.98
3_A6_V0.5440.98
143_L147_I0.5430.98
112_T119_G0.5420.98
224_S228_I0.5420.98
78_L89_F0.5390.98
28_S61_G0.5380.98
67_S71_L0.5380.98
72_A176_V0.5370.98
163_L187_F0.5360.98
75_I173_L0.5350.98
144_A147_I0.5320.98
143_L146_A0.5300.97
10_V94_I0.5280.97
144_A151_A0.5270.97
56_F59_P0.5270.97
176_V179_L0.5270.97
32_I54_G0.5250.97
152_M159_G0.5240.97
43_G46_K0.5200.97
198_F201_L0.5190.97
119_G127_T0.5190.97
32_I51_K0.5170.97
118_I122_I0.5170.97
26_I199_S0.5160.97
120_A222_L0.5150.97
109_N113_I0.5140.97
177_K180_D0.5140.97
148_A187_F0.5130.97
34_L200_R0.5110.97
192_V196_L0.5100.97
172_I175_A0.5080.97
122_I126_I0.5050.97
33_N37_L0.5030.97
206_L213_T0.5010.96
219_G223_G0.5000.96
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4f35D 2 0.5551 25.1 0.952 Contact Map
3wdoA 1 0.7592 24.5 0.952 Contact Map
2cfqA 1 0.751 13.2 0.957 Contact Map
1pw4A 1 0.8041 11.5 0.958 Contact Map
2mofA 1 0.1714 9.4 0.96 Contact Map
4pgrA 1 0.7224 8.6 0.961 Contact Map
4m64A 1 0.9755 5.4 0.964 Contact Map
4pypA 1 0.9102 5.1 0.965 Contact Map
2a65A 2 0.4122 4.5 0.966 Contact Map
3o7qA 1 0.7592 4.4 0.966 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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