GREMLIN Database
DUF349 - Domain of Unknown Function (DUF349)
PFAM: PF03993 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 75 (73)
Sequences: 30432 (18167)
Seq/√Len: 2126.3
META: 0.873

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
41_E65_E2.3041.00
41_E58_E1.8641.00
61_E65_E1.8501.00
34_K69_I1.8091.00
58_E61_E1.7841.00
34_K37_A1.7811.00
30_E33_E1.7781.00
25_D28_R1.7641.00
37_A41_E1.7561.00
30_E34_K1.7531.00
32_L36_E1.7051.00
37_A40_E1.6221.00
29_E33_E1.4941.00
34_K65_E1.4931.00
33_E36_E1.4851.00
40_E44_A1.4371.00
33_E37_A1.4361.00
36_E40_E1.3701.00
17_R21_F1.3671.00
23_E26_E1.3601.00
3_W7_R1.3231.00
12_A16_R1.3171.00
5_R9_A1.3161.00
40_E43_E1.3061.00
64_E68_E1.3051.00
65_E68_E1.2811.00
39_I43_E1.2591.00
26_E30_E1.2431.00
60_K63_Q1.2341.00
7_R11_D1.2161.00
27_E30_E1.2121.00
9_A12_A1.2101.00
41_E44_A1.1971.00
42_A62_L1.1591.00
51_D54_A1.1501.00
56_A60_K1.1481.00
26_E29_E1.1351.00
23_E27_E1.0981.00
54_A58_E1.0861.00
31_N69_I1.0781.00
53_K57_K1.0761.00
61_E64_E1.0671.00
42_A59_L1.0421.00
45_L58_E1.0241.00
12_A15_E0.9891.00
19_E23_E0.9821.00
13_F17_R0.9781.00
20_F24_L0.9781.00
16_R19_E0.9711.00
63_Q67_K0.9631.00
57_K61_E0.9411.00
24_L28_R0.9331.00
15_E19_E0.9181.00
60_K64_E0.9101.00
48_S55_A0.9101.00
8_A12_A0.9091.00
52_W56_A0.9081.00
52_W55_A0.8731.00
4_K8_A0.8591.00
22_A26_E0.8541.00
28_R71_P0.8501.00
11_D14_F0.8461.00
54_A57_K0.8441.00
10_C14_F0.8351.00
18_K22_A0.8271.00
11_D15_E0.8111.00
38_L69_I0.8081.00
44_A47_E0.8071.00
58_E65_E0.8011.00
36_E39_I0.8001.00
58_E62_L0.7531.00
32_L72_V0.7461.00
19_E22_A0.7391.00
45_L62_L0.7391.00
38_L62_L0.7361.00
35_K69_I0.7301.00
20_F23_E0.7261.00
24_L27_E0.7221.00
59_L63_Q0.7201.00
14_F18_K0.7121.00
32_L73_P0.7111.00
7_R10_C0.7081.00
48_S58_E0.7081.00
55_A58_E0.7031.00
45_L55_A0.6571.00
28_R31_N0.6491.00
21_F25_D0.6421.00
31_N34_K0.6351.00
15_E18_K0.6341.00
46_A49_E0.6311.00
18_K21_F0.6271.00
3_W10_C0.6131.00
49_E55_A0.6101.00
41_E62_L0.6061.00
56_A59_L0.5931.00
31_N35_K0.5901.00
13_F16_R0.5861.00
28_R73_P0.5711.00
3_W14_F0.5691.00
31_N72_V0.5661.00
36_E43_E0.5561.00
57_K60_K0.5461.00
22_A25_D0.5421.00
21_F24_L0.5381.00
48_S54_A0.5321.00
8_A11_D0.5171.00
49_E52_W0.5171.00
43_E46_A0.5151.00
44_A48_S0.5101.00
64_E67_K0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1sesA 2 1 15.5 0.811 Contact Map
2dq3A 2 0.96 10.3 0.825 Contact Map
3ieeA 1 1 10 0.826 Contact Map
3qneA 2 0.9867 9.7 0.827 Contact Map
2dq0A 2 0.9467 9.7 0.827 Contact Map
1wleA 2 0.96 7.9 0.834 Contact Map
2wl8A 1 1 6.6 0.84 Contact Map
4cfgA 2 0.9867 6.1 0.842 Contact Map
2d4yA 1 0.7867 5.9 0.844 Contact Map
4yfkF 1 0.9067 5.8 0.844 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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