GREMLIN Database
DUF328 - Protein of unknown function (DUF328)
PFAM: PF03883 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 232 (232)
Sequences: 11757 (8033)
Seq/√Len: 527.4
META: 0.756

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
30_E145_R4.3281.00
89_D92_S3.4701.00
78_L98_L2.8141.00
94_S97_D2.7221.00
206_R210_E2.7071.00
97_D210_E2.6341.00
47_A54_D2.5361.00
100_Y104_H2.4381.00
60_N63_R2.3881.00
203_L225_E2.3361.00
52_I56_L2.2821.00
13_F79_A2.2441.00
62_E66_A2.2261.00
144_D148_D2.2231.00
26_E149_A2.1931.00
61_V65_Q2.1561.00
3_I208_I2.1311.00
81_T91_A2.1041.00
37_V41_L2.1021.00
97_D212_R2.0941.00
146_I167_A2.0941.00
58_E62_E2.0931.00
185_E190_Y2.0881.00
137_N140_A2.0621.00
215_D218_D2.0231.00
93_L206_R2.0151.00
157_V208_I2.0061.00
10_T81_T1.9791.00
101_A209_I1.9751.00
17_L74_K1.9591.00
42_S45_D1.9541.00
47_A57_A1.8951.00
4_L156_L1.8901.00
43_P58_E1.8201.00
72_N122_Q1.8041.00
27_F149_A1.7251.00
149_A152_E1.6841.00
35_I64_F1.6241.00
143_G169_D1.6231.00
151_N174_K1.6131.00
155_V176_R1.5851.00
37_V132_L1.5811.00
5_L84_V1.5701.00
38_L64_F1.5681.00
169_D172_A1.5521.00
192_V197_A1.5461.00
181_V192_V1.5371.00
81_T86_D1.5201.00
217_E220_K1.5171.00
54_D58_E1.5131.00
102_Q118_L1.5051.00
33_E141_F1.4871.00
218_D221_G1.4761.00
28_L31_A1.4341.00
77_I88_L1.4331.00
213_I218_D1.4251.00
88_L205_A1.4181.00
74_K79_A1.4161.00
97_D209_I1.4131.00
165_F170_P1.4121.00
37_V134_N1.3991.00
203_L224_L1.3931.00
5_L105_L1.3771.00
43_P61_V1.3731.00
2_L106_R1.3651.00
128_M167_A1.3591.00
98_L118_L1.3451.00
106_R114_L1.3191.00
30_E141_F1.3021.00
33_E37_V1.3001.00
210_E224_L1.2911.00
77_I98_L1.2861.00
5_L205_A1.2811.00
83_D195_F1.2801.00
144_D169_D1.2761.00
208_I219_L1.2641.00
31_A67_W1.2621.00
73_A120_L1.2461.00
144_D172_A1.2261.00
32_E36_D1.2191.00
27_F31_A1.2181.00
93_L210_E1.2141.00
220_K229_F1.2121.00
100_Y212_R1.2061.00
162_N166_K1.2051.00
92_S206_R1.2051.00
87_G199_K1.1831.00
114_L149_A1.1751.00
52_I57_A1.1731.00
30_E136_R1.1691.00
93_L98_L1.1691.00
12_D15_T1.1601.00
99_D103_D1.1581.00
59_L63_R1.1561.00
12_D81_T1.1551.00
147_T169_D1.1521.00
77_I90_A1.1441.00
185_E188_G1.1231.00
183_K190_Y1.1201.00
207_Y222_F1.1201.00
30_E33_E1.1141.00
84_V159_L1.1121.00
38_L41_L1.1041.00
96_A99_D1.0941.00
151_N175_A1.0891.00
37_V135_G1.0861.00
12_D74_K1.0641.00
34_L134_N1.0631.00
100_Y209_I1.0621.00
37_V40_K1.0501.00
93_L97_D1.0501.00
31_A35_I1.0431.00
3_I205_A1.0411.00
148_D172_A1.0281.00
45_D48_K1.0221.00
90_A93_L1.0211.00
154_E176_R1.0191.00
185_E189_K1.0191.00
116_R119_D1.0171.00
170_P177_V1.0161.00
13_F74_K1.0061.00
147_T172_A1.0051.00
165_F177_V0.9981.00
147_T151_N0.9971.00
184_D196_F0.9971.00
33_E36_D0.9931.00
4_L165_F0.9911.00
157_V180_P0.9891.00
105_L205_A0.9881.00
2_L154_E0.9841.00
41_L45_D0.9831.00
208_I213_I0.9791.00
139_Y166_K0.9781.00
155_V216_P0.9741.00
162_N179_T0.9741.00
77_I101_A0.9721.00
35_I111_L0.9671.00
73_A122_Q0.9661.00
159_L204_M0.9661.00
95_E99_D0.9521.00
22_H75_P0.9501.00
205_A209_I0.9481.00
93_L101_A0.9451.00
147_T168_V0.9441.00
143_G148_D0.9421.00
33_E135_G0.9341.00
88_L93_L0.9331.00
54_D57_A0.9331.00
207_Y221_G0.9321.00
112_Y122_Q0.9321.00
106_R149_A0.9261.00
86_D199_K0.9171.00
146_I168_V0.9151.00
37_V133_P0.9121.00
32_E69_T0.9061.00
15_T74_K0.9061.00
23_T102_Q0.9051.00
145_R148_D0.8911.00
68_S122_Q0.8901.00
183_K192_V0.8901.00
105_L209_I0.8861.00
46_L52_I0.8851.00
74_K91_A0.8781.00
214_T218_D0.8711.00
77_I117_P0.8611.00
106_R117_P0.8591.00
199_K203_L0.8591.00
8_A11_L0.8571.00
94_S210_E0.8491.00
197_A201_R0.8481.00
96_A212_R0.8411.00
203_L207_Y0.8371.00
158_N179_T0.8371.00
10_T86_D0.8331.00
22_H120_L0.8321.00
26_E116_R0.8271.00
108_L114_L0.8261.00
25_P28_L0.8241.00
35_I39_R0.8241.00
8_A81_T0.8191.00
43_P54_D0.8181.00
21_K99_D0.8061.00
195_F199_K0.8011.00
55_K58_E0.8001.00
92_S210_E0.7961.00
186_K196_F0.7951.00
194_S197_A0.7941.00
44_A47_A0.7931.00
196_F199_K0.7911.00
15_T18_P0.7851.00
190_Y230_D0.7831.00
29_D33_E0.7791.00
28_L32_E0.7791.00
8_A86_D0.7761.00
131_K137_N0.7621.00
207_Y224_L0.7561.00
115_L121_I0.7551.00
72_N120_L0.7491.00
31_A111_L0.7451.00
38_L132_L0.7451.00
158_N165_F0.7431.00
128_M163_E0.7411.00
157_V219_L0.7401.00
49_L130_T0.7361.00
220_K231_E0.7351.00
83_D86_D0.7321.00
207_Y211_N0.7301.00
50_M64_F0.7281.00
173_L177_V0.7271.00
83_D199_K0.7261.00
86_D195_F0.7251.00
27_F30_E0.7231.00
44_A48_K0.7141.00
207_Y210_E0.7121.00
101_A105_L0.7091.00
62_E65_Q0.7081.00
186_K193_I0.7081.00
24_Q28_L0.7041.00
106_R150_L0.6991.00
46_L61_V0.6971.00
83_D194_S0.6941.00
101_A205_A0.6941.00
63_R66_A0.6861.00
98_L102_Q0.6821.00
186_K189_K0.6821.00
156_L175_A0.6811.00
90_A98_L0.6751.00
14_E17_L0.6741.00
41_L46_L0.6701.00
110_G128_M0.6701.00
207_Y213_I0.6671.00
155_V178_I0.6661.00
161_S164_Y0.6661.00
35_I67_W0.6641.00
88_L206_R0.6611.00
22_H70_A0.6601.00
184_D193_I0.6581.00
154_E174_K0.6561.00
181_V184_D0.6561.00
78_L90_A0.6551.00
75_P78_L0.6531.00
26_E31_A0.6451.00
228_R231_E0.6411.00
110_G127_E0.6401.00
12_D16_P0.6391.00
43_P57_A0.6381.00
60_N64_F0.6341.00
24_Q27_F0.6271.00
35_I46_L0.6261.00
43_P47_A0.6251.00
170_P179_T0.6241.00
13_F121_I0.6191.00
199_K225_E0.6181.00
147_T170_P0.6171.00
216_P220_K0.6171.00
153_D176_R0.6141.00
186_K191_K0.6131.00
4_L150_L0.6091.00
36_D39_R0.6081.00
40_K135_G0.6071.00
187_N191_K0.6061.00
104_H214_T0.6061.00
44_A54_D0.6041.00
61_V64_F0.6041.00
18_P78_L0.6021.00
193_I196_F0.6021.00
3_I214_T0.5971.00
53_S56_L0.5961.00
108_L168_V0.5951.00
79_A121_I0.5951.00
178_I216_P0.5900.99
59_L62_E0.5890.99
29_D32_E0.5890.99
102_Q117_P0.5870.99
13_F17_L0.5860.99
83_D87_G0.5850.99
24_Q120_L0.5850.99
192_V195_F0.5840.99
141_F145_R0.5820.99
80_F109_S0.5820.99
13_F16_P0.5760.99
140_A143_G0.5750.99
13_F123_P0.5740.99
9_K127_E0.5720.99
90_A101_A0.5670.99
67_W122_Q0.5660.99
90_A206_R0.5650.99
16_P19_T0.5610.99
24_Q31_A0.5600.99
148_D152_E0.5590.99
36_D40_K0.5570.99
32_E68_S0.5560.99
64_F67_W0.5550.99
3_I157_V0.5550.99
80_F124_Y0.5540.99
18_P91_A0.5530.99
153_D175_A0.5530.99
19_T95_E0.5520.99
26_E29_D0.5520.99
35_I38_L0.5500.99
46_L50_M0.5480.99
58_E61_V0.5460.99
4_L168_V0.5450.99
75_P120_L0.5440.99
147_T175_A0.5440.99
41_L49_L0.5440.99
10_T55_K0.5440.99
21_K95_E0.5430.99
184_D227_Y0.5430.99
179_T192_V0.5420.99
93_L209_I0.5410.99
25_P31_A0.5400.99
10_T52_I0.5400.99
216_P219_L0.5390.99
177_V180_P0.5380.99
185_E228_R0.5380.99
223_D228_R0.5350.99
47_A52_I0.5340.99
162_N181_V0.5320.99
48_K51_G0.5260.99
89_D206_R0.5250.99
136_R145_R0.5250.99
24_Q29_D0.5240.99
31_A38_L0.5240.99
70_A120_L0.5230.99
143_G147_T0.5220.99
221_G224_L0.5220.99
46_L64_F0.5210.99
151_N173_L0.5210.99
207_Y218_D0.5210.99
134_N140_A0.5200.99
8_A85_Y0.5180.99
28_L69_T0.5170.99
86_D196_F0.5170.99
215_D220_K0.5170.99
104_H212_R0.5160.99
69_T122_Q0.5140.99
11_L79_A0.5140.99
26_E152_E0.5120.99
89_D93_L0.5120.99
55_K59_L0.5100.98
213_I216_P0.5080.98
108_L167_A0.5060.98
127_E164_Y0.5040.98
68_S71_E0.5040.98
17_L75_P0.5030.98
183_K230_D0.5010.98
3_I209_I0.5000.98
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4ou0A 1 0.2543 5.9 0.964 Contact Map
2fu2A 1 0.3103 4.9 0.965 Contact Map
2yfvC 1 0.2371 4.7 0.966 Contact Map
2m1bA 1 0.4095 4.4 0.966 Contact Map
4aaiA 2 0.2845 4.2 0.966 Contact Map
2m87A 1 0.4267 3.6 0.968 Contact Map
2hzmB 1 0.4655 3.5 0.968 Contact Map
3eyiA 1 0.2457 3.2 0.968 Contact Map
3bd1A 4 0.25 3.1 0.969 Contact Map
2cg4A 6 0.5819 3 0.969 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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