GREMLIN Database
NicO - High-affinity nickel-transport protein
PFAM: PF03824 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 282 (278)
Sequences: 7824 (5115)
Seq/√Len: 306.8
META: 0.787

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
140_D144_H4.3421.00
87_L236_G3.9411.00
266_G269_I3.5411.00
122_L125_R3.3301.00
141_H144_H2.8851.00
49_T146_Y2.7731.00
262_L269_I2.7531.00
101_R221_M2.6761.00
171_A174_A2.6761.00
262_L266_G2.5831.00
28_K32_A2.5121.00
258_A261_D2.3621.00
47_H51_V2.2931.00
86_L90_A2.2691.00
81_E84_S2.2081.00
229_T233_L2.2071.00
139_V148_V2.1951.00
38_R142_S2.1861.00
84_S88_I2.1831.00
114_D117_E2.0961.00
74_V78_I2.0431.00
17_F195_C2.0321.00
262_L265_L2.0211.00
267_Y271_G2.0001.00
251_G255_G1.9911.00
52_T222_T1.9691.00
13_L195_C1.9321.00
263_G266_G1.9231.00
55_A59_A1.8991.00
53_L150_L1.8801.00
239_L242_L1.8551.00
166_A169_A1.7941.00
198_A201_V1.7911.00
265_L269_I1.7861.00
57_A61_G1.7701.00
91_I95_L1.7701.00
257_L261_D1.7671.00
61_G65_L1.7611.00
90_A94_L1.7451.00
263_G267_Y1.7321.00
51_V55_A1.7231.00
163_H167_L1.7101.00
158_T161_E1.7021.00
244_A258_A1.6501.00
127_L130_R1.6471.00
109_R112_R1.5821.00
36_P39_V1.5481.00
120_H124_H1.5441.00
145_M148_V1.5331.00
116_A120_H1.5311.00
6_L10_T1.5291.00
51_V54_L1.4981.00
255_G258_A1.4921.00
4_L7_L1.4891.00
117_E120_H1.4871.00
146_Y149_G1.4781.00
121_L124_H1.4641.00
74_V77_V1.4361.00
156_F160_T1.4331.00
249_L254_W1.4111.00
138_L141_H1.4111.00
268_V271_G1.3961.00
255_G259_A1.3781.00
119_E123_A1.3771.00
156_F159_A1.3711.00
239_L243_L1.3651.00
71_L75_G1.3621.00
235_G239_L1.3511.00
72_H75_G1.3491.00
118_L121_L1.3411.00
225_G229_T1.3291.00
104_F108_R1.3231.00
227_S231_A1.3181.00
221_M225_G1.3171.00
14_R18_D1.3051.00
117_E121_L1.3021.00
264_L268_V1.3001.00
226_L229_T1.2971.00
135_L138_L1.2871.00
226_L233_L1.2851.00
97_L225_G1.2831.00
20_D23_K1.2791.00
119_E122_L1.2751.00
78_I82_V1.2741.00
89_I271_G1.2691.00
75_G78_I1.2681.00
252_G255_G1.2661.00
106_R112_R1.2621.00
250_S255_G1.2581.00
128_H134_P1.2531.00
87_L91_I1.2421.00
203_M207_Y1.2371.00
128_H132_F1.2361.00
240_L244_A1.2351.00
142_S145_M1.2321.00
144_H148_V1.2251.00
233_L237_I1.2141.00
151_L154_L1.2121.00
50_V150_L1.2101.00
116_A119_E1.1991.00
127_L131_L1.1991.00
83_A239_L1.1981.00
52_T189_A1.1961.00
167_L170_G1.1861.00
157_D160_T1.1861.00
69_S72_H1.1841.00
237_I241_G1.1841.00
256_A259_A1.1801.00
238_E242_L1.1751.00
154_L157_D1.1731.00
220_N223_I1.1691.00
153_G156_F1.1641.00
98_R102_R1.1631.00
18_D197_T1.1591.00
272_L276_T1.1581.00
12_G15_H1.1551.00
101_R105_R1.1551.00
225_G230_V1.1451.00
225_G233_L1.1411.00
59_A62_A1.1351.00
143_W146_Y1.1311.00
218_Y222_T1.1251.00
118_L122_L1.1201.00
97_L100_I1.1121.00
253_F256_A1.1021.00
140_D143_W1.0981.00
168_A172_A1.0961.00
105_R109_R1.0931.00
275_L279_V1.0881.00
217_L221_M1.0821.00
120_H123_A1.0791.00
28_K31_V1.0771.00
78_I81_E1.0741.00
135_L148_V1.0721.00
113_Y118_L1.0681.00
264_L267_Y1.0661.00
87_L90_A1.0621.00
43_F47_H1.0591.00
255_G261_D1.0581.00
183_L186_L1.0571.00
240_L254_W1.0501.00
139_V144_H1.0481.00
126_G129_A1.0451.00
58_L62_A1.0421.00
70_A73_M1.0401.00
94_L98_R1.0391.00
93_N271_G1.0371.00
126_G131_L1.0281.00
224_T229_T1.0261.00
149_G156_F1.0211.00
118_L124_H1.0201.00
17_F270_V1.0171.00
52_T55_A1.0121.00
156_F161_E1.0081.00
44_S51_V1.0031.00
118_L125_R1.0021.00
56_V59_A0.9941.00
133_G137_R0.9911.00
126_G132_F0.9851.00
155_G158_T0.9801.00
16_A195_C0.9791.00
131_L134_P0.9771.00
111_G114_D0.9741.00
166_A170_G0.9701.00
253_F257_L0.9671.00
44_S193_S0.9661.00
157_D161_E0.9641.00
56_V60_L0.9591.00
266_G273_F0.9581.00
232_L235_G0.9521.00
30_L35_R0.9451.00
115_E119_E0.9441.00
88_I92_G0.9421.00
56_V219_Y0.9411.00
260_L265_L0.9391.00
113_Y117_E0.9351.00
164_L167_L0.9321.00
236_G240_L0.9211.00
219_Y222_T0.9191.00
37_L234_I0.9101.00
136_F139_V0.9081.00
166_A181_I0.9051.00
96_V100_I0.9031.00
119_E125_R0.9031.00
137_R140_D0.9001.00
113_Y116_A0.8981.00
106_R111_G0.8971.00
224_T227_S0.8941.00
222_T225_G0.8911.00
64_A67_G0.8911.00
11_L18_D0.8851.00
162_V167_L0.8821.00
47_H55_A0.8761.00
262_L268_V0.8741.00
124_H127_L0.8721.00
180_A183_L0.8671.00
104_F107_M0.8601.00
263_G274_V0.8581.00
253_F258_A0.8561.00
46_G51_V0.8561.00
233_L236_G0.8441.00
228_V231_A0.8441.00
241_G245_D0.8441.00
267_Y274_V0.8421.00
178_F182_L0.8411.00
144_H147_P0.8351.00
266_G277_W0.8341.00
226_L230_V0.8341.00
77_V81_E0.8321.00
30_L36_P0.8291.00
121_L125_R0.8281.00
93_N97_L0.8241.00
129_A132_F0.8221.00
40_G47_H0.8221.00
230_V269_I0.8221.00
195_C205_A0.8221.00
7_L11_L0.8211.00
256_A260_L0.8211.00
68_L71_L0.8191.00
73_M77_V0.8191.00
170_G173_A0.8171.00
61_G64_A0.8161.00
63_L66_L0.8041.00
214_G218_Y0.8021.00
248_G252_G0.8021.00
115_E125_R0.8011.00
5_G216_K0.8011.00
240_L243_L0.8001.00
56_V182_L0.8001.00
226_L234_I0.7921.00
195_C198_A0.7881.00
169_A173_A0.7861.00
132_F135_L0.7830.99
276_T280_A0.7830.99
160_T163_H0.7820.99
114_D118_L0.7810.99
211_F216_K0.7790.99
47_H52_T0.7780.99
244_A248_G0.7710.99
274_V277_W0.7690.99
133_G136_F0.7680.99
151_L155_G0.7660.99
273_F277_W0.7620.99
136_F140_D0.7610.99
59_A66_L0.7580.99
202_L205_A0.7520.99
251_G254_W0.7460.99
92_G95_L0.7450.99
134_P138_L0.7410.99
189_A192_M0.7350.99
259_A262_L0.7350.99
124_H129_A0.7330.99
245_D248_G0.7270.99
229_T234_I0.7230.99
201_V205_A0.7200.99
186_L190_A0.7200.99
71_L74_V0.7190.99
240_L253_F0.7190.99
234_I238_E0.7190.99
34_R141_H0.7190.99
39_V145_M0.7140.99
147_P150_L0.7120.99
261_D264_L0.7110.99
112_R116_A0.7100.99
129_A134_P0.7000.99
214_G221_M0.6960.99
129_A136_F0.6950.99
254_W258_A0.6940.99
29_L39_V0.6920.99
262_L267_Y0.6900.99
202_L212_A0.6890.99
94_L232_L0.6860.99
167_L171_A0.6820.99
257_L260_L0.6820.99
70_A74_V0.6820.99
238_E241_G0.6810.99
62_A65_L0.6800.99
125_R129_A0.6770.99
134_P137_R0.6730.98
88_I95_L0.6710.98
114_D122_L0.6710.98
5_G9_Y0.6660.98
143_W147_P0.6630.98
100_I104_F0.6620.98
85_F89_I0.6600.98
159_A162_V0.6600.98
254_W259_A0.6600.98
242_L245_D0.6550.98
35_R40_G0.6540.98
46_G50_V0.6540.98
224_T228_V0.6540.98
44_S47_H0.6540.98
46_G49_T0.6510.98
83_A87_L0.6500.98
61_G66_L0.6500.98
155_G159_A0.6500.98
262_L273_F0.6460.98
263_G269_I0.6440.98
15_H21_H0.6440.98
276_T279_V0.6430.98
163_H166_A0.6430.98
216_K220_N0.6410.98
87_L96_V0.6400.98
48_S52_T0.6390.98
51_V59_A0.6390.98
256_A261_D0.6380.98
43_F187_L0.6340.98
269_I272_L0.6280.98
47_H54_L0.6270.98
18_D25_I0.6240.98
181_I185_P0.6230.97
269_I273_F0.6230.97
228_V233_L0.6230.97
183_L187_L0.6220.97
180_A186_L0.6210.97
37_L204_L0.6210.97
26_T30_L0.6200.97
68_L75_G0.6200.97
128_H131_L0.6190.97
195_C204_L0.6150.97
182_L186_L0.6150.97
75_G79_G0.6150.97
60_L64_A0.6120.97
27_R30_L0.6110.97
263_G270_V0.6080.97
132_F136_F0.6070.97
152_F156_F0.6060.97
52_T59_A0.6060.97
176_L179_W0.6050.97
55_A62_A0.6050.97
49_T53_L0.6040.97
66_L72_H0.6030.97
231_A234_I0.6010.97
105_R108_R0.6010.97
230_V234_I0.5980.97
128_H133_G0.5970.97
118_L123_A0.5920.97
68_L74_V0.5910.97
139_V143_W0.5900.97
114_D120_H0.5900.97
170_G175_G0.5890.96
80_T85_F0.5870.96
50_V54_L0.5860.96
30_L208_G0.5850.96
135_L139_V0.5850.96
58_L163_H0.5850.96
7_L10_T0.5840.96
112_R115_E0.5830.96
271_G274_V0.5820.96
122_L127_L0.5810.96
261_D265_L0.5810.96
225_G234_I0.5790.96
160_T164_L0.5780.96
114_D123_A0.5770.96
26_T218_Y0.5740.96
76_G80_T0.5730.96
44_S55_A0.5730.96
20_D24_A0.5720.96
72_H78_I0.5720.96
79_G82_V0.5720.96
244_A249_L0.5710.96
176_L182_L0.5680.96
146_Y151_L0.5660.96
115_E118_L0.5640.96
168_A173_A0.5640.96
71_L76_G0.5640.96
55_A58_L0.5630.95
74_V79_G0.5630.95
162_V165_L0.5610.95
198_A214_G0.5610.95
68_L72_H0.5590.95
86_L89_I0.5570.95
53_L56_V0.5560.95
32_A140_D0.5550.95
42_F46_G0.5520.95
179_W182_L0.5520.95
249_L252_G0.5510.95
58_L61_G0.5500.95
39_V44_S0.5490.95
119_E131_L0.5490.95
109_R114_D0.5430.95
218_Y221_M0.5410.94
245_D250_S0.5410.94
268_V275_L0.5410.94
55_A166_A0.5410.94
73_M76_G0.5400.94
115_E122_L0.5390.94
221_M226_L0.5360.94
206_A211_F0.5310.94
245_D249_L0.5310.94
252_G256_A0.5280.94
76_G79_G0.5250.94
127_L132_F0.5230.93
50_V53_L0.5230.93
231_A235_G0.5220.93
126_G134_P0.5220.93
265_L268_V0.5200.93
158_T162_V0.5200.93
253_F260_L0.5190.93
6_L9_Y0.5180.93
204_L208_G0.5180.93
228_V234_I0.5180.93
224_T234_I0.5170.93
79_G85_F0.5160.93
30_L33_G0.5160.93
130_R133_G0.5150.93
107_M217_L0.5120.93
161_E166_A0.5110.93
103_V107_M0.5100.93
40_G43_F0.5100.93
106_R109_R0.5090.92
50_V187_L0.5060.92
234_I237_I0.5060.92
19_P23_K0.5020.92
224_T233_L0.5020.92
87_L239_L0.5010.92
95_L99_G0.5000.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4pypA 1 0.3227 26.9 0.94 Contact Map
2xq2A 2 0.3369 17.5 0.945 Contact Map
3wdoA 1 0.5426 14.5 0.947 Contact Map
2l9uA 2 0.1418 14.4 0.947 Contact Map
2kluA 1 0.2447 12.8 0.948 Contact Map
4k0jA 3 0.3369 12.8 0.948 Contact Map
4tphA 2 0.3227 9.4 0.951 Contact Map
2l2tA 2 0.156 8.3 0.953 Contact Map
2f93B 2 0.1702 7.5 0.954 Contact Map
4tkrA 2 0.4681 6.8 0.955 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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