GREMLIN Database
Peptidase_U4 - Sporulation factor SpoIIGA
PFAM: PF03419 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 284 (277)
Sequences: 2512 (2213)
Seq/√Len: 133.0
META: 0.715

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
38_G76_A4.6371.00
153_T246_K4.4011.00
148_Y165_L4.1921.00
41_L75_I3.8611.00
149_E162_K3.6871.00
241_G262_L3.5641.00
42_G72_M3.5491.00
151_E248_E3.3061.00
69_S93_Y3.1341.00
151_E160_E3.0051.00
151_E158_S2.9571.00
246_K257_E2.8241.00
163_A278_A2.7191.00
231_S234_G2.6931.00
150_V181_I2.6671.00
247_V261_V2.5331.00
16_Y39_A2.4591.00
185_Y267_P2.4341.00
149_E160_E2.3941.00
181_I247_V2.3861.00
161_L187_A2.3531.00
229_Y237_G2.3131.00
241_G264_G2.3081.00
16_Y32_R2.2911.00
152_I244_P2.2621.00
38_G72_M2.1931.00
227_I241_G2.1841.00
153_T156_G2.1831.00
147_L162_K2.1811.00
12_F43_A2.1371.00
173_D262_L2.1281.00
25_L95_V2.0451.00
38_G75_I2.0281.00
244_P263_I2.0081.00
161_L183_V2.0011.00
153_T158_S1.9961.00
23_A30_V1.9931.00
172_R281_H1.9601.00
239_L264_G1.9161.00
26_L88_L1.9151.00
19_L35_L1.9081.00
182_I239_L1.8831.00
184_E277_Q1.8261.00
28_R84_R1.8051.00
183_V188_L1.7791.00
173_D225_R1.7661.00
97_F105_A1.7031.00
149_E250_E1.6951.00
151_E250_E1.6871.00
23_A77_F1.6701.00
229_Y233_G1.6491.00
164_L279_L1.6381.00
89_L93_Y1.6351.00
248_E255_K1.6111.00
72_M92_F1.6031.00
150_V278_A1.5841.00
206_D210_E1.5581.00
228_P238_L1.5541.00
154_L242_F1.5221.00
231_S237_G1.5111.00
152_I161_L1.5031.00
172_R283_E1.4951.00
173_D241_G1.4881.00
8_F43_A1.4841.00
161_L278_A1.4421.00
220_W223_R1.4371.00
26_L91_V1.4281.00
173_D176_T1.4241.00
12_F15_D1.4181.00
18_L92_F1.4151.00
70_L85_F1.4151.00
178_K260_K1.3861.00
152_I183_V1.3781.00
65_K92_F1.3761.00
150_V163_A1.3641.00
152_I247_V1.3621.00
65_K69_S1.3551.00
2_V7_L1.3481.00
16_Y35_L1.3281.00
12_F36_L1.3121.00
193_P196_L1.2921.00
186_E189_K1.2881.00
226_L238_L1.2781.00
184_E270_L1.2691.00
14_M18_L1.2521.00
28_R88_L1.2481.00
122_G127_Y1.2381.00
148_Y280_L1.2011.00
5_D10_L1.1900.99
22_T92_F1.1850.99
271_S276_Y1.1830.99
194_E198_E1.1790.99
85_F89_L1.1620.99
224_L242_F1.1440.99
233_G237_G1.1420.99
96_S100_G1.1360.99
6_V10_L1.1260.99
207_D210_E1.1250.99
205_E208_L1.1180.99
70_L89_L1.1180.99
171_L229_Y1.1130.99
45_Y68_V1.1060.99
185_Y197_R1.1000.99
242_F265_I1.0960.99
185_Y189_K1.0930.99
174_P228_P1.0790.99
42_G92_F1.0650.99
187_A277_Q1.0630.99
181_I278_A1.0620.99
22_T77_F1.0590.99
127_Y131_K1.0570.99
18_L95_V1.0560.99
174_P227_I1.0540.99
43_A53_P1.0370.99
148_Y164_L1.0350.99
178_K243_K1.0290.98
171_L182_I1.0250.98
172_R177_G1.0230.98
181_I261_V1.0160.98
116_W123_A1.0120.98
103_L107_H1.0100.98
65_K104_L1.0080.98
240_L265_I0.9990.98
5_D228_P0.9950.98
209_D212_L0.9900.98
20_L35_L0.9860.98
22_T91_V0.9770.98
269_K274_G0.9760.98
176_T225_R0.9760.98
19_L72_M0.9750.98
12_F16_Y0.9720.98
215_L218_S0.9690.98
124_A128_I0.9640.98
10_L119_L0.9540.97
32_R36_L0.9480.97
208_L212_L0.9460.97
93_Y97_F0.9410.97
102_A105_A0.9410.97
102_A126_A0.9380.97
98_L102_A0.9370.97
86_L90_L0.9340.97
105_A109_L0.9330.97
184_E269_K0.9280.97
206_D209_D0.9270.97
128_I132_L0.9200.97
69_S92_F0.9190.97
62_L116_W0.9140.97
11_N46_A0.9130.97
182_I232_V0.9080.97
22_T95_V0.9070.97
68_V72_M0.9050.97
127_Y130_I0.9050.97
81_S84_R0.9030.96
155_G223_R0.8980.96
15_D42_G0.8950.96
95_V99_L0.8950.96
111_L115_G0.8890.96
131_K135_R0.8850.96
208_L211_I0.8830.96
79_P85_F0.8790.96
243_K260_K0.8750.96
223_R245_D0.8750.96
118_F167_T0.8730.96
118_F123_A0.8730.96
83_K87_K0.8720.96
207_D211_I0.8690.96
65_K96_S0.8670.96
104_L108_Y0.8650.95
258_I261_V0.8650.95
84_R88_L0.8510.95
15_D72_M0.8460.95
69_S73_V0.8450.95
30_V76_A0.8410.95
34_R78_G0.8360.95
232_V266_S0.8350.95
74_L85_F0.8340.94
118_F122_G0.8330.94
10_L18_L0.8320.94
102_A106_L0.8240.94
12_F40_L0.8220.94
230_R235_E0.8180.94
77_F228_P0.8140.94
210_E213_E0.8140.94
183_V263_I0.8130.94
123_A127_Y0.8080.94
17_L123_A0.8050.93
23_A29_K0.8030.93
92_F96_S0.7960.93
176_T262_L0.7940.93
244_P278_A0.7930.93
96_S119_L0.7900.93
18_L96_S0.7820.92
229_Y234_G0.7820.92
228_P241_G0.7810.92
250_E255_K0.7790.92
250_E253_G0.7780.92
2_V6_V0.7750.92
175_L225_R0.7710.92
181_I244_P0.7690.92
47_V53_P0.7660.92
248_E257_E0.7640.91
65_K93_Y0.7620.91
229_Y232_V0.7620.91
180_V227_I0.7580.91
209_D213_E0.7500.91
77_F88_L0.7480.91
207_D213_E0.7480.91
67_L253_G0.7460.91
156_G245_D0.7450.90
94_L102_A0.7440.90
157_K191_L0.7440.90
163_A280_L0.7410.90
189_K197_R0.7400.90
73_V89_L0.7390.90
174_P241_G0.7380.90
247_V258_I0.7360.90
9_L13_L0.7360.90
185_Y192_L0.7350.90
153_T248_E0.7340.90
278_A281_H0.7320.90
118_F131_K0.7320.90
182_I276_Y0.7290.89
118_F127_Y0.7270.89
208_L213_E0.7250.89
242_F263_I0.7250.89
41_L71_L0.7250.89
19_L76_A0.7210.89
17_L127_Y0.7210.89
217_D221_A0.7170.89
270_L274_G0.7170.89
6_V9_L0.7170.89
143_K146_L0.7160.89
45_Y92_F0.7120.88
8_F47_V0.7100.88
243_K262_L0.7090.88
169_N280_L0.7080.88
239_L276_Y0.7080.88
8_F12_F0.7070.88
180_V232_V0.7040.88
150_V249_I0.7040.88
38_G42_G0.7040.88
232_V237_G0.7030.88
164_L276_Y0.7030.88
225_R243_K0.6950.87
154_L245_D0.6920.87
216_L219_E0.6920.87
45_Y65_K0.6900.87
2_V5_D0.6860.86
24_K28_R0.6850.86
233_G266_S0.6820.86
92_F105_A0.6810.86
148_Y249_I0.6810.86
16_Y20_L0.6810.86
186_E269_K0.6810.86
227_I262_L0.6780.86
134_F137_I0.6750.86
209_D216_L0.6750.86
165_L244_P0.6720.85
159_V190_E0.6720.85
205_E212_L0.6710.85
184_E229_Y0.6710.85
80_K84_R0.6700.85
178_K242_F0.6700.85
205_E210_E0.6700.85
2_V46_A0.6680.85
205_E213_E0.6600.84
234_G237_G0.6590.84
175_L262_L0.6580.84
117_L120_L0.6580.84
30_V35_L0.6580.84
17_L48_L0.6540.84
167_T170_Q0.6530.84
21_L95_V0.6530.84
206_D213_E0.6480.83
223_R243_K0.6480.83
39_A42_G0.6480.83
183_V278_A0.6470.83
78_G88_L0.6460.83
213_E216_L0.6450.83
65_K105_A0.6430.83
246_K259_K0.6430.83
73_V93_Y0.6400.82
215_L279_L0.6400.82
10_L96_S0.6390.82
169_N281_H0.6380.82
155_G219_E0.6380.82
162_K277_Q0.6340.82
178_K225_R0.6300.81
123_A130_I0.6270.81
130_I167_T0.6270.81
251_G254_E0.6270.81
124_A127_Y0.6260.81
19_L42_G0.6250.81
184_E274_G0.6250.81
185_Y200_L0.6220.81
23_A76_A0.6220.81
156_G273_D0.6210.81
175_L226_L0.6210.81
18_L99_L0.6200.80
205_E209_D0.6190.80
156_G159_V0.6170.80
56_S59_L0.6160.80
271_S274_G0.6150.80
116_W119_L0.6130.80
240_L244_P0.6120.80
121_L124_A0.6100.79
90_L94_L0.6100.79
15_D43_A0.6090.79
66_L73_V0.6070.79
171_L237_G0.6040.79
159_V191_L0.6020.78
147_L164_L0.6000.78
15_D93_Y0.6000.78
119_L122_G0.6000.78
50_L60_Y0.6000.78
195_E217_D0.5960.78
61_S64_L0.5950.78
10_L14_M0.5950.78
179_P280_L0.5950.78
79_P88_L0.5950.78
46_A72_M0.5930.77
211_I214_E0.5920.77
116_W127_Y0.5910.77
126_A129_L0.5890.77
99_L126_A0.5880.77
179_P261_V0.5860.77
261_V264_G0.5860.77
16_Y121_L0.5830.76
100_G133_V0.5830.76
214_E217_D0.5830.76
8_F15_D0.5810.76
195_E198_E0.5800.76
19_L38_G0.5790.76
209_D214_E0.5770.75
226_L240_L0.5730.75
206_D212_L0.5720.75
152_I263_I0.5710.75
39_A92_F0.5710.75
197_R215_L0.5700.75
45_Y59_L0.5700.75
12_F39_A0.5700.75
152_I278_A0.5690.74
232_V239_L0.5680.74
42_G45_Y0.5680.74
108_Y152_I0.5660.74
32_R82_L0.5650.74
211_I216_L0.5640.74
94_L255_K0.5630.74
210_E214_E0.5630.74
244_P247_V0.5610.73
165_L279_L0.5610.73
65_K72_M0.5590.73
84_R87_K0.5570.73
11_N14_M0.5550.73
256_I259_K0.5540.73
50_L53_P0.5540.73
45_Y100_G0.5530.72
172_R179_P0.5520.72
154_L192_L0.5520.72
28_R32_R0.5510.72
188_L265_I0.5500.72
124_A130_I0.5490.72
116_W128_I0.5460.71
129_L133_V0.5460.71
235_E268_G0.5460.71
154_L244_P0.5440.71
186_E277_Q0.5440.71
150_V161_L0.5440.71
51_F60_Y0.5410.71
180_V264_G0.5400.71
11_N15_D0.5400.71
20_L133_V0.5380.70
244_P264_G0.5380.70
4_L60_Y0.5360.70
59_L63_L0.5350.70
161_L244_P0.5340.70
65_K108_Y0.5330.70
191_L245_D0.5330.70
122_G126_A0.5330.70
7_L51_F0.5330.70
19_L73_V0.5320.69
29_K33_W0.5310.69
74_L79_P0.5310.69
46_A65_K0.5300.69
90_L147_L0.5300.69
13_L175_L0.5280.69
135_R138_K0.5280.69
161_L277_Q0.5270.69
231_S268_G0.5270.69
250_E256_I0.5270.69
150_V263_I0.5270.69
117_L121_L0.5260.69
186_E238_L0.5260.69
7_L100_G0.5240.68
146_L165_L0.5230.68
99_L103_L0.5210.68
69_S97_F0.5190.68
141_K144_K0.5190.68
42_G46_A0.5180.67
141_K167_T0.5180.67
249_I258_I0.5170.67
118_F129_L0.5140.67
68_V106_L0.5140.67
148_Y250_E0.5120.67
31_K34_R0.5110.66
4_L52_L0.5100.66
134_F138_K0.5090.66
241_G266_S0.5090.66
208_L280_L0.5080.66
206_D217_D0.5080.66
126_A130_I0.5060.66
99_L127_Y0.5040.65
244_P261_V0.5010.65
95_V167_T0.5010.65
181_I281_H0.5010.65
215_L221_A0.5000.65
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4xnjA 1 0.4683 14.3 0.956 Contact Map
2i1aA 2 0.3063 11.5 0.958 Contact Map
3wdoA 1 0.6937 10.8 0.958 Contact Map
3s8iA 2 0.331 9.4 0.959 Contact Map
3o7qA 1 0.4754 8.5 0.96 Contact Map
2hs1A 2 0.3134 8.1 0.96 Contact Map
4u4vA 1 0.4859 7.2 0.961 Contact Map
4ezcA 3 0.5282 5.9 0.963 Contact Map
2hahA 2 0.3521 5.5 0.963 Contact Map
2r9rB 3 0.4718 5.5 0.963 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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