GREMLIN Database
DUF222 - Domain of unknown function (DUF222)
PFAM: PF02720 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 301 (294)
Sequences: 14802 (11404)
Seq/√Len: 665.1
META: 0.781

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
162_V173_G5.0861.00
172_D208_V3.6591.00
12_A16_A3.6311.00
12_A19_A3.4111.00
290_P293_L3.2051.00
150_R205_D3.0351.00
169_P172_D2.8601.00
80_E117_R2.7991.00
199_P203_R2.6961.00
235_D238_T2.5661.00
151_R200_A2.4581.00
120_T123_Q2.3961.00
168_L204_A2.3421.00
157_D163_R2.3351.00
163_R167_R2.3301.00
198_T201_Q2.3001.00
153_T165_R2.2151.00
256_P259_T2.2131.00
154_L177_D2.1751.00
175_A212_R2.1521.00
152_L164_L2.1331.00
294_R298_R2.0551.00
9_E19_A2.0501.00
161_M167_R1.9891.00
61_V87_R1.9851.00
119_L127_A1.9811.00
76_L86_A1.9771.00
166_G207_L1.9411.00
72_T89_I1.9351.00
143_D147_A1.9091.00
157_D167_R1.8761.00
16_A19_A1.8531.00
176_L211_A1.8421.00
282_V290_P1.7981.00
235_D239_L1.7951.00
136_D139_G1.7811.00
168_L173_G1.7501.00
152_L166_G1.7121.00
106_D109_E1.7041.00
82_S85_H1.7041.00
9_E13_R1.6871.00
11_A15_L1.6701.00
115_R119_L1.6641.00
57_R87_R1.6591.00
162_V177_D1.6541.00
45_A52_P1.6161.00
210_L214_A1.6151.00
74_A78_A1.5851.00
101_D104_A1.5791.00
177_D181_D1.5631.00
130_R134_R1.5521.00
109_E113_L1.5501.00
277_G281_D1.5441.00
236_L240_A1.5431.00
109_E112_L1.5381.00
207_L211_A1.5381.00
238_T241_G1.5191.00
155_R165_R1.5181.00
246_A249_L1.5001.00
144_E148_R1.4971.00
115_R127_A1.4961.00
41_A56_R1.4951.00
127_A130_R1.4641.00
255_I260_A1.4611.00
61_V83_Y1.4321.00
162_V170_P1.4241.00
268_S285_T1.4131.00
247_A250_G1.4011.00
153_T200_A1.4001.00
181_D203_R1.3971.00
72_T86_A1.3971.00
183_L186_P1.3941.00
116_A124_L1.3731.00
50_L55_A1.3731.00
72_T81_I1.3561.00
54_A84_A1.3361.00
257_A261_R1.3301.00
75_A80_E1.3251.00
61_V86_A1.3211.00
291_P295_R1.3191.00
271_V299_A1.3161.00
12_A15_L1.3151.00
258_E261_R1.3111.00
85_H124_L1.2961.00
168_L176_L1.2911.00
27_D40_A1.2911.00
30_G33_A1.2831.00
156_P162_V1.2821.00
176_L207_L1.2751.00
144_E147_A1.2671.00
20_A24_A1.2651.00
255_I259_T1.2451.00
289_P293_L1.2391.00
18_A22_L1.2351.00
65_L73_A1.2161.00
154_L162_V1.2111.00
72_T109_E1.2101.00
197_R205_D1.2041.00
11_A14_R1.1981.00
184_A203_R1.1971.00
18_A21_R1.1921.00
152_L180_L1.1891.00
282_V286_T1.1781.00
175_A208_V1.1701.00
25_E29_R1.1661.00
156_P177_D1.1661.00
119_L123_Q1.1601.00
84_A87_R1.1601.00
71_A110_A1.1531.00
201_Q205_D1.1491.00
32_W40_A1.1481.00
89_I124_L1.1411.00
122_A126_R1.1381.00
151_R167_R1.1271.00
19_A23_L1.1261.00
73_A77_A1.1191.00
112_L116_A1.1171.00
289_P297_L1.1131.00
88_A125_R1.0981.00
234_V239_L1.0931.00
143_D146_A1.0931.00
102_A105_V1.0931.00
274_D278_E1.0651.00
71_A74_A1.0621.00
108_A112_L1.0601.00
52_P56_R1.0551.00
142_R146_A1.0471.00
20_A23_L1.0401.00
15_L19_A1.0341.00
72_T113_L1.0331.00
54_A58_R1.0271.00
37_A46_A1.0211.00
209_E213_R1.0191.00
9_E12_A1.0181.00
183_L210_L1.0171.00
66_R73_A1.0141.00
174_A178_A1.0071.00
171_E262_R1.0061.00
286_T290_P1.0021.00
275_P281_D1.0021.00
41_A52_P1.0001.00
211_A215_L0.9981.00
178_A182_A0.9981.00
14_R18_A0.9971.00
50_L58_R0.9961.00
166_G173_G0.9871.00
105_V109_E0.9871.00
69_L90_A0.9861.00
164_L176_L0.9851.00
154_L173_G0.9811.00
22_L26_L0.9781.00
51_S54_A0.9751.00
111_A115_R0.9731.00
27_D32_W0.9701.00
282_V293_L0.9691.00
151_R165_R0.9681.00
124_L128_A0.9651.00
49_G85_H0.9621.00
115_R118_R0.9571.00
271_V282_V0.9571.00
169_P208_V0.9551.00
228_P233_T0.9531.00
64_A90_A0.9531.00
110_A114_E0.9531.00
76_L83_Y0.9521.00
234_V238_T0.9491.00
85_H121_P0.9431.00
278_E292_A0.9431.00
19_A26_L0.9431.00
130_R133_A0.9411.00
286_T289_P0.9411.00
27_D63_R0.9401.00
8_L18_A0.9381.00
13_R19_A0.9361.00
56_R60_R0.9341.00
171_E259_T0.9331.00
179_A183_L0.9331.00
235_D241_G0.9291.00
238_T242_G0.9281.00
92_A128_A0.9271.00
143_D148_R0.9261.00
212_R216_A0.9251.00
191_A194_G0.9251.00
145_R148_R0.9231.00
259_T262_R0.9221.00
181_D185_A0.9201.00
188_P202_R0.9181.00
9_E16_A0.9151.00
293_L297_L0.9141.00
125_R129_R0.9101.00
202_R206_A0.9081.00
8_L19_A0.9051.00
91_R125_R0.9021.00
279_P282_V0.8991.00
8_L15_L0.8951.00
278_E281_D0.8951.00
250_G253_G0.8931.00
13_R16_A0.8921.00
75_A81_I0.8891.00
16_A20_A0.8861.00
39_S42_A0.8851.00
176_L208_V0.8851.00
270_R295_R0.8811.00
235_D240_A0.8811.00
237_E241_G0.8771.00
15_L18_A0.8771.00
137_P140_A0.8761.00
8_L22_L0.8751.00
155_R163_R0.8751.00
101_D105_V0.8721.00
197_R201_Q0.8691.00
209_E212_R0.8661.00
24_A66_R0.8641.00
246_A260_A0.8621.00
57_R61_V0.8591.00
21_R77_A0.8521.00
21_R73_A0.8491.00
57_R84_A0.8481.00
53_G57_R0.8471.00
74_A77_A0.8451.00
32_W36_G0.8451.00
21_R24_A0.8441.00
11_A18_A0.8431.00
183_L187_L0.8421.00
42_A52_P0.8401.00
41_A45_A0.8381.00
104_A107_A0.8351.00
246_A250_G0.8351.00
64_A94_A0.8321.00
179_A210_L0.8321.00
180_L203_R0.8301.00
114_E118_R0.8291.00
247_A251_G0.8281.00
23_L26_L0.8271.00
187_L190_D0.8261.00
10_A14_R0.8251.00
185_A188_P0.8221.00
270_R273_L0.8201.00
119_L124_L0.8181.00
50_L54_A0.8181.00
236_L241_G0.8121.00
147_A150_R0.8061.00
213_R217_A0.8021.00
29_R32_W0.7991.00
41_A55_A0.7971.00
28_A66_R0.7961.00
21_R25_E0.7941.00
71_A109_E0.7931.00
233_T239_L0.7921.00
241_G244_G0.7901.00
109_E116_A0.7901.00
98_D101_D0.7901.00
103_A106_D0.7881.00
183_L209_E0.7871.00
128_A132_R0.7861.00
229_Q233_T0.7851.00
14_R19_A0.7841.00
83_Y87_R0.7801.00
105_V108_A0.7801.00
108_A111_A0.7791.00
8_L23_L0.7731.00
103_A107_A0.7731.00
246_A251_G0.7671.00
54_A57_R0.7631.00
114_E117_R0.7611.00
185_A189_A0.7601.00
157_D161_M0.7601.00
23_L44_L0.7591.00
181_D184_A0.7581.00
113_L116_A0.7571.00
106_D110_A0.7551.00
19_A22_L0.7551.00
19_A58_R0.7491.00
237_E240_A0.7421.00
71_A113_L0.7411.00
102_A106_D0.7371.00
164_L168_L0.7341.00
13_R26_L0.7321.00
95_A132_R0.7301.00
123_Q126_R0.7241.00
13_R18_A0.7221.00
88_A124_L0.7221.00
233_T237_E0.7211.00
59_V63_R0.7181.00
87_R91_R0.7161.00
156_P174_A0.7131.00
112_L128_A0.7121.00
131_L134_R0.7111.00
154_L166_G0.7081.00
10_A13_R0.7051.00
179_A215_L0.7031.00
143_D256_P0.7031.00
32_W37_A0.7021.00
185_A199_P0.7001.00
277_G280_L0.6941.00
128_A131_L0.6931.00
228_P232_V0.6901.00
88_A128_A0.6901.00
69_L72_T0.6871.00
280_L283_G0.6831.00
145_R149_R0.6811.00
154_L164_L0.6811.00
13_R21_R0.6811.00
204_A208_V0.6781.00
249_L252_G0.6771.00
69_L93_A0.6761.00
230_V234_V0.6761.00
65_L86_A0.6761.00
238_T244_G0.6741.00
88_A91_R0.6741.00
234_V260_A0.6721.00
226_V229_Q0.6721.00
271_V275_P0.6701.00
194_G197_R0.6681.00
26_L31_G0.6671.00
261_R265_C0.6661.00
32_W35_D0.6641.00
206_A210_L0.6631.00
61_V90_A0.6601.00
150_R201_Q0.6591.00
183_L214_A0.6591.00
136_D140_A0.6591.00
14_R21_R0.6581.00
36_G39_S0.6581.00
12_A23_L0.6581.00
191_A195_D0.6551.00
242_G245_A0.6541.00
17_A76_L0.6521.00
133_A137_P0.6501.00
198_T205_D0.6481.00
113_L117_R0.6461.00
179_A182_A0.6461.00
179_A211_A0.6441.00
247_A260_A0.6441.00
16_A23_L0.6431.00
230_V239_L0.6431.00
248_L251_G0.6411.00
211_A214_A0.6401.00
142_R145_R0.6371.00
27_D31_G0.6371.00
53_G56_R0.6361.00
43_W47_A0.6351.00
108_A131_L0.6351.00
159_D167_R0.6311.00
289_P294_R0.6251.00
233_T241_G0.6251.00
131_L135_L0.6241.00
271_V274_D0.6241.00
17_A21_R0.6231.00
180_L184_A0.6231.00
280_L296_A0.6211.00
150_R169_P0.6211.00
31_G34_A0.6211.00
150_R161_M0.6141.00
281_D284_R0.6121.00
64_A68_R0.6111.00
175_A211_A0.6091.00
149_R167_R0.6081.00
168_L208_V0.6061.00
127_A131_L0.6061.00
168_L172_D0.6041.00
229_Q270_R0.6041.00
287_R297_L0.5991.00
272_V275_P0.5971.00
187_L202_R0.5961.00
238_T245_A0.5951.00
282_V295_R0.5941.00
208_V211_A0.5941.00
262_R265_C0.5931.00
239_L242_G0.5911.00
67_E70_P0.5891.00
42_A46_A0.5891.00
279_P296_A0.5871.00
93_A96_L0.5861.00
107_A111_A0.5851.00
12_A20_A0.5851.00
163_R166_G0.5831.00
230_V264_A0.5831.00
257_A260_A0.5821.00
112_L115_R0.5811.00
142_R147_A0.5801.00
11_A19_A0.5791.00
97_P100_V0.5761.00
97_P101_D0.5751.00
38_R42_A0.5731.00
220_L224_G0.5701.00
8_L11_A0.5691.00
17_A20_A0.5661.00
229_Q268_S0.5651.00
12_A18_A0.5631.00
57_R83_Y0.5631.00
235_D243_A0.5621.00
239_L243_A0.5621.00
223_P226_V0.5591.00
75_A113_L0.5591.00
146_A170_P0.5581.00
174_A177_D0.5571.00
219_A223_P0.5561.00
16_A83_Y0.5551.00
214_A217_A0.5531.00
81_I85_H0.5531.00
16_A22_L0.5521.00
40_A59_V0.5511.00
172_D211_A0.5471.00
237_E242_G0.5451.00
9_E23_L0.5451.00
231_V271_V0.5451.00
261_R264_A0.5451.00
149_R161_M0.5441.00
180_L207_L0.5441.00
183_L213_R0.5421.00
203_R210_L0.5401.00
60_R64_A0.5391.00
233_T236_L0.5391.00
8_L14_R0.5381.00
280_L293_L0.5381.00
129_R133_A0.5371.00
58_R62_A0.5361.00
65_L76_L0.5361.00
86_A90_A0.5361.00
23_L58_R0.5351.00
20_A25_E0.5321.00
166_G176_L0.5321.00
24_A28_A0.5270.99
92_A96_L0.5270.99
200_A203_R0.5260.99
14_R20_A0.5210.99
240_A243_A0.5200.99
11_A16_A0.5140.99
16_A26_L0.5120.99
138_D141_E0.5110.99
20_A26_L0.5110.99
111_A127_A0.5100.99
191_A196_P0.5100.99
88_A92_A0.5090.99
241_G245_A0.5080.99
10_A19_A0.5060.99
129_R132_R0.5040.99
161_M169_P0.5020.99
236_L242_G0.5010.99
279_P283_G0.5000.99
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1vz0A 3 0.3621 72.4 0.919 Contact Map
3mkyB 2 0.3754 55.6 0.927 Contact Map
4umkA 2 0.3721 54.9 0.928 Contact Map
1r71A 2 0.3688 7.4 0.953 Contact Map
4nnoA 1 0.1694 2.7 0.962 Contact Map
1p4wA 1 0.2525 2.5 0.963 Contact Map
3sztA 2 0.2658 1.7 0.966 Contact Map
2rnjA 1 0.2126 1.6 0.967 Contact Map
4nqwB 1 0.2226 1.5 0.967 Contact Map
2q0oA 2 0.2625 1.4 0.967 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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