FlgI - PFAM28P - GREMLIN Contacts

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FlgI - Flagellar P-ring protein

PFAM:	PF02119	Sequences: RIKDIASVEGVRDNQLVGYGLVVGLNGTGDSTRQTPFTVQSLANMLERLGVTVPPGDLKLKNVAAVMVTATLPPFARPGQRIDVTVSSIGDAKSLRGGTLLMTPLKGADGQVYAVAQGPLSVGGFGASGGGGSSVTKNHPTVGRIPNGAIVEREVPFDFAQGGSLRLNLRNPDFTTASRIADAINAAFGPGTARALDAGTVRVRVPADYPGQRVAFLARIENLEVEPDAPARVVINERTGTIVMGGDVRISPVAVAHGNLTVTITETPQVSQPNPFSGQTVVVPRTDIEVEEEKGRLFVLPEGATLQDLVRALNALGATPRDLIAILQALKAAGALQAELEV	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_10
Length:	342 (341)
Sequences:	8543 (4980)
Seq/√Len:	269.7
META:	0.833

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
68_V	103_T	3.951	1.00
114_A	149_A	3.839	1.00
2_I	180_I	3.433	1.00
13_D	71_T	3.360	1.00
183_A	223_L	2.956	1.00
78_P	154_E	2.847	1.00
270_V	283_V	2.827	1.00
164_S	202_R	2.809	1.00
253_V	330_L	2.808	1.00
61_K	93_K	2.730	1.00
77_R	197_D	2.686	1.00
187_A	219_R	2.672	1.00
6_A	180_I	2.638	1.00
7_S	170_R	2.632	1.00
274_N	277_S	2.556	1.00
212_Q	215_A	2.545	1.00
175_T	179_R	2.493	1.00
12_R	152_E	2.424	1.00
269_Q	283_V	2.401	1.00
232_R	341_E	2.385	1.00
268_P	285_R	2.379	1.00
188_F	216_F	2.362	1.00
185_N	193_A	2.351	1.00
203_V	220_I	2.265	1.00
19_Y	41_S	2.209	1.00
196_L	202_R	2.206	1.00
206_P	216_F	2.197	1.00
3_K	173_D	2.183	1.00
180_I	201_V	2.180	1.00
251_S	337_Q	2.163	1.00
175_T	221_E	2.135	1.00
120_L	143_G	2.109	1.00
84_V	116_A	2.078	1.00
269_Q	285_R	2.072	1.00
181_A	195_A	2.069	1.00
268_P	284_P	2.055	1.00
179_R	227_P	2.028	1.00
197_D	200_T	2.013	1.00
271_S	280_T	2.010	1.00
266_E	286_T	2.004	1.00
233_V	336_L	1.995	1.00
6_A	167_L	1.984	1.00
310_V	327_L	1.973	1.00
194_R	202_R	1.901	1.00
195_A	214_V	1.881	1.00
62_N	88_S	1.859	1.00
188_F	192_T	1.852	1.00
85_T	142_V	1.833	1.00
72_L	151_V	1.824	1.00
175_T	218_A	1.822	1.00
172_P	199_G	1.817	1.00
272_Q	280_T	1.815	1.00
178_S	182_D	1.808	1.00
169_L	177_A	1.791	1.00
137_K	292_E	1.770	1.00
31_S	91_D	1.765	1.00
131_G	286_T	1.740	1.00
307_Q	311_R	1.739	1.00
14_N	152_E	1.731	1.00
5_I	225_V	1.703	1.00
76_A	151_V	1.688	1.00
54_P	57_D	1.688	1.00
3_K	228_D	1.683	1.00
84_V	149_A	1.681	1.00
17_V	101_L	1.678	1.00
175_T	222_N	1.665	1.00
131_G	266_E	1.643	1.00
71_T	81_R	1.628	1.00
8_V	217_L	1.625	1.00
108_A	115_V	1.622	1.00
268_P	282_V	1.618	1.00
9_E	166_R	1.603	1.00
31_S	59_K	1.583	1.00
67_M	101_L	1.578	1.00
180_I	225_V	1.577	1.00
166_R	202_R	1.574	1.00
174_F	217_L	1.565	1.00
298_F	316_L	1.545	1.00
35_T	60_L	1.541	1.00
6_A	221_E	1.532	1.00
130_G	286_T	1.520	1.00
266_E	284_P	1.514	1.00
166_R	200_T	1.508	1.00
205_V	213_R	1.500	1.00
70_A	82_I	1.484	1.00
26_N	140_P	1.477	1.00
2_I	220_I	1.463	1.00
77_R	200_T	1.463	1.00
130_G	133_S	1.447	1.00
102_M	108_A	1.447	1.00
1_R	4_D	1.436	1.00
107_G	113_Y	1.426	1.00
42_L	60_L	1.415	1.00
8_V	167_L	1.395	1.00
72_L	155_V	1.394	1.00
11_V	174_F	1.390	1.00
94_S	122_V	1.390	1.00
87_S	99_T	1.364	1.00
37_F	60_L	1.362	1.00
250_I	335_A	1.353	1.00
184_I	223_L	1.350	1.00
31_S	34_Q	1.331	1.00
236_N	239_T	1.329	1.00
277_S	280_T	1.326	1.00
177_A	195_A	1.311	1.00
136_T	140_P	1.306	1.00
109_D	113_Y	1.300	1.00
297_L	313_L	1.290	1.00
306_L	310_V	1.282	1.00
244_M	310_V	1.277	1.00
125_F	137_K	1.256	1.00
271_S	281_V	1.249	1.00
273_P	280_T	1.248	1.00
177_A	181_A	1.247	1.00
179_R	228_D	1.243	1.00
19_Y	65_A	1.240	1.00
2_I	223_L	1.234	1.00
188_F	219_R	1.226	1.00
189_G	192_T	1.201	1.00
182_D	186_A	1.200	1.00
41_S	65_A	1.200	1.00
102_M	115_V	1.199	1.00
230_P	339_E	1.192	1.00
292_E	321_R	1.182	1.00
218_A	222_N	1.181	1.00
121_S	342_V	1.173	1.00
7_S	80_Q	1.169	1.00
259_N	293_E	1.156	1.00
178_S	215_A	1.125	1.00
206_P	209_Y	1.123	1.00
133_S	286_T	1.120	1.00
80_Q	168_N	1.113	1.00
13_D	81_R	1.111	1.00
77_R	154_E	1.103	1.00
13_D	150_I	1.098	1.00
271_S	283_V	1.090	1.00
176_T	227_P	1.087	1.00
272_Q	278_G	1.083	1.00
21_L	63_V	1.083	1.00
165_L	217_L	1.080	1.00
135_V	290_V	1.080	1.00
99_T	102_M	1.076	1.00
125_F	290_V	1.071	1.00
158_D	161_Q	1.062	1.00
237_E	321_R	1.059	1.00
267_T	285_R	1.051	1.00
5_I	180_I	1.043	1.00
262_V	309_L	1.042	1.00
5_I	227_P	1.039	1.00
256_A	297_L	1.039	1.00
73_P	76_A	1.038	1.00
159_F	174_F	1.037	1.00
297_L	300_L	1.036	1.00
1_R	228_D	1.033	1.00
111_Q	153_R	1.030	1.00
265_T	285_R	1.030	1.00
139_H	142_V	1.029	1.00
235_I	324_I	1.013	1.00
172_P	198_A	1.005	1.00
3_K	221_E	1.005	1.00
42_L	49_L	1.003	1.00
126_G	140_P	1.003	1.00
60_L	63_V	0.992	1.00
267_T	287_D	0.990	1.00
176_T	225_V	0.985	1.00
271_S	277_S	0.982	1.00
167_L	203_V	0.980	1.00
8_V	165_L	0.978	1.00
133_S	266_E	0.968	1.00
297_L	323_L	0.967	1.00
180_I	223_L	0.955	1.00
121_S	237_E	0.954	1.00
2_I	6_A	0.954	1.00
180_I	184_I	0.952	1.00
181_A	193_A	0.951	1.00
99_T	142_V	0.951	1.00
176_T	228_D	0.950	1.00
331_K	339_E	0.949	1.00
115_V	150_I	0.949	1.00
15_Q	71_T	0.947	1.00
159_F	217_L	0.946	1.00
77_R	199_G	0.943	1.00
21_L	45_M	0.942	1.00
27_G	61_K	0.941	1.00
245_G	305_T	0.938	1.00
208_D	212_Q	0.935	1.00
308_D	311_R	0.930	1.00
331_K	338_A	0.918	1.00
232_R	339_E	0.913	1.00
209_Y	212_Q	0.913	1.00
192_T	216_F	0.910	1.00
33_R	59_K	0.910	1.00
182_D	215_A	0.908	1.00
44_N	51_V	0.907	1.00
235_I	327_L	0.907	1.00
69_T	99_T	0.906	1.00
34_Q	59_K	0.904	1.00
270_V	273_P	0.903	1.00
255_V	262_V	0.903	1.00
233_V	330_L	0.902	1.00
48_R	106_K	0.899	1.00
252_P	299_V	0.897	1.00
42_L	89_I	0.897	1.00
177_A	201_V	0.896	1.00
252_P	302_E	0.895	1.00
44_N	91_D	0.893	1.00
318_A	326_I	0.890	1.00
11_V	75_F	0.890	1.00
96_R	237_E	0.887	1.00
59_K	91_D	0.887	1.00
336_L	340_L	0.886	1.00
268_P	283_V	0.877	1.00
244_M	327_L	0.876	1.00
68_V	84_V	0.874	1.00
246_G	252_P	0.866	1.00
165_L	203_V	0.863	1.00
96_R	321_R	0.861	1.00
318_A	323_L	0.861	1.00
34_Q	91_D	0.861	1.00
184_I	203_V	0.859	1.00
168_N	199_G	0.856	1.00
235_I	340_L	0.855	1.00
4_D	231_A	0.850	1.00
144_R	332_A	0.850	1.00
2_I	225_V	0.849	1.00
185_N	192_T	0.848	1.00
174_F	198_A	0.848	1.00
179_R	182_D	0.848	1.00
178_S	214_V	0.848	1.00
73_P	82_I	0.848	1.00
250_I	309_L	0.847	1.00
82_I	151_V	0.846	1.00
183_A	186_A	0.845	1.00
33_R	61_K	0.844	1.00
15_Q	150_I	0.836	0.99
327_L	330_L	0.836	0.99
128_S	288_I	0.834	0.99
81_R	150_I	0.832	0.99
252_P	301_P	0.832	0.99
168_N	200_T	0.831	0.99
187_A	223_L	0.829	0.99
38_T	41_S	0.828	0.99
15_Q	102_M	0.828	0.99
325_A	328_Q	0.826	0.99
126_G	134_S	0.825	0.99
39_V	60_L	0.823	0.99
209_Y	219_R	0.818	0.99
31_S	61_K	0.817	0.99
31_S	93_K	0.810	0.99
39_V	42_L	0.809	0.99
30_D	64_A	0.807	0.99
123_G	237_E	0.806	0.99
38_T	63_V	0.804	0.99
65_A	117_Q	0.802	0.99
30_D	90_G	0.801	0.99
19_Y	38_T	0.800	0.99
43_A	55_P	0.800	0.99
144_R	328_Q	0.799	0.99
42_L	45_M	0.798	0.99
132_G	285_R	0.794	0.99
81_R	147_N	0.792	0.99
17_V	49_L	0.788	0.99
265_T	287_D	0.787	0.99
77_R	168_N	0.782	0.99
11_V	14_N	0.781	0.99
173_D	221_E	0.781	0.99
125_F	138_N	0.781	0.99
241_T	245_G	0.779	0.99
306_L	327_L	0.773	0.99
270_V	280_T	0.773	0.99
233_V	340_L	0.773	0.99
245_G	306_L	0.772	0.99
273_P	278_G	0.769	0.99
16_L	72_L	0.769	0.99
330_L	333_A	0.768	0.99
297_L	309_L	0.768	0.99
163_G	204_R	0.767	0.99
276_F	280_T	0.759	0.99
185_N	191_G	0.753	0.99
37_F	65_A	0.753	0.99
32_T	60_L	0.747	0.99
194_R	204_R	0.746	0.99
254_A	297_L	0.741	0.99
281_V	284_P	0.741	0.99
266_E	285_R	0.738	0.99
246_G	307_Q	0.737	0.99
273_P	277_S	0.737	0.99
63_V	89_I	0.734	0.99
259_N	322_D	0.733	0.99
256_A	320_P	0.730	0.99
325_A	329_A	0.729	0.99
64_A	90_G	0.724	0.99
142_V	238_R	0.723	0.99
31_S	54_P	0.722	0.99
131_G	284_P	0.722	0.99
125_F	245_G	0.721	0.99
128_S	134_S	0.718	0.98
171_N	337_Q	0.715	0.98
272_Q	276_F	0.714	0.98
96_R	122_V	0.714	0.98
309_L	318_A	0.713	0.98
327_L	340_L	0.712	0.98
88_S	92_A	0.711	0.98
86_V	142_V	0.710	0.98
275_P	278_G	0.709	0.98
271_S	278_G	0.707	0.98
24_G	29_G	0.703	0.98
173_D	176_T	0.700	0.98
253_V	335_A	0.695	0.98
126_G	136_T	0.695	0.98
43_A	53_V	0.693	0.98
45_M	67_M	0.690	0.98
254_A	310_V	0.690	0.98
272_Q	277_S	0.689	0.98
20_G	24_G	0.688	0.98
207_A	210_P	0.685	0.98
135_V	263_T	0.683	0.98
282_V	285_R	0.682	0.98
330_L	336_L	0.681	0.98
96_R	137_K	0.681	0.98
133_S	288_I	0.678	0.98
106_K	112_V	0.677	0.98
295_G	319_T	0.676	0.98
71_T	74_P	0.675	0.98
41_S	45_M	0.673	0.98
205_V	209_Y	0.672	0.98
8_V	203_V	0.669	0.98
123_G	239_T	0.669	0.98
45_M	49_L	0.668	0.98
177_A	214_V	0.668	0.98
43_A	58_L	0.667	0.98
178_S	218_A	0.667	0.98
170_R	339_E	0.666	0.98
25_L	29_G	0.665	0.97
72_L	75_F	0.663	0.97
254_A	306_L	0.662	0.97
303_G	309_L	0.662	0.97
132_G	286_T	0.661	0.97
263_T	289_E	0.661	0.97
334_G	338_A	0.658	0.97
31_S	36_P	0.658	0.97
276_F	279_Q	0.658	0.97
222_N	228_D	0.657	0.97
174_F	214_V	0.657	0.97
125_F	321_R	0.655	0.97
311_R	315_A	0.654	0.97
178_S	219_R	0.651	0.97
72_L	82_I	0.651	0.97
31_S	35_T	0.650	0.97
60_L	91_D	0.647	0.97
320_P	323_L	0.646	0.97
85_T	119_P	0.646	0.97
244_M	248_V	0.646	0.97
204_R	207_A	0.642	0.97
187_A	190_P	0.642	0.97
270_V	282_V	0.642	0.97
122_V	143_G	0.642	0.97
37_F	63_V	0.641	0.97
270_V	281_V	0.641	0.97
164_S	204_R	0.641	0.97
86_V	145_I	0.639	0.97
15_Q	115_V	0.638	0.97
205_V	216_F	0.638	0.97
216_F	220_I	0.638	0.97
122_V	125_F	0.635	0.97
96_R	124_G	0.634	0.97
121_S	139_H	0.633	0.97
304_A	308_D	0.632	0.97
159_F	198_A	0.632	0.97
127_A	288_I	0.630	0.97
32_T	59_K	0.630	0.97
191_G	204_R	0.629	0.96
271_S	279_Q	0.629	0.96
166_R	196_L	0.627	0.96
12_R	15_Q	0.627	0.96
106_K	111_Q	0.627	0.96
12_R	107_G	0.626	0.96
37_F	51_V	0.623	0.96
53_V	58_L	0.623	0.96
67_M	89_I	0.623	0.96
309_L	313_L	0.622	0.96
169_L	180_I	0.619	0.96
2_I	167_L	0.617	0.96
305_T	309_L	0.617	0.96
331_K	340_L	0.617	0.96
39_V	55_P	0.616	0.96
281_V	285_R	0.615	0.96
9_E	168_N	0.614	0.96
175_T	228_D	0.614	0.96
41_S	51_V	0.613	0.96
91_D	141_T	0.612	0.96
163_G	210_P	0.611	0.96
10_G	14_N	0.611	0.96
65_A	69_T	0.610	0.96
291_E	294_K	0.610	0.96
299_V	307_Q	0.610	0.96
188_F	220_I	0.609	0.96
41_S	44_N	0.609	0.96
237_E	255_V	0.609	0.96
261_T	296_R	0.609	0.96
128_S	133_S	0.607	0.96
23_V	101_L	0.606	0.96
231_A	338_A	0.606	0.96
42_L	63_V	0.605	0.96
257_H	325_A	0.604	0.96
122_V	126_G	0.603	0.96
115_V	149_A	0.602	0.96
107_G	111_Q	0.601	0.95
39_V	58_L	0.600	0.95
122_V	237_E	0.597	0.95
18_G	24_G	0.597	0.95
113_Y	153_R	0.597	0.95
314_N	320_P	0.596	0.95
80_Q	199_G	0.596	0.95
130_G	134_S	0.594	0.95
257_H	322_D	0.593	0.95
305_T	308_D	0.593	0.95
227_P	230_P	0.591	0.95
298_F	301_P	0.588	0.95
104_P	115_V	0.587	0.95
12_R	149_A	0.587	0.95
180_I	183_A	0.586	0.95
38_T	65_A	0.584	0.95
279_Q	282_V	0.584	0.95
14_N	82_I	0.582	0.95
69_T	117_Q	0.581	0.95
132_G	266_E	0.581	0.95
172_P	177_A	0.575	0.94
224_E	227_P	0.574	0.94
130_G	266_E	0.574	0.94
188_F	223_L	0.572	0.94
19_Y	67_M	0.570	0.94
273_P	276_F	0.570	0.94
84_V	145_I	0.568	0.94
181_A	201_V	0.567	0.94
250_I	304_A	0.567	0.94
317_G	323_L	0.566	0.94
26_N	141_T	0.566	0.94
137_K	237_E	0.565	0.94
126_G	321_R	0.565	0.94
26_N	96_R	0.564	0.94
287_D	290_V	0.563	0.94
239_T	257_H	0.562	0.94
242_I	245_G	0.561	0.94
36_P	91_D	0.559	0.93
75_F	174_F	0.559	0.93
242_I	310_V	0.558	0.93
322_D	326_I	0.558	0.93
335_A	339_E	0.555	0.93
83_D	144_R	0.553	0.93
17_V	41_S	0.552	0.93
205_V	210_P	0.551	0.93
32_T	35_T	0.550	0.93
215_A	218_A	0.550	0.93
28_T	92_A	0.549	0.93
183_A	187_A	0.546	0.93
299_V	310_V	0.546	0.93
80_Q	237_E	0.545	0.93
157_F	162_G	0.545	0.93
127_A	134_S	0.544	0.92
139_H	237_E	0.542	0.92
12_R	115_V	0.542	0.92
229_A	247_D	0.539	0.92
121_S	146_P	0.539	0.92
20_G	29_G	0.539	0.92
179_R	226_E	0.538	0.92
41_S	46_L	0.538	0.92
246_G	254_A	0.538	0.92
127_A	130_G	0.537	0.92
3_K	176_T	0.535	0.92
35_T	39_V	0.535	0.92
6_A	203_V	0.534	0.92
107_G	115_V	0.533	0.92
278_G	281_V	0.533	0.92
236_N	328_Q	0.533	0.92
82_I	149_A	0.532	0.92
123_G	325_A	0.532	0.92
252_P	306_L	0.532	0.92
31_S	40_Q	0.530	0.91
132_G	284_P	0.529	0.91
25_L	28_T	0.529	0.91
330_L	337_Q	0.528	0.91
242_I	324_I	0.527	0.91
14_N	113_Y	0.527	0.91
255_V	330_L	0.527	0.91
252_P	259_N	0.527	0.91
96_R	136_T	0.526	0.91
306_L	314_N	0.525	0.91
19_Y	37_F	0.522	0.91
31_S	39_V	0.521	0.91
173_D	228_D	0.521	0.91
203_V	217_L	0.520	0.91
102_M	150_I	0.519	0.91
17_V	102_M	0.519	0.91
194_R	212_Q	0.518	0.91
74_P	157_F	0.515	0.90
330_L	335_A	0.514	0.90
233_V	337_Q	0.513	0.90
194_R	211_G	0.513	0.90
125_F	140_P	0.511	0.90
209_Y	216_F	0.511	0.90
256_A	314_N	0.510	0.90
133_S	263_T	0.510	0.90
34_Q	37_F	0.509	0.90
234_V	245_G	0.507	0.90
60_L	89_I	0.506	0.89
297_L	320_P	0.504	0.89
120_L	145_I	0.504	0.89
158_D	162_G	0.504	0.89
234_V	341_E	0.503	0.89
31_S	60_L	0.501	0.89
37_F	41_S	0.500	0.89

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1nhaA	1	0.1637	10.5	0.976	Contact Map
3dptA	2	0.1754	9.5	0.977	Contact Map
3smaA	2	0.1374	9.2	0.977	Contact Map
2zf9A	1	0.3158	6.7	0.978	Contact Map
2pfuA	1	0.2251	6.5	0.979	Contact Map
2xvcA	1	0.1637	6.4	0.979	Contact Map
2pmyA	4	0.1667	6.4	0.979	Contact Map
2i7aA	2	0.1374	5.7	0.979	Contact Map
4y66B	1	0.1696	5.6	0.979	Contact Map
2jwkA	2	0.1725	5.4	0.979	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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