GREMLIN Database
DUF63 - Membrane protein of unknown function DUF63
PFAM: PF01889 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 272 (268)
Sequences: 1334 (956)
Seq/√Len: 58.4
META: 0.795

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
190_A264_N5.6971.00
177_N250_L3.7831.00
91_F95_A3.7441.00
20_N23_N3.6661.00
24_T205_V3.5041.00
100_A122_I3.4361.00
191_T202_E3.0491.00
185_L225_L2.8861.00
18_G23_N2.7591.00
196_D217_A2.7201.00
200_Y267_R2.6921.00
9_F26_T2.5681.00
65_S69_V2.5611.00
236_D250_L2.5521.00
189_S221_F2.5371.00
96_I126_L2.5101.00
57_L119_L2.3211.00
194_G268_M2.3141.00
177_N236_D2.2891.00
219_V222_P2.0851.00
188_A228_A2.0801.00
5_I10_I2.0781.00
11_D15_Y2.0681.00
6_Y11_D2.0621.00
202_E207_P1.9351.00
62_L127_L1.9321.00
84_L88_G1.9321.00
195_I211_I1.9311.00
72_D263_R1.8741.00
198_L268_M1.8551.00
254_I258_L1.8411.00
8_Y20_N1.8240.99
76_L84_L1.7830.99
196_D216_T1.7710.99
63_F93_V1.7680.99
184_H260_P1.7550.99
68_R72_D1.7520.99
220_F223_L1.7310.99
5_I9_F1.7260.99
114_D117_K1.6670.99
56_A59_P1.6600.99
152_L189_S1.6200.99
190_A265_L1.6060.99
208_R212_E1.5980.99
144_W148_A1.5950.99
153_A182_F1.5680.98
261_G264_N1.5580.98
185_L188_A1.5560.98
210_L222_P1.5560.98
210_L219_V1.5510.98
63_F126_L1.5460.98
71_E85_I1.5440.98
65_S93_V1.5340.98
7_K11_D1.5060.98
69_V134_L1.5040.98
57_L97_T1.4970.98
124_L128_L1.4960.98
68_R203_K1.4940.98
29_I33_L1.4900.98
158_A162_L1.4900.98
181_L232_L1.4870.98
204_H263_R1.4570.97
251_L255_A1.4390.97
184_H227_V1.4280.97
165_R168_K1.4150.97
186_L265_L1.4070.97
232_L250_L1.3720.96
183_G254_I1.3480.96
211_I220_F1.3450.96
250_L256_L1.3430.96
82_Y88_G1.3350.96
229_L235_L1.3230.96
157_T178_L1.3170.95
42_L235_L1.2980.95
69_V266_L1.2950.95
66_A266_L1.2820.95
59_P123_G1.2740.94
191_T207_P1.2640.94
187_D202_E1.2580.94
259_G267_R1.2550.94
86_T92_T1.2520.94
63_F96_I1.2500.94
63_F130_N1.2460.94
260_P267_R1.2420.94
105_V109_R1.2400.94
125_L129_V1.2320.93
203_K261_G1.2320.93
214_T219_V1.2280.93
35_L252_A1.2170.93
60_F100_A1.2160.93
16_D203_K1.2130.93
66_A130_N1.2120.93
230_P234_L1.1890.92
71_E84_L1.1860.92
227_V231_V1.1820.92
76_L81_S1.1770.92
138_T141_V1.1700.92
145_V149_I1.1660.91
157_T182_F1.1590.91
89_I93_V1.1540.91
185_L228_A1.1530.91
38_V94_F1.1520.91
260_P264_N1.1510.91
182_F186_L1.1450.91
9_F27_Y1.1430.91
58_L62_L1.1430.91
149_I153_A1.1400.90
209_F213_L1.1380.90
163_L167_L1.1280.90
8_Y12_P1.1180.90
248_L252_A1.1140.89
41_L45_L1.1120.89
267_R272_V1.1090.89
73_A267_R1.1090.89
28_A205_V1.1080.89
183_G258_L1.1080.89
180_V254_I1.1070.89
68_R86_T1.1050.89
246_R251_L1.0930.88
188_A221_F1.0790.88
187_D191_T1.0630.87
156_L225_L1.0600.87
104_S118_P1.0550.87
48_K52_R1.0530.87
34_A38_V1.0510.86
231_V253_I1.0510.86
34_A58_L1.0420.86
37_G41_L1.0390.86
8_Y22_V1.0370.86
71_E81_S1.0360.86
192_F221_F1.0270.85
207_P211_I1.0250.85
69_V264_N1.0230.85
69_V267_R1.0230.85
14_V17_P1.0190.85
107_L111_L1.0030.84
42_L94_F1.0010.84
194_G272_V0.9870.83
104_S119_L0.9850.83
94_F98_L0.9790.82
194_G200_Y0.9750.82
154_T182_F0.9720.82
63_F127_L0.9660.81
72_D204_H0.9580.81
192_F196_D0.9520.80
146_L265_L0.9500.80
153_A156_L0.9490.80
164_L197_F0.9450.80
106_K110_K0.9430.80
211_I214_T0.9430.80
150_L153_A0.9420.80
21_I24_T0.9370.79
184_H257_G0.9370.79
21_I205_V0.9350.79
89_I259_G0.9280.79
153_A186_L0.9150.78
194_G267_R0.9100.77
227_V230_P0.9000.77
71_E79_P0.8980.76
186_L190_A0.8960.76
120_A124_L0.8950.76
239_V242_E0.8950.76
43_K128_L0.8920.76
192_F218_W0.8900.76
49_I52_R0.8890.76
44_R113_K0.8870.76
121_A125_L0.8840.75
239_V246_R0.8830.75
180_V184_H0.8820.75
30_I34_A0.8770.75
72_D76_L0.8700.74
181_L185_L0.8620.73
228_A232_L0.8620.73
50_D53_F0.8590.73
70_L130_N0.8580.73
177_N232_L0.8570.73
236_D246_R0.8560.73
137_T140_I0.8560.73
24_T28_A0.8560.73
264_N267_R0.8540.73
210_L223_L0.8520.73
220_F224_K0.8480.72
190_A195_I0.8470.72
207_P214_T0.8440.72
47_I253_I0.8420.72
68_R263_R0.8390.71
108_E112_V0.8350.71
90_Y105_V0.8350.71
22_V26_T0.8340.71
80_L206_L0.8330.71
235_L249_L0.8310.71
164_L167_L0.8290.70
100_A104_S0.8290.70
84_L94_F0.8260.70
126_L130_N0.8240.70
201_G208_R0.8240.70
211_I217_A0.8230.70
198_L211_I0.8220.70
78_P81_S0.8210.70
11_D14_V0.8180.69
221_F228_A0.8160.69
174_G177_N0.8050.68
127_L131_L0.8020.68
213_L219_V0.8020.68
80_L84_L0.8000.68
64_G90_Y0.7990.68
42_L99_A0.7900.67
194_G198_L0.7900.67
211_I219_V0.7890.67
263_R267_R0.7880.67
71_E88_G0.7870.66
68_R85_I0.7870.66
98_L102_L0.7810.66
33_L37_G0.7780.66
119_L123_G0.7770.65
148_A198_L0.7740.65
68_R204_H0.7720.65
69_V72_D0.7680.65
58_L258_L0.7670.64
20_N24_T0.7640.64
35_L203_K0.7610.64
189_S220_F0.7600.64
152_L185_L0.7560.63
202_E211_I0.7520.63
215_G219_V0.7520.63
97_T119_L0.7510.63
108_E115_Y0.7480.63
258_L261_G0.7450.62
94_F97_T0.7430.62
146_L269_T0.7420.62
60_F119_L0.7410.62
146_L199_G0.7390.62
21_I150_L0.7380.61
62_L163_L0.7330.61
52_R114_D0.7320.61
89_I200_Y0.7290.61
234_L237_K0.7290.61
11_D17_P0.7280.60
154_T159_L0.7260.60
175_K178_L0.7240.60
159_L165_R0.7180.59
153_A157_T0.7110.59
223_L228_A0.7090.58
73_A200_Y0.7040.58
72_D86_T0.7020.58
103_L106_K0.7000.57
255_A259_G0.7000.57
245_L264_N0.6990.57
25_L166_R0.6980.57
145_V234_L0.6980.57
76_L134_L0.6970.57
5_I36_Y0.6950.57
21_I25_L0.6940.57
65_S259_G0.6940.57
69_V94_F0.6930.57
65_S266_L0.6910.56
261_G265_L0.6900.56
138_T142_N0.6870.56
195_I200_Y0.6790.55
175_K181_L0.6780.55
136_L139_G0.6770.55
212_E215_G0.6770.55
31_L88_G0.6760.55
192_F197_F0.6730.54
159_L162_L0.6720.54
146_L149_I0.6720.54
95_A118_P0.6700.54
42_L84_L0.6690.54
220_F264_N0.6680.54
181_L265_L0.6650.54
129_V213_L0.6650.54
168_K210_L0.6640.53
81_S88_G0.6590.53
207_P227_V0.6540.52
12_P20_N0.6540.52
187_D204_H0.6520.52
86_T259_G0.6510.52
103_L111_L0.6510.52
36_Y40_K0.6490.52
160_V164_L0.6440.51
66_A127_L0.6440.51
26_T31_L0.6440.51
177_N255_A0.6440.51
97_T101_L0.6400.51
215_G231_V0.6390.51
229_L232_L0.6370.50
202_E260_P0.6360.50
67_L130_N0.6350.50
149_I265_L0.6330.50
86_T103_L0.6330.50
42_L49_I0.6300.50
210_L238_Y0.6240.49
115_Y119_L0.6200.49
120_A126_L0.6200.49
9_F23_N0.6200.49
239_V253_I0.6190.48
54_V254_I0.6170.48
65_S100_A0.6160.48
58_L254_I0.6160.48
220_F228_A0.6150.48
98_L101_L0.6120.48
141_V151_G0.6100.48
195_I217_A0.6070.47
174_G183_G0.6060.47
10_I14_V0.6050.47
148_A193_V0.6050.47
156_L163_L0.6030.47
72_D207_P0.6030.47
34_A255_A0.6030.47
145_V148_A0.6020.47
95_A102_L0.6010.47
123_G127_L0.6000.46
151_G226_A0.5990.46
65_S94_F0.5990.46
37_G170_N0.5980.46
181_L230_P0.5940.46
52_R125_L0.5910.45
211_I216_T0.5890.45
12_P23_N0.5880.45
56_A119_L0.5880.45
216_T272_V0.5860.45
200_Y204_H0.5840.45
123_G126_L0.5830.45
249_L261_G0.5820.44
47_I251_L0.5820.44
42_L47_I0.5810.44
220_F267_R0.5790.44
152_L155_V0.5780.44
254_I260_P0.5780.44
82_Y85_I0.5770.44
147_L155_V0.5770.44
207_P250_L0.5770.44
18_G31_L0.5760.44
85_I91_F0.5760.44
12_P16_D0.5750.44
195_I264_N0.5740.44
25_L46_K0.5740.44
39_Y160_V0.5700.43
247_N251_L0.5690.43
62_L147_L0.5680.43
94_F207_P0.5670.43
72_D266_L0.5670.43
200_Y272_V0.5660.43
48_K210_L0.5660.43
102_L162_L0.5650.43
162_L165_R0.5650.43
118_P121_A0.5640.42
137_T150_L0.5630.42
55_L168_K0.5630.42
46_K49_I0.5610.42
30_I102_L0.5570.42
12_P203_K0.5560.42
72_D85_I0.5560.42
156_L160_V0.5540.41
51_R175_K0.5520.41
147_L151_G0.5520.41
203_K259_G0.5470.41
191_T211_I0.5460.41
239_V249_L0.5460.41
160_V174_G0.5450.40
60_F190_A0.5440.40
17_P20_N0.5440.40
33_L97_T0.5440.40
89_I267_R0.5440.40
173_T235_L0.5410.40
186_L205_V0.5400.40
18_G207_P0.5390.40
63_F89_I0.5390.40
191_T268_M0.5380.40
19_Y85_I0.5360.39
33_L171_A0.5360.39
76_L192_F0.5350.39
46_K95_A0.5340.39
120_A135_L0.5310.39
57_L92_T0.5280.39
262_T265_L0.5280.39
6_Y14_V0.5270.39
147_L150_L0.5270.39
223_L231_V0.5240.38
70_L86_T0.5230.38
71_E78_P0.5220.38
103_L114_D0.5220.38
109_R113_K0.5200.38
214_T217_A0.5190.38
61_I64_G0.5190.38
65_S208_R0.5190.38
65_S256_L0.5190.38
95_A99_A0.5170.37
177_N180_V0.5170.37
187_D224_K0.5130.37
184_H221_F0.5130.37
209_F261_G0.5120.37
53_F251_L0.5090.37
128_L163_L0.5080.37
142_N154_T0.5040.36
93_V241_E0.5030.36
58_L108_E0.5030.36
112_V124_L0.5020.36
10_I15_Y0.5010.36
7_K15_Y0.5010.36
157_T181_L0.5000.36
207_P220_F0.5000.36
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4cz8A 2 0.8676 15.7 0.962 Contact Map
3wajA 1 0.8015 11.7 0.964 Contact Map
4czbA 2 0.8566 7 0.968 Contact Map
2k1oA 2 0.0956 4.3 0.971 Contact Map
4he8L 1 0.4522 3.5 0.972 Contact Map
2k1eA 3 0.364 3.3 0.973 Contact Map
3j81l 1 0 3.2 0.973 Contact Map
4xnjA 1 0.8162 2.7 0.974 Contact Map
3rkoN 1 0.4632 2.4 0.974 Contact Map
3wu2T 1 0.1066 2.2 0.975 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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