DUF63 - PFAM28P - GREMLIN Contacts

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DUF63 - Membrane protein of unknown function DUF63

PFAM:	PF01889	Sequences: ISDFIYKYFIDPIVYDPGYNIVNTLTYAIILLLALYGVYKLLKRLKIKIDRRFVLALLPFILFGSALRVLEDAGILPPPLSYLLITPGIYFTVFAITLAALLLSVKLERKLVKDYEKPLAAIGLLLLLVNLALLLLTTGIVNPWVLLAILGLATVLTALVWLLLRRLKINALTGKLNLLVLFGHLLDASATFVGIDFLGYGEKHVLPRFLIELTGTAWVFFPLKLAVALPVLYLLDKYVEEEPPLRNLLLLAIIALGLGPGTRNLLRMTLGV	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_10
Length:	272 (268)
Sequences:	1334 (956)
Seq/√Len:	58.4
META:	0.795

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
190_A	264_N	5.697	1.00
177_N	250_L	3.783	1.00
91_F	95_A	3.744	1.00
20_N	23_N	3.666	1.00
24_T	205_V	3.504	1.00
100_A	122_I	3.436	1.00
191_T	202_E	3.049	1.00
185_L	225_L	2.886	1.00
18_G	23_N	2.759	1.00
196_D	217_A	2.720	1.00
200_Y	267_R	2.692	1.00
9_F	26_T	2.568	1.00
65_S	69_V	2.561	1.00
236_D	250_L	2.552	1.00
189_S	221_F	2.537	1.00
96_I	126_L	2.510	1.00
57_L	119_L	2.321	1.00
194_G	268_M	2.314	1.00
177_N	236_D	2.289	1.00
219_V	222_P	2.085	1.00
188_A	228_A	2.080	1.00
5_I	10_I	2.078	1.00
11_D	15_Y	2.068	1.00
6_Y	11_D	2.062	1.00
202_E	207_P	1.935	1.00
62_L	127_L	1.932	1.00
84_L	88_G	1.932	1.00
195_I	211_I	1.931	1.00
72_D	263_R	1.874	1.00
198_L	268_M	1.855	1.00
254_I	258_L	1.841	1.00
8_Y	20_N	1.824	0.99
76_L	84_L	1.783	0.99
196_D	216_T	1.771	0.99
63_F	93_V	1.768	0.99
184_H	260_P	1.755	0.99
68_R	72_D	1.752	0.99
220_F	223_L	1.731	0.99
5_I	9_F	1.726	0.99
114_D	117_K	1.667	0.99
56_A	59_P	1.660	0.99
152_L	189_S	1.620	0.99
190_A	265_L	1.606	0.99
208_R	212_E	1.598	0.99
144_W	148_A	1.595	0.99
153_A	182_F	1.568	0.98
261_G	264_N	1.558	0.98
185_L	188_A	1.556	0.98
210_L	222_P	1.556	0.98
210_L	219_V	1.551	0.98
63_F	126_L	1.546	0.98
71_E	85_I	1.544	0.98
65_S	93_V	1.534	0.98
7_K	11_D	1.506	0.98
69_V	134_L	1.504	0.98
57_L	97_T	1.497	0.98
124_L	128_L	1.496	0.98
68_R	203_K	1.494	0.98
29_I	33_L	1.490	0.98
158_A	162_L	1.490	0.98
181_L	232_L	1.487	0.98
204_H	263_R	1.457	0.97
251_L	255_A	1.439	0.97
184_H	227_V	1.428	0.97
165_R	168_K	1.415	0.97
186_L	265_L	1.407	0.97
232_L	250_L	1.372	0.96
183_G	254_I	1.348	0.96
211_I	220_F	1.345	0.96
250_L	256_L	1.343	0.96
82_Y	88_G	1.335	0.96
229_L	235_L	1.323	0.96
157_T	178_L	1.317	0.95
42_L	235_L	1.298	0.95
69_V	266_L	1.295	0.95
66_A	266_L	1.282	0.95
59_P	123_G	1.274	0.94
191_T	207_P	1.264	0.94
187_D	202_E	1.258	0.94
259_G	267_R	1.255	0.94
86_T	92_T	1.252	0.94
63_F	96_I	1.250	0.94
63_F	130_N	1.246	0.94
260_P	267_R	1.242	0.94
105_V	109_R	1.240	0.94
125_L	129_V	1.232	0.93
203_K	261_G	1.232	0.93
214_T	219_V	1.228	0.93
35_L	252_A	1.217	0.93
60_F	100_A	1.216	0.93
16_D	203_K	1.213	0.93
66_A	130_N	1.212	0.93
230_P	234_L	1.189	0.92
71_E	84_L	1.186	0.92
227_V	231_V	1.182	0.92
76_L	81_S	1.177	0.92
138_T	141_V	1.170	0.92
145_V	149_I	1.166	0.91
157_T	182_F	1.159	0.91
89_I	93_V	1.154	0.91
185_L	228_A	1.153	0.91
38_V	94_F	1.152	0.91
260_P	264_N	1.151	0.91
182_F	186_L	1.145	0.91
9_F	27_Y	1.143	0.91
58_L	62_L	1.143	0.91
149_I	153_A	1.140	0.90
209_F	213_L	1.138	0.90
163_L	167_L	1.128	0.90
8_Y	12_P	1.118	0.90
248_L	252_A	1.114	0.89
41_L	45_L	1.112	0.89
267_R	272_V	1.109	0.89
73_A	267_R	1.109	0.89
28_A	205_V	1.108	0.89
183_G	258_L	1.108	0.89
180_V	254_I	1.107	0.89
68_R	86_T	1.105	0.89
246_R	251_L	1.093	0.88
188_A	221_F	1.079	0.88
187_D	191_T	1.063	0.87
156_L	225_L	1.060	0.87
104_S	118_P	1.055	0.87
48_K	52_R	1.053	0.87
34_A	38_V	1.051	0.86
231_V	253_I	1.051	0.86
34_A	58_L	1.042	0.86
37_G	41_L	1.039	0.86
8_Y	22_V	1.037	0.86
71_E	81_S	1.036	0.86
192_F	221_F	1.027	0.85
207_P	211_I	1.025	0.85
69_V	264_N	1.023	0.85
69_V	267_R	1.023	0.85
14_V	17_P	1.019	0.85
107_L	111_L	1.003	0.84
42_L	94_F	1.001	0.84
194_G	272_V	0.987	0.83
104_S	119_L	0.985	0.83
94_F	98_L	0.979	0.82
194_G	200_Y	0.975	0.82
154_T	182_F	0.972	0.82
63_F	127_L	0.966	0.81
72_D	204_H	0.958	0.81
192_F	196_D	0.952	0.80
146_L	265_L	0.950	0.80
153_A	156_L	0.949	0.80
164_L	197_F	0.945	0.80
106_K	110_K	0.943	0.80
211_I	214_T	0.943	0.80
150_L	153_A	0.942	0.80
21_I	24_T	0.937	0.79
184_H	257_G	0.937	0.79
21_I	205_V	0.935	0.79
89_I	259_G	0.928	0.79
153_A	186_L	0.915	0.78
194_G	267_R	0.910	0.77
227_V	230_P	0.900	0.77
71_E	79_P	0.898	0.76
186_L	190_A	0.896	0.76
120_A	124_L	0.895	0.76
239_V	242_E	0.895	0.76
43_K	128_L	0.892	0.76
192_F	218_W	0.890	0.76
49_I	52_R	0.889	0.76
44_R	113_K	0.887	0.76
121_A	125_L	0.884	0.75
239_V	246_R	0.883	0.75
180_V	184_H	0.882	0.75
30_I	34_A	0.877	0.75
72_D	76_L	0.870	0.74
181_L	185_L	0.862	0.73
228_A	232_L	0.862	0.73
50_D	53_F	0.859	0.73
70_L	130_N	0.858	0.73
177_N	232_L	0.857	0.73
236_D	246_R	0.856	0.73
137_T	140_I	0.856	0.73
24_T	28_A	0.856	0.73
264_N	267_R	0.854	0.73
210_L	223_L	0.852	0.73
220_F	224_K	0.848	0.72
190_A	195_I	0.847	0.72
207_P	214_T	0.844	0.72
47_I	253_I	0.842	0.72
68_R	263_R	0.839	0.71
108_E	112_V	0.835	0.71
90_Y	105_V	0.835	0.71
22_V	26_T	0.834	0.71
80_L	206_L	0.833	0.71
235_L	249_L	0.831	0.71
164_L	167_L	0.829	0.70
100_A	104_S	0.829	0.70
84_L	94_F	0.826	0.70
126_L	130_N	0.824	0.70
201_G	208_R	0.824	0.70
211_I	217_A	0.823	0.70
198_L	211_I	0.822	0.70
78_P	81_S	0.821	0.70
11_D	14_V	0.818	0.69
221_F	228_A	0.816	0.69
174_G	177_N	0.805	0.68
127_L	131_L	0.802	0.68
213_L	219_V	0.802	0.68
80_L	84_L	0.800	0.68
64_G	90_Y	0.799	0.68
42_L	99_A	0.790	0.67
194_G	198_L	0.790	0.67
211_I	219_V	0.789	0.67
263_R	267_R	0.788	0.67
71_E	88_G	0.787	0.66
68_R	85_I	0.787	0.66
98_L	102_L	0.781	0.66
33_L	37_G	0.778	0.66
119_L	123_G	0.777	0.65
148_A	198_L	0.774	0.65
68_R	204_H	0.772	0.65
69_V	72_D	0.768	0.65
58_L	258_L	0.767	0.64
20_N	24_T	0.764	0.64
35_L	203_K	0.761	0.64
189_S	220_F	0.760	0.64
152_L	185_L	0.756	0.63
202_E	211_I	0.752	0.63
215_G	219_V	0.752	0.63
97_T	119_L	0.751	0.63
108_E	115_Y	0.748	0.63
258_L	261_G	0.745	0.62
94_F	97_T	0.743	0.62
146_L	269_T	0.742	0.62
60_F	119_L	0.741	0.62
146_L	199_G	0.739	0.62
21_I	150_L	0.738	0.61
62_L	163_L	0.733	0.61
52_R	114_D	0.732	0.61
89_I	200_Y	0.729	0.61
234_L	237_K	0.729	0.61
11_D	17_P	0.728	0.60
154_T	159_L	0.726	0.60
175_K	178_L	0.724	0.60
159_L	165_R	0.718	0.59
153_A	157_T	0.711	0.59
223_L	228_A	0.709	0.58
73_A	200_Y	0.704	0.58
72_D	86_T	0.702	0.58
103_L	106_K	0.700	0.57
255_A	259_G	0.700	0.57
245_L	264_N	0.699	0.57
25_L	166_R	0.698	0.57
145_V	234_L	0.698	0.57
76_L	134_L	0.697	0.57
5_I	36_Y	0.695	0.57
21_I	25_L	0.694	0.57
65_S	259_G	0.694	0.57
69_V	94_F	0.693	0.57
65_S	266_L	0.691	0.56
261_G	265_L	0.690	0.56
138_T	142_N	0.687	0.56
195_I	200_Y	0.679	0.55
175_K	181_L	0.678	0.55
136_L	139_G	0.677	0.55
212_E	215_G	0.677	0.55
31_L	88_G	0.676	0.55
192_F	197_F	0.673	0.54
159_L	162_L	0.672	0.54
146_L	149_I	0.672	0.54
95_A	118_P	0.670	0.54
42_L	84_L	0.669	0.54
220_F	264_N	0.668	0.54
181_L	265_L	0.665	0.54
129_V	213_L	0.665	0.54
168_K	210_L	0.664	0.53
81_S	88_G	0.659	0.53
207_P	227_V	0.654	0.52
12_P	20_N	0.654	0.52
187_D	204_H	0.652	0.52
86_T	259_G	0.651	0.52
103_L	111_L	0.651	0.52
36_Y	40_K	0.649	0.52
160_V	164_L	0.644	0.51
66_A	127_L	0.644	0.51
26_T	31_L	0.644	0.51
177_N	255_A	0.644	0.51
97_T	101_L	0.640	0.51
215_G	231_V	0.639	0.51
229_L	232_L	0.637	0.50
202_E	260_P	0.636	0.50
67_L	130_N	0.635	0.50
149_I	265_L	0.633	0.50
86_T	103_L	0.633	0.50
42_L	49_I	0.630	0.50
210_L	238_Y	0.624	0.49
115_Y	119_L	0.620	0.49
120_A	126_L	0.620	0.49
9_F	23_N	0.620	0.49
239_V	253_I	0.619	0.48
54_V	254_I	0.617	0.48
65_S	100_A	0.616	0.48
58_L	254_I	0.616	0.48
220_F	228_A	0.615	0.48
98_L	101_L	0.612	0.48
141_V	151_G	0.610	0.48
195_I	217_A	0.607	0.47
174_G	183_G	0.606	0.47
10_I	14_V	0.605	0.47
148_A	193_V	0.605	0.47
156_L	163_L	0.603	0.47
72_D	207_P	0.603	0.47
34_A	255_A	0.603	0.47
145_V	148_A	0.602	0.47
95_A	102_L	0.601	0.47
123_G	127_L	0.600	0.46
151_G	226_A	0.599	0.46
65_S	94_F	0.599	0.46
37_G	170_N	0.598	0.46
181_L	230_P	0.594	0.46
52_R	125_L	0.591	0.45
211_I	216_T	0.589	0.45
12_P	23_N	0.588	0.45
56_A	119_L	0.588	0.45
216_T	272_V	0.586	0.45
200_Y	204_H	0.584	0.45
123_G	126_L	0.583	0.45
249_L	261_G	0.582	0.44
47_I	251_L	0.582	0.44
42_L	47_I	0.581	0.44
220_F	267_R	0.579	0.44
152_L	155_V	0.578	0.44
254_I	260_P	0.578	0.44
82_Y	85_I	0.577	0.44
147_L	155_V	0.577	0.44
207_P	250_L	0.577	0.44
18_G	31_L	0.576	0.44
85_I	91_F	0.576	0.44
12_P	16_D	0.575	0.44
195_I	264_N	0.574	0.44
25_L	46_K	0.574	0.44
39_Y	160_V	0.570	0.43
247_N	251_L	0.569	0.43
62_L	147_L	0.568	0.43
94_F	207_P	0.567	0.43
72_D	266_L	0.567	0.43
200_Y	272_V	0.566	0.43
48_K	210_L	0.566	0.43
102_L	162_L	0.565	0.43
162_L	165_R	0.565	0.43
118_P	121_A	0.564	0.42
137_T	150_L	0.563	0.42
55_L	168_K	0.563	0.42
46_K	49_I	0.561	0.42
30_I	102_L	0.557	0.42
12_P	203_K	0.556	0.42
72_D	85_I	0.556	0.42
156_L	160_V	0.554	0.41
51_R	175_K	0.552	0.41
147_L	151_G	0.552	0.41
203_K	259_G	0.547	0.41
191_T	211_I	0.546	0.41
239_V	249_L	0.546	0.41
160_V	174_G	0.545	0.40
60_F	190_A	0.544	0.40
17_P	20_N	0.544	0.40
33_L	97_T	0.544	0.40
89_I	267_R	0.544	0.40
173_T	235_L	0.541	0.40
186_L	205_V	0.540	0.40
18_G	207_P	0.539	0.40
63_F	89_I	0.539	0.40
191_T	268_M	0.538	0.40
19_Y	85_I	0.536	0.39
33_L	171_A	0.536	0.39
76_L	192_F	0.535	0.39
46_K	95_A	0.534	0.39
120_A	135_L	0.531	0.39
57_L	92_T	0.528	0.39
262_T	265_L	0.528	0.39
6_Y	14_V	0.527	0.39
147_L	150_L	0.527	0.39
223_L	231_V	0.524	0.38
70_L	86_T	0.523	0.38
71_E	78_P	0.522	0.38
103_L	114_D	0.522	0.38
109_R	113_K	0.520	0.38
214_T	217_A	0.519	0.38
61_I	64_G	0.519	0.38
65_S	208_R	0.519	0.38
65_S	256_L	0.519	0.38
95_A	99_A	0.517	0.37
177_N	180_V	0.517	0.37
187_D	224_K	0.513	0.37
184_H	221_F	0.513	0.37
209_F	261_G	0.512	0.37
53_F	251_L	0.509	0.37
128_L	163_L	0.508	0.37
142_N	154_T	0.504	0.36
93_V	241_E	0.503	0.36
58_L	108_E	0.503	0.36
112_V	124_L	0.502	0.36
10_I	15_Y	0.501	0.36
7_K	15_Y	0.501	0.36
157_T	181_L	0.500	0.36
207_P	220_F	0.500	0.36

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4cz8A	2	0.8676	15.7	0.962	Contact Map
3wajA	1	0.8015	11.7	0.964	Contact Map
4czbA	2	0.8566	7	0.968	Contact Map
2k1oA	2	0.0956	4.3	0.971	Contact Map
4he8L	1	0.4522	3.5	0.972	Contact Map
2k1eA	3	0.364	3.3	0.973	Contact Map
3j81l	1	0	3.2	0.973	Contact Map
4xnjA	1	0.8162	2.7	0.974	Contact Map
3rkoN	1	0.4632	2.4	0.974	Contact Map
3wu2T	1	0.1066	2.2	0.975	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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