GREMLIN Database
NUCS1 - Endonuclease NucS 1
UniProt: Q9HSL7 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 240 (208)
Sequences: 459 (183)
Seq/√Len: 12.7

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
138_P161_I5.9211.00
206_R228_T5.3431.00
192_V227_L4.6401.00
61_V67_G4.5271.00
138_P163_G3.6370.99
60_V102_V3.5330.99
61_V66_S3.2290.98
94_G98_G2.7750.94
47_A105_D2.7340.94
129_A132_D2.7200.94
29_L52_L2.6550.93
36_T44_D2.6330.92
32_S49_V2.5630.91
141_V173_V2.4740.90
137_T140_L2.3840.88
140_L145_F2.2570.85
61_V64_G2.2500.85
165_D206_R2.2010.83
138_P145_F2.1950.83
185_A218_T2.1360.81
162_Y170_V2.1280.81
52_L62_V2.1140.81
138_P232_V2.1060.80
208_I228_T2.0760.79
141_V232_V2.0330.78
153_D195_L1.9180.74
98_G103_R1.9060.73
75_S117_G1.8650.71
181_G215_T1.7870.68
138_P175_L1.7690.67
93_G96_T1.7430.66
173_V208_I1.7220.65
223_A228_T1.7150.65
49_V108_H1.6740.63
141_V145_F1.6630.62
145_F163_G1.6520.62
142_E145_F1.6460.61
145_F161_I1.6450.61
31_G104_F1.6380.61
32_S105_D1.6160.60
28_S51_T1.6120.60
64_G67_G1.5620.57
53_L66_S1.5340.56
109_T113_F1.5260.55
161_I232_V1.4380.51
142_E206_R1.4320.51
52_L102_V1.4130.50
89_V93_G1.3860.48
92_D97_A1.3720.47
178_V213_A1.3630.47
75_S96_T1.3630.47
89_V98_G1.3570.47
65_A178_V1.3560.47
144_G165_D1.3380.46
89_V92_D1.3340.46
98_G137_T1.2980.44
98_G105_D1.2850.43
129_A181_G1.2790.43
33_C45_L1.2780.43
43_R74_A1.2740.42
163_G175_L1.2660.42
89_V96_T1.2630.42
86_G101_R1.2450.41
196_R205_V1.2440.41
45_L121_T1.2350.41
161_I175_L1.2310.40
208_I215_T1.2260.40
94_G102_V1.2070.39
141_V228_T1.2040.39
197_R224_D1.1960.39
89_V101_R1.1870.38
125_G190_R1.1700.37
139_D143_P1.1690.37
143_P166_A1.1630.37
93_G98_G1.1620.37
106_A139_D1.1230.35
195_L232_V1.1110.35
52_L95_H1.1000.34
128_A131_K1.0940.34
93_G169_T1.0770.33
59_T210_V1.0700.33
153_D162_Y1.0560.32
58_D63_H1.0490.32
138_P204_T1.0420.31
145_F175_L1.0380.31
52_L104_F1.0360.31
90_A93_G1.0310.31
47_A102_V1.0270.31
163_G170_V1.0260.31
100_L221_L1.0240.31
61_V95_H1.0160.30
196_R204_T1.0140.30
163_G232_V0.9960.29
172_A198_T0.9950.29
42_Q132_D0.9840.29
42_Q137_T0.9790.29
219_R226_G0.9780.29
51_T95_H0.9730.28
29_L35_A0.9680.28
53_L67_G0.9600.28
89_V223_A0.9600.28
35_A63_H0.9590.28
107_V137_T0.9470.27
113_F181_G0.9430.27
84_A167_D0.9400.27
80_T232_V0.9320.27
101_R132_D0.9270.27
47_A122_T0.9140.26
32_S106_A0.9070.26
83_A86_G0.8970.25
68_R186_S0.8940.25
75_S181_G0.8930.25
91_C96_T0.8900.25
156_A194_A0.8800.25
90_A96_T0.8790.25
145_F206_R0.8770.25
68_R126_T0.8710.24
35_A45_L0.8690.24
26_T36_T0.8660.24
49_V117_G0.8630.24
30_I104_F0.8630.24
31_G182_P0.8600.24
49_V106_A0.8590.24
68_R77_G0.8540.24
76_G173_V0.8540.24
130_L158_P0.8430.23
38_T111_T0.8410.23
140_L163_G0.8340.23
169_T206_R0.8300.23
126_T198_T0.8210.22
159_I221_L0.8170.22
138_P196_R0.8120.22
75_S227_L0.8080.22
99_A103_R0.8070.22
141_V161_I0.8030.22
161_I196_R0.8030.22
131_K150_T0.8020.22
71_D117_G0.7940.22
145_F232_V0.7920.21
86_G104_F0.7840.21
184_A209_L0.7830.21
61_V90_A0.7800.21
93_G138_P0.7750.21
31_G52_L0.7730.21
148_L162_Y0.7720.21
47_A90_A0.7640.20
116_A119_D0.7570.20
41_S123_V0.7560.20
146_Q203_A0.7460.20
121_T178_V0.7380.20
35_A58_D0.7370.20
63_H192_V0.7350.20
60_V112_A0.7350.20
49_V98_G0.7320.19
67_G93_G0.7290.19
49_V232_V0.7290.19
146_Q169_T0.7250.19
122_T181_G0.7240.19
209_L213_A0.7230.19
219_R223_A0.7230.19
154_T159_I0.7220.19
38_T99_A0.7190.19
93_G213_A0.7170.19
189_Q225_R0.7170.19
200_H203_A0.7140.19
102_V233_S0.7130.19
38_T98_G0.7120.19
103_R146_Q0.7100.19
49_V112_A0.7080.19
121_T125_G0.7080.19
65_A121_T0.7040.19
208_I230_S0.6990.18
75_S93_G0.6990.18
164_R170_V0.6980.18
163_G173_V0.6980.18
90_A181_G0.6940.18
48_G71_D0.6920.18
45_L50_R0.6900.18
48_G74_A0.6880.18
34_V47_A0.6860.18
59_T68_R0.6850.18
42_Q140_L0.6850.18
58_D75_S0.6840.18
98_G163_G0.6810.18
59_T173_V0.6800.18
38_T101_R0.6790.18
53_L146_Q0.6780.18
43_R212_P0.6750.18
51_T56_P0.6740.18
99_A107_V0.6720.18
114_D208_I0.6710.18
154_T221_L0.6710.18
88_T96_T0.6690.17
92_D102_V0.6680.17
37_L72_A0.6640.17
91_C98_G0.6630.17
48_G117_G0.6610.17
58_D218_T0.6600.17
148_L170_V0.6590.17
30_I173_V0.6550.17
107_V165_D0.6520.17
63_H178_V0.6510.17
145_F173_V0.6500.17
206_R230_S0.6480.17
53_L75_S0.6470.17
102_V166_A0.6470.17
84_A228_T0.6430.17
129_A175_L0.6360.17
38_T103_R0.6320.16
81_V155_P0.6320.16
142_E165_D0.6320.16
139_D146_Q0.6310.16
62_V86_G0.6290.16
153_D205_V0.6270.16
35_A53_L0.6260.16
193_A232_V0.6250.16
132_D141_V0.6240.16
128_A159_I0.6230.16
88_T98_G0.6220.16
83_A109_T0.6210.16
90_A102_V0.6210.16
140_L232_V0.6200.16
38_T42_Q0.6170.16
32_S108_H0.6160.16
115_A226_G0.6150.16
53_L58_D0.6100.16
97_A209_L0.6070.16
90_A94_G0.6050.16
32_S86_G0.6000.16
46_A161_I0.5940.15
42_Q129_A0.5930.15
165_D169_T0.5930.15
86_G168_G0.5910.15
125_G159_I0.5890.15
38_T167_D0.5890.15
48_G77_G0.5880.15
170_V203_A0.5880.15
97_A162_Y0.5870.15
94_G103_R0.5820.15
93_G206_R0.5820.15
180_A184_A0.5820.15
154_T183_A0.5820.15
61_V91_C0.5810.15
117_G228_T0.5790.15
90_A97_A0.5790.15
141_V226_G0.5780.15
215_T227_L0.5780.15
146_Q181_G0.5770.15
37_L40_R0.5770.15
128_A134_V0.5770.15
170_V232_V0.5770.15
81_V86_G0.5760.15
154_T158_P0.5760.15
52_L93_G0.5760.15
54_W60_V0.5740.15
88_T94_G0.5740.15
173_V210_V0.5720.15
184_A234_P0.5720.15
143_P153_D0.5720.15
77_G182_P0.5710.15
143_P204_T0.5690.15
138_P142_E0.5690.15
153_D220_R0.5680.15
111_T143_P0.5670.15
45_L186_S0.5640.15
154_T211_A0.5610.14
123_V126_T0.5580.14
66_S89_V0.5580.14
75_S102_V0.5580.14
86_G114_D0.5570.14
30_I44_D0.5570.14
87_L107_V0.5560.14
98_G230_S0.5540.14
62_V93_G0.5530.14
28_S95_H0.5520.14
93_G105_D0.5490.14
38_T132_D0.5490.14
116_A225_R0.5450.14
38_T95_H0.5440.14
66_S93_G0.5410.14
80_T91_C0.5400.14
34_V105_D0.5390.14
54_W146_Q0.5390.14
133_R149_A0.5380.14
63_H227_L0.5380.14
123_V131_K0.5370.14
123_V234_P0.5350.14
133_R178_V0.5340.14
61_V121_T0.5300.14
143_P167_D0.5300.14
149_A154_T0.5290.14
156_A173_V0.5280.14
106_A128_A0.5270.14
146_Q166_A0.5230.14
90_A99_A0.5220.14
67_G200_H0.5200.13
54_W103_R0.5170.13
134_V147_P0.5170.13
60_V119_D0.5160.13
89_V167_D0.5130.13
33_C50_R0.5130.13
128_A205_V0.5120.13
28_S164_R0.5110.13
155_P159_I0.5100.13
209_L215_T0.5080.13
132_D181_G0.5070.13
67_G140_L0.5060.13
122_T138_P0.5050.13
72_A221_L0.5040.13
122_T230_S0.5030.13
32_S47_A0.5000.13
107_V172_A0.5000.13
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2vldA 2 0.9417 100 0.281 Contact Map
1y88A 5 0.4375 97.3 0.919 Contact Map
2wcwA 2 0.4042 95.2 0.935 Contact Map
3h1tA 1 0.4042 93.9 0.939 Contact Map
1gefA 2 0.4417 92.5 0.942 Contact Map
2eo0A 2 0.425 89.7 0.946 Contact Map
1ob8A 1 0.3875 88.3 0.947 Contact Map
3fovA 2 0.3833 81.5 0.952 Contact Map
1hh1A 2 0.4167 76.5 0.954 Contact Map
4qbnA 2 0.3875 71.6 0.956 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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