Q9HSF0 - HALSA - GREMLIN Contacts

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Q9HSF0 - Uncharacterized protein

UniProt:	Q9HSF0	Sequences: MGLFDRLRGSDAGRVAFVGIDGVPFSLIRDHPDTFPNLHDAMAAGSGGAIESIVPPESSACWPSLTTGVNPGETGVYGFQDRETGSYETYVPMGQDVQATRVWDRVTDAGRDATVMNVPVTFPPQRNVQRMSSGFLSPDIEKAAHPPEFADALEATDYRIDVNAKLGHDDDKAAFIEDAHETIDARQEAFLEYIDADDWDLFFGVFMSTDRVNHFLFKDYEEHGDNEAAFFEFYEKVDRYIGEIRAAIADDVTLVVASDHGFTSEDYEVHLNSWLADQGWLSFDSPDPEGLGDISDDTQVYSFIPGRFYLNREGREPRGSVAADDADAVLAEVSDALTALESPDGRQVVDRVVEGDAVFDGDHDAIAPDLVAIPNHGFDLKAGFSTPGDVFRHGPRNGMHSFENATLVTDAPDVSIPSSTNLYDIAPTLLDLMDVQYDSREFDGDTLVTADD	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	452 (364)
Sequences:	3098 (2370)
Seq/√Len:	124.2

HALSA - Q9HSF0
SULSO - Q980U2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
37_N	242_G	7.370	1.00
64_S	74_T	4.381	1.00
100_T	108_D	3.335	1.00
73_E	443_D	3.329	1.00
15_V	429_L	3.072	1.00
130_R	133_S	2.893	1.00
334_S	370_L	2.825	1.00
227_E	230_F	2.805	1.00
334_S	338_T	2.630	1.00
253_T	413_D	2.625	1.00
39_H	43_A	2.586	1.00
187_Q	206_F	2.572	1.00
241_I	245_R	2.555	1.00
106_V	111_R	2.497	1.00
100_T	105_R	2.474	1.00
191_L	243_E	2.457	1.00
190_F	204_G	2.425	1.00
255_V	429_L	2.360	1.00
16_A	252_V	2.338	1.00
127_N	133_S	2.333	1.00
191_L	247_A	2.244	1.00
129_Q	133_S	2.217	1.00
188_E	243_E	2.201	1.00
63_P	97_V	2.186	1.00
107_T	133_S	2.151	1.00
19_G	62_W	2.123	1.00
127_N	134_G	2.121	1.00
40_D	44_A	2.079	1.00
354_E	357_A	2.036	1.00
16_A	254_L	2.034	1.00
128_V	131_M	2.017	1.00
352_V	370_L	2.017	1.00
44_A	410_D	2.014	1.00
128_V	134_G	2.000	1.00
40_D	249_A	1.962	1.00
14_R	200_D	1.954	1.00
424_D	444_G	1.940	1.00
210_D	214_H	1.920	1.00
111_R	200_D	1.916	1.00
184_D	188_E	1.896	1.00
15_V	253_T	1.894	1.00
127_N	130_R	1.879	1.00
128_V	133_S	1.863	1.00
38_L	256_V	1.839	1.00
53_I	64_S	1.826	1.00
310_L	313_E	1.823	1.00
66_T	203_F	1.812	1.00
35_F	39_H	1.805	1.00
245_R	254_L	1.793	1.00
406_T	409_T	1.787	1.00
38_L	241_I	1.784	1.00
127_N	131_M	1.783	1.00
69_V	73_E	1.756	1.00
109_A	436_Q	1.740	1.00
374_P	380_L	1.728	1.00
41_A	408_V	1.720	1.00
329_V	332_E	1.719	1.00
131_M	134_G	1.703	1.00
146_P	149_F	1.695	1.00
103_W	201_L	1.688	1.00
22_G	262_F	1.681	1.00
18_V	254_L	1.676	1.00
258_S	405_A	1.655	1.00
17_F	203_F	1.630	1.00
106_V	113_A	1.623	1.00
327_D	331_A	1.606	1.00
17_F	257_A	1.583	1.00
69_V	99_A	1.580	1.00
34_T	235_E	1.569	1.00
333_V	336_A	1.559	1.00
214_H	259_D	1.546	1.00
64_S	422_L	1.543	1.00
70_N	423_Y	1.528	1.00
213_N	262_F	1.522	1.00
253_T	412_P	1.505	1.00
127_N	132_S	1.493	1.00
58_S	62_W	1.485	1.00
248_I	254_L	1.480	1.00
242_G	246_A	1.472	1.00
187_Q	244_I	1.471	1.00
132_S	135_F	1.465	1.00
37_N	40_D	1.460	1.00
65_L	257_A	1.448	1.00
330_L	334_S	1.444	1.00
50_I	425_I	1.440	1.00
61_C	422_L	1.413	1.00
52_S	61_C	1.411	1.00
332_E	335_D	1.404	1.00
184_D	240_Y	1.388	1.00
209_T	212_V	1.385	1.00
17_F	201_L	1.384	1.00
130_R	134_G	1.378	1.00
92_P	95_Q	1.370	1.00
82_R	87_Y	1.370	1.00
300_V	303_F	1.366	1.00
214_H	260_H	1.362	1.00
425_I	429_L	1.359	1.00
111_R	433_M	1.358	1.00
253_T	432_L	1.353	1.00
411_A	414_V	1.352	1.00
62_W	79_F	1.349	1.00
183_I	186_R	1.346	1.00
334_S	354_E	1.341	1.00
437_Y	440_R	1.339	1.00
210_D	259_D	1.337	1.00
228_A	232_E	1.335	1.00
40_D	245_R	1.323	1.00
263_T	402_F	1.318	1.00
101_R	104_D	1.316	1.00
65_L	425_I	1.309	1.00
48_G	420_T	1.309	1.00
335_D	338_T	1.302	1.00
309_Y	368_P	1.290	1.00
210_D	394_G	1.287	1.00
186_R	190_F	1.275	1.00
118_V	121_T	1.274	1.00
104_D	125_Q	1.269	1.00
187_Q	191_L	1.241	0.99
29_R	47_G	1.235	0.99
299_Q	302_S	1.232	0.99
36_P	238_D	1.231	0.99
129_Q	134_G	1.228	0.99
212_V	233_F	1.223	0.99
396_R	401_S	1.217	0.99
54_V	373_I	1.215	0.99
30_D	231_F	1.215	0.99
51_E	421_N	1.212	0.99
232_E	236_K	1.211	0.99
224_G	227_E	1.205	0.99
255_V	409_T	1.202	0.99
14_R	198_D	1.194	0.99
21_D	259_D	1.193	0.99
298_T	301_Y	1.189	0.99
58_S	259_D	1.182	0.99
46_S	414_V	1.181	0.99
42_M	47_G	1.178	0.99
50_I	405_A	1.176	0.99
240_Y	243_E	1.173	0.99
272_N	275_L	1.169	0.99
20_I	241_I	1.168	0.99
232_E	235_E	1.162	0.99
112_D	197_D	1.157	0.99
114_T	199_W	1.156	0.99
336_A	339_A	1.155	0.99
35_F	234_Y	1.155	0.99
102_V	429_L	1.142	0.99
129_Q	132_S	1.141	0.99
21_D	214_H	1.141	0.99
79_F	394_G	1.138	0.99
188_E	191_L	1.133	0.99
214_H	394_G	1.132	0.99
275_L	280_W	1.127	0.99
269_V	275_L	1.124	0.99
307_R	368_P	1.120	0.99
58_S	260_H	1.119	0.99
37_N	245_R	1.118	0.99
407_L	420_T	1.115	0.99
182_T	186_R	1.106	0.99
48_G	407_L	1.105	0.99
326_A	330_L	1.103	0.99
421_N	424_D	1.102	0.99
332_E	336_A	1.092	0.99
16_A	202_F	1.092	0.99
270_H	273_S	1.089	0.99
312_R	326_A	1.087	0.99
330_L	354_E	1.085	0.99
14_R	252_V	1.081	0.99
192_E	196_A	1.078	0.99
253_T	414_V	1.076	0.99
41_A	245_R	1.071	0.98
420_T	424_D	1.069	0.98
106_V	433_M	1.067	0.98
183_I	194_I	1.066	0.98
331_A	335_D	1.062	0.98
119_P	125_Q	1.061	0.98
355_G	358_V	1.057	0.98
350_D	375_N	1.046	0.98
70_N	443_D	1.032	0.98
114_T	118_V	1.026	0.98
271_L	372_A	1.025	0.98
330_L	370_L	1.024	0.98
376_H	402_F	1.023	0.98
204_G	244_I	1.023	0.98
36_P	242_G	1.017	0.98
41_A	256_V	1.017	0.98
349_V	372_A	1.011	0.98
208_S	212_V	1.007	0.98
97_V	101_R	0.999	0.98
248_I	252_V	0.995	0.98
131_M	135_F	0.994	0.98
268_E	271_L	0.992	0.98
20_I	209_T	0.991	0.98
376_H	400_H	0.988	0.97
15_V	433_M	0.984	0.97
56_P	395_P	0.979	0.97
55_P	71_P	0.978	0.97
13_G	413_D	0.975	0.97
53_I	71_P	0.971	0.97
206_F	244_I	0.968	0.97
14_R	251_D	0.967	0.97
354_E	358_V	0.963	0.97
305_P	308_F	0.962	0.97
119_P	122_F	0.958	0.97
342_S	349_V	0.955	0.97
305_P	312_R	0.954	0.97
83_E	86_S	0.948	0.97
128_V	132_S	0.940	0.97
276_A	279_G	0.940	0.97
105_R	108_D	0.939	0.97
284_D	287_D	0.936	0.96
18_V	241_I	0.936	0.96
213_N	230_F	0.934	0.96
238_D	242_G	0.931	0.96
34_T	231_F	0.930	0.96
58_S	210_D	0.927	0.96
189_A	192_E	0.926	0.96
49_A	418_S	0.925	0.96
273_S	277_D	0.920	0.96
40_D	410_D	0.919	0.96
23_V	234_Y	0.918	0.96
334_S	352_V	0.918	0.96
298_T	303_F	0.917	0.96
58_S	79_F	0.915	0.96
106_V	201_L	0.915	0.96
182_T	185_A	0.914	0.96
226_N	264_S	0.912	0.96
119_P	123_P	0.912	0.96
128_V	135_F	0.909	0.96
209_T	237_V	0.908	0.96
184_D	243_E	0.907	0.96
84_T	369_D	0.899	0.96
52_S	56_P	0.896	0.95
339_A	415_S	0.895	0.95
40_D	43_A	0.895	0.95
262_F	392_R	0.891	0.95
337_L	372_A	0.888	0.95
351_R	375_N	0.887	0.95
13_G	251_D	0.887	0.95
136_L	139_D	0.883	0.95
41_A	410_D	0.882	0.95
225_D	264_S	0.881	0.95
276_A	280_W	0.880	0.95
299_Q	303_F	0.878	0.95
79_F	395_P	0.877	0.95
352_V	372_A	0.872	0.95
84_T	309_Y	0.871	0.95
216_L	229_A	0.870	0.95
121_T	125_Q	0.868	0.95
298_T	302_S	0.868	0.95
267_Y	378_F	0.867	0.95
192_E	195_D	0.866	0.95
100_T	104_D	0.862	0.94
22_G	213_N	0.861	0.94
27_L	213_N	0.860	0.94
69_V	443_D	0.850	0.94
58_S	214_H	0.847	0.94
144_A	147_P	0.847	0.94
70_N	361_G	0.841	0.94
23_V	27_L	0.839	0.94
62_W	214_H	0.831	0.93
183_I	189_A	0.830	0.93
35_F	38_L	0.828	0.93
103_W	115_V	0.827	0.93
199_W	202_F	0.827	0.93
115_V	121_T	0.824	0.93
117_N	121_T	0.824	0.93
308_F	311_N	0.822	0.93
37_N	241_I	0.822	0.93
398_G	401_S	0.818	0.93
21_D	260_H	0.816	0.93
331_A	354_E	0.815	0.93
132_S	138_P	0.815	0.93
255_V	432_L	0.811	0.92
409_T	414_V	0.810	0.92
270_H	275_L	0.806	0.92
20_I	23_V	0.804	0.92
118_V	122_F	0.802	0.92
329_V	337_L	0.798	0.92
187_Q	194_I	0.795	0.92
134_G	138_P	0.795	0.92
330_L	369_D	0.794	0.92
83_E	366_I	0.793	0.92
79_F	210_D	0.783	0.91
275_L	279_G	0.780	0.91
266_D	377_G	0.779	0.91
277_D	283_F	0.778	0.91
116_M	190_F	0.776	0.91
284_D	288_P	0.776	0.91
118_V	125_Q	0.770	0.90
265_E	393_H	0.769	0.90
351_R	357_A	0.769	0.90
426_A	430_L	0.769	0.90
112_D	200_D	0.768	0.90
257_A	405_A	0.765	0.90
20_I	256_V	0.764	0.90
135_F	138_P	0.764	0.90
330_L	356_D	0.764	0.90
50_I	420_T	0.764	0.90
23_V	406_T	0.763	0.90
121_T	124_P	0.762	0.90
149_F	154_E	0.761	0.90
413_D	432_L	0.760	0.90
54_V	397_N	0.760	0.90
17_F	255_V	0.756	0.90
107_T	113_A	0.754	0.89
336_A	340_L	0.752	0.89
274_W	340_L	0.751	0.89
271_L	275_L	0.746	0.89
150_A	154_E	0.745	0.89
274_W	278_Q	0.743	0.89
263_T	376_H	0.742	0.89
62_W	205_V	0.742	0.89
20_I	406_T	0.741	0.89
231_F	235_E	0.739	0.88
268_E	273_S	0.738	0.88
271_L	277_D	0.738	0.88
147_P	152_A	0.738	0.88
62_W	210_D	0.738	0.88
334_S	340_L	0.738	0.88
122_F	125_Q	0.737	0.88
66_T	201_L	0.726	0.88
145_H	148_E	0.721	0.87
186_R	189_A	0.719	0.87
52_S	422_L	0.719	0.87
130_R	135_F	0.719	0.87
195_D	247_A	0.717	0.87
33_D	36_P	0.717	0.87
305_P	311_N	0.714	0.87
271_L	276_A	0.713	0.87
17_F	65_L	0.713	0.87
310_L	329_V	0.713	0.87
46_S	416_I	0.711	0.86
430_L	437_Y	0.710	0.86
268_E	272_N	0.705	0.86
135_F	139_D	0.704	0.86
282_S	287_D	0.703	0.86
191_L	194_I	0.701	0.86
145_H	150_A	0.701	0.86
257_A	406_T	0.700	0.86
275_L	281_L	0.699	0.86
304_I	308_F	0.698	0.85
18_V	244_I	0.698	0.85
189_A	193_Y	0.696	0.85
414_V	428_T	0.696	0.85
309_Y	312_R	0.694	0.85
427_P	443_D	0.692	0.85
113_A	118_V	0.692	0.85
332_E	339_A	0.692	0.85
70_N	421_N	0.691	0.85
19_G	205_V	0.691	0.85
182_T	189_A	0.690	0.85
310_L	330_L	0.689	0.85
313_E	328_A	0.686	0.84
335_D	339_A	0.685	0.84
244_I	247_A	0.684	0.84
188_E	192_E	0.683	0.84
274_W	280_W	0.683	0.84
66_T	102_V	0.680	0.84
243_E	246_A	0.678	0.84
105_R	109_A	0.677	0.84
307_R	312_R	0.677	0.84
280_W	333_V	0.675	0.84
20_I	237_V	0.674	0.83
63_P	99_A	0.673	0.83
235_E	239_R	0.668	0.83
53_I	61_C	0.667	0.83
272_N	279_G	0.666	0.83
79_F	214_H	0.664	0.83
257_A	407_L	0.664	0.83
249_A	252_V	0.664	0.83
62_W	394_G	0.663	0.82
362_D	367_A	0.660	0.82
36_P	246_A	0.658	0.82
258_S	261_G	0.656	0.82
115_V	125_Q	0.656	0.82
82_R	89_T	0.655	0.82
302_S	308_F	0.654	0.82
335_D	356_D	0.651	0.81
12_A	200_D	0.650	0.81
186_R	193_Y	0.649	0.81
120_V	123_P	0.649	0.81
102_V	430_L	0.648	0.81
103_W	119_P	0.647	0.81
107_T	131_M	0.647	0.81
427_P	444_G	0.646	0.81
420_T	428_T	0.643	0.80
245_R	249_A	0.643	0.80
279_G	284_D	0.642	0.80
210_D	260_H	0.642	0.80
72_G	363_H	0.642	0.80
361_G	367_A	0.641	0.80
195_D	243_E	0.640	0.80
225_D	230_F	0.639	0.80
59_S	62_W	0.639	0.80
72_G	361_G	0.636	0.80
106_V	435_V	0.636	0.80
33_D	39_H	0.635	0.80
116_M	193_Y	0.635	0.80
243_E	247_A	0.634	0.80
82_R	86_S	0.634	0.80
272_N	280_W	0.631	0.79
253_T	409_T	0.629	0.79
185_A	194_I	0.627	0.79
69_V	438_D	0.626	0.79
183_I	187_Q	0.624	0.78
42_M	271_L	0.624	0.78
40_D	246_A	0.621	0.78
44_A	335_D	0.619	0.78
269_V	272_N	0.619	0.78
184_D	236_K	0.618	0.78
337_L	340_L	0.618	0.78
56_P	396_R	0.617	0.78
327_D	332_E	0.617	0.78
306_G	312_R	0.616	0.78
334_S	337_L	0.613	0.77
377_G	380_L	0.613	0.77
428_T	444_G	0.612	0.77
145_H	154_E	0.612	0.77
430_L	440_R	0.608	0.77
190_F	202_F	0.608	0.77
96_D	99_A	0.605	0.76
41_A	254_L	0.604	0.76
194_I	247_A	0.604	0.76
101_R	105_R	0.604	0.76
185_A	189_A	0.603	0.76
269_V	277_D	0.601	0.76
281_L	284_D	0.601	0.76
333_V	337_L	0.601	0.76
239_R	242_G	0.601	0.76
24_P	27_L	0.601	0.76
349_V	352_V	0.599	0.76
28_I	408_V	0.599	0.76
117_N	120_V	0.598	0.76
337_L	370_L	0.598	0.76
75_G	366_I	0.598	0.76
152_A	155_A	0.597	0.75
208_S	233_F	0.596	0.75
34_T	38_L	0.595	0.75
108_D	360_D	0.594	0.75
83_E	90_Y	0.594	0.75
405_A	409_T	0.592	0.75
55_P	395_P	0.591	0.75
274_W	277_D	0.589	0.74
110_G	114_T	0.588	0.74
282_S	286_P	0.587	0.74
59_S	79_F	0.586	0.74
44_A	416_I	0.586	0.74
30_D	226_N	0.586	0.74
285_S	288_P	0.584	0.74
118_V	124_P	0.583	0.74
57_E	78_G	0.581	0.73
115_V	203_F	0.580	0.73
396_R	400_H	0.580	0.73
30_D	33_D	0.580	0.73
207_M	211_R	0.579	0.73
304_I	307_R	0.579	0.73
55_P	361_G	0.576	0.73
259_D	394_G	0.576	0.73
414_V	432_L	0.576	0.73
353_V	358_V	0.574	0.73
423_Y	443_D	0.572	0.72
406_T	410_D	0.572	0.72
115_V	120_V	0.571	0.72
132_S	137_S	0.568	0.72
38_L	42_M	0.567	0.72
353_V	373_I	0.566	0.71
76_V	97_V	0.565	0.71
67_T	101_R	0.565	0.71
134_G	139_D	0.562	0.71
41_A	241_I	0.561	0.71
56_P	62_W	0.560	0.71
337_L	349_V	0.554	0.70
62_W	260_H	0.553	0.70
281_L	287_D	0.552	0.70
55_P	58_S	0.551	0.69
187_Q	193_Y	0.551	0.69
439_S	443_D	0.550	0.69
237_V	256_V	0.549	0.69
306_G	309_Y	0.549	0.69
205_V	394_G	0.548	0.69
148_E	152_A	0.548	0.69
190_F	370_L	0.545	0.69
50_I	53_I	0.545	0.69
25_F	402_F	0.545	0.69
334_S	357_A	0.545	0.69
236_K	239_R	0.545	0.69
146_P	154_E	0.544	0.68
310_L	326_A	0.544	0.68
29_R	33_D	0.544	0.68
328_A	332_E	0.543	0.68
355_G	371_V	0.542	0.68
129_Q	135_F	0.542	0.68
275_L	278_Q	0.541	0.68
79_F	259_D	0.541	0.68
205_V	210_D	0.540	0.68
70_N	360_D	0.539	0.68
192_E	197_D	0.539	0.68
150_A	153_L	0.536	0.67
146_P	152_A	0.533	0.67
23_V	209_T	0.533	0.67
254_L	258_S	0.531	0.67
76_V	90_Y	0.530	0.67
114_T	121_T	0.529	0.66
326_A	329_V	0.527	0.66
331_A	334_S	0.527	0.66
145_H	151_D	0.525	0.66
337_L	341_E	0.524	0.66
276_A	282_S	0.523	0.65
120_V	125_Q	0.523	0.65
250_D	410_D	0.522	0.65
276_A	281_L	0.521	0.65
145_H	149_F	0.520	0.65
350_D	353_V	0.520	0.65
279_G	288_P	0.520	0.65
273_S	276_A	0.520	0.65
281_L	285_S	0.519	0.65
187_Q	243_E	0.519	0.65
64_S	283_F	0.518	0.65
12_A	111_R	0.518	0.65
69_V	440_R	0.517	0.65
119_P	205_V	0.517	0.65
146_P	153_L	0.516	0.64
132_S	139_D	0.515	0.64
272_N	277_D	0.514	0.64
115_V	119_P	0.514	0.64
229_A	232_E	0.514	0.64
253_T	411_A	0.513	0.64
127_N	135_F	0.513	0.64
246_A	331_A	0.513	0.64
33_D	234_Y	0.512	0.64
190_F	244_I	0.511	0.64
306_G	310_L	0.509	0.63
98_Q	364_D	0.509	0.63
84_T	271_L	0.508	0.63
21_D	62_W	0.506	0.63
326_A	333_V	0.505	0.63
51_E	419_S	0.505	0.63
353_V	357_A	0.504	0.63
305_P	309_Y	0.504	0.63
114_T	125_Q	0.504	0.63
308_F	312_R	0.504	0.63
66_T	115_V	0.503	0.63
133_S	139_D	0.503	0.63
92_P	258_S	0.502	0.62
391_F	394_G	0.501	0.62
276_A	283_F	0.500	0.62

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3q3qA	2	0.9204	100	0.431	Contact Map
2gsoA	1	0.7788	100	0.466	Contact Map
3nkqA	1	0.7743	100	0.469	Contact Map
4lr2A	1	0.7721	100	0.475	Contact Map
1ei6A	2	0.8341	100	0.477	Contact Map
3szyA	1	0.8429	100	0.477	Contact Map
4gtwA	1	0.8296	100	0.486	Contact Map
3b5qA	1	0.6195	100	0.663	Contact Map
4fdiA	2	0.7013	100	0.665	Contact Map
4upkA	3	0.6659	100	0.666	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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