GREMLIN Database
TF2B2 - Transcription initiation factor IIB 2
UniProt: Q9HRE6 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 325 (280)
Sequences: 1079 (685)
Seq/√Len: 40.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
244_A283_I6.5681.00
150_A189_L4.1141.00
50_C53_C3.8511.00
250_P292_H3.7641.00
32_C53_C3.7621.00
39_V48_S3.7101.00
313_R316_E3.7081.00
256_R290_T3.6201.00
296_Q311_R3.3951.00
39_V57_V3.3081.00
149_M189_L2.8991.00
142_A167_Y2.8351.00
145_E148_R2.7891.00
32_C35_C2.7471.00
189_L205_V2.6991.00
35_C50_C2.6131.00
297_S307_E2.5651.00
150_A160_R2.5541.00
143_L164_S2.5321.00
159_V192_S2.3361.00
32_C50_C2.3021.00
42_D45_H2.2691.00
280_A303_T2.2461.00
307_E315_Q2.1860.99
35_C53_C2.1390.99
163_A192_S2.1350.99
256_R259_E2.1210.99
167_Y176_L2.0280.99
178_G275_P2.0190.99
213_E217_K1.9980.99
70_A73_S1.9640.99
155_L189_L1.9310.99
261_I278_L1.9290.99
149_M208_V1.9120.99
257_S279_A1.9030.99
257_S286_S1.8970.99
38_Q52_D1.8780.98
49_V302_V1.8340.98
260_L286_S1.8140.98
200_R204_E1.7590.98
162_T195_Q1.7540.98
165_V168_R1.7450.97
70_A75_E1.6640.97
68_W79_K1.6590.97
224_V231_V1.6520.96
142_A171_L1.6350.96
142_A218_R1.6170.96
249_L294_V1.5770.95
205_V208_V1.5640.95
277_G309_T1.5580.95
243_F283_I1.5530.95
68_W71_F1.5490.95
253_V290_T1.5240.94
38_Q51_V1.5200.94
91_H94_G1.5200.94
162_T196_L1.5190.94
194_R217_K1.5160.94
67_E81_R1.5150.94
260_L282_A1.5050.94
298_E302_V1.5020.94
102_Q126_N1.5020.94
243_F247_L1.5000.94
42_D46_G1.4980.94
295_T298_E1.4970.94
297_S310_I1.4890.94
65_G68_W1.4840.94
293_K298_E1.4300.92
287_S302_V1.4040.92
169_R173_E1.3770.91
277_G281_A1.3620.90
261_I282_A1.3530.90
225_R298_E1.3380.89
237_A262_S1.3180.89
253_V292_H1.3160.89
305_V310_I1.3110.88
191_A229_L1.3070.88
146_I164_S1.2870.87
37_G52_D1.2790.87
104_K108_G1.2620.86
142_A146_I1.2610.86
235_D238_Q1.2560.86
32_C36_G1.2550.86
48_S260_L1.2490.86
280_A285_A1.2490.86
235_D261_I1.2470.86
204_E242_R1.2450.86
296_Q308_V1.2450.86
129_F186_T1.2360.85
50_C54_G1.2350.85
282_A286_S1.2350.85
191_A194_R1.2320.85
91_H311_R1.2220.85
52_D150_A1.2170.84
146_I185_A1.2060.84
202_I217_K1.2000.83
254_E258_R1.1800.82
142_A310_I1.1740.82
34_E55_L1.1720.82
34_E37_G1.1690.82
98_N126_N1.1610.81
153_L266_A1.1430.80
294_V298_E1.1340.80
69_R81_R1.1330.80
167_Y181_I1.1180.79
78_E83_G1.1140.79
63_D67_E1.1040.78
217_K243_F1.1020.78
164_S171_L1.1000.78
140_K144_G1.0980.78
205_V268_G1.0770.76
261_I279_A1.0760.76
268_G273_K1.0700.76
247_L283_I1.0630.75
280_A310_I1.0580.75
83_G168_R1.0560.75
81_R97_T1.0550.75
139_L164_S1.0530.75
184_V223_I1.0480.74
235_D265_Q1.0470.74
300_S303_T1.0470.74
131_T146_I1.0370.73
68_W85_P1.0330.73
201_S204_E1.0260.73
190_Y234_A1.0240.72
252_E290_T1.0220.72
150_A278_L1.0170.72
263_N267_D1.0150.72
294_V307_E1.0110.71
281_A309_T1.0090.71
162_T165_V1.0030.71
48_S51_V1.0010.71
256_R289_L1.0010.71
202_I213_E0.9750.69
87_T102_Q0.9700.68
283_I303_T0.9680.68
32_C37_G0.9650.68
41_D58_E0.9640.68
138_N191_A0.9640.68
41_D47_E0.9610.68
41_D48_S0.9570.67
201_S205_V0.9500.67
269_V287_S0.9480.66
280_A300_S0.9460.66
217_K220_Y0.9410.66
146_I152_A0.9410.66
71_F243_F0.9310.65
33_P57_V0.9280.65
31_T51_V0.9270.65
92_D296_Q0.9260.65
36_G40_I0.9230.64
279_A303_T0.9210.64
41_D260_L0.9200.64
62_I68_W0.9190.64
220_Y223_I0.9160.64
40_I51_V0.9070.63
291_N295_T0.9010.62
186_T193_A0.8940.62
49_V315_Q0.8900.62
39_V44_E0.8900.62
308_V311_R0.8890.61
31_T36_G0.8880.61
191_A287_S0.8820.61
68_W81_R0.8790.61
209_S314_Y0.8780.60
239_Y244_A0.8770.60
146_I163_A0.8760.60
277_G313_R0.8750.60
56_V88_N0.8660.59
164_S168_R0.8660.59
283_I299_V0.8650.59
134_S187_A0.8650.59
135_K248_D0.8620.59
166_I188_A0.8610.59
249_L253_V0.8570.59
214_M315_Q0.8560.59
100_G148_R0.8550.58
173_E298_E0.8480.58
145_E218_R0.8440.57
55_L95_L0.8430.57
142_A281_A0.8410.57
155_L319_E0.8350.57
87_T91_H0.8270.56
217_K274_S0.8230.56
184_V219_T0.8230.56
200_R242_R0.8210.55
284_Y288_L0.8170.55
215_E218_R0.8100.54
108_G202_I0.8080.54
277_G305_V0.8080.54
159_V198_T0.8070.54
237_A265_Q0.8050.54
67_E79_K0.8030.54
136_E173_E0.8030.54
67_E71_F0.7960.53
57_V274_S0.7960.53
242_R274_S0.7890.52
155_L193_A0.7870.52
36_G53_C0.7850.52
36_G232_A0.7840.52
212_D215_E0.7840.52
92_D95_L0.7830.52
172_S297_S0.7790.51
177_P229_L0.7780.51
41_D49_V0.7770.51
274_S310_I0.7770.51
249_L292_H0.7710.51
163_A188_A0.7700.51
168_R192_S0.7690.51
52_D156_P0.7620.50
125_W167_Y0.7610.50
264_A320_A0.7610.50
121_R125_W0.7580.49
40_I280_A0.7570.49
264_A270_T0.7550.49
76_K84_A0.7530.49
234_A276_V0.7520.49
206_A211_I0.7510.49
289_L314_Y0.7440.48
137_R145_E0.7440.48
128_R272_G0.7390.48
193_A261_I0.7360.47
46_G61_G0.7350.47
72_N278_L0.7350.47
190_Y193_A0.7330.47
297_S312_N0.7330.47
180_S221_R0.7330.47
274_S284_Y0.7250.46
134_S316_E0.7220.46
212_D216_F0.7210.46
216_F262_S0.7210.46
287_S293_K0.7200.46
260_L272_G0.7190.46
160_R189_L0.7160.46
210_R280_A0.7150.46
85_P295_T0.7100.45
139_L199_P0.7100.45
170_A229_L0.7090.45
136_E160_R0.7060.45
77_D86_T0.7000.44
142_A180_S0.6990.44
240_V261_I0.6990.44
178_G309_T0.6990.44
41_D108_G0.6980.44
59_E64_R0.6970.44
232_A244_A0.6960.44
205_V231_V0.6950.44
134_S222_Y0.6930.44
270_T280_A0.6930.44
138_N167_Y0.6930.44
237_A304_D0.6850.43
67_E83_G0.6850.43
142_A170_A0.6850.43
166_I192_S0.6820.43
244_A247_L0.6810.42
146_I167_Y0.6800.42
189_L268_G0.6800.42
40_I156_P0.6750.42
159_V265_Q0.6750.42
73_S78_E0.6740.42
220_Y277_G0.6740.42
145_E149_M0.6720.42
273_K278_L0.6720.42
55_L245_S0.6710.42
125_W128_R0.6700.41
199_P238_Q0.6670.41
293_K302_V0.6670.41
102_Q136_E0.6660.41
297_S305_V0.6610.41
32_C39_V0.6610.41
273_K286_S0.6570.40
185_A230_E0.6570.40
167_Y313_R0.6510.40
158_E245_S0.6490.40
141_Q265_Q0.6480.39
305_V309_T0.6470.39
139_L143_L0.6470.39
280_A284_Y0.6460.39
180_S275_P0.6430.39
269_V316_E0.6430.39
186_T209_S0.6420.39
70_A78_E0.6410.39
127_E140_K0.6410.39
56_V188_A0.6410.39
93_K175_L0.6410.39
168_R255_R0.6390.39
177_P278_L0.6370.38
67_E70_A0.6350.38
285_A299_V0.6340.38
177_P307_E0.6330.38
72_N75_E0.6300.38
207_N304_D0.6300.38
190_Y231_V0.6300.38
91_H161_E0.6290.38
216_F234_A0.6280.38
129_F239_Y0.6270.38
278_L313_R0.6270.38
162_T197_S0.6270.38
153_L264_A0.6260.38
287_S292_H0.6230.37
65_G70_A0.6230.37
70_A313_R0.6200.37
96_S187_A0.6170.37
285_A315_Q0.6170.37
170_A303_T0.6160.37
225_R245_S0.6150.37
298_E301_E0.6130.36
100_G283_I0.6120.36
205_V299_V0.6110.36
269_V280_A0.6100.36
58_E302_V0.6100.36
153_L208_V0.6100.36
219_T310_I0.6070.36
95_L99_I0.6060.36
74_T319_E0.6020.35
161_E164_S0.6010.35
138_N148_R0.6010.35
63_D69_R0.6000.35
173_E176_L0.6000.35
63_D97_T0.5990.35
193_A234_A0.5990.35
143_L161_E0.5990.35
199_P282_A0.5980.35
74_T284_Y0.5980.35
251_D255_R0.5960.35
155_L166_I0.5960.35
253_V257_S0.5950.35
71_F247_L0.5950.35
39_V46_G0.5940.35
159_V196_L0.5930.35
120_Q124_K0.5910.35
60_N66_P0.5910.35
39_V150_A0.5910.35
45_H311_R0.5910.35
82_V211_I0.5900.34
66_P284_Y0.5900.34
198_T225_R0.5890.34
78_E270_T0.5880.34
253_V282_A0.5880.34
223_I276_V0.5880.34
175_L223_I0.5880.34
54_G272_G0.5860.34
223_I246_D0.5830.34
240_V312_N0.5800.34
160_R266_A0.5780.33
161_E174_D0.5770.33
100_G103_D0.5770.33
209_S234_A0.5760.33
48_S61_G0.5750.33
37_G53_C0.5730.33
97_T200_R0.5710.33
167_Y170_A0.5660.32
40_I48_S0.5660.32
82_V205_V0.5640.32
95_L98_N0.5630.32
92_D311_R0.5630.32
57_V215_E0.5620.32
30_L262_S0.5610.32
295_T311_R0.5590.32
170_A175_L0.5590.32
99_I137_R0.5580.32
295_T307_E0.5580.32
71_F83_G0.5570.32
32_C55_L0.5570.32
108_G278_L0.5520.31
205_V215_E0.5510.31
128_R179_R0.5510.31
211_I246_D0.5510.31
205_V241_P0.5480.31
56_V126_N0.5470.31
49_V67_E0.5470.31
143_L185_A0.5460.31
69_R79_K0.5460.31
38_Q58_E0.5460.31
34_E52_D0.5460.31
257_S261_I0.5450.31
146_I225_R0.5450.31
140_K261_I0.5430.31
46_G155_L0.5410.30
80_S83_G0.5410.30
89_M92_D0.5390.30
217_K291_N0.5390.30
55_L63_D0.5380.30
34_E311_R0.5370.30
142_A179_R0.5350.30
87_T90_M0.5340.30
163_A269_V0.5340.30
133_N181_I0.5340.30
182_E314_Y0.5340.30
274_S277_G0.5330.30
182_E185_A0.5330.30
142_A306_S0.5320.30
97_T254_E0.5310.30
121_R124_K0.5300.30
197_S233_P0.5300.30
45_H61_G0.5280.29
314_Y318_L0.5280.29
302_V310_I0.5280.29
86_T110_S0.5260.29
170_A236_P0.5230.29
70_A79_K0.5230.29
220_Y272_G0.5210.29
167_Y230_E0.5200.29
207_N259_E0.5200.29
51_V262_S0.5200.29
73_S306_S0.5170.29
180_S311_R0.5150.28
31_T37_G0.5130.28
221_R238_Q0.5120.28
252_E318_L0.5070.28
91_H164_S0.5060.28
164_S218_R0.5060.28
58_E64_R0.5050.28
100_G231_V0.5050.28
146_I161_E0.5040.28
253_V272_G0.5040.28
160_R299_V0.5020.27
204_E215_E0.5020.27
87_T178_G0.5010.27
60_N299_V0.5000.27
154_G229_L0.5000.27
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4bbrM 1 0.5785 100 0.265 Contact Map
1aisB 1 0.5785 100 0.599 Contact Map
1c9bA 1 0.5877 100 0.607 Contact Map
2pk2A 3 0.6646 99.9 0.714 Contact Map
4f6uB 1 0.6985 99.9 0.72 Contact Map
2w96A 1 0.6985 99.9 0.728 Contact Map
2b9rA 1 0.6492 99.9 0.732 Contact Map
4eojB 1 0.6585 99.9 0.736 Contact Map
3g33B 2 0.6646 99.9 0.737 Contact Map
2ivxA 1 0.6615 99.9 0.74 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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