GREMLIN Database
Q9HQZ1 - GufA protein
UniProt: Q9HQZ1 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 266 (233)
Sequences: 5459 (3680)
Seq/√Len: 241.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
81_L216_F4.1691.00
62_A215_G3.5511.00
41_G251_G3.3321.00
119_F195_G3.3151.00
14_G196_A3.0061.00
22_G186_V2.8721.00
59_F75_A2.8191.00
45_L258_G2.7111.00
123_V199_A2.6321.00
255_G259_M2.6001.00
75_A222_V2.5451.00
79_V253_A2.5061.00
95_E98_D2.4341.00
74_G212_F2.4261.00
183_G187_F2.3181.00
177_R180_T2.2651.00
99_I103_D2.2601.00
94_F97_R2.1811.00
251_G255_G2.1581.00
10_V14_G2.0381.00
91_D94_F2.0281.00
255_G258_G2.0271.00
96_P99_I1.9771.00
179_W183_G1.9291.00
99_I102_A1.8441.00
50_M221_M1.7391.00
52_S260_V1.7201.00
52_S56_F1.6851.00
41_G247_E1.6661.00
47_S229_F1.6571.00
253_A257_A1.6551.00
100_A103_D1.6171.00
55_G222_V1.6171.00
97_R100_A1.5831.00
21_T24_G1.5501.00
83_V87_H1.4901.00
76_L257_A1.4881.00
198_I201_V1.4871.00
13_A129_F1.4781.00
72_A257_A1.4711.00
14_G18_D1.4691.00
79_V226_L1.4641.00
161_V221_M1.4581.00
15_L19_L1.4481.00
20_A157_S1.4411.00
257_A261_P1.4341.00
111_A184_V1.4251.00
12_F16_L1.4211.00
26_L182_V1.4091.00
70_E212_F1.3911.00
17_T24_G1.3781.00
73_A212_F1.3681.00
56_F260_V1.3651.00
51_V225_V1.3601.00
86_E223_F1.3541.00
208_A212_F1.3451.00
27_P161_V1.3441.00
121_E160_N1.3431.00
38_W42_L1.3421.00
115_T191_P1.3421.00
11_V15_L1.3391.00
123_V202_F1.3371.00
91_D103_D1.3331.00
20_A161_V1.3331.00
172_H178_R1.3271.00
74_G77_A1.3191.00
54_S222_V1.3161.00
97_R101_A1.3061.00
37_R247_E1.2971.00
24_G28_F1.2961.00
17_T20_A1.2921.00
117_H122_G1.2921.00
38_W41_G1.2901.00
23_L161_V1.2871.00
57_G259_M1.2841.00
117_H159_H1.2831.00
62_A71_I1.2801.00
74_G215_G1.2731.00
125_V156_I1.2701.00
22_G189_S1.2681.00
98_D102_A1.2651.00
43_W233_A1.2611.00
132_M146_P1.2591.00
61_E64_N1.2531.00
153_T157_S1.2521.00
94_F100_A1.2461.00
197_G201_V1.2391.00
217_A220_A1.2381.00
17_T21_T1.2351.00
132_M149_A1.2351.00
25_A168_S1.2301.00
61_E65_Y1.2181.00
21_T28_F1.1931.00
53_A57_G1.1911.00
26_L29_F1.1871.00
76_L80_A1.1811.00
16_L153_T1.1771.00
167_V188_T1.1761.00
98_D101_A1.1761.00
31_V34_I1.1711.00
150_V154_I1.1691.00
160_N221_M1.1691.00
10_V28_F1.1521.00
63_L71_I1.1441.00
96_P100_A1.1431.00
70_E77_A1.1361.00
118_S188_T1.1291.00
51_V252_I1.1281.00
45_L254_V1.1231.00
78_G82_V1.1221.00
160_N164_G1.1201.00
114_L121_E1.1191.00
164_G185_A1.1051.00
10_V17_T1.1051.00
111_A167_V1.1041.00
62_A211_P1.0991.00
73_A77_A1.0801.00
190_I194_I1.0751.00
74_G78_G1.0741.00
83_V223_F1.0581.00
200_Y203_V1.0571.00
163_E221_M1.0561.00
55_G59_F1.0501.00
226_L252_I1.0421.00
63_L72_A1.0401.00
94_F98_D1.0341.00
53_A159_H1.0291.00
171_L176_A1.0211.00
256_V260_V1.0151.00
114_L117_H1.0121.00
252_I255_G0.9951.00
128_S152_M0.9871.00
96_P101_A0.9851.00
237_G240_L0.9841.00
40_V233_A0.9841.00
14_G200_Y0.9821.00
230_I234_R0.9811.00
160_N188_T0.9791.00
123_V195_G0.9751.00
60_R64_N0.9691.00
95_E99_I0.9671.00
59_F218_G0.9591.00
152_M163_E0.9591.00
97_R102_A0.9521.00
252_I256_V0.9501.00
247_E251_G0.9491.00
115_T119_F0.9481.00
85_A90_D0.9341.00
36_D236_V0.9331.00
18_D193_P0.9311.00
157_S221_M0.9271.00
199_A202_F0.9171.00
257_A260_V0.9151.00
160_N163_E0.9091.00
111_A171_L0.9071.00
72_A261_P0.9031.00
22_G185_A0.9031.00
129_F149_A0.8941.00
55_G60_R0.8931.00
54_S57_G0.8921.00
40_V238_G0.8871.00
25_A185_A0.8780.99
84_V88_V0.8770.99
21_T25_A0.8650.99
70_E74_G0.8550.99
110_I113_V0.8460.99
12_F15_L0.8460.99
57_G159_H0.8460.99
254_V258_G0.8440.99
25_A28_F0.8390.99
54_S221_M0.8340.99
226_L230_I0.8320.99
47_S113_V0.8280.99
29_F182_V0.8230.99
78_G216_F0.8120.99
209_A212_F0.8100.99
95_E102_A0.8090.99
47_S230_I0.8090.99
160_N167_V0.8060.99
183_G186_V0.8000.99
132_M147_I0.8000.99
66_G71_I0.7970.99
49_I226_L0.7960.99
107_L174_H0.7890.99
160_N220_A0.7850.99
44_G233_A0.7840.99
180_T183_G0.7820.99
216_F220_A0.7810.99
25_A29_F0.7760.99
62_A218_G0.7740.99
15_L193_P0.7700.99
118_S193_P0.7700.99
163_E167_V0.7690.99
16_L20_A0.7610.98
125_V153_T0.7600.98
10_V196_A0.7580.98
164_G221_M0.7550.98
190_I193_P0.7540.98
119_F198_I0.7540.98
77_A212_F0.7530.98
112_G116_V0.7460.98
13_A17_T0.7390.98
95_E100_A0.7300.98
234_R248_L0.7280.98
132_M250_T0.7260.98
35_D38_W0.7240.98
166_A170_P0.7210.98
17_T57_G0.7200.98
48_G55_G0.7200.98
112_G151_F0.7190.98
181_L185_A0.7160.98
127_V131_D0.7160.98
11_V197_G0.7140.98
120_P163_E0.7130.98
77_A81_L0.7100.98
54_S225_V0.7100.98
53_A259_M0.7090.98
247_E255_G0.7080.98
19_L26_L0.7080.98
147_I151_F0.7070.98
236_V239_A0.6980.97
85_A116_V0.6950.97
95_E101_A0.6940.97
179_W254_V0.6940.97
49_I259_M0.6930.97
233_A248_L0.6900.97
156_I163_E0.6870.97
24_G164_G0.6840.97
61_E159_H0.6830.97
176_A180_T0.6790.97
18_D22_G0.6790.97
70_E73_A0.6790.97
91_D95_E0.6750.97
158_I168_S0.6740.97
51_V222_V0.6740.97
33_D36_D0.6720.97
80_A252_I0.6710.97
18_D28_F0.6690.97
14_G17_T0.6680.97
211_P219_G0.6610.96
69_T73_A0.6550.96
114_L167_V0.6540.96
43_W107_L0.6530.96
80_A84_V0.6520.96
180_T184_V0.6480.96
149_A153_T0.6480.96
85_A91_D0.6460.96
121_E163_E0.6440.96
71_I215_G0.6430.96
14_G197_G0.6430.96
65_Y190_I0.6400.96
10_V200_Y0.6390.96
47_S83_V0.6390.96
114_L164_G0.6390.96
45_L53_A0.6350.95
95_E103_D0.6320.95
122_G159_H0.6270.95
51_V55_G0.6260.95
167_V171_L0.6230.95
163_E188_T0.6230.95
29_F179_W0.6210.95
39_R42_L0.6200.95
124_A152_M0.6150.95
170_P228_E0.6140.94
37_R41_G0.6130.94
182_V186_V0.6120.94
17_T28_F0.6100.94
47_S252_I0.6080.94
168_S185_A0.6040.94
238_G247_E0.6030.94
114_L163_E0.6030.94
249_A252_I0.6020.94
157_S160_N0.6020.94
16_L157_S0.5990.94
186_V189_S0.5970.94
117_H121_E0.5970.94
50_M170_P0.5920.93
43_W46_A0.5900.93
53_A220_A0.5890.93
160_N192_Q0.5820.93
56_F61_E0.5810.93
10_V20_A0.5790.93
23_L26_L0.5770.92
111_A115_T0.5760.92
202_F205_I0.5760.92
117_H167_V0.5750.92
17_T22_G0.5750.92
124_A219_G0.5710.92
248_L251_G0.5710.92
13_A203_V0.5690.92
240_L250_T0.5690.92
108_A112_G0.5640.92
215_G219_G0.5640.92
19_L103_D0.5630.92
126_G217_A0.5610.91
82_V113_V0.5600.91
110_I229_F0.5580.91
187_F190_I0.5540.91
53_A152_M0.5530.91
249_A255_G0.5520.91
220_A224_L0.5510.91
9_F12_F0.5500.91
68_P260_V0.5500.91
187_F191_P0.5490.91
164_G167_V0.5480.90
220_A229_F0.5480.90
75_A215_G0.5460.90
225_V229_F0.5460.90
48_G217_A0.5460.90
230_I252_I0.5430.90
120_P124_A0.5430.90
15_L97_R0.5420.90
10_V13_A0.5400.90
197_G200_Y0.5400.90
107_L110_I0.5390.90
13_A196_A0.5390.90
18_D186_V0.5390.90
53_A217_A0.5390.90
18_D24_G0.5350.89
54_S75_A0.5350.89
55_G76_L0.5340.89
118_S164_G0.5320.89
88_V190_I0.5320.89
212_F216_F0.5290.89
171_L181_L0.5280.89
127_V203_V0.5250.89
20_A25_A0.5220.88
32_D37_R0.5210.88
163_E166_A0.5210.88
146_P150_V0.5210.88
180_T190_I0.5190.88
118_S160_N0.5180.88
192_Q221_M0.5180.88
14_G21_T0.5180.88
13_A153_T0.5170.88
12_F153_T0.5150.88
110_I167_V0.5140.87
78_G90_D0.5130.87
62_A65_Y0.5120.87
20_A24_G0.5100.87
98_D103_D0.5100.87
234_R237_G0.5080.87
12_F194_I0.5080.87
118_S192_Q0.5060.87
18_D160_N0.5050.87
57_G61_E0.5040.86
195_G200_Y0.5020.86
256_V259_M0.5010.86
42_L61_E0.5010.86
60_R63_L0.5000.86
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4dveA 3 0.4887 54.4 0.931 Contact Map
4g1uA 2 0.4737 33 0.94 Contact Map
4tkrA 2 0.4624 31.2 0.941 Contact Map
3rlbA 1 0.4323 23 0.944 Contact Map
4av3A 2 0.8083 22.2 0.945 Contact Map
4a01A 2 0.8008 16.3 0.948 Contact Map
4tnwA 3 0.3459 12.8 0.95 Contact Map
3dh4A 3 0.312 11.3 0.951 Contact Map
4oo9A 1 0.3008 9.7 0.953 Contact Map
4pypA 1 0.5752 9.5 0.953 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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