Q9HQX3 - HALSA - GREMLIN Contacts

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Q9HQX3 - Phycocyanin alpha phycocyanobilin lyase-like

UniProt:	Q9HQX3	Sequences: MSDDDESVRAAAIDALTQQEAVDALMRGLDQEVPDSGATWAQAEAFVENLESETPELRMAAANVLGLLGVEDTARPLAKQLQTEEHVGVRARVARALGRIEQPAVTGILVDCLHGEPLKVRREAAESLGRLTTEQALDGLLSVVEDDSEAMRRTAVSSLGRFETAEPVDALVERLGDESDLVRRAAVFSLIEILSNVPPDQSHELRETIVDRMSARSDPSIIKSLIEIIDEGTQLHQRRNATWMLGRVAGDQRTKMDAIEALRELLGEDDDLIAQFAATGLAEIGGASVETSLLEVVETQEYGEDAVAMAAFALGKVGGDRSRQRLERLVDETDSEEVRRRAFSAISKLGGKT	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	353 (274)
Sequences:	5788 (4616)
Seq/√Len:	278.9

ECOLI - YIBA
HALSA - Q9HQX3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
238_R	268_E	5.114	1.00
152_R	177_D	3.592	1.00
52_S	57_L	3.360	1.00
177_D	182_V	3.121	1.00
152_R	156_V	2.554	1.00
49_N	61_A	2.462	1.00
325_R	329_L	2.373	1.00
325_R	328_R	2.347	1.00
76_P	79_K	2.341	1.00
276_F	280_G	2.295	1.00
251_D	254_T	2.264	1.00
309_M	316_K	2.205	1.00
242_T	280_G	2.168	1.00
246_G	249_A	2.158	1.00
307_V	310_A	2.131	1.00
156_V	186_A	2.111	1.00
276_F	279_T	2.095	1.00
324_Q	327_E	2.088	1.00
154_T	157_S	2.087	1.00
156_V	160_G	2.073	1.00
146_D	151_M	2.023	1.00
62_A	66_G	1.967	1.00
239_R	243_W	1.958	1.00
115_G	120_V	1.949	1.00
311_A	315_G	1.928	1.00
143_V	155_A	1.882	1.00
125_A	129_G	1.871	1.00
121_R	146_D	1.853	1.00
90_R	115_G	1.841	1.00
224_S	228_I	1.820	1.00
60_A	64_V	1.818	1.00
171_L	189_S	1.818	1.00
163_E	166_E	1.815	1.00
315_G	318_G	1.814	1.00
269_D	273_I	1.786	1.00
187_V	191_I	1.775	1.00
83_T	89_V	1.750	1.00
240_N	243_W	1.725	1.00
246_G	283_E	1.667	1.00
243_W	276_F	1.663	1.00
278_A	313_A	1.632	1.00
246_G	280_G	1.632	1.00
318_G	321_R	1.629	1.00
304_E	308_A	1.626	1.00
58_R	83_T	1.619	1.00
288_S	291_T	1.597	1.00
312_F	316_K	1.594	1.00
262_L	280_G	1.591	1.00
185_A	189_S	1.567	1.00
313_A	316_K	1.543	1.00
95_R	99_R	1.533	1.00
149_E	153_R	1.530	1.00
91_A	98_G	1.515	1.00
112_C	124_A	1.488	1.00
309_M	312_F	1.479	1.00
91_A	95_R	1.469	1.00
35_D	39_T	1.465	1.00
48_E	52_S	1.448	1.00
79_K	82_Q	1.445	1.00
264_E	267_G	1.442	1.00
316_K	319_G	1.434	1.00
232_G	237_Q	1.434	1.00
89_V	92_R	1.407	1.00
224_S	227_E	1.392	1.00
324_Q	329_L	1.392	1.00
312_F	317_V	1.373	1.00
184_R	187_V	1.372	1.00
296_V	299_T	1.368	1.00
225_L	244_M	1.342	1.00
108_I	112_C	1.333	1.00
326_L	329_L	1.330	1.00
53_E	58_R	1.330	1.00
109_L	127_S	1.319	1.00
54_T	57_L	1.316	1.00
251_D	255_K	1.316	1.00
296_V	306_A	1.315	1.00
69_G	72_D	1.313	1.00
94_A	98_G	1.309	1.00
136_A	140_L	1.300	1.00
126_E	130_R	1.288	1.00
188_F	191_I	1.287	1.00
192_E	195_S	1.284	1.00
154_T	161_R	1.280	1.00
154_T	158_S	1.276	1.00
151_M	154_T	1.269	1.00
324_Q	328_R	1.262	1.00
87_V	90_R	1.261	1.00
36_S	39_T	1.260	1.00
40_W	44_E	1.252	1.00
304_E	307_V	1.251	1.00
49_N	52_S	1.250	1.00
73_T	77_L	1.240	1.00
307_V	311_A	1.225	1.00
169_D	174_R	1.223	1.00
157_S	161_R	1.222	1.00
55_P	60_A	1.222	1.00
35_D	38_A	1.214	1.00
50_L	65_L	1.208	1.00
66_G	96_A	1.205	1.00
51_E	76_P	1.200	1.00
182_V	185_A	1.193	1.00
286_G	289_V	1.185	1.00
265_L	268_E	1.177	1.00
308_A	316_K	1.174	1.00
308_A	315_G	1.174	1.00
73_T	80_Q	1.173	1.00
181_L	185_A	1.167	1.00
250_G	254_T	1.163	1.00
37_G	41_A	1.156	1.00
188_F	192_E	1.151	1.00
228_I	237_Q	1.146	1.00
41_A	45_A	1.136	1.00
251_D	256_M	1.133	1.00
174_R	177_D	1.124	1.00
268_E	273_I	1.122	1.00
277_A	280_G	1.118	1.00
181_L	184_R	1.110	1.00
42_Q	45_A	1.104	1.00
291_T	294_L	1.099	1.00
274_A	282_A	1.092	1.00
94_A	112_C	1.091	1.00
92_R	96_A	1.084	1.00
139_G	143_V	1.083	1.00
275_Q	279_T	1.081	1.00
170_A	174_R	1.077	1.00
136_A	139_G	1.075	1.00
293_L	313_A	1.070	1.00
90_R	95_R	1.066	1.00
268_E	277_A	1.066	1.00
58_R	62_A	1.065	1.00
77_L	81_L	1.061	1.00
163_E	167_P	1.056	1.00
48_E	51_E	1.056	1.00
300_Q	306_A	1.054	1.00
59_M	62_A	1.052	1.00
242_T	277_A	1.044	1.00
77_L	80_Q	1.043	1.00
120_V	123_E	1.040	1.00
142_S	145_E	1.037	1.00
140_L	155_A	1.035	1.00
44_E	49_N	1.035	1.00
75_R	78_A	1.028	1.00
299_T	306_A	1.016	1.00
183_R	187_V	1.013	1.00
136_A	145_E	1.012	1.00
170_A	177_D	1.008	1.00
92_R	95_R	1.004	1.00
296_V	310_A	1.004	1.00
49_N	57_L	1.003	1.00
316_K	320_D	0.995	1.00
95_R	98_G	0.992	1.00
311_A	314_L	0.990	1.00
63_N	68_L	0.990	1.00
235_L	273_I	0.988	1.00
242_T	246_G	0.988	1.00
115_G	124_A	0.984	1.00
80_Q	83_T	0.979	1.00
46_F	64_V	0.979	1.00
135_Q	139_G	0.966	1.00
158_S	161_R	0.964	1.00
37_G	40_W	0.962	1.00
238_R	265_L	0.949	1.00
259_I	262_L	0.947	1.00
315_G	321_R	0.947	1.00
78_A	105_V	0.946	1.00
183_R	186_A	0.930	1.00
279_T	282_A	0.929	1.00
192_E	196_N	0.928	1.00
74_A	77_L	0.925	1.00
312_F	318_G	0.923	1.00
40_W	43_A	0.922	1.00
189_S	193_I	0.919	1.00
111_D	114_H	0.914	1.00
41_A	48_E	0.908	1.00
307_V	318_G	0.906	1.00
190_L	194_L	0.901	1.00
154_T	160_G	0.901	1.00
40_W	48_E	0.897	1.00
223_K	226_I	0.892	1.00
90_R	101_E	0.892	1.00
243_W	246_G	0.886	1.00
170_A	173_E	0.885	1.00
36_S	40_W	0.885	1.00
170_A	175_L	0.884	1.00
317_V	322_S	0.881	1.00
148_S	151_M	0.879	1.00
152_R	157_S	0.878	1.00
73_T	78_A	0.877	1.00
238_R	242_T	0.875	1.00
59_M	63_N	0.873	1.00
242_T	250_G	0.872	1.00
153_R	157_S	0.870	1.00
285_G	297_V	0.869	1.00
64_V	67_L	0.869	1.00
139_G	142_S	0.868	1.00
77_L	96_A	0.868	1.00
122_R	131_L	0.867	1.00
153_R	156_V	0.863	1.00
145_E	148_S	0.859	1.00
285_G	289_V	0.854	1.00
146_D	155_A	0.852	1.00
113_L	125_A	0.852	1.00
124_A	128_L	0.852	1.00
308_A	312_F	0.851	1.00
253_R	256_M	0.848	1.00
49_N	64_V	0.845	1.00
35_D	44_E	0.842	1.00
134_E	142_S	0.841	1.00
224_S	229_I	0.840	1.00
121_R	125_A	0.839	1.00
87_V	95_R	0.838	1.00
121_R	124_A	0.837	1.00
123_E	126_E	0.835	1.00
63_N	66_G	0.832	1.00
56_E	60_A	0.832	1.00
273_I	277_A	0.829	1.00
35_D	42_Q	0.828	1.00
164_T	167_P	0.827	1.00
239_R	270_D	0.826	1.00
306_A	309_M	0.826	1.00
36_S	42_Q	0.826	1.00
274_A	277_A	0.824	1.00
149_E	179_S	0.819	0.99
270_D	273_I	0.818	0.99
243_W	247_R	0.813	0.99
60_A	67_L	0.813	0.99
129_G	132_T	0.812	0.99
262_L	293_L	0.811	0.99
129_G	157_S	0.811	0.99
57_L	61_A	0.808	0.99
240_N	248_V	0.799	0.99
239_R	242_T	0.797	0.99
306_A	311_A	0.796	0.99
38_A	45_A	0.796	0.99
257_D	260_E	0.792	0.99
112_C	115_G	0.787	0.99
118_L	122_R	0.785	0.99
278_A	282_A	0.780	0.99
233_T	237_Q	0.779	0.99
191_I	194_L	0.778	0.99
246_G	279_T	0.776	0.99
186_A	190_L	0.774	0.99
261_A	264_E	0.772	0.99
274_A	299_T	0.769	0.99
222_I	245_L	0.768	0.99
138_D	142_S	0.765	0.99
99_R	102_Q	0.764	0.99
111_D	116_E	0.762	0.99
97_L	101_E	0.760	0.99
254_T	257_D	0.759	0.99
102_Q	130_R	0.758	0.99
42_Q	64_V	0.758	0.99
153_R	179_S	0.757	0.99
61_A	64_V	0.752	0.99
120_V	126_E	0.750	0.99
160_G	188_F	0.748	0.99
287_A	298_E	0.744	0.99
275_Q	282_A	0.744	0.99
309_M	318_G	0.742	0.99
91_A	97_L	0.741	0.99
96_A	101_E	0.740	0.99
316_K	322_S	0.739	0.99
38_A	42_Q	0.738	0.99
292_S	295_E	0.737	0.99
147_D	151_M	0.736	0.99
140_L	151_M	0.735	0.99
53_E	57_L	0.731	0.99
86_H	90_R	0.730	0.99
235_L	240_N	0.719	0.99
83_T	105_V	0.719	0.99
315_G	320_D	0.713	0.99
274_A	280_G	0.712	0.99
49_N	62_A	0.711	0.99
270_D	280_G	0.709	0.99
98_G	101_E	0.703	0.98
174_R	186_A	0.699	0.98
240_N	283_E	0.698	0.98
46_F	65_L	0.698	0.98
88_G	94_A	0.697	0.98
269_D	274_A	0.695	0.98
108_I	111_D	0.695	0.98
52_S	65_L	0.694	0.98
252_Q	280_G	0.694	0.98
300_Q	311_A	0.692	0.98
286_G	293_L	0.691	0.98
51_E	60_A	0.691	0.98
232_G	238_R	0.690	0.98
285_G	296_V	0.689	0.98
98_G	127_S	0.687	0.98
229_I	241_A	0.686	0.98
271_D	278_A	0.686	0.98
294_L	320_D	0.685	0.98
266_L	278_A	0.681	0.98
299_T	310_A	0.678	0.98
36_S	41_A	0.676	0.98
64_V	68_L	0.676	0.98
230_D	261_A	0.674	0.98
285_G	291_T	0.671	0.98
38_A	41_A	0.670	0.98
74_A	78_A	0.669	0.98
247_R	283_E	0.667	0.98
312_F	315_G	0.665	0.98
244_M	247_R	0.662	0.98
188_F	196_N	0.655	0.98
257_D	261_A	0.651	0.97
163_E	172_V	0.651	0.97
225_L	228_I	0.650	0.97
76_P	80_Q	0.649	0.97
308_A	314_L	0.649	0.97
62_A	96_A	0.648	0.97
125_A	155_A	0.647	0.97
135_Q	138_D	0.647	0.97
260_E	263_R	0.646	0.97
271_D	275_Q	0.645	0.97
242_T	249_A	0.644	0.97
222_I	226_I	0.643	0.97
151_M	155_A	0.642	0.97
311_A	318_G	0.642	0.97
118_L	121_R	0.639	0.97
240_N	249_A	0.637	0.97
58_R	68_L	0.637	0.97
109_L	124_A	0.634	0.97
129_G	158_S	0.633	0.97
239_R	246_G	0.630	0.97
89_V	101_E	0.629	0.97
50_L	62_A	0.627	0.97
51_E	79_K	0.626	0.97
94_A	127_S	0.622	0.97
134_E	138_D	0.621	0.97
235_L	270_D	0.620	0.97
305_D	312_F	0.617	0.96
41_A	44_E	0.616	0.96
55_P	64_V	0.616	0.96
275_Q	305_D	0.615	0.96
193_I	199_P	0.614	0.96
267_G	295_E	0.612	0.96
288_S	292_S	0.612	0.96
258_A	268_E	0.612	0.96
234_Q	237_Q	0.611	0.96
39_T	44_E	0.610	0.96
39_T	48_E	0.610	0.96
55_P	59_M	0.609	0.96
222_I	228_I	0.605	0.96
236_H	239_R	0.604	0.96
266_L	284_I	0.601	0.96
177_D	183_R	0.598	0.96
280_G	283_E	0.597	0.96
189_S	192_E	0.593	0.96
239_R	272_L	0.593	0.96
259_I	277_A	0.593	0.96
309_M	314_L	0.593	0.96
246_G	258_A	0.591	0.95
45_A	54_T	0.590	0.95
230_D	264_E	0.588	0.95
62_A	65_L	0.587	0.95
224_S	237_Q	0.587	0.95
60_A	66_G	0.585	0.95
187_V	197_V	0.585	0.95
287_A	300_Q	0.583	0.95
52_S	58_R	0.583	0.95
45_A	49_N	0.581	0.95
43_A	49_N	0.580	0.95
310_A	313_A	0.580	0.95
228_I	232_G	0.577	0.95
59_M	64_V	0.577	0.95
319_G	322_S	0.576	0.95
308_A	311_A	0.575	0.95
51_E	82_Q	0.574	0.95
45_A	48_E	0.569	0.94
247_R	282_A	0.566	0.94
219_P	223_K	0.565	0.94
270_D	278_A	0.564	0.94
265_L	273_I	0.564	0.94
157_S	160_G	0.557	0.94
94_A	123_E	0.554	0.94
139_G	144_V	0.554	0.94
140_L	144_V	0.548	0.93
141_L	145_E	0.544	0.93
157_S	185_A	0.541	0.93
148_S	158_S	0.540	0.93
170_A	190_L	0.539	0.93
289_V	293_L	0.537	0.93
236_H	249_A	0.536	0.93
224_S	263_R	0.536	0.93
309_M	315_G	0.534	0.92
51_E	57_L	0.532	0.92
42_Q	51_E	0.531	0.92
297_V	310_A	0.530	0.92
152_R	160_G	0.529	0.92
88_G	118_L	0.528	0.92
65_L	73_T	0.528	0.92
38_A	43_A	0.523	0.92
152_R	183_R	0.523	0.92
275_Q	309_M	0.521	0.91
85_E	90_R	0.520	0.91
273_I	283_E	0.518	0.91
265_L	270_D	0.517	0.91
188_F	195_S	0.517	0.91
117_P	122_R	0.517	0.91
37_G	52_S	0.513	0.91
187_V	244_M	0.512	0.91
94_A	99_R	0.510	0.91
240_N	246_G	0.510	0.91
51_E	55_P	0.507	0.90
45_A	51_E	0.506	0.90
57_L	62_A	0.503	0.90
274_A	306_A	0.503	0.90
134_E	143_V	0.502	0.90
309_M	322_S	0.502	0.90
240_N	244_M	0.500	0.90
293_L	310_A	0.500	0.90

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1wa5B	1	0.9178	100	0.437	Contact Map
1b3uA	1	0.915	100	0.443	Contact Map
3tpoA	1	0.881	99.9	0.45	Contact Map
4b8jA	1	0.8612	99.9	0.484	Contact Map
2vglB	1	0.9547	99.9	0.491	Contact Map
3nowA	3	0.9603	99.9	0.497	Contact Map
4d4zA	1	0.8017	99.9	0.497	Contact Map
4uafB	1	0.8499	99.9	0.5	Contact Map
4rv1A	1	0.8414	99.9	0.5	Contact Map
1u6gC	1	0.8272	99.9	0.502	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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