GREMLIN Database
TPIS - Triosephosphate isomerase
UniProt: Q9HQS4 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 214 (209)
Sequences: 301 (222)
Seq/√Len: 15.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
67_S88_H4.4021.00
4_L110_V4.3901.00
81_T109_T3.9131.00
73_V78_A3.6561.00
163_D166_V3.6531.00
102_A109_T3.4720.99
37_Q40_D3.3820.99
126_G163_D3.1420.99
202_D205_A3.0800.98
113_A119_V3.0420.98
42_G46_D2.8520.97
207_L211_V2.6040.95
140_T146_Q2.5890.95
160_E168_V2.5490.95
63_S170_C2.4210.93
37_Q72_S2.3990.93
131_A192_L2.3830.92
81_T102_A2.3800.92
83_L95_I2.2660.90
140_T175_T2.2440.90
206_A209_D2.2270.89
142_T147_A2.1920.89
56_V81_T2.1680.88
71_E101_A2.1290.87
91_R94_D2.1090.87
41_I53_A2.0360.85
36_P41_I2.0210.84
141_G175_T1.9900.83
101_A105_A1.9780.83
5_V207_L1.9720.83
140_T143_P1.9620.82
119_V155_A1.9140.81
141_G146_Q1.8070.77
130_V166_V1.7780.75
45_A78_A1.7670.75
70_A101_A1.7380.74
15_V44_V1.6910.72
143_P147_A1.6610.70
38_P68_V1.6170.68
141_G174_I1.6030.67
146_Q178_E1.5710.66
132_V170_C1.5670.66
28_T208_A1.5450.64
204_R208_A1.5210.63
58_P94_D1.5120.63
152_V182_S1.4770.61
15_V19_E1.4070.57
131_A169_Y1.4040.57
119_V159_A1.3900.56
143_P146_Q1.3850.56
41_I78_A1.3310.53
141_G145_S1.2930.50
140_T174_I1.2850.50
75_D106_G1.2820.50
37_Q69_L1.2680.49
93_A115_N1.2600.49
89_R93_A1.2590.49
5_V9_A1.2550.48
89_R113_A1.2410.48
134_P190_G1.2360.47
41_I76_N1.2360.47
7_L14_P1.2340.47
31_T50_T1.2200.46
149_P186_L1.2130.46
103_E128_D1.2000.45
82_L131_A1.1880.45
34_V68_V1.1870.44
126_G165_S1.1780.44
59_T69_L1.1680.43
170_C188_A1.1640.43
193_L207_L1.1590.43
182_S185_D1.1560.43
186_L190_G1.1340.42
3_V207_L1.1300.41
56_V69_L1.1210.41
19_E101_A1.1110.40
15_V47_T1.0840.39
83_L125_L1.0780.38
109_T140_T1.0720.38
33_A52_Y1.0410.37
55_H134_P1.0310.36
175_T196_G1.0270.36
15_V43_R1.0140.35
143_P174_I1.0100.35
177_G209_D1.0080.35
132_V151_I1.0010.34
123_A162_V0.9990.34
96_D162_V0.9830.34
96_D181_V0.9740.33
86_S131_A0.9510.32
63_S196_G0.9450.32
18_A47_T0.9440.32
92_L122_A0.9430.32
73_V109_T0.9430.32
88_H211_V0.9420.31
23_D204_R0.9400.31
73_V107_L0.9380.31
31_T185_D0.9340.31
5_V21_A0.9310.31
93_A114_N0.9290.31
60_E104_R0.9180.30
3_V191_V0.9110.30
193_L211_V0.9110.30
181_V185_D0.9080.30
33_A80_G0.9080.30
21_A32_I0.9080.30
9_A72_S0.9060.30
96_D126_G0.8930.29
112_C138_I0.8880.29
135_P149_P0.8830.29
64_H69_L0.8830.29
9_A181_V0.8820.29
86_S194_A0.8800.29
9_A37_Q0.8790.28
135_P171_G0.8780.28
103_E164_P0.8770.28
145_S174_I0.8730.28
3_V147_A0.8720.28
60_E69_L0.8690.28
24_V89_R0.8670.28
49_A126_G0.8660.28
125_L211_V0.8630.28
51_T57_S0.8620.28
89_R95_I0.8620.28
68_V119_V0.8610.28
56_V73_V0.8590.28
75_D158_A0.8590.28
94_D165_S0.8570.27
100_A117_A0.8520.27
152_V183_A0.8380.27
15_V18_A0.8340.26
3_V209_D0.8340.26
61_H69_L0.8300.26
135_P194_A0.8280.26
35_A41_I0.8260.26
143_P182_S0.8260.26
71_E157_A0.8260.26
98_S159_A0.8200.26
157_A161_A0.8110.25
146_Q174_I0.8060.25
80_G135_P0.7980.25
96_D118_Q0.7940.25
12_C75_D0.7920.25
123_A169_Y0.7890.24
65_T86_S0.7880.24
131_A193_L0.7790.24
61_H68_V0.7770.24
65_T149_P0.7760.24
24_V32_I0.7760.24
132_V183_A0.7740.24
19_E126_G0.7730.24
37_Q184_G0.7710.24
123_A166_V0.7680.24
12_C109_T0.7630.23
201_D204_R0.7630.23
106_G202_D0.7620.23
70_A105_A0.7610.23
114_N121_A0.7580.23
30_A56_V0.7540.23
86_S89_R0.7530.23
155_A188_A0.7490.23
108_D128_D0.7470.23
40_D119_V0.7440.23
86_S135_P0.7430.23
99_V111_V0.7420.23
43_R209_D0.7350.22
60_E100_A0.7350.22
45_A102_A0.7350.22
198_A202_D0.7340.22
144_V151_I0.7340.22
203_P207_L0.7330.22
206_A211_V0.7280.22
99_V201_D0.7260.22
118_Q122_A0.7260.22
27_T208_A0.7230.22
55_H90_R0.7220.22
92_L121_A0.7190.22
55_H88_H0.7190.22
47_T90_R0.7130.21
60_E99_V0.7110.21
111_V122_A0.7090.21
112_C192_L0.7070.21
77_G164_P0.7050.21
152_V206_A0.7040.21
5_V83_L0.6970.21
5_V193_L0.6950.21
137_L172_A0.6920.21
23_D26_E0.6900.21
65_T144_V0.6900.21
34_V98_S0.6890.21
12_C99_V0.6890.21
121_A168_V0.6870.20
12_C159_A0.6870.20
188_A200_A0.6850.20
140_T176_T0.6830.20
65_T134_P0.6780.20
124_A188_A0.6720.20
24_V28_T0.6720.20
146_Q175_T0.6690.20
140_T151_I0.6660.20
57_S88_H0.6660.20
140_T145_S0.6620.20
154_D157_A0.6610.20
147_A191_V0.6610.20
16_A19_E0.6600.19
147_A203_P0.6600.19
126_G157_A0.6590.19
17_I20_A0.6590.19
98_S208_A0.6580.19
134_P186_L0.6560.19
97_G100_A0.6550.19
47_T179_D0.6530.19
159_A174_I0.6510.19
47_T50_T0.6490.19
24_V104_R0.6490.19
16_A44_V0.6480.19
99_V109_T0.6480.19
7_L47_T0.6470.19
128_D175_T0.6460.19
31_T37_Q0.6450.19
68_V83_L0.6450.19
7_L18_A0.6440.19
34_V81_T0.6410.19
126_G181_V0.6380.19
4_L169_Y0.6370.19
13_D207_L0.6330.19
13_D16_A0.6320.19
41_I198_A0.6320.19
65_T135_P0.6270.18
145_S178_E0.6260.18
146_Q196_G0.6260.18
155_A168_V0.6250.18
147_A156_V0.6210.18
28_T32_I0.6180.18
44_V51_T0.6160.18
116_P154_D0.6160.18
113_A118_Q0.6160.18
9_A206_A0.6130.18
185_D207_L0.6110.18
17_I207_L0.6080.18
22_A48_G0.6080.18
5_V209_D0.6070.18
121_A124_A0.6060.18
7_L102_A0.6050.18
101_A104_R0.6030.18
44_V74_A0.5940.17
15_V179_D0.5940.17
75_D97_G0.5930.17
38_P202_D0.5910.17
14_P50_T0.5900.17
80_G149_P0.5880.17
137_L190_G0.5860.17
29_P186_L0.5840.17
93_A97_G0.5840.17
41_I91_R0.5830.17
20_A209_D0.5830.17
57_S169_Y0.5830.17
53_A76_N0.5810.17
16_A160_E0.5800.17
156_V193_L0.5790.17
116_P158_A0.5780.17
196_G206_A0.5780.17
122_A166_V0.5770.17
6_N190_G0.5770.17
159_A181_V0.5760.17
30_A165_S0.5680.16
152_V204_R0.5680.16
36_P152_V0.5610.16
38_P67_S0.5590.16
36_P65_T0.5590.16
97_G101_A0.5590.16
143_P206_A0.5560.16
134_P144_V0.5540.16
74_A153_S0.5540.16
5_V198_A0.5500.16
64_H191_V0.5500.16
80_G89_R0.5500.16
65_T192_L0.5490.16
149_P171_G0.5460.16
80_G171_G0.5440.16
40_D69_L0.5440.16
56_V102_A0.5430.16
106_G176_T0.5430.16
65_T82_L0.5420.16
52_Y80_G0.5420.16
116_P129_A0.5400.16
158_A161_A0.5400.16
13_D101_A0.5370.15
64_H115_N0.5360.15
62_G84_N0.5360.15
134_P137_L0.5320.15
160_E185_D0.5310.15
134_P139_G0.5290.15
171_G193_L0.5270.15
149_P194_A0.5270.15
14_P17_I0.5270.15
56_V82_L0.5240.15
99_V122_A0.5170.15
76_N88_H0.5160.15
60_E64_H0.5160.15
82_L112_C0.5150.15
36_P53_A0.5130.15
7_L17_I0.5130.15
6_N186_L0.5120.15
152_V168_V0.5120.15
170_C183_A0.5080.15
23_D205_A0.5080.15
40_D78_A0.5070.15
55_H172_A0.5060.15
73_V114_N0.5050.15
156_V200_A0.5040.15
93_A118_Q0.5030.14
38_P189_S0.5020.14
35_A135_P0.5000.14
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4y9aA 2 0.972 100 0.505 Contact Map
4y96A 2 0.972 100 0.508 Contact Map
4y90A 3 0.972 100 0.515 Contact Map
4y8fA 2 0.9626 100 0.515 Contact Map
2h6rA 3 0.9579 100 0.522 Contact Map
4g1kA 2 0.972 100 0.545 Contact Map
2jgqA 2 0.9533 100 0.56 Contact Map
4mknA 2 0.986 100 0.562 Contact Map
4mvaA 2 0.9813 100 0.563 Contact Map
4nvtA 2 0.972 100 0.563 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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