GREMLIN Database
GCS2 - Glutamate--cysteine ligase
UniProt: Q9HPZ9 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 360 (334)
Sequences: 3169 (2083)
Seq/√Len: 114.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
176_D343_R6.1711.00
65_P76_A4.5421.00
70_V256_E3.7081.00
17_V73_A3.5551.00
35_D39_Y3.2881.00
168_N338_G3.2691.00
74_P252_D3.0491.00
26_E94_R3.0251.00
337_S340_Q2.9631.00
331_D335_D2.9541.00
239_V258_A2.9321.00
14_T141_G2.8761.00
69_G72_E2.8601.00
68_N72_E2.8541.00
67_L72_E2.8291.00
166_S254_F2.7281.00
113_E118_R2.6651.00
345_I349_H2.6541.00
180_A340_Q2.5701.00
318_D330_R2.5441.00
180_A343_R2.5201.00
145_P206_Q2.4561.00
210_R214_E2.4081.00
171_Y346_L2.3791.00
148_A230_P2.3271.00
299_D340_Q2.2651.00
201_S204_A2.2551.00
200_E204_A2.2201.00
97_G244_A2.1991.00
264_E323_R2.1301.00
312_D315_T2.0841.00
292_R296_D2.0681.00
74_P78_R2.0581.00
330_R334_D2.0261.00
25_D28_G2.0001.00
167_A337_S1.9891.00
245_Q254_F1.9781.00
212_M228_V1.9161.00
155_M239_V1.9131.00
257_Y324_L1.9081.00
340_Q344_R1.9001.00
78_R82_A1.8761.00
268_R273_P1.8401.00
247_D250_V1.7801.00
152_S237_V1.7771.00
51_L84_L1.7581.00
338_G342_Q1.7441.00
13_G263_T1.7181.00
311_V316_V1.6871.00
23_V93_L1.6791.00
27_H92_G1.6601.00
64_T67_L1.6491.00
150_W269_Y1.6421.00
180_A339_A1.6411.00
158_H261_L1.6411.00
299_D314_G1.6291.00
22_F134_A1.6261.00
67_L76_A1.6241.00
24_V96_A1.6101.00
318_D322_D1.5811.00
16_G20_E1.5401.00
35_D41_G1.5371.00
74_P249_A1.5291.00
77_I248_P1.5231.00
185_K188_E1.5201.00
52_D63_Q1.5171.00
263_T267_Q1.4941.00
85_V94_R1.4901.00
34_S37_L1.4881.00
35_D38_V1.4811.00
144_D147_K1.4791.00
246_A338_G1.4741.00
125_Q129_H1.4661.00
332_V336_V1.4621.00
71_A75_A1.4611.00
150_W272_H1.4551.00
344_R348_T1.4521.00
101_H104_A1.4521.00
101_H109_H1.4391.00
327_S330_R1.4201.00
301_S310_A1.4151.00
30_P37_L1.4141.00
17_V255_V1.4101.00
311_V319_R1.4031.00
202_Y206_Q1.4001.00
322_D325_G1.3981.00
26_E89_S1.3781.00
292_R300_A1.3671.00
253_A329_I1.3651.00
116_R220_D1.3621.00
37_L42_E1.3621.00
305_R309_G1.3611.00
282_R285_L1.3581.00
105_R108_E1.3581.00
161_V317_V1.3541.00
152_S230_P1.3531.00
100_L172_W1.3511.00
316_V320_E1.3381.00
139_H236_T1.3321.00
328_G331_D1.3291.00
99_G244_A1.3221.00
136_L251_V1.3201.00
317_V321_C1.3161.00
60_V84_L1.3151.00
30_P104_A1.3121.00
288_A292_R1.3111.00
321_C326_V1.3021.00
75_A79_E1.3011.00
253_A328_G1.2920.99
191_P194_G1.2830.99
83_A86_A1.2810.99
37_L41_G1.2670.99
171_Y343_R1.2650.99
315_T318_D1.2580.99
78_R248_P1.2580.99
30_P33_G1.2570.99
272_H278_V1.2560.99
204_A207_A1.2540.99
143_D147_K1.2480.99
250_V341_Q1.2470.99
115_P118_R1.2430.99
29_V33_G1.2170.99
179_L185_K1.2120.99
172_W177_T1.2060.99
146_D202_Y1.2020.99
314_G333_Y1.1950.99
66_T141_G1.1950.99
320_E324_L1.1880.99
22_F59_V1.1860.99
249_A328_G1.1750.99
225_W229_R1.1720.99
292_R310_A1.1600.99
155_M262_V1.1540.99
72_E76_A1.1490.99
138_I255_V1.1490.99
346_L350_G1.1440.99
130_R189_G1.1240.99
318_D333_Y1.1140.98
179_L294_M1.1110.98
153_N200_E1.1070.98
319_R323_R1.0890.98
264_E267_Q1.0880.98
21_F62_T1.0830.98
301_S312_D1.0770.98
222_G225_W1.0740.98
348_T351_E1.0710.98
162_L258_A1.0700.98
179_L295_R1.0690.98
26_E92_G1.0690.98
322_D347_D1.0680.98
82_A86_A1.0660.98
70_V252_D1.0580.98
39_Y42_E1.0580.98
111_H121_L1.0510.98
40_E91_H1.0490.98
14_T66_T1.0450.98
163_L166_S1.0450.98
302_F313_L1.0420.98
180_A297_G1.0420.98
151_V262_V1.0300.97
57_K113_E1.0250.97
346_L349_H1.0170.97
75_A78_R1.0040.97
33_G39_Y0.9970.97
77_I252_D0.9910.97
285_L303_V0.9900.97
205_F209_E0.9880.97
46_P83_A0.9850.97
61_E240_R0.9750.96
163_L184_A0.9720.96
81_R84_L0.9640.96
154_R268_R0.9550.96
203_A207_A0.9550.96
124_I187_F0.9520.96
208_F211_R0.9470.96
321_C329_I0.9450.96
31_T38_V0.9410.96
345_I348_T0.9400.96
23_V95_I0.9350.95
207_A211_R0.9300.95
171_Y176_D0.9290.95
30_P39_Y0.9290.95
180_A299_D0.9280.95
154_R265_Y0.9270.95
155_M258_A0.9250.95
172_W186_I0.9250.95
22_F99_G0.9240.95
163_L241_A0.9200.95
287_D291_W0.9160.95
15_L140_V0.9160.95
133_T187_F0.9140.95
166_S242_P0.9100.95
155_M237_V0.9090.95
147_K266_A0.9070.95
97_G248_P0.9060.95
176_D180_A0.9060.95
30_P34_S0.9030.94
211_R215_H0.8990.94
112_A117_Y0.8970.94
269_Y272_H0.8900.94
147_K150_W0.8840.94
150_W154_R0.8800.94
98_A168_N0.8800.94
176_D347_D0.8800.94
161_V307_T0.8730.93
41_G90_E0.8690.93
87_Y91_H0.8680.93
343_R347_D0.8640.93
151_V237_V0.8600.93
142_V237_V0.8550.93
140_V259_H0.8520.93
162_L242_P0.8480.92
347_D350_G0.8480.92
188_E287_D0.8470.92
344_R347_D0.8460.92
50_R136_L0.8440.92
20_E61_E0.8440.92
321_C333_Y0.8420.92
198_A201_S0.8420.92
317_V320_E0.8410.92
314_G318_D0.8390.92
86_A90_E0.8350.92
342_Q345_I0.8310.92
162_L239_V0.8290.92
74_P248_P0.8280.92
127_P130_R0.8220.91
165_L332_V0.8190.91
264_E268_R0.8180.91
200_E207_A0.8160.91
16_G61_E0.8140.91
36_E39_Y0.8120.91
167_A180_A0.8120.91
98_A342_Q0.8080.91
24_V94_R0.8060.90
50_R65_P0.8040.90
199_F205_F0.8030.90
181_S243_D0.8010.90
118_R122_D0.8010.90
136_L244_A0.7990.90
178_G181_S0.7950.90
291_W295_R0.7940.90
164_A293_A0.7920.90
32_A37_L0.7870.89
260_A324_L0.7860.89
33_G37_L0.7860.89
58_F132_T0.7810.89
335_D341_Q0.7790.89
203_A206_Q0.7780.89
296_D300_A0.7750.89
151_V266_A0.7740.89
41_G91_H0.7700.88
227_D290_K0.7690.88
111_H118_R0.7680.88
317_V333_Y0.7660.88
242_P254_F0.7650.88
160_P243_D0.7640.88
19_E81_R0.7630.88
331_D334_D0.7610.88
163_L167_A0.7600.88
56_F133_T0.7590.88
119_S122_D0.7540.87
55_L120_Q0.7530.87
37_L91_H0.7520.87
153_N198_A0.7510.87
321_C324_L0.7470.87
316_V321_C0.7470.87
164_A298_H0.7460.87
138_I239_V0.7420.86
77_I136_L0.7360.86
36_E40_E0.7350.86
38_V42_E0.7320.86
15_L73_A0.7310.86
298_H333_Y0.7300.85
56_F132_T0.7280.85
61_E137_H0.7280.85
131_N187_F0.7250.85
62_T84_L0.7210.85
306_E309_G0.7170.84
143_D266_A0.7160.84
223_E227_D0.7130.84
283_R286_L0.7120.84
326_V332_V0.7120.84
130_R225_W0.7100.84
245_Q338_G0.7100.84
33_G93_L0.7080.84
316_V322_D0.7060.84
67_L74_P0.7060.84
152_S228_V0.7050.83
221_R232_S0.7040.83
20_E240_R0.7040.83
90_E344_R0.7020.83
127_P185_K0.7020.83
50_R62_T0.7010.83
138_I242_P0.7000.83
250_V338_G0.7000.83
126_Y129_H0.6990.83
319_R330_R0.6990.83
29_V32_A0.6940.82
148_A205_F0.6920.82
75_A86_A0.6910.82
222_G232_S0.6900.82
159_M239_V0.6900.82
182_A186_I0.6870.82
55_L112_A0.6850.82
128_Q131_N0.6840.82
184_A294_M0.6840.82
15_L70_V0.6820.81
15_L259_H0.6810.81
127_P188_E0.6800.81
99_G261_L0.6790.81
30_P101_H0.6790.81
138_I259_H0.6770.81
228_V237_V0.6760.81
149_V153_N0.6740.81
341_Q345_I0.6720.80
59_V124_I0.6720.80
248_P252_D0.6710.80
70_V261_L0.6700.80
295_R299_D0.6690.80
304_A319_R0.6670.80
72_E334_D0.6660.80
29_V108_E0.6640.80
130_R186_I0.6640.80
265_Y269_Y0.6620.79
249_A252_D0.6610.79
220_D223_E0.6610.79
153_N204_A0.6590.79
70_V259_H0.6570.79
257_Y320_E0.6570.79
152_S156_R0.6510.78
280_G329_I0.6490.78
54_E61_E0.6490.78
57_K112_A0.6480.78
230_P246_A0.6480.78
308_Q333_Y0.6470.78
33_G36_E0.6470.78
164_A167_A0.6470.78
332_V338_G0.6460.78
57_K110_E0.6460.78
168_N260_A0.6460.78
185_K294_M0.6430.77
57_K109_H0.6420.77
137_H236_T0.6410.77
195_L198_A0.6400.77
77_I81_R0.6390.77
125_Q296_D0.6380.77
163_L290_K0.6340.76
187_F227_D0.6320.76
257_Y317_V0.6320.76
139_H229_R0.6320.76
188_E291_W0.6300.76
46_P49_G0.6290.76
329_I333_Y0.6270.76
97_G245_Q0.6220.75
314_G334_D0.6190.75
156_R286_L0.6180.75
79_E82_A0.6160.74
170_P181_S0.6160.74
67_L73_A0.6160.74
34_S38_V0.6150.74
311_V315_T0.6140.74
61_E238_E0.6130.74
179_L225_W0.6120.74
23_V88_A0.6120.74
220_D231_H0.6110.74
52_D114_K0.6100.74
30_P103_G0.6090.74
300_A305_R0.6090.74
177_T182_A0.6080.73
68_N175_F0.6060.73
289_N302_F0.6050.73
131_N190_L0.6050.73
62_T80_V0.6010.73
34_S93_L0.5990.72
213_V220_D0.5980.72
346_L351_E0.5980.72
33_G57_K0.5960.72
17_V135_G0.5940.72
21_F95_I0.5940.72
293_A302_F0.5940.72
21_F84_L0.5930.72
286_L289_N0.5930.72
136_L245_Q0.5920.71
77_I251_V0.5910.71
293_A300_A0.5910.71
116_R120_Q0.5900.71
212_M288_A0.5880.71
126_Y192_N0.5880.71
119_S123_R0.5870.71
137_H246_A0.5850.71
145_P232_S0.5850.71
248_P317_V0.5840.70
14_T68_N0.5820.70
195_L287_D0.5810.70
330_R333_Y0.5800.70
180_A337_S0.5780.70
104_A132_T0.5780.70
75_A82_A0.5760.69
22_F96_A0.5750.69
146_D347_D0.5740.69
179_L189_G0.5720.69
229_R236_T0.5700.69
256_E326_V0.5700.69
64_T139_H0.5680.68
35_D40_E0.5670.68
181_S184_A0.5670.68
273_P278_V0.5670.68
15_L64_T0.5660.68
327_S331_D0.5660.68
55_L130_R0.5650.68
56_F124_I0.5640.68
25_D29_V0.5630.68
220_D224_L0.5630.68
187_F193_T0.5620.68
12_S86_A0.5610.67
49_G119_S0.5610.67
213_V216_G0.5600.67
116_R261_L0.5600.67
81_R85_V0.5590.67
50_R80_V0.5590.67
156_R195_L0.5590.67
141_G187_F0.5580.67
17_V64_T0.5580.67
66_T248_P0.5540.66
79_E327_S0.5540.66
130_R172_W0.5530.66
180_A341_Q0.5520.66
117_Y121_L0.5520.66
161_V313_L0.5520.66
165_L311_V0.5520.66
28_G97_G0.5520.66
24_V237_V0.5520.66
262_V266_A0.5510.66
132_T187_F0.5510.66
225_W231_H0.5510.66
38_V41_G0.5510.66
49_G115_P0.5480.66
150_W268_R0.5470.65
100_L182_A0.5470.65
117_Y120_Q0.5450.65
226_Y229_R0.5440.65
316_V319_R0.5420.65
100_L186_I0.5420.65
301_S305_R0.5410.65
57_K63_Q0.5400.64
143_D270_D0.5390.64
163_L293_A0.5370.64
125_Q178_G0.5370.64
207_A210_R0.5360.64
108_E122_D0.5360.64
13_G140_V0.5350.64
130_R190_L0.5340.64
25_D38_V0.5330.63
260_A321_C0.5310.63
324_L348_T0.5300.63
197_T200_E0.5300.63
326_V329_I0.5290.63
147_K270_D0.5270.63
55_L208_F0.5260.62
184_A187_F0.5240.62
59_V133_T0.5230.62
252_D255_V0.5230.62
102_P177_T0.5210.62
315_T319_R0.5210.62
311_V314_G0.5200.62
115_P119_S0.5190.61
162_L257_Y0.5180.61
39_Y51_L0.5180.61
95_I346_L0.5180.61
32_A93_L0.5180.61
303_V310_A0.5170.61
180_A336_V0.5170.61
64_T76_A0.5170.61
32_A39_Y0.5170.61
58_F131_N0.5160.61
143_D263_T0.5150.61
60_V82_A0.5150.61
347_D351_E0.5140.61
284_E288_A0.5140.61
322_D331_D0.5130.61
101_H302_F0.5130.61
179_L182_A0.5130.61
228_V234_H0.5120.60
192_N212_M0.5120.60
31_T42_E0.5110.60
106_W109_H0.5100.60
338_G341_Q0.5080.60
59_V134_A0.5080.60
149_V212_M0.5060.60
58_F80_V0.5060.60
64_T155_M0.5050.59
197_T283_R0.5050.59
73_A252_D0.5050.59
165_L204_A0.5050.59
135_G139_H0.5040.59
137_H244_A0.5040.59
322_D330_R0.5040.59
56_F111_H0.5030.59
310_A313_L0.5030.59
245_Q251_V0.5010.59
55_L187_F0.5000.59
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1r8gA 2 0.95 100 0.237 Contact Map
2gwdA 2 0.9722 100 0.263 Contact Map
3nztA 1 0.9472 100 0.4 Contact Map
1va6A 1 0.9417 100 0.416 Contact Map
3ln6A 2 0.9222 100 0.425 Contact Map
3ln7A 2 0.9306 100 0.429 Contact Map
3ig5A 1 0.9806 100 0.507 Contact Map
4hppA 3 0.7722 99 0.867 Contact Map
3fkyA 8 0.5611 98.3 0.895 Contact Map
4baxA 5 0.5694 98.3 0.895 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0105 seconds.