GCS2 - HALSA - GREMLIN Contacts

Home
GREMLIN
- Submit - Monomer
- Submit - Complex
Contacts
Metagenomics
- Contacts
- Structures
Structures
- List
Old Projects
- Complexes (2014)
- PFAM (2013)

GCS2 - Glutamate--cysteine ligase

UniProt:	Q9HPZ9	Sequences: MDVGSPEAFSESGTLGVEEEFFVVDEHGVPTAGSDELVYEGEPPEPIAGRLDHELFKFVVETQTPTLNGVAEAPAAIREVRAALVAYASEHGLRIAGAGLHPGARWREHEHAEKPRYRSQLDRIQYPQHRNTTAGLHIHVGVDDPDKAVWVSNRMRWHMPVLLALSANSPYWNGFDTGLASARAKIFEGLPNTGLPTAFESYAAFQAFERRMVEHGGIEDRGELWYDVRPHSGHGTVEVRAPDAQADPAVVDAFVEYAHALVTEYAQRYDDHPDPFSVTGLRRELLDANKWRAMRDGHDASFVARETQGAVDLGTVVDRECDRLGVSGIRDVYDDVSGAQQQRRILDTHGEKRLYNHLSL	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	360 (334)
Sequences:	3169 (2083)
Seq/√Len:	114.0

ECOLI - GCS2
HALSA - GCS2

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
176_D	343_R	6.171	1.00
65_P	76_A	4.542	1.00
70_V	256_E	3.708	1.00
17_V	73_A	3.555	1.00
35_D	39_Y	3.288	1.00
168_N	338_G	3.269	1.00
74_P	252_D	3.049	1.00
26_E	94_R	3.025	1.00
337_S	340_Q	2.963	1.00
331_D	335_D	2.954	1.00
239_V	258_A	2.932	1.00
14_T	141_G	2.876	1.00
69_G	72_E	2.860	1.00
68_N	72_E	2.854	1.00
67_L	72_E	2.829	1.00
166_S	254_F	2.728	1.00
113_E	118_R	2.665	1.00
345_I	349_H	2.654	1.00
180_A	340_Q	2.570	1.00
318_D	330_R	2.544	1.00
180_A	343_R	2.520	1.00
145_P	206_Q	2.456	1.00
210_R	214_E	2.408	1.00
171_Y	346_L	2.379	1.00
148_A	230_P	2.327	1.00
299_D	340_Q	2.265	1.00
201_S	204_A	2.255	1.00
200_E	204_A	2.220	1.00
97_G	244_A	2.199	1.00
264_E	323_R	2.130	1.00
312_D	315_T	2.084	1.00
292_R	296_D	2.068	1.00
74_P	78_R	2.058	1.00
330_R	334_D	2.026	1.00
25_D	28_G	2.000	1.00
167_A	337_S	1.989	1.00
245_Q	254_F	1.978	1.00
212_M	228_V	1.916	1.00
155_M	239_V	1.913	1.00
257_Y	324_L	1.908	1.00
340_Q	344_R	1.900	1.00
78_R	82_A	1.876	1.00
268_R	273_P	1.840	1.00
247_D	250_V	1.780	1.00
152_S	237_V	1.777	1.00
51_L	84_L	1.758	1.00
338_G	342_Q	1.744	1.00
13_G	263_T	1.718	1.00
311_V	316_V	1.687	1.00
23_V	93_L	1.679	1.00
27_H	92_G	1.660	1.00
64_T	67_L	1.649	1.00
150_W	269_Y	1.642	1.00
180_A	339_A	1.641	1.00
158_H	261_L	1.641	1.00
299_D	314_G	1.629	1.00
22_F	134_A	1.626	1.00
67_L	76_A	1.624	1.00
24_V	96_A	1.610	1.00
318_D	322_D	1.581	1.00
16_G	20_E	1.540	1.00
35_D	41_G	1.537	1.00
74_P	249_A	1.529	1.00
77_I	248_P	1.523	1.00
185_K	188_E	1.520	1.00
52_D	63_Q	1.517	1.00
263_T	267_Q	1.494	1.00
85_V	94_R	1.490	1.00
34_S	37_L	1.488	1.00
35_D	38_V	1.481	1.00
144_D	147_K	1.479	1.00
246_A	338_G	1.474	1.00
125_Q	129_H	1.466	1.00
332_V	336_V	1.462	1.00
71_A	75_A	1.461	1.00
150_W	272_H	1.455	1.00
344_R	348_T	1.452	1.00
101_H	104_A	1.452	1.00
101_H	109_H	1.439	1.00
327_S	330_R	1.420	1.00
301_S	310_A	1.415	1.00
30_P	37_L	1.414	1.00
17_V	255_V	1.410	1.00
311_V	319_R	1.403	1.00
202_Y	206_Q	1.400	1.00
322_D	325_G	1.398	1.00
26_E	89_S	1.378	1.00
292_R	300_A	1.367	1.00
253_A	329_I	1.365	1.00
116_R	220_D	1.362	1.00
37_L	42_E	1.362	1.00
305_R	309_G	1.361	1.00
282_R	285_L	1.358	1.00
105_R	108_E	1.358	1.00
161_V	317_V	1.354	1.00
152_S	230_P	1.353	1.00
100_L	172_W	1.351	1.00
316_V	320_E	1.338	1.00
139_H	236_T	1.332	1.00
328_G	331_D	1.329	1.00
99_G	244_A	1.322	1.00
136_L	251_V	1.320	1.00
317_V	321_C	1.316	1.00
60_V	84_L	1.315	1.00
30_P	104_A	1.312	1.00
288_A	292_R	1.311	1.00
321_C	326_V	1.302	1.00
75_A	79_E	1.301	1.00
253_A	328_G	1.292	0.99
191_P	194_G	1.283	0.99
83_A	86_A	1.281	0.99
37_L	41_G	1.267	0.99
171_Y	343_R	1.265	0.99
315_T	318_D	1.258	0.99
78_R	248_P	1.258	0.99
30_P	33_G	1.257	0.99
272_H	278_V	1.256	0.99
204_A	207_A	1.254	0.99
143_D	147_K	1.248	0.99
250_V	341_Q	1.247	0.99
115_P	118_R	1.243	0.99
29_V	33_G	1.217	0.99
179_L	185_K	1.212	0.99
172_W	177_T	1.206	0.99
146_D	202_Y	1.202	0.99
314_G	333_Y	1.195	0.99
66_T	141_G	1.195	0.99
320_E	324_L	1.188	0.99
22_F	59_V	1.186	0.99
249_A	328_G	1.175	0.99
225_W	229_R	1.172	0.99
292_R	310_A	1.160	0.99
155_M	262_V	1.154	0.99
72_E	76_A	1.149	0.99
138_I	255_V	1.149	0.99
346_L	350_G	1.144	0.99
130_R	189_G	1.124	0.99
318_D	333_Y	1.114	0.98
179_L	294_M	1.111	0.98
153_N	200_E	1.107	0.98
319_R	323_R	1.089	0.98
264_E	267_Q	1.088	0.98
21_F	62_T	1.083	0.98
301_S	312_D	1.077	0.98
222_G	225_W	1.074	0.98
348_T	351_E	1.071	0.98
162_L	258_A	1.070	0.98
179_L	295_R	1.069	0.98
26_E	92_G	1.069	0.98
322_D	347_D	1.068	0.98
82_A	86_A	1.066	0.98
70_V	252_D	1.058	0.98
39_Y	42_E	1.058	0.98
111_H	121_L	1.051	0.98
40_E	91_H	1.049	0.98
14_T	66_T	1.045	0.98
163_L	166_S	1.045	0.98
302_F	313_L	1.042	0.98
180_A	297_G	1.042	0.98
151_V	262_V	1.030	0.97
57_K	113_E	1.025	0.97
346_L	349_H	1.017	0.97
75_A	78_R	1.004	0.97
33_G	39_Y	0.997	0.97
77_I	252_D	0.991	0.97
285_L	303_V	0.990	0.97
205_F	209_E	0.988	0.97
46_P	83_A	0.985	0.97
61_E	240_R	0.975	0.96
163_L	184_A	0.972	0.96
81_R	84_L	0.964	0.96
154_R	268_R	0.955	0.96
203_A	207_A	0.955	0.96
124_I	187_F	0.952	0.96
208_F	211_R	0.947	0.96
321_C	329_I	0.945	0.96
31_T	38_V	0.941	0.96
345_I	348_T	0.940	0.96
23_V	95_I	0.935	0.95
207_A	211_R	0.930	0.95
171_Y	176_D	0.929	0.95
30_P	39_Y	0.929	0.95
180_A	299_D	0.928	0.95
154_R	265_Y	0.927	0.95
155_M	258_A	0.925	0.95
172_W	186_I	0.925	0.95
22_F	99_G	0.924	0.95
163_L	241_A	0.920	0.95
287_D	291_W	0.916	0.95
15_L	140_V	0.916	0.95
133_T	187_F	0.914	0.95
166_S	242_P	0.910	0.95
155_M	237_V	0.909	0.95
147_K	266_A	0.907	0.95
97_G	248_P	0.906	0.95
176_D	180_A	0.906	0.95
30_P	34_S	0.903	0.94
211_R	215_H	0.899	0.94
112_A	117_Y	0.897	0.94
269_Y	272_H	0.890	0.94
147_K	150_W	0.884	0.94
150_W	154_R	0.880	0.94
98_A	168_N	0.880	0.94
176_D	347_D	0.880	0.94
161_V	307_T	0.873	0.93
41_G	90_E	0.869	0.93
87_Y	91_H	0.868	0.93
343_R	347_D	0.864	0.93
151_V	237_V	0.860	0.93
142_V	237_V	0.855	0.93
140_V	259_H	0.852	0.93
162_L	242_P	0.848	0.92
347_D	350_G	0.848	0.92
188_E	287_D	0.847	0.92
344_R	347_D	0.846	0.92
50_R	136_L	0.844	0.92
20_E	61_E	0.844	0.92
321_C	333_Y	0.842	0.92
198_A	201_S	0.842	0.92
317_V	320_E	0.841	0.92
314_G	318_D	0.839	0.92
86_A	90_E	0.835	0.92
342_Q	345_I	0.831	0.92
162_L	239_V	0.829	0.92
74_P	248_P	0.828	0.92
127_P	130_R	0.822	0.91
165_L	332_V	0.819	0.91
264_E	268_R	0.818	0.91
200_E	207_A	0.816	0.91
16_G	61_E	0.814	0.91
36_E	39_Y	0.812	0.91
167_A	180_A	0.812	0.91
98_A	342_Q	0.808	0.91
24_V	94_R	0.806	0.90
50_R	65_P	0.804	0.90
199_F	205_F	0.803	0.90
181_S	243_D	0.801	0.90
118_R	122_D	0.801	0.90
136_L	244_A	0.799	0.90
178_G	181_S	0.795	0.90
291_W	295_R	0.794	0.90
164_A	293_A	0.792	0.90
32_A	37_L	0.787	0.89
260_A	324_L	0.786	0.89
33_G	37_L	0.786	0.89
58_F	132_T	0.781	0.89
335_D	341_Q	0.779	0.89
203_A	206_Q	0.778	0.89
296_D	300_A	0.775	0.89
151_V	266_A	0.774	0.89
41_G	91_H	0.770	0.88
227_D	290_K	0.769	0.88
111_H	118_R	0.768	0.88
317_V	333_Y	0.766	0.88
242_P	254_F	0.765	0.88
160_P	243_D	0.764	0.88
19_E	81_R	0.763	0.88
331_D	334_D	0.761	0.88
163_L	167_A	0.760	0.88
56_F	133_T	0.759	0.88
119_S	122_D	0.754	0.87
55_L	120_Q	0.753	0.87
37_L	91_H	0.752	0.87
153_N	198_A	0.751	0.87
321_C	324_L	0.747	0.87
316_V	321_C	0.747	0.87
164_A	298_H	0.746	0.87
138_I	239_V	0.742	0.86
77_I	136_L	0.736	0.86
36_E	40_E	0.735	0.86
38_V	42_E	0.732	0.86
15_L	73_A	0.731	0.86
298_H	333_Y	0.730	0.85
56_F	132_T	0.728	0.85
61_E	137_H	0.728	0.85
131_N	187_F	0.725	0.85
62_T	84_L	0.721	0.85
306_E	309_G	0.717	0.84
143_D	266_A	0.716	0.84
223_E	227_D	0.713	0.84
283_R	286_L	0.712	0.84
326_V	332_V	0.712	0.84
130_R	225_W	0.710	0.84
245_Q	338_G	0.710	0.84
33_G	93_L	0.708	0.84
316_V	322_D	0.706	0.84
67_L	74_P	0.706	0.84
152_S	228_V	0.705	0.83
221_R	232_S	0.704	0.83
20_E	240_R	0.704	0.83
90_E	344_R	0.702	0.83
127_P	185_K	0.702	0.83
50_R	62_T	0.701	0.83
138_I	242_P	0.700	0.83
250_V	338_G	0.700	0.83
126_Y	129_H	0.699	0.83
319_R	330_R	0.699	0.83
29_V	32_A	0.694	0.82
148_A	205_F	0.692	0.82
75_A	86_A	0.691	0.82
222_G	232_S	0.690	0.82
159_M	239_V	0.690	0.82
182_A	186_I	0.687	0.82
55_L	112_A	0.685	0.82
128_Q	131_N	0.684	0.82
184_A	294_M	0.684	0.82
15_L	70_V	0.682	0.81
15_L	259_H	0.681	0.81
127_P	188_E	0.680	0.81
99_G	261_L	0.679	0.81
30_P	101_H	0.679	0.81
138_I	259_H	0.677	0.81
228_V	237_V	0.676	0.81
149_V	153_N	0.674	0.81
341_Q	345_I	0.672	0.80
59_V	124_I	0.672	0.80
248_P	252_D	0.671	0.80
70_V	261_L	0.670	0.80
295_R	299_D	0.669	0.80
304_A	319_R	0.667	0.80
72_E	334_D	0.666	0.80
29_V	108_E	0.664	0.80
130_R	186_I	0.664	0.80
265_Y	269_Y	0.662	0.79
249_A	252_D	0.661	0.79
220_D	223_E	0.661	0.79
153_N	204_A	0.659	0.79
70_V	259_H	0.657	0.79
257_Y	320_E	0.657	0.79
152_S	156_R	0.651	0.78
280_G	329_I	0.649	0.78
54_E	61_E	0.649	0.78
57_K	112_A	0.648	0.78
230_P	246_A	0.648	0.78
308_Q	333_Y	0.647	0.78
33_G	36_E	0.647	0.78
164_A	167_A	0.647	0.78
332_V	338_G	0.646	0.78
57_K	110_E	0.646	0.78
168_N	260_A	0.646	0.78
185_K	294_M	0.643	0.77
57_K	109_H	0.642	0.77
137_H	236_T	0.641	0.77
195_L	198_A	0.640	0.77
77_I	81_R	0.639	0.77
125_Q	296_D	0.638	0.77
163_L	290_K	0.634	0.76
187_F	227_D	0.632	0.76
257_Y	317_V	0.632	0.76
139_H	229_R	0.632	0.76
188_E	291_W	0.630	0.76
46_P	49_G	0.629	0.76
329_I	333_Y	0.627	0.76
97_G	245_Q	0.622	0.75
314_G	334_D	0.619	0.75
156_R	286_L	0.618	0.75
79_E	82_A	0.616	0.74
170_P	181_S	0.616	0.74
67_L	73_A	0.616	0.74
34_S	38_V	0.615	0.74
311_V	315_T	0.614	0.74
61_E	238_E	0.613	0.74
179_L	225_W	0.612	0.74
23_V	88_A	0.612	0.74
220_D	231_H	0.611	0.74
52_D	114_K	0.610	0.74
30_P	103_G	0.609	0.74
300_A	305_R	0.609	0.74
177_T	182_A	0.608	0.73
68_N	175_F	0.606	0.73
289_N	302_F	0.605	0.73
131_N	190_L	0.605	0.73
62_T	80_V	0.601	0.73
34_S	93_L	0.599	0.72
213_V	220_D	0.598	0.72
346_L	351_E	0.598	0.72
33_G	57_K	0.596	0.72
17_V	135_G	0.594	0.72
21_F	95_I	0.594	0.72
293_A	302_F	0.594	0.72
21_F	84_L	0.593	0.72
286_L	289_N	0.593	0.72
136_L	245_Q	0.592	0.71
77_I	251_V	0.591	0.71
293_A	300_A	0.591	0.71
116_R	120_Q	0.590	0.71
212_M	288_A	0.588	0.71
126_Y	192_N	0.588	0.71
119_S	123_R	0.587	0.71
137_H	246_A	0.585	0.71
145_P	232_S	0.585	0.71
248_P	317_V	0.584	0.70
14_T	68_N	0.582	0.70
195_L	287_D	0.581	0.70
330_R	333_Y	0.580	0.70
180_A	337_S	0.578	0.70
104_A	132_T	0.578	0.70
75_A	82_A	0.576	0.69
22_F	96_A	0.575	0.69
146_D	347_D	0.574	0.69
179_L	189_G	0.572	0.69
229_R	236_T	0.570	0.69
256_E	326_V	0.570	0.69
64_T	139_H	0.568	0.68
35_D	40_E	0.567	0.68
181_S	184_A	0.567	0.68
273_P	278_V	0.567	0.68
15_L	64_T	0.566	0.68
327_S	331_D	0.566	0.68
55_L	130_R	0.565	0.68
56_F	124_I	0.564	0.68
25_D	29_V	0.563	0.68
220_D	224_L	0.563	0.68
187_F	193_T	0.562	0.68
12_S	86_A	0.561	0.67
49_G	119_S	0.561	0.67
213_V	216_G	0.560	0.67
116_R	261_L	0.560	0.67
81_R	85_V	0.559	0.67
50_R	80_V	0.559	0.67
156_R	195_L	0.559	0.67
141_G	187_F	0.558	0.67
17_V	64_T	0.558	0.67
66_T	248_P	0.554	0.66
79_E	327_S	0.554	0.66
130_R	172_W	0.553	0.66
180_A	341_Q	0.552	0.66
117_Y	121_L	0.552	0.66
161_V	313_L	0.552	0.66
165_L	311_V	0.552	0.66
28_G	97_G	0.552	0.66
24_V	237_V	0.552	0.66
262_V	266_A	0.551	0.66
132_T	187_F	0.551	0.66
225_W	231_H	0.551	0.66
38_V	41_G	0.551	0.66
49_G	115_P	0.548	0.66
150_W	268_R	0.547	0.65
100_L	182_A	0.547	0.65
117_Y	120_Q	0.545	0.65
226_Y	229_R	0.544	0.65
316_V	319_R	0.542	0.65
100_L	186_I	0.542	0.65
301_S	305_R	0.541	0.65
57_K	63_Q	0.540	0.64
143_D	270_D	0.539	0.64
163_L	293_A	0.537	0.64
125_Q	178_G	0.537	0.64
207_A	210_R	0.536	0.64
108_E	122_D	0.536	0.64
13_G	140_V	0.535	0.64
130_R	190_L	0.534	0.64
25_D	38_V	0.533	0.63
260_A	321_C	0.531	0.63
324_L	348_T	0.530	0.63
197_T	200_E	0.530	0.63
326_V	329_I	0.529	0.63
147_K	270_D	0.527	0.63
55_L	208_F	0.526	0.62
184_A	187_F	0.524	0.62
59_V	133_T	0.523	0.62
252_D	255_V	0.523	0.62
102_P	177_T	0.521	0.62
315_T	319_R	0.521	0.62
311_V	314_G	0.520	0.62
115_P	119_S	0.519	0.61
162_L	257_Y	0.518	0.61
39_Y	51_L	0.518	0.61
95_I	346_L	0.518	0.61
32_A	93_L	0.518	0.61
303_V	310_A	0.517	0.61
180_A	336_V	0.517	0.61
64_T	76_A	0.517	0.61
32_A	39_Y	0.517	0.61
58_F	131_N	0.516	0.61
143_D	263_T	0.515	0.61
60_V	82_A	0.515	0.61
347_D	351_E	0.514	0.61
284_E	288_A	0.514	0.61
322_D	331_D	0.513	0.61
101_H	302_F	0.513	0.61
179_L	182_A	0.513	0.61
228_V	234_H	0.512	0.60
192_N	212_M	0.512	0.60
31_T	42_E	0.511	0.60
106_W	109_H	0.510	0.60
338_G	341_Q	0.508	0.60
59_V	134_A	0.508	0.60
149_V	212_M	0.506	0.60
58_F	80_V	0.506	0.60
64_T	155_M	0.505	0.59
197_T	283_R	0.505	0.59
73_A	252_D	0.505	0.59
165_L	204_A	0.505	0.59
135_G	139_H	0.504	0.59
137_H	244_A	0.504	0.59
322_D	330_R	0.504	0.59
56_F	111_H	0.503	0.59
310_A	313_L	0.503	0.59
245_Q	251_V	0.501	0.59
55_L	187_F	0.500	0.59

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1r8gA	2	0.95	100	0.237	Contact Map
2gwdA	2	0.9722	100	0.263	Contact Map
3nztA	1	0.9472	100	0.4	Contact Map
1va6A	1	0.9417	100	0.416	Contact Map
3ln6A	2	0.9222	100	0.425	Contact Map
3ln7A	2	0.9306	100	0.429	Contact Map
3ig5A	1	0.9806	100	0.507	Contact Map
4hppA	3	0.7722	99	0.867	Contact Map
3fkyA	8	0.5611	98.3	0.895	Contact Map
4baxA	5	0.5694	98.3	0.895	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0217 seconds.