Q9HPP4 - HALSA - GREMLIN Contacts

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Q9HPP4 - Uncharacterized protein

UniProt:	Q9HPP4	Sequences: MTTTIKDSVHDHIEVTGVVAALLDTPPLQRLRNVAQLGTVSLVYPSANHTRFEHSLGVYHLADRALDHLGITGQRATRVRAAAILHDIGHGPFSHNVEDVIERHTGNRHDDVHDLVASGQVASVLADHDIQPAEIAALVAGDGEPLAQLVAGELDVDRMDYLVRDAHHTGVPYGTIDHGRLIRGLTFIDGDLVLGAGNVQSAESLLLARALMNPTVYSHHVARISKSMLRRAAETLLATTDVTAPALRRMDDHDLVAALRAHDATSRVAARLFERDLYKRAVWAELGDVDDDLVGADHDTVRGFERAIAADAGVPERDVIVDAPSEPSMPESSTGVLVNGEVRRLHEQSTLVTALQTAQREQWRLGVYTPRAHTDVVGRATERVLGLDPDDGLVTEVNAPDDSPPGFSE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	409 (364)
Sequences:	1294 (926)
Seq/√Len:	48.5

BACSU - YWFO YDHJ
HALSA - Q9HPP4 Q9HMK5 Q9HSK2
SULSO - Q97XC0 Q97U95 Q7LXZ1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
19_V	80_R	3.855	1.00
118_S	136_A	3.769	1.00
162_L	212_M	3.681	1.00
29_Q	32_R	3.530	1.00
65_A	156_V	3.469	1.00
33_N	251_D	3.390	1.00
267_R	271_R	3.232	1.00
111_D	115_L	3.212	1.00
22_L	81_A	3.072	1.00
265_T	268_V	2.969	1.00
85_L	88_I	2.958	1.00
63_D	80_R	2.892	1.00
25_T	124_V	2.839	1.00
32_R	52_F	2.811	1.00
61_L	156_V	2.793	1.00
67_D	76_A	2.725	1.00
8_S	46_S	2.539	1.00
212_M	217_Y	2.509	1.00
232_A	269_A	2.485	1.00
180_R	219_H	2.441	1.00
271_R	276_D	2.392	1.00
133_A	137_A	2.346	1.00
62_A	83_A	2.318	1.00
120_Q	124_V	2.305	1.00
40_V	222_A	2.268	1.00
212_M	216_V	2.242	1.00
62_A	79_V	2.207	1.00
318_D	321_V	2.202	1.00
135_I	139_V	2.160	1.00
91_G	97_V	2.153	1.00
318_D	323_A	2.146	1.00
136_A	139_V	2.141	1.00
185_L	194_L	2.000	0.99
122_A	126_A	1.905	0.99
19_V	59_Y	1.877	0.99
5_I	45_P	1.859	0.99
120_Q	125_L	1.818	0.99
93_F	256_V	1.816	0.99
316_E	321_V	1.810	0.99
85_L	117_A	1.798	0.99
25_T	28_L	1.797	0.99
275_R	279_K	1.777	0.99
90_H	110_D	1.736	0.99
180_R	275_R	1.707	0.98
121_V	125_L	1.704	0.98
317_R	321_V	1.684	0.98
321_V	324_P	1.643	0.98
132_P	136_A	1.615	0.98
15_V	20_A	1.614	0.98
59_Y	80_R	1.598	0.97
86_H	157_D	1.595	0.97
169_T	216_V	1.595	0.97
21_A	127_D	1.593	0.97
135_I	138_L	1.588	0.97
42_L	225_S	1.587	0.97
225_S	277_L	1.587	0.97
26_P	120_Q	1.587	0.97
114_D	140_A	1.576	0.97
266_S	269_A	1.573	0.97
235_T	264_A	1.564	0.97
3_T	20_A	1.563	0.97
11_D	179_G	1.563	0.97
121_V	124_V	1.550	0.97
24_D	32_R	1.543	0.97
194_L	202_A	1.542	0.97
318_D	324_P	1.538	0.97
95_H	157_D	1.530	0.97
10_H	163_V	1.529	0.97
309_A	313_G	1.524	0.97
198_N	201_S	1.519	0.97
316_E	323_A	1.506	0.96
162_L	208_A	1.497	0.96
75_R	143_G	1.491	0.96
305_E	308_I	1.483	0.96
62_A	149_L	1.481	0.96
5_I	15_V	1.480	0.96
79_V	150_V	1.476	0.96
23_L	28_L	1.475	0.96
66_L	80_R	1.471	0.96
232_A	259_L	1.470	0.96
228_M	277_L	1.465	0.96
345_L	351_L	1.440	0.95
126_A	129_D	1.435	0.95
6_K	12_H	1.433	0.95
297_D	301_V	1.432	0.95
65_A	185_L	1.423	0.95
87_D	157_D	1.417	0.95
320_I	324_P	1.414	0.95
86_H	109_H	1.413	0.95
228_M	278_Y	1.408	0.95
155_D	158_R	1.406	0.95
243_T	246_A	1.395	0.94
306_R	310_A	1.395	0.94
163_V	178_H	1.392	0.94
8_S	167_H	1.392	0.94
58_V	156_V	1.386	0.94
79_V	146_L	1.380	0.94
223_R	322_D	1.376	0.94
110_D	157_D	1.374	0.94
116_V	248_R	1.365	0.94
9_V	167_H	1.359	0.94
220_H	280_R	1.349	0.93
109_H	157_D	1.342	0.93
271_R	278_Y	1.340	0.93
58_V	83_A	1.324	0.93
51_R	54_H	1.322	0.93
146_L	150_V	1.313	0.92
283_W	366_G	1.311	0.92
329_M	332_S	1.309	0.92
90_H	109_H	1.304	0.92
219_H	275_R	1.298	0.92
41_S	50_T	1.295	0.92
345_L	349_S	1.294	0.92
171_V	212_M	1.284	0.91
188_I	193_V	1.277	0.91
9_V	13_I	1.268	0.91
22_L	85_L	1.257	0.91
305_E	309_A	1.256	0.91
130_I	134_E	1.253	0.90
173_Y	212_M	1.252	0.90
48_N	53_E	1.243	0.90
6_K	210_A	1.242	0.90
60_H	63_D	1.235	0.90
301_V	304_F	1.215	0.89
316_E	320_I	1.212	0.89
135_I	141_G	1.212	0.89
60_H	64_R	1.207	0.89
171_V	174_G	1.201	0.88
313_G	316_E	1.200	0.88
357_T	360_R	1.199	0.88
221_V	275_R	1.192	0.88
228_M	232_A	1.171	0.87
300_T	304_F	1.168	0.87
68_H	182_I	1.166	0.87
69_L	92_P	1.159	0.87
32_R	42_L	1.156	0.86
235_T	239_T	1.154	0.86
134_E	143_G	1.140	0.86
126_A	130_I	1.139	0.86
176_I	206_L	1.130	0.85
298_H	301_V	1.130	0.85
50_T	88_I	1.129	0.85
298_H	302_R	1.126	0.85
88_I	117_A	1.122	0.85
51_R	57_G	1.118	0.85
314_V	321_V	1.114	0.84
302_R	306_R	1.109	0.84
86_H	110_D	1.106	0.84
352_V	355_L	1.105	0.84
225_S	268_V	1.100	0.84
89_G	94_S	1.100	0.84
270_A	274_E	1.094	0.83
265_T	269_A	1.093	0.83
123_S	127_D	1.092	0.83
246_A	249_R	1.091	0.83
320_I	323_A	1.089	0.83
310_A	313_G	1.088	0.83
317_R	320_I	1.087	0.83
95_H	110_D	1.086	0.83
29_Q	52_F	1.081	0.82
316_E	319_V	1.078	0.82
270_A	276_D	1.073	0.82
94_S	158_R	1.070	0.82
110_D	113_H	1.070	0.82
158_R	206_L	1.064	0.81
269_A	273_F	1.064	0.81
131_Q	139_V	1.062	0.81
29_Q	93_F	1.055	0.81
16_T	19_V	1.054	0.81
316_E	324_P	1.050	0.81
208_A	212_M	1.047	0.80
350_T	359_Q	1.042	0.80
64_R	179_G	1.036	0.80
304_F	311_D	1.034	0.80
224_I	278_Y	1.032	0.79
132_P	135_I	1.030	0.79
37_L	169_T	1.030	0.79
158_R	209_R	1.027	0.79
24_D	29_Q	1.027	0.79
95_H	109_H	1.024	0.79
61_L	178_H	1.023	0.79
221_V	279_K	1.022	0.79
38_G	155_D	1.022	0.79
303_G	308_I	1.017	0.78
15_V	19_V	1.017	0.78
87_D	109_H	1.015	0.78
133_A	270_A	1.014	0.78
38_G	94_S	1.013	0.78
232_A	268_V	1.012	0.78
67_D	74_Q	1.009	0.78
213_N	218_S	1.009	0.78
162_L	216_V	1.004	0.78
266_S	273_F	1.004	0.78
87_D	95_H	1.003	0.77
126_A	132_P	0.995	0.77
347_E	353_T	0.994	0.77
4_T	12_H	0.992	0.77
165_D	217_Y	0.991	0.77
47_A	169_T	0.989	0.76
266_S	270_A	0.986	0.76
7_D	10_H	0.986	0.76
341_E	347_E	0.985	0.76
196_A	338_V	0.985	0.76
68_H	183_R	0.983	0.76
309_A	321_V	0.982	0.76
9_V	119_G	0.981	0.76
54_H	57_G	0.977	0.76
38_G	226_K	0.976	0.75
131_Q	134_E	0.970	0.75
364_R	368_Y	0.968	0.75
300_T	303_G	0.958	0.74
163_V	167_H	0.954	0.74
134_E	138_L	0.953	0.74
36_Q	49_H	0.946	0.73
52_F	180_R	0.945	0.73
184_G	198_N	0.940	0.73
343_R	348_Q	0.938	0.73
96_N	222_A	0.926	0.72
8_S	168_H	0.925	0.71
24_D	275_R	0.920	0.71
24_D	219_H	0.919	0.71
8_S	170_G	0.915	0.71
299_D	304_F	0.912	0.70
159_M	176_I	0.910	0.70
88_I	347_E	0.910	0.70
50_T	311_D	0.902	0.70
46_S	168_H	0.901	0.69
118_S	133_A	0.900	0.69
15_V	56_L	0.897	0.69
91_G	250_M	0.887	0.68
91_G	247_L	0.886	0.68
147_A	150_V	0.886	0.68
319_V	322_D	0.885	0.68
158_R	207_L	0.883	0.68
364_R	367_V	0.883	0.68
228_M	268_V	0.883	0.68
53_E	168_H	0.882	0.68
313_G	319_V	0.881	0.68
72_T	143_G	0.881	0.68
94_S	226_K	0.878	0.67
349_S	353_T	0.874	0.67
119_G	125_L	0.870	0.67
43_V	79_V	0.870	0.67
20_A	24_D	0.866	0.66
86_H	95_H	0.866	0.66
174_G	212_M	0.862	0.66
299_D	303_G	0.859	0.66
299_D	310_A	0.857	0.66
174_G	203_E	0.857	0.66
29_Q	219_H	0.857	0.66
62_A	156_V	0.855	0.65
306_R	309_A	0.854	0.65
78_R	134_E	0.854	0.65
72_T	75_R	0.850	0.65
49_H	165_D	0.848	0.65
214_P	251_D	0.847	0.65
103_R	234_E	0.843	0.64
142_D	145_P	0.842	0.64
203_E	212_M	0.842	0.64
53_E	180_R	0.841	0.64
315_P	334_T	0.839	0.64
361_E	364_R	0.838	0.64
344_R	347_E	0.838	0.64
98_E	203_E	0.835	0.64
14_E	183_R	0.835	0.64
5_I	9_V	0.834	0.63
167_H	213_N	0.834	0.63
279_K	282_V	0.832	0.63
5_I	23_L	0.832	0.63
27_P	83_A	0.831	0.63
162_L	166_A	0.830	0.63
254_D	258_A	0.828	0.63
6_K	44_Y	0.821	0.62
74_Q	134_E	0.819	0.62
110_D	161_Y	0.817	0.62
100_V	116_V	0.816	0.62
207_L	211_L	0.816	0.62
301_V	306_R	0.814	0.62
90_H	157_D	0.813	0.61
152_G	185_L	0.812	0.61
94_S	209_R	0.812	0.61
19_V	63_D	0.811	0.61
294_V	298_H	0.809	0.61
46_S	195_G	0.806	0.61
64_R	67_D	0.805	0.61
43_V	112_V	0.804	0.61
86_H	90_H	0.802	0.60
233_A	319_V	0.801	0.60
309_A	314_V	0.798	0.60
90_H	161_Y	0.794	0.60
33_N	274_E	0.794	0.60
154_L	176_I	0.792	0.59
365_L	369_T	0.792	0.59
326_E	331_E	0.791	0.59
124_V	128_H	0.789	0.59
123_S	126_A	0.784	0.59
39_T	229_L	0.782	0.58
338_V	343_R	0.781	0.58
173_Y	210_A	0.781	0.58
296_A	303_G	0.780	0.58
31_L	36_Q	0.779	0.58
317_R	323_A	0.776	0.58
231_R	234_E	0.773	0.58
98_E	207_L	0.770	0.57
39_T	226_K	0.770	0.57
276_D	279_K	0.768	0.57
23_L	26_P	0.768	0.57
8_S	13_I	0.765	0.57
339_N	351_L	0.765	0.57
308_I	311_D	0.764	0.57
131_Q	141_G	0.762	0.56
255_L	272_L	0.761	0.56
233_A	247_L	0.760	0.56
162_L	171_V	0.757	0.56
40_V	44_Y	0.755	0.56
315_P	319_V	0.748	0.55
266_S	279_K	0.747	0.55
355_L	358_A	0.746	0.55
125_L	128_H	0.745	0.55
150_V	175_T	0.744	0.55
360_R	365_L	0.739	0.54
332_S	336_V	0.734	0.54
130_I	135_I	0.734	0.54
306_R	313_G	0.733	0.54
121_V	126_A	0.731	0.53
28_L	186_T	0.729	0.53
112_V	247_L	0.729	0.53
127_D	270_A	0.728	0.53
154_L	205_L	0.725	0.53
56_L	202_A	0.725	0.53
3_T	11_D	0.725	0.53
200_Q	215_T	0.724	0.53
19_V	81_A	0.723	0.53
9_V	46_S	0.722	0.52
87_D	110_D	0.722	0.52
95_H	161_Y	0.721	0.52
186_T	193_V	0.719	0.52
22_L	121_V	0.719	0.52
45_P	48_N	0.718	0.52
172_P	175_T	0.718	0.52
305_E	310_A	0.717	0.52
190_G	234_E	0.717	0.52
180_R	352_V	0.716	0.52
168_H	222_A	0.715	0.52
151_A	155_D	0.710	0.51
149_L	193_V	0.710	0.51
144_E	291_D	0.708	0.51
308_I	317_R	0.706	0.51
311_D	318_D	0.703	0.51
37_L	47_A	0.702	0.50
131_Q	135_I	0.699	0.50
315_P	321_V	0.697	0.50
269_A	278_Y	0.694	0.50
319_V	324_P	0.694	0.50
297_D	304_F	0.692	0.49
194_L	198_N	0.691	0.49
58_V	154_L	0.690	0.49
98_E	345_L	0.689	0.49
66_L	79_V	0.687	0.49
326_E	330_P	0.687	0.49
52_F	219_H	0.686	0.49
200_Q	210_A	0.686	0.49
180_R	221_V	0.685	0.49
31_L	55_S	0.685	0.49
315_P	323_A	0.682	0.48
45_P	53_E	0.680	0.48
180_R	216_V	0.679	0.48
153_E	158_R	0.678	0.48
134_E	139_V	0.674	0.48
88_I	113_H	0.673	0.48
43_V	47_A	0.672	0.47
299_D	306_R	0.671	0.47
38_G	161_Y	0.670	0.47
197_G	219_H	0.669	0.47
68_H	178_H	0.663	0.47
265_T	273_F	0.660	0.46
169_T	204_S	0.660	0.46
263_D	321_V	0.659	0.46
152_G	206_L	0.659	0.46
119_G	248_R	0.659	0.46
338_V	341_E	0.657	0.46
90_H	95_H	0.657	0.46
132_P	139_V	0.656	0.46
292_D	299_D	0.656	0.46
297_D	300_T	0.656	0.46
141_G	151_A	0.653	0.46
334_T	338_V	0.652	0.45
236_L	262_H	0.652	0.45
38_G	222_A	0.651	0.45
356_Q	361_E	0.650	0.45
92_P	256_V	0.649	0.45
43_V	219_H	0.648	0.45
116_V	244_A	0.646	0.45
306_R	311_D	0.644	0.45
130_I	139_V	0.644	0.45
224_I	277_L	0.644	0.45
301_V	305_E	0.640	0.44
267_R	276_D	0.638	0.44
14_E	224_I	0.637	0.44
203_E	207_L	0.634	0.44
237_L	240_T	0.634	0.44
195_G	280_R	0.634	0.44
96_N	226_K	0.633	0.44
159_M	183_R	0.629	0.43
97_V	156_V	0.628	0.43
260_R	273_F	0.628	0.43
172_P	181_L	0.628	0.43
101_I	247_L	0.627	0.43
12_H	56_L	0.627	0.43
91_G	217_Y	0.627	0.43
78_R	82_A	0.627	0.43
232_A	272_L	0.625	0.43
37_L	202_A	0.624	0.43
36_Q	165_D	0.624	0.43
150_V	180_R	0.623	0.43
330_P	360_R	0.623	0.43
12_H	255_L	0.621	0.42
10_H	209_R	0.620	0.42
111_D	140_A	0.616	0.42
112_V	222_A	0.616	0.42
309_A	319_V	0.616	0.42
222_A	271_R	0.615	0.42
201_S	215_T	0.614	0.42
269_A	276_D	0.614	0.42
88_I	353_T	0.613	0.42
46_S	252_D	0.611	0.41
19_V	156_V	0.609	0.41
33_N	253_H	0.609	0.41
232_A	265_T	0.608	0.41
268_V	285_E	0.607	0.41
235_T	238_A	0.607	0.41
151_A	158_R	0.606	0.41
366_G	369_T	0.606	0.41
200_Q	214_P	0.604	0.41
173_Y	208_A	0.604	0.41
288_D	314_V	0.603	0.41
24_D	279_K	0.602	0.40
169_T	176_I	0.598	0.40
228_M	265_T	0.597	0.40
4_T	16_T	0.593	0.40
249_R	260_R	0.590	0.39
295_G	300_T	0.589	0.39
136_A	197_G	0.588	0.39
66_L	76_A	0.587	0.39
296_A	300_T	0.587	0.39
360_R	363_W	0.586	0.39
208_A	356_Q	0.581	0.38
35_A	252_D	0.580	0.38
24_D	65_A	0.579	0.38
350_T	355_L	0.579	0.38
272_L	275_R	0.578	0.38
137_A	147_A	0.577	0.38
314_V	318_D	0.577	0.38
159_M	205_L	0.577	0.38
17_G	23_L	0.577	0.38
26_P	274_E	0.576	0.38
44_Y	225_S	0.575	0.38
159_M	163_V	0.574	0.38
94_S	155_D	0.574	0.38
10_H	32_R	0.573	0.38
162_L	173_Y	0.573	0.38
90_H	165_D	0.572	0.38
6_K	166_A	0.570	0.37
101_I	278_Y	0.570	0.37
161_Y	216_V	0.569	0.37
171_V	198_N	0.568	0.37
96_N	156_V	0.568	0.37
45_P	49_H	0.565	0.37
180_R	198_N	0.562	0.37
79_V	181_L	0.562	0.37
76_A	80_R	0.561	0.37
26_P	245_P	0.561	0.37
205_L	247_L	0.560	0.36
155_D	251_D	0.557	0.36
96_N	174_G	0.556	0.36
367_V	370_P	0.556	0.36
117_A	251_D	0.555	0.36
23_L	52_F	0.555	0.36
224_I	228_M	0.554	0.36
8_S	12_H	0.554	0.36
54_H	87_D	0.553	0.36
303_G	311_D	0.552	0.36
268_V	278_Y	0.552	0.36
290_D	342_V	0.552	0.36
109_H	113_H	0.550	0.36
307_A	312_A	0.548	0.35
210_A	214_P	0.547	0.35
171_V	208_A	0.547	0.35
59_Y	220_H	0.546	0.35
358_A	365_L	0.546	0.35
121_V	241_D	0.546	0.35
197_G	218_S	0.545	0.35
110_D	151_A	0.545	0.35
44_Y	219_H	0.545	0.35
67_D	300_T	0.544	0.35
291_D	328_S	0.544	0.35
36_Q	54_H	0.544	0.35
266_S	272_L	0.543	0.35
189_D	253_H	0.542	0.35
35_A	117_A	0.540	0.35
135_I	316_E	0.536	0.34
91_G	156_V	0.536	0.34
205_L	220_H	0.535	0.34
312_A	316_E	0.534	0.34
180_R	184_G	0.532	0.34
158_R	205_L	0.531	0.34
61_L	65_A	0.531	0.34
293_L	297_D	0.531	0.34
15_V	55_S	0.531	0.34
113_H	236_L	0.529	0.34
4_T	183_R	0.529	0.34
294_V	297_D	0.529	0.34
121_V	131_Q	0.529	0.34
305_E	313_G	0.528	0.34
54_H	86_H	0.527	0.33
152_G	155_D	0.527	0.33
174_G	198_N	0.525	0.33
241_D	249_R	0.525	0.33
222_A	226_K	0.523	0.33
337_L	342_V	0.521	0.33
298_H	307_A	0.518	0.33
198_N	212_M	0.518	0.33
93_F	287_G	0.517	0.33
74_Q	131_Q	0.517	0.33
55_S	80_R	0.515	0.32
127_D	304_F	0.515	0.32
299_D	305_E	0.514	0.32
127_D	242_V	0.513	0.32
65_A	149_L	0.512	0.32
89_G	158_R	0.512	0.32
71_I	75_R	0.510	0.32
276_D	317_R	0.510	0.32
303_G	319_V	0.509	0.32
326_E	329_M	0.508	0.32
171_V	216_V	0.507	0.32
43_V	222_A	0.506	0.32
23_L	216_V	0.506	0.32
110_D	158_R	0.505	0.31
211_L	215_T	0.504	0.31
309_A	317_R	0.504	0.31
325_S	328_S	0.503	0.31
109_H	161_Y	0.501	0.31
18_V	77_T	0.501	0.31
233_A	254_D	0.501	0.31
185_L	198_N	0.500	0.31
228_M	234_E	0.500	0.31

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4mz7A	4	0.978	100	0.276	Contact Map
4lrlA	4	0.9438	100	0.317	Contact Map
2hekA	4	0.8778	100	0.322	Contact Map
2q14A	4	0.8655	100	0.361	Contact Map
3bg2A	4	0.6357	100	0.741	Contact Map
2pgsA	4	0.6308	100	0.747	Contact Map
2dqbA	4	0.6528	100	0.748	Contact Map
4x9eA	4	0.6553	100	0.754	Contact Map
3gw7A	2	0.4694	98.7	0.908	Contact Map
2pq7A	2	0.4181	98.6	0.911	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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