Q9HPK4 - HALSA - GREMLIN Contacts

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Q9HPK4 - Uncharacterized protein

UniProt:	Q9HPK4	Sequences: MATELDHHCPNCEAERAFYRTAAMNVHLGLKTKWSCPDCEYGFIRIAADDDISTATA	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	57 (46)
Sequences:	18859 (14579)
Seq/√Len:	2149.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
10_P	37_P	3.811	1.00
21_T	50_D	2.636	1.00
11_N	38_D	2.426	1.00
17_A	46_I	2.233	1.00
8_H	35_S	2.229	1.00
14_A	41_Y	2.006	1.00
35_S	42_G	1.977	1.00
45_R	48_A	1.931	1.00
14_A	43_F	1.674	1.00
17_A	21_T	1.638	1.00
13_E	40_E	1.559	1.00
16_R	19_Y	1.537	1.00
46_I	50_D	1.457	1.00
21_T	24_M	1.363	1.00
35_S	40_E	1.238	1.00
15_E	44_I	1.215	1.00
19_Y	22_A	1.208	1.00
18_F	47_A	1.180	1.00
11_N	24_M	1.175	1.00
10_P	24_M	1.150	1.00
16_R	45_R	1.126	1.00
23_A	27_H	0.962	1.00
37_P	42_G	0.947	1.00
9_C	23_A	0.942	1.00
8_H	13_E	0.938	1.00
19_Y	48_A	0.934	1.00
44_I	48_A	0.926	1.00
36_C	39_C	0.913	1.00
18_F	22_A	0.905	1.00
15_E	19_Y	0.893	1.00
12_C	27_H	0.885	1.00
12_C	23_A	0.800	1.00
14_A	26_V	0.781	1.00
39_C	49_D	0.735	1.00
12_C	20_R	0.731	1.00
7_H	20_R	0.730	1.00
7_H	12_C	0.719	1.00
26_V	44_I	0.716	1.00
45_R	49_D	0.709	1.00
31_K	42_G	0.667	1.00
35_S	38_D	0.657	1.00
22_A	25_N	0.650	1.00
29_G	44_I	0.633	1.00
31_K	34_W	0.632	1.00
36_C	43_F	0.618	1.00
34_W	39_C	0.612	1.00
38_D	49_D	0.608	1.00
5_L	26_V	0.598	1.00
9_C	19_Y	0.586	1.00
31_K	36_C	0.586	1.00
8_H	11_N	0.585	1.00
7_H	34_W	0.578	1.00
43_F	49_D	0.565	1.00
32_T	42_G	0.562	1.00
47_A	50_D	0.554	1.00
17_A	20_R	0.548	1.00
10_P	42_G	0.547	1.00
5_L	15_E	0.546	1.00
7_H	27_H	0.540	1.00
9_C	20_R	0.539	1.00
46_I	49_D	0.538	1.00
19_Y	23_A	0.532	1.00
20_R	23_A	0.530	1.00
24_M	50_D	0.524	1.00
38_D	41_Y	0.513	1.00
25_N	30_L	0.507	1.00
8_H	37_P	0.507	1.00
15_E	18_F	0.500	1.00
5_L	31_K	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3w5kB	1	0.9474	98.7	0.327	Contact Map
2i13A	1	0.9649	98.7	0.328	Contact Map
1ej6B	7	0.7719	98.7	0.332	Contact Map
1x6eA	1	0.9825	98.7	0.333	Contact Map
2lv2A	1	0.9825	98.7	0.338	Contact Map
3uk3C	1	0.8772	98.7	0.339	Contact Map
2lceA	1	0.9825	98.7	0.342	Contact Map
2n25A	1	0.0877	98.7	0.347	Contact Map
2wbtA	2	0.9474	98.6	0.349	Contact Map
2gqjA	1	0.9825	98.6	0.351	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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