Q9HNV2 - HALSA - GREMLIN Contacts

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Q9HNV2 - Cell division protein

UniProt:	Q9HNV2	Sequences: MGLEHIPEENRVLIGQSRVKGHGVGADNELGAEIAEEDIDEVQGAIDSIPVHEVDAFLVISGLGGGTGSGGSPVIAKHLKRIYTEPVYGIGVLPGSDEGGIYTLNAARSFQTFVREVDNLLVFDNDAWRKSGESVQGGYDEINEEIVTRFGILFGAGEVEQGGDVAESVVDSSEIINTLAGGGVSTIGYASETVDNNSGGAGSGLLSRFTGDDDELEDTASTTNRITSLVRKAALGRLTLPCEIEGSERALLVTAGPPKYLNRKGIERGRKWLEDQTGSMEVRGGDYPVSNSDQVASVVLLSGVNDVPRIKELQEVAIEAQDNIDEIREESEDNLEELVEDDEDELEPLF	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
Length:	350 (279)
Sequences:	2731 (1326)
Seq/√Len:	79.4

ECOLI - FTSZ
BACSU - FTSZ
HALSA - Q9HNV2 FTSZ1 FTSZ2 Q9HHS0 Q9HSE9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
32_A	112_T	4.776	1.00
39_I	77_K	4.274	1.00
39_I	78_H	4.162	1.00
69_S	109_S	4.099	1.00
112_T	115_R	3.916	1.00
191_S	294_Q	3.839	1.00
96_S	126_D	3.828	1.00
38_D	41_E	3.243	1.00
77_K	116_E	3.194	1.00
59_V	72_S	3.061	1.00
196_N	228_S	3.058	1.00
197_N	265_G	3.018	1.00
44_G	47_D	2.995	1.00
89_G	117_V	2.974	1.00
117_V	120_L	2.973	1.00
193_T	294_Q	2.808	1.00
77_K	81_R	2.700	1.00
252_L	270_R	2.656	1.00
93_L	103_T	2.623	1.00
63_L	110_F	2.615	1.00
141_E	144_E	2.576	1.00
144_E	147_V	2.426	1.00
176_I	180_A	2.350	1.00
179_L	302_S	2.315	1.00
43_Q	81_R	2.280	1.00
39_I	43_Q	2.189	1.00
259_K	290_S	2.164	1.00
100_G	104_L	2.156	1.00
32_A	36_E	2.102	1.00
104_L	108_R	2.093	1.00
93_L	107_A	2.077	1.00
33_E	36_E	2.059	1.00
256_G	261_L	2.043	1.00
89_G	120_L	2.041	1.00
43_Q	82_I	2.006	1.00
43_Q	78_H	1.936	1.00
228_S	231_R	1.921	1.00
49_I	57_F	1.912	1.00
227_T	268_R	1.901	1.00
111_Q	115_R	1.873	1.00
192_E	228_S	1.839	1.00
46_I	82_I	1.811	1.00
156_A	170_V	1.775	1.00
147_V	151_G	1.773	1.00
41_E	44_G	1.746	1.00
12_V	45_A	1.723	1.00
234_A	299_V	1.690	1.00
46_I	57_F	1.678	1.00
230_V	273_L	1.664	1.00
191_S	296_A	1.646	1.00
271_K	275_D	1.637	1.00
108_R	112_T	1.607	1.00
178_T	249_R	1.603	1.00
132_G	264_K	1.602	1.00
29_E	33_E	1.590	1.00
40_D	43_Q	1.581	1.00
227_T	231_R	1.577	1.00
35_A	71_G	1.569	1.00
78_H	82_I	1.553	0.99
266_I	284_G	1.550	0.99
166_A	287_Y	1.549	0.99
134_S	137_G	1.541	0.99
257_P	260_Y	1.538	0.99
89_G	113_F	1.537	0.99
36_E	77_K	1.537	0.99
260_Y	293_D	1.518	0.99
119_N	179_L	1.516	0.99
29_E	32_A	1.480	0.99
26_A	177_N	1.472	0.99
167_E	286_D	1.464	0.99
61_S	91_G	1.440	0.99
267_E	271_K	1.422	0.99
20_K	55_D	1.420	0.99
168_S	284_G	1.398	0.99
194_V	197_N	1.373	0.99
261_L	295_V	1.370	0.99
133_E	138_G	1.359	0.99
42_V	78_H	1.339	0.98
250_A	277_T	1.338	0.98
26_A	283_R	1.335	0.98
186_T	242_C	1.331	0.98
107_A	111_Q	1.305	0.98
90_I	150_F	1.294	0.98
137_G	140_D	1.285	0.98
135_V	286_D	1.278	0.98
149_R	187_I	1.266	0.98
236_G	240_L	1.260	0.98
101_I	281_E	1.234	0.97
136_Q	288_P	1.234	0.97
249_R	280_M	1.233	0.97
49_I	84_T	1.215	0.97
140_D	143_N	1.210	0.97
249_R	278_G	1.205	0.97
277_T	280_M	1.197	0.97
119_N	182_G	1.197	0.97
27_D	177_N	1.184	0.97
112_T	116_E	1.178	0.96
14_I	71_G	1.156	0.96
253_V	287_Y	1.147	0.96
16_Q	41_E	1.134	0.96
231_R	235_L	1.126	0.95
198_S	230_V	1.107	0.95
57_F	84_T	1.104	0.95
193_T	293_D	1.100	0.95
127_A	130_K	1.099	0.95
229_L	297_S	1.099	0.95
13_L	16_Q	1.088	0.95
269_G	295_V	1.083	0.94
108_R	111_Q	1.076	0.94
99_G	282_V	1.070	0.94
129_R	267_E	1.069	0.94
245_E	276_Q	1.069	0.94
151_G	155_G	1.061	0.94
314_Q	318_I	1.047	0.93
12_V	42_V	1.042	0.93
18_R	33_E	1.039	0.93
128_W	142_I	1.024	0.93
260_Y	292_S	1.016	0.92
57_F	75_I	1.010	0.92
43_Q	47_D	1.007	0.92
101_I	105_N	1.006	0.92
96_S	271_K	0.999	0.92
166_A	253_V	0.998	0.92
255_A	287_Y	0.995	0.91
55_D	129_R	0.990	0.91
10_N	45_A	0.988	0.91
133_E	264_K	0.987	0.91
178_T	283_R	0.984	0.91
135_V	139_Y	0.982	0.91
24_V	30_L	0.979	0.91
227_T	272_W	0.970	0.90
271_K	274_E	0.962	0.90
42_V	46_I	0.958	0.90
103_T	107_A	0.956	0.90
40_D	44_G	0.953	0.90
132_G	262_N	0.953	0.90
256_G	266_I	0.944	0.89
261_L	266_I	0.943	0.89
102_Y	283_R	0.942	0.89
249_R	303_G	0.937	0.89
93_L	124_D	0.932	0.88
46_I	49_I	0.930	0.88
134_S	262_N	0.923	0.88
289_V	296_A	0.920	0.88
272_W	275_D	0.916	0.88
110_F	122_V	0.910	0.87
128_W	263_R	0.910	0.87
248_E	278_G	0.904	0.87
318_I	321_Q	0.901	0.87
196_N	268_R	0.900	0.87
128_W	143_N	0.900	0.87
228_S	232_K	0.894	0.86
252_L	297_S	0.894	0.86
310_I	313_L	0.886	0.86
270_R	284_G	0.881	0.86
143_N	270_R	0.880	0.85
27_D	33_E	0.880	0.85
5_H	271_K	0.879	0.85
126_D	129_R	0.876	0.85
256_G	295_V	0.872	0.85
313_L	319_E	0.867	0.85
126_D	130_K	0.861	0.84
74_V	78_H	0.860	0.84
7_P	10_N	0.857	0.84
316_V	321_Q	0.855	0.84
313_L	317_A	0.850	0.83
155_G	158_E	0.849	0.83
188_G	233_A	0.848	0.83
104_L	107_A	0.846	0.83
310_I	314_Q	0.845	0.83
149_R	240_L	0.842	0.83
236_G	304_V	0.837	0.82
111_Q	114_V	0.825	0.82
182_G	189_Y	0.817	0.81
198_S	295_V	0.812	0.81
63_L	91_G	0.810	0.80
127_A	236_G	0.806	0.80
35_A	42_V	0.805	0.80
114_V	184_V	0.805	0.80
272_W	276_Q	0.804	0.80
184_V	252_L	0.798	0.79
269_G	297_S	0.798	0.79
134_S	147_V	0.790	0.79
310_I	315_E	0.785	0.78
74_V	77_K	0.781	0.78
131_S	264_K	0.776	0.77
231_R	272_W	0.775	0.77
160_E	167_E	0.768	0.77
178_T	280_M	0.766	0.77
249_R	281_E	0.765	0.76
181_G	240_L	0.760	0.76
93_L	240_L	0.760	0.76
59_V	76_A	0.756	0.76
16_Q	38_D	0.752	0.75
229_L	233_A	0.752	0.75
93_L	110_F	0.751	0.75
314_Q	317_A	0.751	0.75
267_E	274_E	0.749	0.75
177_N	283_R	0.747	0.75
309_R	312_E	0.746	0.75
61_S	72_S	0.744	0.74
315_E	318_I	0.743	0.74
56_A	268_R	0.741	0.74
101_I	104_L	0.736	0.74
35_A	74_V	0.735	0.74
313_L	316_V	0.734	0.73
30_L	33_E	0.733	0.73
6_I	11_R	0.730	0.73
143_N	284_G	0.730	0.73
257_P	262_N	0.728	0.73
56_A	87_V	0.726	0.73
152_I	187_I	0.724	0.72
153_L	158_E	0.723	0.72
131_S	268_R	0.722	0.72
18_R	30_L	0.721	0.72
136_Q	259_K	0.716	0.72
151_G	289_V	0.716	0.72
59_V	75_I	0.715	0.71
314_Q	321_Q	0.714	0.71
23_G	66_G	0.710	0.71
59_V	89_G	0.709	0.71
80_K	181_G	0.706	0.70
263_R	286_D	0.704	0.70
250_A	301_L	0.703	0.70
93_L	300_L	0.701	0.70
56_A	121_L	0.698	0.70
47_D	82_I	0.696	0.69
11_R	304_V	0.695	0.69
81_R	177_N	0.695	0.69
130_K	134_S	0.695	0.69
39_I	42_V	0.695	0.69
156_A	300_L	0.693	0.69
295_V	307_V	0.693	0.69
104_L	279_S	0.690	0.69
126_D	267_E	0.690	0.69
192_E	198_S	0.690	0.69
58_L	185_S	0.689	0.69
194_V	198_S	0.686	0.68
159_V	287_Y	0.684	0.68
25_G	66_G	0.684	0.68
182_G	249_R	0.682	0.68
312_E	321_Q	0.679	0.68
313_L	318_I	0.674	0.67
84_T	186_T	0.671	0.67
190_A	229_L	0.670	0.66
151_G	189_Y	0.670	0.66
312_E	317_A	0.667	0.66
312_E	316_V	0.665	0.66
315_E	320_A	0.664	0.66
256_G	288_P	0.662	0.66
308_P	311_K	0.660	0.65
152_I	170_V	0.659	0.65
316_V	320_A	0.659	0.65
97_D	284_G	0.657	0.65
309_R	313_L	0.657	0.65
197_N	262_N	0.654	0.65
157_G	175_I	0.653	0.64
136_Q	260_Y	0.652	0.64
4_E	11_R	0.649	0.64
23_G	68_G	0.647	0.64
313_L	321_Q	0.645	0.64
78_H	81_R	0.644	0.63
89_G	110_F	0.641	0.63
105_N	108_R	0.639	0.63
261_L	284_G	0.638	0.63
311_K	319_E	0.636	0.62
130_K	227_T	0.635	0.62
26_A	105_N	0.634	0.62
105_N	304_V	0.633	0.62
184_V	305_N	0.632	0.62
14_I	46_I	0.631	0.62
110_F	120_L	0.629	0.62
158_E	252_L	0.628	0.61
11_R	44_G	0.626	0.61
314_Q	319_E	0.625	0.61
103_T	126_D	0.624	0.61
17_S	37_E	0.624	0.61
119_N	227_T	0.623	0.61
152_I	298_V	0.623	0.61
314_Q	320_A	0.621	0.61
56_A	179_L	0.621	0.61
189_Y	240_L	0.620	0.61
167_E	288_P	0.618	0.60
248_E	303_G	0.618	0.60
311_K	315_E	0.613	0.60
158_E	260_Y	0.611	0.59
13_L	63_L	0.611	0.59
35_A	150_F	0.610	0.59
256_G	264_K	0.609	0.59
40_D	45_A	0.609	0.59
315_E	319_E	0.609	0.59
177_N	281_E	0.606	0.59
60_I	146_I	0.606	0.59
130_K	136_Q	0.606	0.59
143_N	266_I	0.605	0.59
14_I	136_Q	0.604	0.59
4_E	111_Q	0.604	0.59
13_L	37_E	0.603	0.58
254_T	264_K	0.603	0.58
90_I	146_I	0.601	0.58
260_Y	282_V	0.599	0.58
89_G	153_L	0.599	0.58
124_D	145_E	0.599	0.58
123_F	177_N	0.598	0.58
21_G	66_G	0.597	0.58
5_H	160_E	0.596	0.58
264_K	267_E	0.592	0.57
122_V	130_K	0.592	0.57
243_E	250_A	0.592	0.57
192_E	297_S	0.592	0.57
160_E	287_Y	0.591	0.57
14_I	38_D	0.590	0.57
119_N	133_E	0.589	0.57
91_G	122_V	0.588	0.56
153_L	175_I	0.588	0.56
317_A	320_A	0.586	0.56
300_L	304_V	0.585	0.56
129_R	264_K	0.585	0.56
243_E	276_Q	0.583	0.56
170_V	283_R	0.582	0.56
20_K	140_D	0.581	0.56
262_N	288_P	0.581	0.56
255_A	302_S	0.580	0.55
248_E	305_N	0.580	0.55
149_R	241_P	0.579	0.55
230_V	254_T	0.578	0.55
41_E	47_D	0.578	0.55
263_R	281_E	0.578	0.55
179_L	185_S	0.577	0.55
190_A	233_A	0.576	0.55
60_I	132_G	0.575	0.55
119_N	185_S	0.574	0.55
6_I	243_E	0.574	0.55
280_M	296_A	0.574	0.55
227_T	275_D	0.573	0.55
26_A	34_I	0.572	0.54
277_T	282_V	0.572	0.54
308_P	313_L	0.571	0.54
49_I	55_D	0.569	0.54
80_K	232_K	0.569	0.54
309_R	320_A	0.568	0.54
128_W	170_V	0.567	0.54
138_G	263_R	0.565	0.54
45_A	49_I	0.565	0.54
97_D	139_Y	0.564	0.53
309_R	318_I	0.562	0.53
257_P	292_S	0.562	0.53
173_S	247_S	0.559	0.53
139_Y	168_S	0.558	0.53
129_R	152_I	0.557	0.53
312_E	315_E	0.553	0.52
309_R	319_E	0.553	0.52
310_I	317_A	0.552	0.52
307_V	310_I	0.552	0.52
60_I	142_I	0.551	0.52
317_A	321_Q	0.551	0.52
298_V	304_V	0.551	0.52
311_K	318_I	0.550	0.52
182_G	302_S	0.550	0.52
182_G	280_M	0.549	0.51
134_S	306_D	0.548	0.51
55_D	173_S	0.548	0.51
193_T	309_R	0.546	0.51
6_I	122_V	0.545	0.51
12_V	38_D	0.545	0.51
27_D	108_R	0.545	0.51
36_E	39_I	0.544	0.51
315_E	321_Q	0.543	0.51
26_A	150_F	0.541	0.50
154_F	157_G	0.540	0.50
121_L	147_V	0.538	0.50
123_F	142_I	0.538	0.50
91_G	113_F	0.536	0.50
28_N	35_A	0.535	0.50
101_I	279_S	0.534	0.50
135_V	182_G	0.534	0.50
38_D	184_V	0.533	0.49
123_F	311_K	0.533	0.49
133_E	304_V	0.533	0.49
168_S	286_D	0.531	0.49
39_I	81_R	0.531	0.49
38_D	75_I	0.531	0.49
131_S	195_D	0.530	0.49
87_V	302_S	0.528	0.49
57_F	79_L	0.527	0.49
231_R	251_L	0.526	0.49
259_K	292_S	0.526	0.49
188_G	275_D	0.525	0.48
80_K	267_E	0.525	0.48
117_V	253_V	0.524	0.48
313_L	320_A	0.523	0.48
137_G	264_K	0.522	0.48
124_D	241_P	0.520	0.48
72_S	75_I	0.519	0.48
197_N	261_L	0.519	0.48
38_D	259_K	0.517	0.47
100_G	278_G	0.514	0.47
152_I	160_E	0.513	0.47
234_A	272_W	0.512	0.47
10_N	251_L	0.512	0.47
58_L	175_I	0.510	0.46
232_K	235_L	0.509	0.46
254_T	269_G	0.508	0.46
56_A	175_I	0.507	0.46
8_E	74_V	0.507	0.46
151_G	159_V	0.506	0.46
135_V	266_I	0.505	0.46
114_V	305_N	0.505	0.46
308_P	315_E	0.505	0.46
149_R	189_Y	0.504	0.46
86_P	172_S	0.504	0.46
310_I	321_Q	0.502	0.45
159_V	300_L	0.502	0.45
123_F	129_R	0.501	0.45
29_E	108_R	0.500	0.45
32_A	235_L	0.500	0.45
121_L	143_N	0.500	0.45
245_E	300_L	0.500	0.45

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4b46A	1	0.8171	100	0.354	Contact Map
2r751	1	0.8057	100	0.387	Contact Map
2vapA	1	0.7914	100	0.392	Contact Map
4b45A	1	0.8314	100	0.396	Contact Map
2vawA	1	0.7543	100	0.397	Contact Map
4m8iA	1	0.7686	100	0.399	Contact Map
4ei7A	1	0.84	100	0.399	Contact Map
1ofuA	1	0.7571	100	0.4	Contact Map
1rq2A	2	0.74	100	0.401	Contact Map
2vxyA	2	0.7543	100	0.402	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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