GREMLIN Database
Q9HNT7 - Subtilisin homolog
UniProt: Q9HNT7 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 585 (467)
Sequences: 4016 (3218)
Seq/√Len: 148.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
289_S425_A4.9791.00
320_G355_A4.7091.00
151_I197_S4.5791.00
108_G232_A4.3331.00
319_S368_S4.3201.00
431_L472_T3.8991.00
310_G317_S3.7041.00
431_L474_P3.6731.00
154_V225_V3.4611.00
378_G416_G3.4521.00
192_H423_M3.1251.00
285_V316_I3.1131.00
152_A246_A3.0801.00
145_T217_D2.9871.00
456_A473_T2.9491.00
537_D540_V2.8681.00
169_T172_S2.8061.00
320_G357_Y2.7601.00
357_Y372_G2.7191.00
194_A222_D2.6701.00
355_A372_G2.6441.00
289_S424_A2.6011.00
55_I59_Q2.5461.00
320_G372_G2.4491.00
168_D172_S2.4411.00
54_V58_A2.4261.00
195_G376_S2.3861.00
544_D547_L2.3621.00
354_P368_S2.3431.00
147_E217_D2.3421.00
55_I58_A2.3331.00
378_G418_L2.3091.00
537_D542_M2.2591.00
121_I289_S2.1901.00
196_I427_H2.1891.00
242_I284_A2.1721.00
367_V472_T2.0881.00
169_T173_G2.0481.00
425_A429_T2.0471.00
185_A188_S2.0451.00
285_V432_A2.0341.00
234_I253_L1.9991.00
167_V170_S1.9931.00
169_T175_F1.9801.00
153_I197_S1.9781.00
225_V237_A1.9701.00
473_T476_T1.9671.00
221_L246_A1.9631.00
199_A377_S1.9441.00
225_V253_L1.9261.00
321_T368_S1.9211.00
540_V544_D1.9001.00
436_A447_I1.8981.00
428_V472_T1.8951.00
288_E315_W1.8701.00
238_A279_A1.8681.00
167_V172_S1.8521.00
226_F231_S1.8311.00
443_T446_Q1.8231.00
195_G377_S1.8231.00
250_N425_A1.8221.00
41_Q101_V1.8191.00
168_D171_R1.7951.00
542_M547_L1.7941.00
161_S184_S1.7551.00
539_V542_M1.7551.00
250_N429_T1.7461.00
139_I478_D1.7411.00
452_K469_A1.7331.00
196_I423_M1.7171.00
539_V544_D1.7111.00
537_D544_D1.6821.00
175_F240_T1.6621.00
172_S175_F1.6561.00
442_A450_H1.6531.00
214_V430_G1.6481.00
102_P105_L1.6391.00
58_A61_V1.6281.00
367_V451_I1.6141.00
439_N442_A1.5951.00
192_H196_I1.5891.00
158_I220_L1.5781.00
168_D173_G1.5761.00
542_M546_E1.5751.00
174_N177_L1.5661.00
54_V57_E1.5551.00
543_A546_E1.5511.00
322_G372_G1.5441.00
533_D537_D1.5431.00
148_G219_T1.5331.00
544_D549_Y1.5221.00
93_I105_L1.5171.00
438_Q478_D1.5151.00
41_Q103_D1.5071.00
370_P374_V1.5021.00
198_A218_A1.4991.00
170_S173_G1.4971.00
154_V237_A1.4741.00
153_I194_A1.4651.00
454_T477_Y1.4541.00
544_D548_L1.4421.00
448_I452_K1.4411.00
354_P466_Y1.4321.00
167_V171_R1.4291.00
59_Q62_G1.4291.00
287_V429_T1.4131.00
368_S424_A1.4091.00
357_Y419_H1.3941.00
238_A251_L1.3941.00
19_L22_T1.3941.00
375_G417_T1.3911.00
354_P358_T1.3901.00
158_I222_D1.3851.00
150_T219_T1.3801.00
540_V547_L1.3761.00
58_A62_G1.3751.00
89_D92_T1.3741.00
35_Q108_G1.3741.00
276_G315_W1.3651.00
192_H376_S1.3531.00
535_N540_V1.3531.00
56_D60_S1.3511.00
542_M545_V1.3471.00
16_I19_L1.3461.00
373_N419_H1.3461.00
159_D184_S1.3451.00
369_A428_V1.3421.00
367_V428_V1.3381.00
39_D103_D1.3381.00
287_V428_V1.3351.00
431_L451_I1.3301.00
235_L275_I1.3201.00
54_V59_Q1.3151.00
543_A547_L1.3001.00
55_I60_S1.2951.00
59_Q63_F1.2831.00
174_N179_D1.2811.00
168_D174_N1.2811.00
435_V447_I1.2791.00
545_V548_L1.2781.00
294_D359_N1.2781.00
238_A276_G1.2651.00
277_E314_G1.2581.00
449_A453_H1.2531.00
144_V437_A1.2471.00
167_V175_F1.2371.00
223_M237_A1.2321.00
248_A285_V1.2311.00
135_N473_T1.2281.00
163_P414_M1.2281.00
152_A241_A1.2251.00
376_S423_M1.2221.00
214_V431_L1.2141.00
193_C250_N1.2131.00
149_T436_A1.2111.00
270_K273_R1.2031.00
287_V425_A1.1961.00
540_V548_L1.1921.00
360_Y466_Y1.1921.00
318_V367_V1.1881.00
492_R502_A1.1871.00
151_I433_A1.1861.00
56_D59_Q1.1841.00
316_I428_V1.1721.00
496_H499_I1.1711.00
58_A63_F1.1711.00
523_A527_D1.1661.00
165_L194_A1.1651.00
165_L377_S1.1641.00
542_M549_Y1.1621.00
168_D175_F1.1621.00
95_G98_P1.1611.00
541_D544_D1.1601.00
167_V174_N1.1571.00
198_A220_L1.1561.00
170_S175_F1.1531.00
535_N542_M1.1521.00
528_D532_E1.1521.00
352_H471_D1.1501.00
96_V102_P1.1481.00
156_S185_A1.1320.99
428_V451_I1.1310.99
125_H128_Q1.1300.99
153_I193_C1.1270.99
215_A433_A1.1260.99
242_I278_L1.1230.99
169_T174_N1.1160.99
197_S430_G1.1140.99
540_V546_E1.1060.99
280_V285_V1.1040.99
527_D530_V1.1020.99
321_T471_D1.0990.99
355_A419_H1.0980.99
170_S219_T1.0920.99
24_Q28_A1.0880.99
537_D545_V1.0840.99
152_A221_L1.0820.99
167_V177_L1.0820.99
94_T97_S1.0810.99
146_G215_A1.0750.99
241_A249_V1.0720.99
521_Y527_D1.0620.99
435_V477_Y1.0600.99
285_V444_T1.0590.99
242_I279_A1.0480.99
22_T26_V1.0480.99
523_A532_E1.0380.99
515_F523_A1.0320.99
156_S187_S1.0270.99
538_G544_D1.0260.99
15_A18_G1.0260.99
143_D146_G1.0250.99
192_H418_L1.0240.99
158_I165_L1.0240.99
316_I448_I1.0200.99
223_M240_T1.0190.99
226_F233_S1.0180.99
295_T360_Y1.0160.99
534_V537_D1.0150.99
540_V549_Y1.0130.99
247_D436_A1.0100.99
446_Q483_E1.0100.99
538_G542_M1.0060.99
169_T176_S1.0030.99
149_T433_A1.0020.99
537_D547_L1.0010.99
273_R312_H1.0010.99
234_I251_L1.0000.99
240_T244_A0.9990.99
223_M244_A0.9960.99
60_S64_A0.9930.99
108_G111_S0.9910.99
542_M548_L0.9860.99
171_R174_N0.9860.99
527_D532_E0.9830.99
151_I218_A0.9820.99
167_V173_G0.9800.99
186_D228_F0.9750.98
171_R175_F0.9720.98
182_G185_A0.9700.98
537_D548_L0.9690.98
124_P127_L0.9680.98
214_V427_H0.9680.98
240_T243_R0.9670.98
312_H315_W0.9590.98
418_L423_M0.9590.98
224_K227_P0.9580.98
520_Y523_A0.9570.98
14_A17_G0.9570.98
57_E60_S0.9540.98
455_A470_G0.9530.98
227_P230_S0.9500.98
139_I142_N0.9490.98
248_A429_T0.9440.98
26_V29_E0.9430.98
538_G541_D0.9420.98
540_V545_V0.9390.98
442_A446_Q0.9390.98
546_E549_Y0.9380.98
434_V474_P0.9360.98
492_R496_H0.9360.98
533_D540_V0.9360.98
540_V543_A0.9340.98
543_A548_L0.9290.98
484_D511_G0.9240.98
129_W133_E0.9240.98
127_L132_A0.9220.98
528_D531_Y0.9150.98
238_A249_V0.9130.98
160_A184_S0.9100.98
64_A68_G0.9100.98
538_G549_Y0.9100.98
150_T221_L0.9090.98
54_V63_F0.9050.98
151_I198_A0.9040.98
56_D61_V0.9030.98
194_A220_L0.9010.97
138_A142_N0.8970.97
515_F518_T0.8960.97
80_F239_E0.8960.97
248_A447_I0.8940.97
276_G288_E0.8940.97
251_L288_E0.8930.97
16_I21_A0.8870.97
154_V223_M0.8860.97
34_D107_D0.8860.97
492_R499_I0.8850.97
278_L284_A0.8820.97
127_L130_S0.8780.97
538_G543_A0.8770.97
277_E313_P0.8760.97
137_A141_D0.8720.97
227_P233_S0.8720.97
135_N138_A0.8710.97
165_L195_G0.8690.97
169_T177_L0.8670.97
55_I61_V0.8670.97
54_V62_G0.8640.97
106_P271_A0.8620.97
283_D444_T0.8610.97
152_A249_V0.8600.97
175_F223_M0.8580.97
354_P360_Y0.8570.97
318_V428_V0.8560.97
295_T298_D0.8550.97
19_L23_T0.8530.96
320_G419_H0.8490.96
275_I279_A0.8490.96
249_V279_A0.8490.96
238_A275_I0.8470.96
77_G85_E0.8440.96
533_D538_G0.8440.96
77_G83_E0.8440.96
538_G547_L0.8430.96
422_S426_P0.8410.96
369_A472_T0.8400.96
125_H129_W0.8390.96
270_K274_R0.8380.96
370_P427_H0.8380.96
456_A471_D0.8350.96
464_V467_F0.8340.96
22_T25_P0.8320.96
136_V140_H0.8320.96
325_S471_D0.8300.96
111_S234_I0.8280.96
142_N475_D0.8240.96
92_T95_G0.8230.96
486_Y490_T0.8160.95
235_L271_A0.8160.95
293_A359_N0.8100.95
153_I222_D0.8100.95
83_E86_L0.8100.95
39_D81_A0.8090.95
26_V30_G0.8080.95
52_V55_I0.8070.95
439_N450_H0.8050.95
134_Q214_V0.8040.95
126_P214_V0.8040.95
167_V176_S0.8040.95
435_V474_P0.8030.95
62_G65_S0.8030.95
475_D479_N0.8030.95
432_A447_I0.8020.95
77_G84_F0.7990.95
188_S418_L0.7990.95
122_P125_H0.7980.95
233_S236_N0.7940.95
122_P128_Q0.7920.95
187_S231_S0.7900.95
482_L513_L0.7890.95
37_L40_T0.7890.95
154_V238_A0.7850.94
49_R52_V0.7840.94
445_R485_R0.7830.94
168_D177_L0.7820.94
138_A141_D0.7810.94
515_F519_T0.7810.94
150_T245_G0.7780.94
535_N538_G0.7780.94
122_P126_P0.7780.94
346_G350_P0.7770.94
39_D104_E0.7770.94
307_T359_N0.7760.94
255_P258_P0.7760.94
61_V65_S0.7740.94
170_S174_N0.7710.94
188_S378_G0.7710.94
279_A286_L0.7710.94
454_T480_G0.7700.94
17_G20_S0.7700.94
313_P363_D0.7690.94
131_K134_Q0.7690.94
523_A530_V0.7680.94
290_A317_S0.7680.94
55_I63_F0.7680.94
226_F253_L0.7670.94
241_A245_G0.7660.94
475_D478_D0.7660.94
246_A284_A0.7650.94
199_A211_A0.7650.94
140_H143_D0.7640.94
215_A218_A0.7630.94
60_S63_F0.7630.94
539_V547_L0.7630.94
19_L27_T0.7620.94
196_I376_S0.7580.93
80_F83_E0.7580.93
196_I418_L0.7560.93
78_L82_P0.7550.93
285_V447_I0.7550.93
431_L434_V0.7530.93
280_V444_T0.7530.93
111_S226_F0.7530.93
164_D199_A0.7490.93
317_S448_I0.7490.93
138_A475_D0.7480.93
436_A442_A0.7470.93
48_W51_A0.7440.93
155_D252_S0.7420.93
164_D377_S0.7420.93
241_A246_A0.7420.93
545_V549_Y0.7410.93
69_S72_A0.7410.93
79_D239_E0.7390.92
85_E232_A0.7390.92
58_A64_A0.7360.92
277_E281_D0.7350.92
59_Q66_V0.7340.92
225_V251_L0.7310.92
123_Q126_P0.7290.92
224_K228_F0.7280.92
39_D106_P0.7280.92
533_D542_M0.7260.92
35_Q87_S0.7260.92
150_T246_A0.7240.92
93_I97_S0.7230.92
137_A140_H0.7210.92
23_T27_T0.7210.92
54_V61_V0.7210.92
155_D189_H0.7190.91
17_G23_T0.7190.91
435_V442_A0.7170.91
436_A440_P0.7170.91
492_R497_I0.7150.91
423_M426_P0.7140.91
253_L288_E0.7130.91
66_V70_E0.7120.91
193_C425_A0.7120.91
317_S366_D0.7110.91
498_D501_R0.7100.91
148_G215_A0.7070.91
366_D452_K0.7060.91
223_M241_A0.7050.91
423_M427_H0.7050.91
486_Y491_F0.7040.91
140_H145_T0.7030.90
245_G284_A0.7010.90
25_P28_A0.7000.90
79_D83_E0.7000.90
171_R176_S0.6990.90
73_L76_Y0.6970.90
144_V438_Q0.6940.90
24_Q27_T0.6940.90
373_N417_T0.6930.90
288_E310_G0.6930.90
163_P166_T0.6920.90
51_A55_I0.6900.90
21_A25_P0.6900.90
171_R177_L0.6890.90
537_D546_E0.6850.89
171_R221_L0.6830.89
39_D42_S0.6820.89
432_A435_V0.6810.89
64_A69_S0.6790.89
292_N422_S0.6790.89
53_D58_A0.6780.89
321_T354_P0.6770.89
234_I237_A0.6770.89
55_I62_G0.6770.89
80_F242_I0.6760.89
346_G349_S0.6760.89
521_Y531_Y0.6760.89
316_I432_A0.6750.89
409_Y412_S0.6740.89
525_P528_D0.6680.88
75_G85_E0.6680.88
46_D52_V0.6670.88
47_A51_A0.6660.88
535_N549_Y0.6650.88
158_I194_A0.6640.88
252_S426_P0.6640.88
51_A54_V0.6620.88
529_G532_E0.6620.88
193_C196_I0.6610.88
27_T31_V0.6600.87
123_Q128_Q0.6580.87
527_D531_Y0.6580.87
54_V60_S0.6580.87
323_P374_V0.6580.87
538_G545_V0.6570.87
14_A19_L0.6570.87
149_T437_A0.6570.87
457_H464_V0.6540.87
62_G66_V0.6540.87
173_G177_L0.6520.87
255_P259_V0.6520.87
374_V427_H0.6500.87
464_V469_A0.6490.87
251_L276_G0.6490.87
182_G224_K0.6480.87
135_N475_D0.6470.86
280_V448_I0.6460.86
516_D523_A0.6440.86
359_N365_V0.6420.86
170_S176_S0.6410.86
165_L169_T0.6410.86
537_D543_A0.6410.86
362_P365_V0.6400.86
96_V99_A0.6400.86
483_E514_E0.6390.86
163_P413_G0.6390.86
18_G26_V0.6380.86
482_L523_A0.6380.86
39_D278_L0.6370.86
146_G434_V0.6350.85
237_A241_A0.6330.85
308_G311_S0.6300.85
59_Q64_A0.6290.85
62_G70_E0.6290.85
172_S222_D0.6270.85
142_N479_N0.6270.85
242_I282_N0.6270.85
125_H130_S0.6260.85
287_V316_I0.6250.85
536_G542_M0.6250.85
130_S427_H0.6240.84
18_G24_Q0.6240.84
49_R53_D0.6230.84
174_N224_K0.6220.84
45_S48_W0.6220.84
168_D200_T0.6210.84
536_G543_A0.6190.84
533_D539_V0.6190.84
287_V432_A0.6190.84
130_S134_Q0.6190.84
511_G515_F0.6160.84
168_D176_S0.6150.84
189_H422_S0.6150.84
192_H374_V0.6140.83
372_G419_H0.6140.83
21_A26_V0.6130.83
120_D156_S0.6120.83
239_E278_L0.6120.83
509_F515_F0.6060.83
61_V64_A0.6060.83
57_E61_V0.6050.83
197_S218_A0.6050.83
66_V71_Q0.6050.83
533_D544_D0.6040.82
139_I475_D0.6020.82
123_Q130_S0.6020.82
280_V286_L0.6000.82
347_T350_P0.6000.82
536_G539_V0.5980.82
136_V139_I0.5980.82
14_A18_G0.5970.82
253_L272_Y0.5970.82
539_V543_A0.5960.82
254_G289_S0.5950.82
123_Q127_L0.5950.82
543_A549_Y0.5940.81
241_A279_A0.5940.81
204_A208_G0.5940.81
192_H427_H0.5920.81
513_L516_D0.5910.81
53_D57_E0.5910.81
154_V251_L0.5910.81
128_Q131_K0.5900.81
156_S254_G0.5880.81
256_S293_A0.5880.81
450_H480_G0.5870.81
67_S70_E0.5870.81
61_V68_G0.5870.81
221_L244_A0.5860.81
273_R276_G0.5860.81
140_H434_V0.5860.81
88_I92_T0.5850.80
109_A269_V0.5850.80
111_S255_P0.5840.80
23_T26_V0.5830.80
255_P309_K0.5820.80
58_A67_S0.5810.80
20_S23_T0.5790.80
164_D211_A0.5790.80
350_P353_T0.5790.80
536_G540_V0.5770.80
191_T377_S0.5770.80
149_T152_A0.5770.80
62_G67_S0.5750.79
296_N307_T0.5750.79
149_T248_A0.5750.79
533_D547_L0.5740.79
350_P460_S0.5730.79
280_V445_R0.5720.79
52_V58_A0.5700.79
17_G26_V0.5690.79
15_A20_S0.5690.79
482_L485_R0.5680.78
535_N546_E0.5670.78
56_D63_F0.5660.78
241_A286_L0.5650.78
145_T437_A0.5650.78
327_V344_D0.5640.78
65_S69_S0.5630.78
46_D53_D0.5630.78
20_S25_P0.5630.78
146_G218_A0.5620.78
518_T521_Y0.5610.78
503_V523_A0.5610.78
35_Q38_V0.5610.78
131_K136_V0.5600.78
189_H292_N0.5600.78
40_T84_F0.5590.77
446_Q514_E0.5590.77
140_H144_V0.5570.77
290_A424_A0.5560.77
535_N547_L0.5560.77
369_A424_A0.5560.77
497_I500_Q0.5540.77
201_G212_V0.5540.77
20_S26_V0.5540.77
128_Q133_E0.5530.77
367_V431_L0.5530.77
43_A46_D0.5510.76
250_N287_V0.5510.76
173_G176_S0.5490.76
248_A518_T0.5470.76
18_G25_P0.5470.76
534_V539_V0.5460.76
534_V538_G0.5450.76
51_A58_A0.5450.76
435_V478_D0.5440.75
314_G445_R0.5440.75
409_Y413_G0.5430.75
421_T426_P0.5430.75
193_C424_A0.5430.75
170_S221_L0.5420.75
27_T30_G0.5400.75
449_A485_R0.5400.75
160_A172_S0.5380.75
451_I477_Y0.5380.75
254_G258_P0.5370.75
294_D354_P0.5370.75
159_D162_H0.5360.74
254_G309_K0.5360.74
175_F237_A0.5350.74
445_R449_A0.5350.74
146_G216_P0.5350.74
152_A223_M0.5350.74
319_S365_V0.5350.74
156_S191_T0.5350.74
18_G23_T0.5340.74
154_V253_L0.5330.74
170_S177_L0.5330.74
164_D415_Y0.5310.74
515_F521_Y0.5300.74
79_D82_P0.5300.74
483_E516_D0.5290.73
201_G204_A0.5290.73
538_G548_L0.5280.73
534_V546_E0.5280.73
174_N178_D0.5270.73
16_I20_S0.5260.73
160_A183_T0.5250.73
25_P38_V0.5250.73
481_T484_D0.5240.73
193_C423_M0.5240.73
70_E76_Y0.5240.73
295_T307_T0.5240.73
234_I272_Y0.5230.73
481_T517_G0.5230.73
160_A165_L0.5230.73
156_S188_S0.5220.72
122_P127_L0.5220.72
379_S413_G0.5200.72
106_P238_A0.5200.72
539_V549_Y0.5190.72
503_V507_I0.5190.72
124_P128_Q0.5170.72
307_T310_G0.5170.72
199_A202_A0.5160.72
121_I125_H0.5150.71
379_S411_G0.5150.71
349_S353_T0.5150.71
536_G545_V0.5150.71
522_P543_A0.5150.71
182_G186_D0.5140.71
463_Q466_Y0.5130.71
367_V455_A0.5130.71
199_A210_Y0.5130.71
539_V548_L0.5120.71
235_L272_Y0.5110.71
317_S364_S0.5100.71
57_E63_F0.5100.71
155_D190_G0.5100.71
140_H212_V0.5090.71
23_T28_A0.5090.71
521_Y525_P0.5090.71
533_D536_G0.5090.71
277_E312_H0.5080.70
55_I64_A0.5070.70
523_A529_G0.5070.70
242_I245_G0.5070.70
50_E53_D0.5050.70
251_L309_K0.5040.70
46_D49_R0.5030.70
415_Y423_M0.5020.70
15_A21_A0.5020.70
15_A19_L0.5000.69
32_R38_V0.5000.69
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3whiA 2 0.6034 100 0.407 Contact Map
3afgA 1 0.8085 100 0.411 Contact Map
3wiuA 2 0.5983 100 0.423 Contact Map
1xf1A 2 0.6974 100 0.423 Contact Map
1r6vA 1 0.8462 100 0.425 Contact Map
3t41A 1 0.6615 100 0.428 Contact Map
4tr2A 2 0.6325 100 0.432 Contact Map
3vtaA 1 0.694 100 0.448 Contact Map
2p4eP 2 0.7385 100 0.452 Contact Map
3i6sA 2 0.6991 100 0.454 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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