GREMLIN Database
NUCS2 - Endonuclease NucS 2
UniProt: Q9HNR1 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 250 (215)
Sequences: 460 (190)
Seq/√Len: 12.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
141_P164_F5.8161.00
206_R228_E5.3911.00
62_L68_G4.5351.00
195_V227_L4.4751.00
141_P166_A3.7600.99
140_N237_L3.7080.99
62_L67_T3.1410.97
61_F105_T2.9330.96
48_D108_H2.5770.92
37_E45_Y2.5390.91
143_A237_L2.5110.91
53_L63_V2.4980.91
97_S101_E2.4760.90
132_E135_E2.4750.90
33_R50_D2.4600.90
62_L65_Q2.4580.90
188_F218_V2.3760.88
30_V53_L2.3500.88
168_D206_R2.3130.87
144_L176_I2.2170.84
141_P232_L2.2000.84
144_L232_L2.1860.84
141_P148_L2.0560.79
140_N143_A2.0380.79
141_P178_V1.9570.76
76_P120_D1.9180.74
165_Y173_P1.9060.74
143_A148_L1.8550.72
143_A236_G1.8100.70
50_D111_Y1.7770.68
184_T215_S1.7340.66
65_Q68_G1.7270.66
176_I208_M1.7090.65
96_R101_E1.6990.65
112_G116_F1.6810.64
96_R99_P1.6500.62
29_S52_L1.6240.61
54_V67_T1.6240.61
208_M228_E1.6130.61
223_R228_E1.6020.60
33_R108_H1.5630.58
148_L166_A1.5130.56
144_L148_L1.5100.55
145_E148_L1.4990.55
86_S101_E1.4680.53
156_E198_Y1.4670.53
145_E206_R1.4380.52
148_L164_F1.4350.51
92_L96_R1.4320.51
32_A107_L1.4300.51
181_I213_S1.4220.51
164_F178_V1.4180.51
92_L104_E1.3740.48
92_L95_R1.3640.48
53_L105_T1.3450.47
76_P99_P1.3010.45
101_E106_R1.2990.44
132_E184_T1.2960.44
164_F232_L1.2760.43
97_S105_T1.2670.43
66_P181_I1.2570.42
147_G168_D1.2560.42
46_L124_Y1.2250.41
34_C46_L1.2180.40
199_A205_V1.2140.40
53_L98_N1.2130.40
95_R100_T1.1870.39
87_Q110_V1.1650.38
148_L178_V1.1580.37
109_E142_D1.1480.37
92_L101_E1.1440.37
62_L98_N1.1410.37
142_D146_A1.1390.36
232_L237_L1.1340.36
44_G75_M1.1300.36
86_S104_E1.1280.36
60_T210_V1.1260.36
151_V165_Y1.1150.35
156_E165_Y1.1060.35
103_V221_A1.1050.35
54_V68_G1.0930.34
92_L99_P1.0860.34
166_A178_V1.0830.34
104_E135_E1.0780.34
166_A173_P1.0740.33
235_F238_D1.0650.33
128_G193_R1.0500.32
93_L99_P1.0280.31
198_Y232_L1.0200.31
144_L228_E1.0140.31
39_N102_R1.0130.31
116_F184_T1.0070.30
50_D115_R1.0060.30
199_A204_D1.0030.30
236_G240_K0.9970.30
93_L96_R0.9930.30
82_E203_D0.9810.29
200_E224_D0.9740.29
53_L107_L0.9740.29
85_T88_G0.9730.29
76_P184_T0.9730.29
151_V173_P0.9660.29
112_G240_K0.9600.28
52_L98_N0.9600.28
36_V46_L0.9430.28
140_N240_K0.9420.28
146_A169_G0.9390.27
96_R240_K0.9380.27
141_P199_A0.9320.27
36_V64_H0.9300.27
32_A53_L0.9250.27
187_H209_L0.9220.27
69_H189_D0.9210.27
164_F199_A0.9120.26
90_A166_A0.9070.26
84_R93_L0.9060.26
208_M215_S0.9030.26
69_H78_G0.9010.26
219_T226_G0.8990.26
27_V37_E0.8940.26
159_Y197_R0.8910.25
76_P96_R0.8910.25
101_E108_H0.8850.25
31_Q107_L0.8810.25
59_G64_H0.8810.25
96_R141_P0.8800.25
183_A209_L0.8800.25
131_A134_H0.8790.25
219_T223_R0.8650.24
30_V36_V0.8640.24
175_V201_T0.8590.24
86_S92_L0.8540.24
68_G96_R0.8510.24
67_T92_L0.8510.24
141_P204_D0.8470.24
105_T233_A0.8460.24
43_S140_N0.8440.24
148_L206_R0.8380.23
72_V120_D0.8370.23
110_V140_N0.8340.23
95_R101_E0.8260.23
69_H129_T0.8250.23
149_R167_V0.8160.23
39_N104_E0.8140.23
48_D125_E0.8140.23
96_R213_S0.8100.22
36_V59_G0.8090.22
172_T206_R0.8060.22
166_A176_I0.8050.22
50_D109_E0.8000.22
146_A156_E0.7980.22
92_L223_R0.7980.22
206_R230_V0.7970.22
32_A185_L0.7920.22
87_Q169_G0.7910.22
88_G171_G0.7910.22
76_P227_L0.7860.22
168_D172_T0.7800.21
134_H153_H0.7720.21
54_V76_P0.7660.21
62_L93_L0.7650.21
133_M161_F0.7550.20
86_S91_V0.7530.20
44_G212_P0.7430.20
131_A137_I0.7390.20
50_D120_D0.7330.20
33_R109_E0.7300.20
148_L176_I0.7290.20
43_S135_E0.7230.19
86_S106_R0.7230.19
186_N201_T0.7230.19
50_D232_L0.7190.19
166_A232_L0.7190.19
118_A226_G0.7160.19
142_D149_R0.7150.19
64_H195_V0.7140.19
120_D242_A0.7070.19
92_L170_D0.7060.19
93_L97_S0.7060.19
144_L164_F0.7060.19
46_L51_R0.7060.19
67_T96_R0.7030.19
100_T209_L0.7030.19
49_G75_M0.7000.19
119_E122_A0.7000.19
96_R184_T0.6990.19
39_N101_E0.6990.19
148_L232_L0.6970.19
42_T126_E0.6970.19
157_T162_I0.6960.19
114_T146_A0.6940.18
196_D232_L0.6940.18
55_A106_R0.6930.18
173_P198_Y0.6910.18
167_V173_P0.6900.18
124_Y181_I0.6870.18
49_G72_V0.6870.18
149_R203_D0.6760.18
60_T69_H0.6650.18
183_A187_H0.6650.18
129_T201_T0.6630.18
143_A166_A0.6620.17
186_N218_V0.6580.17
146_A170_D0.6570.17
35_E48_D0.6570.17
95_R102_R0.6560.17
85_T156_E0.6550.17
95_R99_P0.6550.17
131_A162_I0.6550.17
173_P232_L0.6530.17
43_S237_L0.6520.17
125_E184_T0.6510.17
102_R106_R0.6480.17
128_G162_I0.6480.17
38_Y73_N0.6470.17
101_E140_N0.6470.17
39_N114_T0.6460.17
96_R172_T0.6450.17
162_I221_A0.6440.17
100_T165_Y0.6440.17
126_E234_E0.6420.17
31_Q176_I0.6400.17
192_K225_N0.6360.17
53_L77_G0.6360.17
157_T186_N0.6330.17
114_T170_D0.6320.17
173_P203_D0.6310.17
55_A206_R0.6300.17
50_D101_E0.6290.17
49_G120_D0.6290.17
95_R105_T0.6260.16
49_G78_G0.6230.16
99_P242_A0.6200.16
156_E205_V0.6200.16
48_D105_T0.6200.16
59_G218_V0.6190.16
170_D236_G0.6190.16
78_G185_L0.6180.16
93_L102_R0.6180.16
96_R209_L0.6180.16
62_L94_A0.6160.16
59_G76_P0.6140.16
124_Y128_G0.6120.16
94_A99_P0.6110.16
85_T120_D0.6100.16
145_E168_D0.5980.16
86_S241_G0.5980.16
157_T161_F0.5970.16
47_G164_F0.5950.16
55_A149_R0.5950.16
66_P124_Y0.5950.16
110_V175_V0.5940.16
30_V81_V0.5930.15
70_K119_E0.5900.15
91_V97_S0.5890.15
38_Y41_R0.5890.15
96_R206_R0.5880.15
104_E236_G0.5870.15
123_T219_T0.5870.15
35_E236_G0.5840.15
39_N43_S0.5840.15
82_E86_S0.5750.15
87_Q149_R0.5720.15
106_R149_R0.5670.15
107_L111_Y0.5660.15
82_E170_D0.5650.15
83_A106_R0.5630.15
237_L240_K0.5610.15
135_E238_D0.5610.15
32_A195_V0.5610.15
55_A61_F0.5600.15
206_R213_S0.5560.15
61_F238_D0.5550.14
152_E157_T0.5540.14
148_L228_E0.5540.14
33_R48_D0.5530.14
64_H181_I0.5510.14
134_H138_E0.5480.14
136_Y181_I0.5430.14
119_E225_N0.5430.14
39_N98_N0.5380.14
132_E178_V0.5370.14
93_L101_E0.5370.14
48_D169_G0.5370.14
46_L189_D0.5360.14
36_V54_V0.5350.14
90_A206_R0.5340.14
142_D241_G0.5330.14
39_N106_R0.5320.14
114_T171_G0.5300.14
146_A204_D0.5300.14
43_S143_A0.5280.14
77_G118_A0.5280.14
89_D108_H0.5260.14
82_E169_G0.5260.14
34_C51_R0.5260.14
39_N135_E0.5250.14
90_A110_V0.5220.14
79_G149_R0.5210.14
93_L105_T0.5200.14
184_T242_A0.5200.14
80_T120_D0.5180.14
156_E220_R0.5160.14
157_T221_A0.5150.13
135_E167_V0.5140.13
73_N221_A0.5120.13
172_T184_T0.5110.13
161_F217_R0.5100.13
27_V219_T0.5090.13
144_L226_G0.5090.13
131_A205_V0.5080.13
48_D93_L0.5070.13
135_E184_T0.5070.13
159_Y176_I0.5050.13
92_L102_R0.5050.13
126_E134_H0.5030.13
31_Q45_Y0.5030.13
209_L215_S0.5020.13
79_G199_A0.5010.13
139_A236_G0.5010.13
78_G217_R0.5010.13
70_K225_N0.5000.13
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2vldA 2 0.904 100 0.26 Contact Map
1y88A 5 0.46 97 0.925 Contact Map
2wcwA 2 0.428 96.2 0.932 Contact Map
3h1tA 1 0.428 94.2 0.94 Contact Map
1gefA 2 0.42 94.2 0.94 Contact Map
2eo0A 2 0.424 92.8 0.943 Contact Map
1hh1A 2 0.412 91.9 0.945 Contact Map
1ob8A 1 0.412 91.1 0.946 Contact Map
4qbnA 2 0.372 78.1 0.955 Contact Map
3fovA 2 0.38 75.6 0.955 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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