GREMLIN Database
NADE - NH(3)-dependent NAD(+) synthetase
UniProt: Q9HNM7 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 268 (246)
Sequences: 4526 (2580)
Seq/√Len: 164.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
192_E196_T4.9101.00
71_L138_V3.8061.00
59_T147_L3.5111.00
41_R144_G3.4531.00
23_R187_D2.8971.00
253_D257_A2.8811.00
88_R193_D2.7241.00
70_G95_I2.5341.00
54_T185_A2.4041.00
245_P249_E2.4021.00
34_V42_C2.3571.00
30_I58_L2.2821.00
20_A187_D2.2711.00
250_T254_L2.2091.00
80_D84_S2.1681.00
85_D197_K2.1071.00
46_L90_A2.0991.00
90_A97_Y2.0671.00
56_A95_I2.0251.00
55_V173_I1.9711.00
87_E91_E1.9431.00
45_G135_N1.9371.00
109_L133_V1.8481.00
103_D107_T1.8291.00
19_L183_Q1.8261.00
259_K263_A1.8261.00
130_T133_V1.8081.00
23_R61_D1.7191.00
76_A103_D1.7131.00
226_V232_L1.6991.00
42_C63_L1.6921.00
50_I185_A1.6901.00
85_D193_D1.6651.00
234_A249_E1.6261.00
88_R91_E1.6231.00
69_H98_D1.5851.00
72_V97_Y1.5821.00
183_Q186_R1.5791.00
186_R192_E1.5721.00
182_R218_D1.5571.00
28_S32_D1.5211.00
138_V142_G1.4961.00
160_Y166_Q1.4831.00
186_R195_V1.4761.00
37_A145_V1.4761.00
87_E99_V1.4681.00
81_Q193_D1.4681.00
53_T194_L1.4541.00
69_H144_G1.4531.00
42_C59_T1.4381.00
219_T247_V1.4321.00
24_D65_A1.4291.00
208_T211_G1.4291.00
126_A133_V1.4201.00
226_V237_T1.4191.00
20_A24_D1.4161.00
109_L130_T1.4101.00
198_A208_T1.4071.00
41_R69_H1.4041.00
177_Y180_Q1.4031.00
88_R92_D1.4021.00
216_D219_T1.3711.00
41_R67_A1.3611.00
210_A216_D1.3601.00
157_T220_I1.3431.00
40_E143_D1.3311.00
157_T213_L1.3271.00
106_V130_T1.3221.00
84_S193_D1.3211.00
50_I181_V1.3211.00
89_V194_L1.3131.00
58_L172_P1.3101.00
60_V65_A1.3001.00
225_A230_G1.2931.00
104_P111_D1.2921.00
122_A137_F1.2921.00
152_R175_N1.2791.00
72_V83_M1.2771.00
56_A70_G1.2741.00
168_V171_N1.2691.00
30_I42_C1.2661.00
42_C147_L1.2591.00
57_H61_D1.2491.00
232_L236_A1.2441.00
179_M222_A1.2391.00
31_R65_A1.2371.00
254_L258_S1.2361.00
222_A241_L1.2211.00
56_A93_L1.2091.00
61_D188_L1.2021.00
105_F112_V1.2001.00
219_T244_D1.1971.00
254_L257_A1.1871.00
222_A243_I1.1861.00
87_E97_Y1.1851.00
162_K260_H1.1771.00
224_L255_Y1.1751.00
55_V147_L1.1711.00
43_V138_V1.1621.00
33_T36_A1.1581.00
226_V241_L1.1541.00
23_R188_L1.1521.00
34_V63_L1.1321.00
244_D247_V1.1321.00
177_Y224_L1.1291.00
84_S88_R1.1251.00
72_V86_A1.1251.00
186_R196_T1.1201.00
60_V93_L1.1151.00
176_L180_Q1.1151.00
70_G90_A1.1001.00
258_S262_R1.1001.00
24_D28_S1.0941.00
23_R27_Q1.0881.00
60_V68_L1.0791.00
98_D142_G1.0730.99
250_T253_D1.0720.99
31_R35_A1.0710.99
201_A207_Q1.0700.99
76_A106_V1.0610.99
107_T110_T1.0610.99
108_Q111_D1.0600.99
81_Q200_T1.0570.99
179_M225_A1.0510.99
78_S81_Q1.0510.99
108_Q112_V1.0460.99
23_R58_L1.0450.99
163_Y167_A1.0410.99
166_Q265_P1.0350.99
151_N209_D1.0320.99
75_G106_V1.0310.99
64_G68_L1.0310.99
157_T254_L1.0270.99
105_F130_T1.0240.99
105_F134_I1.0180.99
249_E253_D1.0140.99
27_Q31_R1.0130.99
238_A245_P1.0070.99
148_G168_V1.0070.99
106_V110_T0.9950.99
226_V230_G0.9940.99
72_V87_E0.9850.99
171_N174_G0.9840.99
147_L170_C0.9840.99
83_M99_V0.9760.99
34_V37_A0.9730.99
57_H93_L0.9720.99
201_A209_D0.9710.99
150_G160_Y0.9710.99
259_K262_R0.9660.99
50_I195_V0.9640.99
204_W212_E0.9560.99
105_F108_Q0.9550.99
30_I170_C0.9530.99
246_S250_T0.9520.99
27_Q62_E0.9510.99
233_P236_A0.9500.99
234_A238_A0.9410.99
83_M87_E0.9380.99
60_V64_G0.9350.99
92_D190_V0.9340.99
132_A167_A0.9310.99
26_I58_L0.9290.99
182_R198_A0.9280.99
35_A67_A0.9260.98
117_A141_H0.9260.98
105_F109_L0.9250.98
107_T111_D0.9240.98
32_D36_A0.9240.98
238_A248_V0.9230.98
73_M134_I0.9220.98
234_A252_R0.9160.98
123_V141_H0.9110.98
70_G97_Y0.9110.98
232_L241_L0.9080.98
152_R229_D0.9030.98
122_A141_H0.8900.98
162_K169_D0.8840.98
102_I130_T0.8760.98
100_V112_V0.8680.98
113_F137_F0.8670.98
180_Q183_Q0.8580.98
87_E90_A0.8570.97
207_Q212_E0.8560.97
81_Q197_K0.8550.97
76_A107_T0.8460.97
44_L55_V0.8390.97
169_D260_H0.8350.97
26_I175_N0.8320.97
63_L67_A0.8320.97
22_R180_Q0.8260.97
27_Q61_D0.8220.97
100_V134_I0.8160.97
222_A226_V0.8130.97
60_V95_I0.8010.96
53_T89_V0.7990.96
75_G107_T0.7980.96
40_E67_A0.7980.96
130_T134_I0.7970.96
146_V167_A0.7960.96
41_R142_G0.7910.96
185_A195_V0.7890.96
125_N161_T0.7850.96
84_S87_E0.7820.96
159_Y201_A0.7790.96
45_G73_M0.7770.96
136_Y169_D0.7770.96
199_P209_D0.7760.96
19_L187_D0.7760.96
61_D64_G0.7750.95
177_Y225_A0.7650.95
159_Y212_E0.7650.95
115_D118_G0.7600.95
155_A174_G0.7560.95
43_V144_G0.7550.95
69_H96_E0.7550.95
28_S31_R0.7500.95
82_N202_E0.7470.94
104_P112_V0.7460.94
221_D224_L0.7430.94
63_L68_L0.7420.94
155_A160_Y0.7380.94
220_I251_V0.7360.94
181_V185_A0.7360.94
72_V99_V0.7350.94
30_I172_P0.7330.94
44_L68_L0.7290.94
124_G212_E0.7260.94
170_C174_G0.7250.94
101_E104_P0.7230.93
22_R26_I0.7230.93
207_Q211_G0.7170.93
151_N199_P0.7140.93
34_V41_R0.7130.93
59_T68_L0.7070.93
126_A137_F0.7060.93
30_I63_L0.7020.92
137_F141_H0.7010.92
151_N201_A0.7000.92
39_A143_D0.6980.92
247_V250_T0.6960.92
73_M135_N0.6890.92
69_H142_G0.6860.92
109_L113_F0.6850.92
44_L56_A0.6830.91
159_Y207_Q0.6780.91
122_A140_N0.6780.91
246_S249_E0.6760.91
125_N129_R0.6750.91
112_V115_D0.6700.91
182_R195_V0.6700.91
163_Y168_V0.6670.90
73_M146_V0.6640.90
117_A142_G0.6600.90
84_S91_E0.6590.90
81_Q86_A0.6580.90
57_H92_D0.6560.90
54_T181_V0.6520.89
57_H64_G0.6510.89
238_A241_L0.6510.89
148_G167_A0.6490.89
139_A260_H0.6490.89
119_D141_H0.6460.89
88_R191_P0.6420.89
127_R202_E0.6370.88
153_A220_I0.6360.88
179_M221_D0.6340.88
146_V260_H0.6320.88
179_M218_D0.6300.88
252_R256_G0.6280.88
208_T212_E0.6270.88
174_G177_Y0.6270.88
89_V92_D0.6270.88
75_G102_I0.6240.87
47_S51_D0.6240.87
248_V252_R0.6220.87
75_G130_T0.6200.87
150_G166_Q0.6200.87
37_A174_G0.6190.87
57_H89_V0.6190.87
64_G96_E0.6170.87
113_F117_A0.6170.87
110_T114_P0.6170.87
223_V248_V0.6160.87
119_D122_A0.6150.87
182_R186_R0.6130.86
104_P107_T0.6130.86
71_L134_I0.6130.86
210_A218_D0.6110.86
256_G259_K0.6110.86
90_A95_I0.6110.86
32_D35_A0.6090.86
100_V116_A0.6090.86
155_A171_N0.6090.86
214_G217_Y0.6060.86
57_H190_V0.6040.86
235_S245_P0.6020.85
152_R174_G0.6020.85
243_I248_V0.5960.85
102_I106_V0.5950.85
231_G234_A0.5930.84
21_E25_R0.5930.84
42_C170_C0.5930.84
233_P237_T0.5900.84
31_R62_E0.5860.84
22_R25_R0.5820.83
24_D27_Q0.5820.83
219_T222_A0.5800.83
150_G171_N0.5800.83
18_E21_E0.5780.83
120_E123_V0.5780.83
243_I247_V0.5770.83
96_E144_G0.5730.82
198_A210_A0.5710.82
220_I223_V0.5690.82
126_A130_T0.5680.82
109_L112_V0.5680.82
109_L126_A0.5650.82
98_D138_V0.5640.81
70_G194_L0.5610.81
100_V113_F0.5600.81
46_L89_V0.5590.81
222_A225_A0.5580.81
183_Q187_D0.5550.80
124_G204_W0.5540.80
223_V252_R0.5500.80
54_T188_L0.5490.80
98_D116_A0.5480.80
157_T251_V0.5450.79
139_A144_G0.5440.79
78_S194_L0.5430.79
128_A161_T0.5430.79
121_V124_G0.5420.79
56_A89_V0.5420.79
42_C145_V0.5400.79
111_D114_P0.5370.78
57_H60_V0.5340.78
150_G168_V0.5340.78
125_N128_A0.5320.78
82_N197_K0.5320.78
235_S238_A0.5320.78
155_A265_P0.5320.78
98_D101_E0.5310.77
173_I181_V0.5310.77
50_I178_K0.5300.77
148_G172_P0.5250.77
78_S202_E0.5250.77
72_V78_S0.5240.77
124_G128_A0.5240.77
249_E256_G0.5230.76
106_V126_A0.5190.76
150_G155_A0.5190.76
223_V237_T0.5160.75
75_G103_D0.5140.75
75_G127_R0.5140.75
148_G160_Y0.5140.75
155_A258_S0.5130.75
79_R197_K0.5120.75
41_R143_D0.5100.75
132_A136_Y0.5090.74
30_I144_G0.5090.74
111_D115_D0.5060.74
219_T223_V0.5050.74
124_G127_R0.5050.74
57_H188_L0.5040.74
50_I182_R0.5040.74
167_A171_N0.5020.73
162_K166_Q0.5010.73
128_A203_L0.5000.73
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3ilvA 2 0.9142 100 0.346 Contact Map
1kqpA 2 0.9328 100 0.368 Contact Map
3q4gA 2 0.8993 100 0.373 Contact Map
4xfdA 1 0.8806 100 0.373 Contact Map
3n05A 2 0.9179 100 0.375 Contact Map
1wxiA 2 0.8993 100 0.375 Contact Map
4f4hA 2 0.9328 100 0.378 Contact Map
4q16A 2 0.9328 100 0.378 Contact Map
1xngA 2 0.9254 100 0.378 Contact Map
3dpiA 3 0.9104 100 0.391 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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