GREMLIN Database
RIFK - Riboflavin kinase
UniProt: Q9HNF4 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 233 (213)
Sequences: 161 (124)
Seq/√Len: 8.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
102_L143_L3.4360.94
21_L26_A3.1020.91
90_Y94_F2.9740.89
132_Y193_H2.8430.86
115_H165_W2.7450.84
174_S198_D2.6520.82
127_A133_E2.5280.79
129_K163_D2.4250.76
159_G178_Y2.3240.72
35_C202_H2.0950.64
23_L94_F2.0900.64
48_T51_R2.0870.64
108_S114_R2.0480.62
222_L225_D2.0350.61
19_K86_E2.0280.61
23_L90_Y2.0270.61
21_L172_Y2.0240.61
194_V221_L1.9840.59
177_C206_Q1.9540.58
71_Q86_E1.9240.57
42_L52_R1.8780.55
53_L203_D1.8490.54
125_Q129_K1.8200.53
45_S48_T1.8020.52
195_I203_D1.7800.51
86_E90_Y1.7590.50
71_Q126_F1.7450.49
34_S117_I1.7400.49
91_R141_V1.7210.48
100_L198_D1.7180.48
115_H202_H1.7080.48
216_R223_D1.6940.47
70_G147_G1.6510.45
126_F177_C1.6210.44
164_A209_I1.6160.44
33_V182_V1.6140.44
102_L117_I1.5930.43
49_A125_Q1.5770.42
106_V221_L1.5470.41
123_N133_E1.4790.38
26_A161_D1.4770.38
167_D178_Y1.4670.38
84_K93_I1.4650.38
75_V169_D1.4550.37
172_Y197_P1.4470.37
114_R175_A1.4450.37
45_S51_R1.4430.37
159_G176_T1.4410.37
191_G205_D1.4290.36
160_V210_I1.4220.36
42_L47_Q1.4210.36
36_G157_L1.4030.35
35_C71_Q1.3950.35
22_A25_G1.3660.34
49_A90_Y1.3590.34
31_V231_V1.3560.34
83_L197_P1.3550.34
125_Q177_C1.3500.33
26_A84_K1.3490.33
49_A71_Q1.3440.33
214_K217_E1.3380.33
32_K67_V1.3320.33
143_L151_R1.3290.33
45_S86_E1.3230.32
47_Q56_L1.3000.32
47_Q50_S1.2920.31
42_L50_S1.2890.31
71_Q125_Q1.2770.31
21_L58_D1.2760.31
159_G180_C1.2700.30
160_V211_A1.2700.30
60_D73_I1.2540.30
93_I209_I1.2480.30
86_E172_Y1.2400.29
94_F201_H1.2390.29
17_V224_D1.2370.29
217_E229_I1.2270.29
179_A192_A1.2090.28
143_L177_C1.2080.28
163_D195_I1.2070.28
28_R61_H1.1840.27
121_G201_H1.1800.27
35_C115_H1.1790.27
142_D192_A1.1780.27
225_D229_I1.1760.27
154_I231_V1.1760.27
217_E222_L1.1690.27
35_C165_W1.1690.27
46_S206_Q1.1630.27
115_H172_Y1.1490.26
216_R221_L1.1460.26
69_D135_Y1.1440.26
184_G221_L1.1410.26
172_Y202_H1.1330.26
63_T227_V1.1140.25
86_E202_H1.1050.25
33_V196_I1.1040.25
36_G198_D1.0960.25
78_A227_V1.0930.25
39_A231_V1.0930.25
117_I198_D1.0890.24
66_L176_T1.0870.24
143_L201_H1.0840.24
62_V126_F1.0830.24
130_L223_D1.0720.24
215_L219_L1.0660.24
102_L175_A1.0660.24
191_G204_D1.0600.24
58_D227_V1.0570.23
67_V72_W1.0570.23
164_A192_A1.0480.23
76_T87_Y1.0400.23
18_L79_G1.0290.23
102_L167_D1.0290.23
166_E190_D1.0180.22
172_Y209_I1.0100.22
90_Y175_A1.0090.22
19_K71_Q1.0090.22
34_S86_E1.0020.22
25_G143_L1.0020.22
172_Y203_D0.9990.22
192_A212_P0.9970.22
38_L47_Q0.9970.22
119_L124_R0.9930.22
75_V79_G0.9890.21
124_R144_P0.9830.21
141_V209_I0.9790.21
135_Y182_V0.9750.21
46_S50_S0.9730.21
49_A64_R0.9630.21
19_K93_I0.9610.21
45_S197_P0.9600.21
31_V75_V0.9600.21
33_V44_A0.9560.21
64_R143_L0.9560.21
77_D209_I0.9550.20
164_A198_D0.9520.20
51_R197_P0.9490.20
102_L147_G0.9480.20
68_S225_D0.9460.20
119_L151_R0.9400.20
52_R56_L0.9350.20
141_V200_T0.9340.20
53_L143_L0.9320.20
19_K90_Y0.9290.20
45_S49_A0.9280.20
39_A47_Q0.9240.20
178_Y190_D0.9170.19
35_C50_S0.9120.19
71_Q93_I0.9090.19
54_Q202_H0.8910.19
64_R126_F0.8900.19
86_E197_P0.8890.19
215_L223_D0.8860.19
107_T142_D0.8820.19
29_G70_G0.8760.18
48_T177_C0.8760.18
106_V169_D0.8710.18
73_I106_V0.8660.18
55_A102_L0.8610.18
208_E216_R0.8500.18
132_Y203_D0.8490.18
177_C195_I0.8460.18
86_E93_I0.8390.17
135_Y214_K0.8390.17
49_A87_Y0.8340.17
178_Y211_A0.8330.17
117_I141_V0.8300.17
120_P190_D0.8300.17
125_Q171_T0.8280.17
19_K23_L0.8270.17
67_V102_L0.8220.17
100_L207_L0.8200.17
17_V21_L0.8170.17
135_Y222_L0.8160.17
75_V181_T0.8130.17
178_Y207_L0.8120.17
43_D65_D0.8120.17
50_S192_A0.8120.17
99_E194_V0.8110.17
44_A59_A0.8100.17
57_D62_V0.8080.17
69_D80_R0.8080.17
143_L175_A0.8020.17
71_Q87_Y0.8010.17
86_E114_R0.7890.16
139_L221_L0.7830.16
56_L70_G0.7820.16
110_M203_D0.7800.16
86_E125_Q0.7800.16
66_L100_L0.7790.16
36_G155_Q0.7770.16
53_L160_V0.7710.16
135_Y168_E0.7660.16
132_Y177_C0.7640.16
27_H159_G0.7640.16
132_Y195_I0.7520.15
34_S38_L0.7510.15
22_A51_R0.7470.15
191_G226_E0.7370.15
154_I229_I0.7370.15
53_L70_G0.7370.15
81_H92_R0.7350.15
75_V85_H0.7300.15
41_R82_A0.7280.15
176_T196_I0.7280.15
37_D152_A0.7240.15
174_S197_P0.7170.15
20_L46_S0.7170.15
119_L211_A0.7150.15
164_A172_Y0.7150.15
83_L223_D0.7140.15
45_S172_Y0.7110.15
44_A172_Y0.7060.14
36_G42_L0.6970.14
21_L28_R0.6960.14
192_A209_I0.6960.14
36_G159_G0.6940.14
145_P205_D0.6900.14
73_I108_S0.6870.14
73_I200_T0.6840.14
27_H205_D0.6810.14
60_D106_V0.6810.14
110_M132_Y0.6740.14
114_R213_V0.6660.14
61_H87_Y0.6580.13
30_E124_R0.6570.13
80_R190_D0.6570.13
133_E211_A0.6560.13
39_A42_L0.6560.13
119_L171_T0.6540.13
54_Q70_G0.6530.13
220_G225_D0.6520.13
48_T197_P0.6510.13
142_D202_H0.6490.13
102_L177_C0.6410.13
174_S191_G0.6370.13
154_I172_Y0.6370.13
48_T143_L0.6360.13
181_T191_G0.6340.13
21_L66_L0.6340.13
31_V157_L0.6330.13
101_A135_Y0.6260.13
22_A90_Y0.6260.13
133_E141_V0.6260.13
143_L172_Y0.6220.13
82_A231_V0.6190.13
132_Y163_D0.6170.13
208_E215_L0.6170.13
85_H89_D0.6130.13
127_A182_V0.6110.13
49_A86_E0.6050.12
135_Y166_E0.6020.12
91_R95_E0.6000.12
129_K193_H0.6000.12
144_P201_H0.5970.12
15_L196_I0.5970.12
95_E181_T0.5950.12
75_V123_N0.5930.12
57_D136_P0.5910.12
33_V77_D0.5910.12
141_V230_R0.5850.12
19_K197_P0.5830.12
60_D162_I0.5810.12
31_V163_D0.5780.12
128_E164_A0.5770.12
129_K160_V0.5740.12
83_L111_G0.5720.12
50_S114_R0.5710.12
59_A110_M0.5710.12
195_I221_L0.5690.12
118_S131_G0.5650.12
29_G181_T0.5620.12
117_I129_K0.5620.12
116_Y172_Y0.5580.12
61_H166_E0.5580.12
88_E218_R0.5570.12
200_T211_A0.5560.12
107_T206_Q0.5540.12
116_Y164_A0.5540.12
50_S54_Q0.5530.12
22_A94_F0.5510.12
116_Y171_T0.5490.11
136_P203_D0.5480.11
160_V229_I0.5480.11
78_A146_D0.5470.11
74_T128_E0.5390.11
75_V81_H0.5370.11
15_L22_A0.5360.11
36_G224_D0.5350.11
108_S192_A0.5330.11
57_D64_R0.5320.11
194_V198_D0.5320.11
69_D94_F0.5300.11
69_D151_R0.5290.11
61_H98_G0.5270.11
124_R229_I0.5260.11
94_F147_G0.5260.11
62_V131_G0.5250.11
71_Q90_Y0.5240.11
58_D196_I0.5230.11
105_T222_L0.5210.11
59_A120_P0.5200.11
123_N127_A0.5190.11
88_E153_G0.5130.11
75_V127_A0.5120.11
150_A203_D0.5110.11
222_L230_R0.5100.11
116_Y165_W0.5090.11
212_P231_V0.5040.11
83_L219_L0.5030.11
61_H205_D0.5030.11
78_A229_I0.5020.11
95_E125_Q0.5020.11
132_Y194_V0.5010.11
48_T71_Q0.5010.11
95_E202_H0.5010.11
82_A183_V0.5000.11
61_H85_H0.5000.11
58_D157_L0.5000.11
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2oynA 2 0.5708 100 0.484 Contact Map
3ctaA 2 0.8026 100 0.717 Contact Map
2p8tA 1 0.6652 99.8 0.792 Contact Map
2obpA 1 0.3348 99.1 0.871 Contact Map
1hsjA 2 0.3948 99 0.875 Contact Map
4l9nA 2 0.5064 98.9 0.882 Contact Map
1p4xA 1 0.4249 98.8 0.883 Contact Map
2qvoA 2 0.3734 98.8 0.883 Contact Map
3e6mA 2 0.4893 98.8 0.885 Contact Map
4asnA 2 0.3777 98.8 0.886 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0078 seconds.