GREMLIN Database
MDH - Malate dehydrogenase
UniProt: Q9HMV8 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 304 (295)
Sequences: 4927 (2902)
Seq/√Len: 169.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
5_S72_S4.2561.00
74_V234_V2.9761.00
206_A210_E2.9501.00
286_R290_D2.8601.00
76_V234_V2.6491.00
81_I95_D2.4781.00
119_T230_V2.4041.00
5_S69_T2.3811.00
97_A129_H2.3601.00
197_S200_E2.3291.00
24_R235_E2.3151.00
69_T111_N2.3081.00
242_G269_G2.2551.00
257_L289_L2.2231.00
242_G274_E2.1881.00
207_D214_N2.1511.00
138_R252_D2.1451.00
106_S109_E2.1361.00
262_L289_L2.1311.00
269_G274_E2.0951.00
294_E298_A2.0921.00
104_G130_L2.0261.00
203_E207_D1.9711.00
108_A136_R1.9491.00
131_Y261_G1.9241.00
267_K275_E1.9231.00
101_E105_S1.9221.00
108_A135_D1.9151.00
266_V273_V1.9141.00
104_G134_G1.8811.00
173_L183_V1.8711.00
102_D106_S1.8511.00
262_L293_A1.8421.00
170_A187_V1.8371.00
161_F189_V1.8351.00
105_S133_S1.8331.00
115_V237_I1.8231.00
3_K31_V1.8191.00
290_D294_E1.8031.00
287_D291_E1.7841.00
116_T130_L1.7741.00
93_A299_Q1.7731.00
257_L290_D1.7601.00
251_L264_V1.7521.00
160_R200_E1.7391.00
249_M266_V1.7351.00
198_A202_E1.7311.00
3_K29_E1.7211.00
4_V27_V1.7041.00
136_R140_S1.6991.00
283_A286_R1.6391.00
105_S109_E1.6061.00
250_V261_G1.5961.00
100_M130_L1.5911.00
127_N261_G1.5801.00
144_F230_V1.5771.00
42_T46_A1.5621.00
4_V21_L1.5561.00
283_A287_D1.5291.00
145_G263_G1.5271.00
181_V285_E1.5231.00
87_Q95_D1.5221.00
40_E43_I1.5141.00
235_E239_N1.5081.00
6_I18_G1.4991.00
98_P101_E1.4901.00
50_N159_Q1.4781.00
288_L291_E1.4591.00
271_D274_E1.4521.00
66_Y110_H1.4471.00
128_R132_E1.4461.00
143_G248_S1.4401.00
17_A230_V1.4371.00
172_I182_P1.4231.00
158_G168_V1.4131.00
50_N155_Y1.3801.00
67_A70_A1.3761.00
83_R212_A1.3751.00
147_R229_G1.3521.00
291_E294_E1.3491.00
66_Y109_E1.3441.00
44_G218_K1.3421.00
157_L168_V1.3251.00
128_R300_Y1.3121.00
202_E206_A1.3101.00
69_T72_S1.3061.00
257_L293_A1.2931.00
19_Y227_A1.2891.00
284_D288_L1.2851.00
182_P205_L1.2821.00
88_S91_D1.2811.00
169_D188_R1.2761.00
39_E43_I1.2711.00
147_R245_L1.2631.00
52_G228_T1.2611.00
196_F200_E1.2581.00
179_A295_K1.2571.00
287_D290_D1.2541.00
164_P167_N1.2531.00
62_V68_D1.2531.00
210_E213_M1.2521.00
129_H133_S1.2481.00
107_L136_R1.2381.00
77_I118_T1.2361.00
131_Y250_V1.2221.00
115_V140_S1.2161.00
185_S285_E1.2101.00
251_L254_E1.2071.00
206_A209_Q1.2041.00
10_A32_F1.1961.00
81_I84_Q1.1951.00
74_V117_V1.1941.00
36_P40_E1.1711.00
39_E65_D1.1711.00
100_M104_G1.1691.00
75_V107_L1.1631.00
73_D112_D1.1611.00
107_L116_T1.1541.00
167_N190_N1.1511.00
297_S301_D1.1451.00
240_D270_S1.1421.00
160_R203_E1.1391.00
214_N218_K1.1371.00
113_D137_D1.1351.00
17_A234_V1.1331.00
3_K71_G1.1321.00
214_N217_E1.1321.00
123_V143_G1.1271.00
142_V237_I1.1221.00
111_N114_F1.1201.00
31_V68_D1.1201.00
21_L30_L1.1171.00
51_H211_S1.1171.00
19_Y23_L1.1131.00
40_E217_E1.1121.00
108_A134_G1.1121.00
17_A76_V1.1101.00
185_S282_T1.1091.00
102_D105_S1.1081.00
290_D293_A1.1001.00
36_P41_E1.0971.00
200_E203_E1.0891.00
63_Q68_D1.0881.00
118_T130_L1.0871.00
21_L26_V1.0831.00
121_N176_H1.0781.00
58_N61_V1.0761.00
298_A301_D1.0751.00
92_L96_N1.0721.00
249_M273_V1.0681.00
75_V114_F1.0661.00
252_D258_D1.0631.00
247_G266_V1.0631.00
95_D98_P1.0590.99
40_E218_K1.0580.99
232_H243_E1.0550.99
128_R131_Y1.0460.99
77_I130_L1.0450.99
139_H271_D1.0440.99
123_V127_N1.0440.99
291_E295_K1.0430.99
89_R121_N1.0400.99
65_D68_D1.0390.99
253_G256_G1.0360.99
267_K274_E1.0350.99
101_E133_S1.0230.99
89_R176_H1.0150.99
17_A227_A1.0110.99
179_A292_A1.0100.99
94_G98_P1.0070.99
244_V267_K1.0010.99
83_R216_I0.9990.99
12_T223_Q0.9980.99
26_V239_N0.9810.99
15_A45_Q0.9730.99
155_Y159_Q0.9630.99
33_V66_Y0.9560.99
267_K277_V0.9560.99
76_V117_V0.9540.99
284_D287_D0.9520.99
91_D94_G0.9480.99
154_R169_D0.9460.99
81_I99_I0.9430.99
117_V233_M0.9430.99
73_D113_D0.9390.99
87_Q91_D0.9300.99
289_L293_A0.9300.99
281_L289_L0.9300.99
42_T64_G0.9240.99
166_Q241_T0.9230.99
15_A227_A0.9220.99
104_G116_T0.9220.99
31_V72_S0.9180.99
240_D243_E0.9170.99
64_G68_D0.9160.99
18_G32_F0.9110.98
24_R231_A0.9100.98
179_A291_E0.9090.98
29_E62_V0.9060.98
227_A231_A0.9050.98
74_V238_L0.9020.98
210_E217_E0.9000.98
158_G165_V0.8990.98
183_V281_L0.8970.98
29_E60_D0.8910.98
71_G112_D0.8900.98
153_F170_A0.8850.98
254_E277_V0.8840.98
212_A216_I0.8830.98
131_Y138_R0.8800.98
70_A110_H0.8770.98
41_E45_Q0.8700.98
26_V231_A0.8680.98
77_I100_M0.8580.98
138_R250_V0.8570.98
150_S154_R0.8560.98
75_V111_N0.8540.98
235_E241_T0.8500.98
127_N141_V0.8470.98
72_S75_V0.8470.98
156_V207_D0.8420.97
233_M247_G0.8390.97
104_G133_S0.8380.97
127_N143_G0.8360.97
125_L299_Q0.8360.97
83_R213_M0.8320.97
188_R191_G0.8300.97
137_D140_S0.8290.97
230_V234_V0.8230.97
117_V234_V0.8190.97
85_P217_E0.8180.97
67_A110_H0.8140.97
156_V159_Q0.8080.97
295_K299_Q0.8070.97
136_R141_V0.8070.97
66_Y103_I0.8060.97
153_F182_P0.8030.97
264_V276_V0.7980.96
173_L181_V0.7900.96
12_T15_A0.7900.96
10_A45_Q0.7880.96
100_M118_T0.7860.96
294_E297_S0.7860.96
83_R87_Q0.7830.96
71_G113_D0.7820.96
77_I103_I0.7810.96
141_V261_G0.7780.96
123_V145_G0.7760.96
163_V189_V0.7740.96
294_E301_D0.7660.96
83_R92_L0.7640.95
17_A21_L0.7630.95
128_R259_D0.7620.95
100_M126_L0.7600.95
92_L216_I0.7590.95
31_V64_G0.7540.95
43_I218_K0.7500.95
157_L161_F0.7470.95
99_I120_S0.7460.95
269_G272_G0.7440.95
20_N227_A0.7440.95
205_L209_Q0.7440.95
39_E63_Q0.7440.95
184_F208_L0.7440.95
100_M133_S0.7400.95
114_F136_R0.7400.95
163_V168_V0.7380.95
39_E64_G0.7350.94
255_Y259_D0.7350.94
33_V69_T0.7320.94
117_V237_I0.7290.94
246_P263_G0.7270.94
147_R233_M0.7240.94
274_E277_V0.7220.94
6_I17_A0.7200.94
79_A99_I0.7200.94
16_A20_N0.7200.94
89_R92_L0.7200.94
20_N23_L0.7180.94
178_D213_M0.7170.94
199_D203_E0.7160.94
199_D202_E0.7140.94
139_H272_G0.7120.93
153_F187_V0.7110.93
233_M245_L0.7110.93
66_Y70_A0.7080.93
43_I64_G0.7060.93
17_A78_T0.7030.93
213_M217_E0.7020.93
115_V142_V0.6970.93
131_Y134_G0.6970.93
172_I205_L0.6960.93
76_V230_V0.6950.93
104_G108_A0.6950.93
21_L231_A0.6920.92
161_F168_V0.6890.92
72_S111_N0.6890.92
247_G268_L0.6860.92
242_G267_K0.6830.92
90_T125_L0.6820.92
12_T221_A0.6810.92
7_V103_I0.6800.92
215_V225_G0.6780.92
108_A114_F0.6770.92
16_A224_W0.6770.92
8_G11_G0.6760.92
252_D277_V0.6760.92
202_E284_D0.6750.91
58_N164_P0.6700.91
16_A223_Q0.6680.91
293_A297_S0.6660.91
161_F196_F0.6650.91
203_E206_A0.6600.91
96_N126_L0.6600.91
18_G49_A0.6600.91
88_S178_D0.6580.90
240_D269_G0.6540.90
219_K222_T0.6520.90
21_L234_V0.6520.90
45_Q227_A0.6520.90
119_T144_F0.6510.90
257_L286_R0.6470.90
194_P197_S0.6430.89
98_P102_D0.6430.89
46_A50_N0.6400.89
210_E214_N0.6390.89
260_V293_A0.6370.89
154_R232_H0.6350.89
253_G278_E0.6340.89
181_V292_A0.6320.89
36_P42_T0.6320.89
21_L27_V0.6320.89
45_Q219_K0.6310.89
78_T119_T0.6300.88
196_F201_R0.6290.88
108_A116_T0.6290.88
84_Q95_D0.6280.88
216_I221_A0.6230.88
196_F199_D0.6220.88
260_V297_S0.6200.88
89_R149_D0.6190.88
104_G126_L0.6170.87
5_S31_V0.6170.87
225_G228_T0.6160.87
46_A61_V0.6160.87
130_L134_G0.6140.87
281_L290_D0.6130.87
51_H155_Y0.6120.87
149_D152_R0.6060.86
31_V62_V0.6050.86
236_A243_E0.6040.86
275_E278_E0.6020.86
90_T299_Q0.6010.86
20_N231_A0.5990.86
257_L297_S0.5990.86
33_V103_I0.5980.86
15_A224_W0.5980.86
158_G163_V0.5980.86
90_T295_K0.5950.85
56_D59_T0.5930.85
5_S111_N0.5920.85
10_A30_L0.5910.85
80_G120_S0.5910.85
272_G277_V0.5900.85
272_G275_E0.5900.85
186_K279_W0.5890.85
130_L141_V0.5830.84
60_D159_Q0.5820.84
19_Y224_W0.5820.84
236_A245_L0.5820.84
62_V67_A0.5810.84
252_D259_D0.5810.84
173_L279_W0.5810.84
243_E267_K0.5750.83
195_A198_A0.5740.83
66_Y106_S0.5740.83
236_A246_P0.5740.83
27_V30_L0.5730.83
212_A222_T0.5720.83
107_L110_H0.5710.83
176_H212_A0.5650.82
181_V288_L0.5640.82
113_D140_S0.5630.82
138_R261_G0.5610.82
59_T62_V0.5610.82
132_E250_V0.5610.82
123_V144_F0.5600.82
181_V281_L0.5590.82
254_E264_V0.5580.82
232_H241_T0.5550.81
69_T108_A0.5550.81
140_S271_D0.5530.81
183_V186_K0.5530.81
22_A49_A0.5500.81
4_V30_L0.5500.81
77_I104_G0.5490.81
126_L130_L0.5460.80
85_P220_G0.5450.80
273_V276_V0.5440.80
22_A30_L0.5440.80
254_E278_E0.5430.80
97_A133_S0.5430.80
280_E286_R0.5420.80
157_L170_A0.5400.80
269_G273_V0.5400.80
181_V205_L0.5380.79
257_L262_L0.5380.79
270_S274_E0.5380.79
121_N222_T0.5370.79
173_L281_L0.5360.79
281_L285_E0.5360.79
249_M252_D0.5350.79
249_M263_G0.5320.79
44_G92_L0.5310.78
205_L288_L0.5310.78
36_P39_E0.5290.78
109_E133_S0.5280.78
144_F263_G0.5280.78
6_I30_L0.5280.78
156_V160_R0.5250.78
13_V119_T0.5240.77
106_S110_H0.5240.77
71_G111_N0.5240.77
212_A221_A0.5230.77
157_L196_F0.5220.77
80_G83_R0.5220.77
154_R170_A0.5180.77
254_E275_E0.5170.77
139_H274_E0.5150.76
103_I107_L0.5150.76
159_Q207_D0.5140.76
16_A19_Y0.5130.76
74_V115_V0.5130.76
236_A241_T0.5130.76
4_V74_V0.5120.76
87_Q92_L0.5090.75
7_V69_T0.5090.75
26_V235_E0.5080.75
11_G149_D0.5050.75
144_F147_R0.5030.75
151_A225_G0.5030.75
17_A231_A0.5000.74
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2d4aB 4 0.9803 100 0.221 Contact Map
4cl3A 4 0.9868 100 0.229 Contact Map
3nepX 4 0.9638 100 0.231 Contact Map
1a5zA 3 0.9868 100 0.234 Contact Map
1lldA 3 0.9901 100 0.236 Contact Map
4plzA 4 0.9638 100 0.239 Contact Map
1guzA 4 0.9836 100 0.243 Contact Map
2v6bA 4 0.9013 100 0.25 Contact Map
2hjrA 4 0.9836 100 0.254 Contact Map
4aj2A 4 0.9901 100 0.256 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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