GREMLIN Database
COFE - Coenzyme F420:L-glutamate ligase
UniProt: Q9HME1 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 253 (225)
Sequences: 691 (441)
Seq/√Len: 29.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
81_Q85_E4.7001.00
99_T110_A4.1911.00
22_V215_V4.0271.00
35_C149_T4.0151.00
145_G170_L3.5881.00
45_E132_S3.5561.00
47_R124_L3.4851.00
18_L40_V3.1581.00
5_A213_T2.9251.00
12_I21_L2.7191.00
198_L221_F2.7111.00
167_W172_A2.6861.00
11_E182_R2.6221.00
82_A112_I2.5660.99
32_D219_R2.5020.99
137_Q168_A2.4600.99
14_A48_T2.4030.99
23_A140_L2.3840.99
12_I44_A2.3610.99
19_A139_G2.1860.98
88_T100_E2.1710.98
18_L136_I2.1520.98
162_G203_N2.1230.98
193_A232_L2.0940.98
9_L12_I2.0930.98
17_D139_G2.0560.97
131_E135_R2.0320.97
36_V136_I2.0290.97
203_N208_E2.0080.97
3_A216_V2.0000.97
30_D143_D1.9760.97
50_A53_E1.9010.96
151_T160_Q1.8440.95
152_S163_V1.7990.94
34_V217_V1.7740.94
100_E242_R1.7620.94
158_H238_G1.6750.92
134_A138_A1.6640.91
107_G152_S1.6280.91
48_T123_L1.5190.87
235_A238_G1.5140.87
137_Q169_G1.5140.87
9_L22_V1.5080.87
128_R131_E1.4960.86
6_V12_I1.4490.85
175_D191_V1.4490.85
34_V198_L1.4430.84
5_A215_V1.4410.84
205_V216_V1.4410.84
108_V241_V1.4400.84
162_G208_E1.4170.83
4_F219_R1.3980.82
198_L218_V1.3950.82
105_H110_A1.3610.81
21_L44_A1.3600.80
7_D213_T1.3460.80
151_T197_E1.3200.78
140_L144_V1.3150.78
160_Q189_V1.2900.77
147_V198_L1.2890.77
13_D16_D1.2810.76
9_L21_L1.2780.76
186_E198_L1.2730.76
228_G231_Q1.2700.76
162_G182_R1.2670.75
81_Q84_I1.2640.75
82_A122_L1.2600.75
18_L41_V1.2480.74
41_V148_V1.2460.74
34_V140_L1.2360.74
147_V221_F1.2080.72
61_E65_A1.1910.71
28_L34_V1.1860.70
130_A241_V1.1700.69
51_L78_R1.1520.68
38_S123_L1.1470.68
130_A234_R1.1420.67
92_M152_S1.1410.67
4_F217_V1.1340.67
162_G206_S1.1340.67
37_A216_V1.1340.67
172_A231_Q1.1310.67
77_P216_V1.1200.66
101_T105_H1.1190.66
171_P194_V1.1150.66
130_A166_G1.1140.66
60_A64_A1.1130.65
101_T172_A1.1120.65
35_C218_V1.1040.65
117_T123_L1.1030.65
182_R208_E1.0790.63
98_L108_V1.0570.61
36_V135_R1.0540.61
112_I122_L1.0390.60
47_R127_K1.0390.60
84_I91_I1.0360.60
5_A103_C1.0300.59
47_R132_S1.0210.59
131_E141_A1.0200.59
153_G234_R1.0180.58
6_V213_T1.0170.58
160_Q176_W1.0140.58
34_V202_A1.0120.58
30_D142_A0.9980.57
234_R241_V0.9980.57
137_Q145_G0.9950.57
3_A201_T0.9850.56
66_R146_V0.9850.56
114_R122_L0.9830.56
39_T156_F0.9800.56
39_T124_L0.9640.54
176_W179_E0.9630.54
197_E208_E0.9610.54
60_A81_Q0.9600.54
43_K197_E0.9490.53
39_T96_F0.9490.53
40_V214_P0.9430.53
112_I171_P0.9320.52
160_Q208_E0.9290.52
43_K154_R0.9260.51
187_L190_T0.9250.51
236_V242_R0.9140.50
135_R139_G0.9020.49
146_V217_V0.8990.49
167_W170_L0.8960.49
4_F29_T0.8960.49
38_S117_T0.8950.49
145_G223_F0.8930.49
156_F241_V0.8910.49
166_G234_R0.8900.48
35_C198_L0.8850.48
89_E236_V0.8840.48
170_L221_F0.8840.48
88_T102_T0.8800.48
167_W195_V0.8780.48
141_A144_V0.8720.47
208_E211_D0.8720.47
38_S109_N0.8680.47
108_V156_F0.8680.47
111_G169_G0.8630.46
180_T187_L0.8610.46
42_S201_T0.8590.46
153_G166_G0.8560.46
5_A205_V0.8510.45
41_V213_T0.8440.45
63_I136_I0.8360.44
157_R168_A0.8330.44
63_I170_L0.8120.42
20_A24_E0.8110.42
17_D20_A0.8070.42
11_E183_D0.8060.42
18_L44_A0.8030.42
130_A153_G0.8010.42
105_H152_S0.7990.41
177_R191_V0.7990.41
24_E33_V0.7990.41
49_A147_V0.7980.41
88_T99_T0.7960.41
60_A84_I0.7910.41
176_W209_G0.7860.40
26_A144_V0.7830.40
23_A139_G0.7800.40
79_F122_L0.7800.40
153_G156_F0.7790.40
180_T186_E0.7750.40
3_A168_A0.7720.39
152_S208_E0.7720.39
11_E162_G0.7700.39
151_T208_E0.7700.39
200_A203_N0.7610.39
173_A232_L0.7600.38
158_H231_Q0.7580.38
9_L44_A0.7580.38
96_F108_V0.7560.38
174_R240_F0.7480.38
138_A227_D0.7450.37
156_F234_R0.7440.37
18_L34_V0.7390.37
52_A120_A0.7370.37
177_R192_E0.7360.37
89_E100_E0.7300.36
16_D21_L0.7280.36
96_F156_F0.7270.36
134_A228_G0.7260.36
37_A60_A0.7240.36
90_V99_T0.7220.36
24_E29_T0.7220.36
30_D134_A0.7190.35
140_L202_A0.7190.35
144_V185_H0.7170.35
239_D242_R0.7140.35
42_S123_L0.7140.35
20_A50_A0.7130.35
26_A140_L0.7110.35
118_G183_D0.7090.35
22_V34_V0.7070.35
45_E240_F0.7060.35
203_N206_S0.7060.35
81_Q90_V0.7060.35
108_V234_R0.7060.35
206_S216_V0.7060.35
125_L166_G0.7050.34
89_E189_V0.7030.34
165_L240_F0.7020.34
100_E155_P0.7020.34
130_A220_E0.7010.34
38_S124_L0.7000.34
98_L105_H0.6910.33
151_T234_R0.6900.33
173_A191_V0.6870.33
172_A229_S0.6850.33
163_V167_W0.6820.33
77_P84_I0.6810.33
34_V164_A0.6810.33
38_S162_G0.6800.33
29_T219_R0.6790.33
35_C143_D0.6770.32
92_M241_V0.6750.32
166_G241_V0.6740.32
100_E106_V0.6720.32
174_R186_E0.6660.32
53_E135_R0.6640.32
112_I124_L0.6630.32
28_L215_V0.6600.31
92_M97_L0.6590.31
154_R234_R0.6590.31
14_A179_E0.6580.31
22_V180_T0.6530.31
202_A206_S0.6530.31
108_V130_A0.6510.31
51_L112_I0.6490.31
29_T118_G0.6480.31
53_E117_T0.6460.30
45_E135_R0.6440.30
230_E233_F0.6420.30
50_A91_I0.6410.30
33_V122_L0.6400.30
8_G15_G0.6330.30
10_P51_L0.6320.29
37_A55_T0.6310.29
130_A167_W0.6300.29
8_G25_R0.6290.29
106_V169_G0.6260.29
34_V240_F0.6240.29
105_H208_E0.6170.28
149_T199_A0.6170.28
76_D80_A0.6130.28
165_L228_G0.6110.28
23_A26_A0.6100.28
152_S193_A0.6100.28
59_R62_E0.6080.28
203_N241_V0.6080.28
116_N181_D0.6050.28
5_A136_I0.6050.28
79_F91_I0.6040.28
14_A28_L0.5990.27
6_V9_L0.5990.27
134_A168_A0.5990.27
28_L144_V0.5980.27
173_A189_V0.5970.27
106_V154_R0.5960.27
33_V137_Q0.5950.27
62_E65_A0.5930.27
93_D112_I0.5910.27
139_G142_A0.5880.27
38_S90_V0.5880.27
81_Q91_I0.5870.27
21_L175_D0.5850.26
234_R239_D0.5780.26
96_F240_F0.5760.26
100_E236_V0.5760.26
39_T98_L0.5730.26
197_E239_D0.5730.26
62_E66_R0.5730.26
116_N190_T0.5710.26
33_V145_G0.5690.26
46_G120_A0.5660.25
197_E203_N0.5650.25
80_A97_L0.5650.25
34_V213_T0.5640.25
6_V27_D0.5640.25
79_F83_V0.5630.25
115_S181_D0.5620.25
16_D148_V0.5620.25
190_T208_E0.5600.25
153_G241_V0.5600.25
11_E147_V0.5600.25
45_E215_V0.5600.25
43_K156_F0.5590.25
92_M108_V0.5590.25
30_D144_V0.5580.25
7_D10_P0.5530.25
98_L101_T0.5510.24
155_P163_V0.5500.24
79_F82_A0.5490.24
92_M156_F0.5450.24
43_K106_V0.5430.24
182_R203_N0.5420.24
30_D190_T0.5400.24
50_A121_E0.5390.24
101_T203_N0.5390.24
34_V180_T0.5370.24
173_A202_A0.5370.24
52_A225_D0.5370.24
40_V240_F0.5360.24
193_A226_H0.5350.24
104_G154_R0.5340.23
4_F33_V0.5330.23
122_L142_A0.5330.23
129_P229_S0.5310.23
133_A148_V0.5300.23
98_L215_V0.5280.23
60_A97_L0.5270.23
155_P201_T0.5260.23
98_L192_E0.5260.23
36_V217_V0.5250.23
39_T108_V0.5250.23
106_V153_G0.5240.23
7_D20_A0.5230.23
17_D136_I0.5190.23
81_Q109_N0.5180.23
154_R239_D0.5180.23
4_F7_D0.5180.23
33_V66_R0.5170.23
39_T152_S0.5150.22
35_C202_A0.5110.22
156_F239_D0.5110.22
167_W229_S0.5090.22
176_W211_D0.5080.22
84_I95_P0.5080.22
108_V154_R0.5070.22
183_D209_G0.5060.22
50_A120_A0.5060.22
19_A180_T0.5030.22
225_D228_G0.5030.22
105_H229_S0.5020.22
115_S187_L0.5010.22
192_E203_N0.5000.22
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2phnA 2 0.9605 100 0.22 Contact Map
4ig1A 1 0.8419 22.3 0.969 Contact Map
3g98A 1 0.3518 18.8 0.97 Contact Map
2o34A 1 0.8221 11.9 0.972 Contact Map
4ob9A 2 0.2885 11.1 0.973 Contact Map
1um0A 4 0.3953 10.6 0.973 Contact Map
1xe1A 2 0.1502 9.9 0.973 Contact Map
1r8oA 1 0.3043 9.4 0.974 Contact Map
3eiqA 1 0.5375 8.5 0.974 Contact Map
1sq1A 4 0.2332 8.1 0.974 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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