GREMLIN Database
TBPB - TATA-box-binding protein B
UniProt: Q48325 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
Length: 186 (174)
Sequences: 532 (341)
Seq/√Len: 25.8

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
23_L28_L5.3061.00
54_S69_A4.2631.00
35_A45_V2.9821.00
142_L145_P2.9131.00
59_F67_T2.8221.00
42_F57_L2.7871.00
135_F152_F2.5560.99
15_A75_V2.5060.99
80_G84_G2.4300.99
42_F59_F2.4010.99
84_G87_R2.3650.98
54_S78_A2.3270.98
44_G154_S2.2910.98
150_L158_V2.2400.98
67_T160_T2.2350.98
12_N103_N2.1190.97
147_V171_A2.0460.96
105_V158_V1.9330.94
46_V59_F1.9140.94
36_E48_R1.8970.94
26_D30_T1.8780.93
54_S82_V1.8540.93
46_V57_L1.8450.93
13_V71_S1.8200.92
124_G127_Q1.8190.92
18_D98_P1.8170.92
149_V175_V1.7680.91
142_L175_V1.7450.91
13_V75_V1.7440.91
149_V157_L1.6720.89
159_I168_A1.6640.88
132_P155_G1.6170.87
135_F150_L1.6040.86
19_L86_I1.5960.86
33_P40_E1.5790.86
22_E60_R1.5550.85
142_L174_H1.5450.84
102_Q105_V1.5070.83
136_P152_F1.4460.80
119_I149_V1.3840.77
57_L65_V1.3680.76
47_Y58_I1.3190.73
17_S64_V1.3030.72
154_S179_L1.2800.71
8_I172_L1.2800.71
56_T82_V1.2770.71
144_D170_Q1.2630.70
47_Y85_D1.2620.70
44_G57_L1.2410.69
124_G129_E1.2380.68
91_I95_S1.2340.68
110_L159_I1.1920.65
13_V144_D1.1870.65
54_S75_V1.1840.65
103_N160_T1.1810.65
65_V107_S1.1790.65
42_F103_N1.1640.64
45_V168_A1.1480.63
21_Q91_I1.1290.61
124_G128_I1.1190.60
19_L83_F1.1140.60
10_I20_G1.1100.60
120_A138_L1.1070.60
49_L107_S1.1050.59
139_V150_L1.1030.59
124_G140_Y1.0850.58
71_S78_A1.0840.58
61_S131_E1.0830.58
12_N160_T1.0810.58
15_A66_C1.0680.57
50_Q178_R1.0650.57
31_D89_L1.0610.56
44_G59_F1.0580.56
20_G71_S1.0520.56
173_A181_E1.0420.55
42_F46_V1.0410.55
52_P145_P1.0350.54
162_G171_A1.0300.54
101_V151_L1.0290.54
46_V55_A1.0240.54
11_E107_S1.0230.54
59_F65_V1.0220.53
41_D146_D1.0180.53
55_A59_F1.0160.53
137_G154_S1.0130.53
27_Q180_T1.0050.52
117_N121_I1.0040.52
137_G162_G0.9960.52
109_S156_K0.9930.51
67_T102_Q0.9920.51
66_C75_V0.9900.51
40_E159_I0.9880.51
124_G178_R0.9860.51
148_V160_T0.9720.50
93_V151_L0.9690.50
10_I49_L0.9680.50
63_K103_N0.9650.49
77_E81_I0.9590.49
115_N153_G0.9560.49
104_I161_G0.9550.49
131_E137_G0.9550.49
45_V126_E0.9540.48
105_V150_L0.9350.47
66_C175_V0.9340.47
170_Q173_A0.9260.46
24_A27_Q0.9250.46
68_G132_P0.9170.46
114_L151_L0.9140.46
35_A181_E0.9080.45
21_Q95_S0.9060.45
49_L59_F0.8980.44
15_A43_P0.8930.44
59_F135_F0.8930.44
23_L37_Y0.8850.43
58_I136_P0.8800.43
41_D45_V0.8800.43
24_A114_L0.8790.43
57_L150_L0.8730.43
16_S46_V0.8690.42
27_Q31_D0.8620.42
20_G179_L0.8560.41
55_A79_L0.8500.41
121_I126_E0.8470.41
84_G178_R0.8400.40
150_L156_K0.8370.40
17_S111_E0.8240.39
74_D164_N0.8180.39
75_V78_A0.8170.39
53_K154_S0.8110.38
119_I138_L0.8090.38
24_A113_S0.8070.38
81_I177_D0.8060.38
49_L78_A0.8060.38
66_C69_A0.7970.37
10_I107_S0.7960.37
51_E92_D0.7950.37
22_E118_A0.7950.37
65_V78_A0.7900.37
106_S110_L0.7890.37
29_S86_I0.7880.37
28_L36_E0.7840.36
40_E115_N0.7820.36
144_D174_H0.7800.36
9_H70_K0.7710.35
127_Q140_Y0.7690.35
45_V106_S0.7680.35
120_A126_E0.7670.35
62_G68_G0.7670.35
21_Q28_L0.7590.35
29_S35_A0.7560.34
10_I147_V0.7540.34
59_F64_V0.7510.34
31_D38_N0.7480.34
120_A171_A0.7470.34
8_I108_A0.7400.33
68_G161_G0.7340.33
108_A168_A0.7330.33
13_V101_V0.7320.33
9_H21_Q0.7300.33
162_G165_P0.7280.33
53_K146_D0.7270.33
169_E173_A0.7260.32
71_S111_E0.7230.32
48_R52_P0.7220.32
48_R90_G0.7210.32
9_H140_Y0.7140.32
17_S128_I0.7090.31
38_N107_S0.7050.31
59_F63_K0.7040.31
15_A116_L0.6980.31
14_V42_F0.6960.31
141_R154_S0.6810.30
125_L129_E0.6710.29
47_Y55_A0.6710.29
57_L135_F0.6680.29
157_L177_D0.6630.29
29_S60_R0.6570.28
28_L107_S0.6570.28
102_Q136_P0.6560.28
102_Q163_Q0.6550.28
105_V160_T0.6530.28
82_V107_S0.6510.28
33_P127_Q0.6500.28
27_Q122_G0.6450.27
159_I172_L0.6430.27
89_L165_P0.6400.27
51_E101_V0.6390.27
53_K145_P0.6350.27
104_I164_N0.6340.27
48_R102_Q0.6330.27
11_E119_I0.6270.26
85_D88_E0.6250.26
128_I182_L0.6170.26
61_S146_D0.6160.26
58_I106_S0.6140.26
129_E134_Q0.6140.26
16_S19_L0.6110.26
42_F55_A0.6110.26
133_E155_G0.6080.25
118_A121_I0.6070.25
19_L176_Q0.6040.25
103_N158_V0.6040.25
147_V162_G0.6020.25
43_P110_L0.6020.25
35_A175_V0.6020.25
38_N113_S0.5970.25
21_Q89_L0.5970.25
64_V114_L0.5950.25
32_L118_A0.5940.25
13_V157_L0.5900.24
61_S74_D0.5900.24
79_L108_A0.5890.24
47_Y65_V0.5880.24
39_P119_I0.5870.24
99_I113_S0.5810.24
8_I101_V0.5810.24
16_S55_A0.5740.23
61_S147_V0.5730.23
137_G160_T0.5730.23
126_E143_D0.5710.23
7_T169_E0.5700.23
156_K161_G0.5690.23
95_S104_I0.5690.23
27_Q181_E0.5670.23
43_P115_N0.5660.23
83_F88_E0.5650.23
70_K159_I0.5640.23
156_K163_Q0.5630.23
111_E151_L0.5590.23
35_A172_L0.5580.23
136_P140_Y0.5520.22
79_L97_P0.5440.22
25_L151_L0.5440.22
127_Q153_G0.5410.22
102_Q158_V0.5410.22
137_G155_G0.5380.22
28_L61_S0.5380.22
8_I75_V0.5330.21
131_E141_R0.5300.21
34_G48_R0.5300.21
148_V153_G0.5290.21
28_L47_Y0.5260.21
135_F158_V0.5250.21
150_L160_T0.5240.21
26_D104_I0.5230.21
12_N130_Y0.5220.21
122_G168_A0.5220.21
35_A47_Y0.5220.21
13_V106_S0.5210.21
38_N56_T0.5180.21
157_L176_Q0.5150.21
111_E177_D0.5110.20
16_S102_Q0.5100.20
83_F87_R0.5080.20
8_I48_R0.5060.20
122_G182_L0.5040.20
164_N168_A0.5040.20
73_D174_H0.5040.20
14_V133_E0.5030.20
152_F158_V0.5010.20
178_R182_L0.5000.20
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1rm1A 1 0.9677 100 0.065 Contact Map
4b0aA 1 0.9624 100 0.069 Contact Map
1ytbA 1 0.9677 100 0.074 Contact Map
3eikA 2 0.957 100 0.078 Contact Map
1mp9A 2 0.9892 100 0.114 Contact Map
1aisA 1 0.9677 100 0.115 Contact Map
2z8uA 2 0.9462 100 0.117 Contact Map
2d0bA 1 0.4247 87.1 0.921 Contact Map
3vn5A 1 0.3871 74.4 0.931 Contact Map
4py5A 1 0.3817 69.1 0.934 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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