GREMLIN Database
CAS5 - CRISPR system Cascade subunit CasD
UniProt: Q46898 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13116
Length: 224 (196)
Sequences: 666 (561)
Seq/√Len: 40.1

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
40_L133_L7.7411.00
46_I56_A6.6051.00
62_Q121_W3.9571.00
111_L116_F3.7951.00
36_L63_F3.7561.00
166_D169_K3.5921.00
18_Q28_G3.1161.00
50_D53_S2.8301.00
43_C144_P2.7911.00
47_Q50_D2.6791.00
13_M151_C2.5511.00
64_A167_P2.3681.00
162_C174_Y2.3671.00
90_G93_E2.2271.00
36_L120_L2.1690.99
6_I119_A2.1460.99
131_S161_T2.1270.99
134_E138_L2.0700.99
18_Q32_T2.0270.99
201_M209_A1.9080.98
47_Q142_Y1.8250.98
165_S170_A1.8150.98
28_G32_T1.7890.98
14_Q116_F1.7700.97
42_A142_Y1.7600.97
198_D211_R1.7170.97
203_T206_R1.7050.97
50_D54_L1.6390.96
40_L120_L1.6200.96
30_F213_W1.5980.95
10_A154_T1.5930.95
20_T26_P1.5900.95
130_I161_T1.5860.95
141_R161_T1.5490.95
197_R210_S1.5490.95
37_L63_F1.5400.94
85_Y89_L1.5240.94
84_D87_T1.4920.93
10_A115_S1.4760.93
124_P164_A1.4760.93
125_H131_S1.4700.93
11_G146_L1.4670.93
135_K139_K1.4630.93
85_Y88_V1.4600.93
46_I54_L1.4400.92
68_D117_T1.3650.90
81_G84_D1.3510.89
146_L153_L1.3450.89
119_A167_P1.3420.89
7_L159_L1.3410.89
201_M207_Q1.3410.89
88_V104_I1.3340.89
66_R175_E1.3260.88
16_W35_G1.3220.88
162_C170_A1.3000.87
144_P157_L1.2880.87
69_E178_G1.2560.86
63_F120_L1.2390.85
44_L57_L1.2310.84
6_I160_G1.2010.83
32_T196_A1.2010.83
149_R159_L1.2000.83
8_R155_H1.1950.83
15_A27_T1.1830.82
85_Y105_Q1.1800.82
14_Q114_A1.1720.81
7_L160_G1.1670.81
121_W167_P1.1640.81
141_R144_P1.1510.80
168_Q172_L1.1490.80
123_T129_V1.1480.80
117_T174_Y1.1460.80
5_L161_T1.1410.79
165_S169_K1.1390.79
6_I117_T1.1370.79
169_K173_N1.1360.79
14_Q35_G1.1350.79
43_C137_V1.1350.79
20_T112_C1.1340.79
23_G103_T1.1290.79
139_K158_F1.1140.78
119_A171_L1.0860.76
203_T208_F1.0800.76
22_E106_T1.0770.75
170_A207_Q1.0730.75
32_T111_L1.0720.75
164_A170_A1.0600.74
13_M153_L1.0580.74
123_T169_K1.0430.73
167_P174_Y1.0420.73
29_R208_F1.0250.72
66_R116_F1.0110.71
44_L136_A1.0030.70
15_A119_A0.9980.70
64_A117_T0.9900.69
24_T109_E0.9870.69
190_H193_K0.9840.69
9_L36_L0.9730.68
13_M185_E0.9620.67
14_Q111_L0.9620.67
134_E158_F0.9530.66
93_E106_T0.9480.66
134_E161_T0.9450.65
8_R177_V0.9390.65
16_W146_L0.9360.65
84_D88_V0.9340.64
196_A213_W0.9290.64
9_L146_L0.9290.64
157_L167_P0.9250.64
62_Q132_E0.9250.64
149_R152_P0.9210.63
66_R206_R0.9200.63
21_F35_G0.9180.63
117_T151_C0.9030.62
199_E213_W0.8990.61
188_T191_H0.8960.61
91_A94_D0.8870.60
24_T107_W0.8860.60
18_Q195_T0.8810.60
33_R63_F0.8810.60
7_L158_F0.8750.59
85_Y103_T0.8730.59
171_L175_E0.8710.59
125_H173_N0.8680.59
82_L91_A0.8630.58
8_R115_S0.8570.58
81_G145_Y0.8320.55
25_R152_P0.8300.55
13_M82_L0.8280.55
80_T84_D0.8200.54
10_A22_E0.8190.54
120_L130_I0.8140.54
49_D56_A0.8130.54
44_L132_E0.8100.53
86_H104_I0.8090.53
84_D89_L0.8020.53
117_T177_V0.7930.52
81_G85_Y0.7880.51
46_I50_D0.7840.51
43_C143_T0.7810.51
15_A29_R0.7800.51
11_G16_W0.7790.51
62_Q167_P0.7780.51
135_K207_Q0.7640.49
33_R58_S0.7630.49
66_R117_T0.7580.49
8_R100_S0.7510.48
41_G68_D0.7480.48
13_M112_C0.7430.47
33_R36_L0.7430.47
167_P181_I0.7410.47
104_I208_F0.7400.47
145_Y195_T0.7400.47
62_Q154_T0.7390.47
48_R206_R0.7290.46
42_A145_Y0.7270.46
81_G144_P0.7250.46
46_I53_S0.7210.45
14_Q146_L0.7110.44
21_F213_W0.7110.44
21_F86_H0.7110.44
81_G90_G0.7070.44
49_D131_S0.7030.44
41_G142_Y0.7020.44
27_T109_E0.7010.44
36_L118_V0.6990.43
119_A122_L0.6970.43
23_G106_T0.6940.43
193_K215_V0.6930.43
8_R146_L0.6890.42
58_S199_E0.6860.42
193_K212_E0.6850.42
111_L206_R0.6780.41
68_D116_F0.6780.41
6_I174_Y0.6750.41
41_G149_R0.6750.41
123_T197_R0.6710.41
81_G103_T0.6700.41
25_R113_D0.6660.40
6_I10_A0.6620.40
55_Q60_S0.6620.40
124_P130_I0.6610.40
14_Q206_R0.6610.40
44_L133_L0.6500.39
33_R61_V0.6500.39
6_I170_A0.6480.39
119_A203_T0.6440.38
32_T213_W0.6440.38
103_T196_A0.6420.38
41_G63_F0.6350.38
193_K214_Y0.6330.37
37_L145_Y0.6320.37
183_S186_S0.6300.37
135_K157_L0.6300.37
84_D100_S0.6270.37
69_E114_A0.6260.37
133_L150_S0.6250.37
145_Y208_F0.6240.37
118_V142_Y0.6220.37
37_L161_T0.6220.37
79_V87_T0.6140.36
69_E113_D0.6130.36
105_Q152_P0.6130.36
170_A183_S0.6110.36
15_A197_R0.6090.35
174_Y177_V0.6090.35
23_G104_I0.6080.35
18_Q26_P0.6040.35
11_G70_L0.6010.35
66_R114_A0.6000.35
135_K169_K0.6000.35
124_P180_D0.6000.35
4_Y174_Y0.6000.35
47_Q134_E0.5990.35
7_L157_L0.5980.35
142_Y149_R0.5980.35
105_Q130_I0.5870.34
82_L85_Y0.5860.34
47_Q209_A0.5800.33
174_Y196_A0.5770.33
81_G86_H0.5750.33
180_D193_K0.5740.33
122_L129_V0.5720.32
16_W20_T0.5680.32
35_G42_A0.5680.32
10_A149_R0.5680.32
4_Y150_S0.5650.32
124_P132_E0.5650.32
69_E176_P0.5650.32
64_A119_A0.5640.32
20_T90_G0.5640.32
49_D203_T0.5620.32
29_R44_L0.5620.32
42_A120_L0.5620.32
14_Q22_E0.5610.32
4_Y18_Q0.5600.31
102_E208_F0.5580.31
70_L110_Y0.5570.31
12_P16_W0.5570.31
90_G94_D0.5560.31
29_R70_L0.5490.31
47_Q215_V0.5480.31
63_F182_Y0.5470.30
68_D114_A0.5460.30
21_F109_E0.5460.30
89_L92_R0.5440.30
54_L200_P0.5430.30
35_G116_F0.5430.30
46_I191_H0.5420.30
23_G133_L0.5420.30
11_G18_Q0.5400.30
151_C203_T0.5390.30
32_T189_G0.5390.30
56_A172_L0.5380.30
181_I184_E0.5380.30
201_M217_K0.5380.30
35_G160_G0.5380.30
159_L174_Y0.5360.30
141_R213_W0.5360.30
46_I58_S0.5350.30
141_R215_V0.5330.29
48_R79_V0.5320.29
68_D175_E0.5300.29
27_T116_F0.5290.29
138_L195_T0.5280.29
6_I111_L0.5280.29
19_P28_G0.5280.29
151_C170_A0.5260.29
55_Q120_L0.5250.29
161_T180_D0.5250.29
91_A156_P0.5240.29
36_L57_L0.5230.29
67_C116_F0.5210.28
41_G50_D0.5210.28
10_A110_Y0.5200.28
131_S180_D0.5160.28
9_L151_C0.5160.28
189_G201_M0.5150.28
192_L214_Y0.5150.28
137_V181_I0.5130.28
62_Q173_N0.5120.28
161_T174_Y0.5110.28
107_W144_P0.5080.27
8_R119_A0.5070.27
120_L187_V0.5060.27
8_R36_L0.5050.27
47_Q203_T0.5050.27
132_E139_K0.5050.27
19_P132_E0.5040.27
12_P27_T0.5030.27
164_A173_N0.5020.27
44_L155_H0.5020.27
106_T207_Q0.5000.27
12_P136_A0.5000.27
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4u7uK 1 0.9286 100 0.288 Contact Map
4n77A 1 0.8214 100 0.342 Contact Map
3vziA 2 0.8571 99.3 0.815 Contact Map
3kg4A 1 0.7455 99 0.84 Contact Map
3vzhA 2 0.7232 97.5 0.893 Contact Map
4f3mA 2 0.8438 96.5 0.908 Contact Map
4pwuA 4 0.317 9.2 0.966 Contact Map
2comA 1 0.1875 4.7 0.97 Contact Map
3o2iA 2 0.1741 4.3 0.971 Contact Map
1amlA 1 0.1473 2.6 0.974 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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