GREMLIN Database
CAS6 - CRISPR system Cascade subunit CasE
UniProt: Q46897 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13115
Length: 199 (187)
Sequences: 652 (552)
Seq/√Len: 40.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
135_R168_T5.2791.00
27_F56_S3.8241.00
17_Y189_M3.6321.00
113_R185_P3.6031.00
89_R162_C3.0151.00
3_L55_Q2.9711.00
78_L169_I2.8361.00
175_L196_L2.7881.00
134_A167_L2.7731.00
133_A183_I2.7231.00
123_I139_V2.6911.00
88_L126_L2.6081.00
130_L134_A2.5791.00
186_A191_C2.5271.00
90_A163_F2.4781.00
83_P168_T2.3441.00
178_L183_I2.3251.00
183_I194_L2.2421.00
93_I185_P2.2301.00
17_Y21_Q2.2201.00
124_A128_R2.2141.00
133_A178_L2.2071.00
87_R164_E2.2020.99
116_L121_E2.1150.99
167_L175_L2.0910.99
127_Q136_V2.0090.99
24_W37_F1.9890.99
177_D181_Q1.9480.99
71_T75_E1.8950.98
80_V172_A1.7640.98
72_K76_F1.7280.97
137_E168_T1.7130.97
175_L194_L1.6810.97
118_K121_E1.6780.97
84_L167_L1.6780.97
160_T191_C1.6110.96
96_I114_V1.6090.96
127_Q130_L1.4880.93
24_W189_M1.4440.92
14_R187_K1.4400.92
131_G135_R1.4170.92
3_L53_L1.4130.91
92_P161_V1.3380.89
18_Q21_Q1.3340.89
55_Q195_S1.3320.89
183_I196_L1.2970.88
17_Y24_W1.2810.87
87_R195_S1.2800.87
23_L52_V1.2690.86
99_N102_R1.2220.84
86_F126_L1.2120.84
83_P137_E1.2110.84
169_I172_A1.2030.83
142_I164_E1.1950.83
90_A126_L1.1930.83
66_A69_I1.1930.83
129_K183_I1.1770.82
129_K132_N1.1620.81
5_K120_A1.1570.81
42_E156_G1.1570.81
95_T110_K1.1540.81
174_A177_D1.1520.80
18_Q45_N1.1390.80
37_F189_M1.1380.80
99_N110_K1.1360.79
32_D35_R1.1230.79
90_A125_W1.1180.78
36_D39_F1.1130.78
179_V196_L1.1090.78
26_L39_F1.1030.77
93_I112_C1.0960.77
145_R187_K1.0920.77
36_D57_A1.0900.77
134_A175_L1.0790.76
27_F37_F1.0670.75
158_I191_C1.0520.74
113_R116_L1.0270.72
115_P188_S1.0200.72
74_V194_L1.0190.72
72_K75_E1.0160.71
37_F191_C1.0150.71
5_K28_P1.0110.71
122_Q163_F1.0040.70
89_R160_T0.9950.70
2_Y7_I0.9920.69
172_A175_L0.9760.68
23_L37_F0.9750.68
6_V26_L0.9720.68
102_R110_K0.9690.68
70_K131_G0.9680.68
22_G186_A0.9670.67
129_K133_A0.9620.67
31_P54_L0.9610.67
71_T195_S0.9590.67
78_L84_L0.9560.67
54_L62_S0.9550.66
169_I175_L0.9490.66
75_E176_I0.9450.66
65_V69_I0.9440.66
26_L62_S0.9370.65
150_S153_G0.9260.64
122_Q161_V0.9230.64
89_R144_E0.9170.63
120_A124_A0.9090.63
98_D110_K0.9010.62
78_L175_L0.9010.62
172_A176_I0.8940.61
171_D174_A0.8820.60
72_K89_R0.8790.60
4_S84_L0.8710.59
5_K70_K0.8680.59
119_E122_Q0.8630.59
133_A174_A0.8630.59
141_P161_V0.8540.58
98_D102_R0.8520.58
19_L39_F0.8510.57
32_D36_D0.8500.57
37_F125_W0.8500.57
126_L133_A0.8490.57
23_L189_M0.8490.57
86_F167_L0.8480.57
39_F110_K0.8460.57
12_W22_G0.8440.57
4_S62_S0.8420.57
161_V167_L0.8380.56
121_E191_C0.8360.56
22_G69_I0.8310.56
5_K51_H0.8310.56
12_W149_F0.8280.55
124_A170_N0.8270.55
70_K76_F0.8040.53
79_Q154_K0.7960.52
179_V195_S0.7940.52
57_A177_D0.7940.52
57_A176_I0.7880.52
99_N171_D0.7810.51
162_C195_S0.7760.51
94_K114_V0.7740.50
56_S191_C0.7720.50
125_W186_A0.7670.50
22_G149_F0.7580.49
96_I115_P0.7580.49
38_L55_Q0.7530.49
130_L194_L0.7530.49
176_I180_Q0.7510.48
42_E55_Q0.7500.48
186_A193_L0.7500.48
18_Q24_W0.7440.48
52_V162_C0.7430.48
74_V82_V0.7430.48
36_D58_Q0.7410.47
8_I59_M0.7340.47
158_I183_I0.7300.46
63_T169_I0.7290.46
40_H195_S0.7290.46
162_C197_A0.7280.46
45_N83_P0.7270.46
34_A77_Q0.7240.46
39_F132_N0.7240.46
6_V9_A0.7190.45
152_D171_D0.7180.45
18_Q22_G0.7180.45
135_R170_N0.7160.45
85_Y197_A0.7130.45
65_V87_R0.7130.45
27_F191_C0.7120.45
73_Q145_R0.7060.44
84_L134_A0.7050.44
84_L196_L0.7040.44
149_F156_G0.6960.43
98_D181_Q0.6940.43
12_W64_A0.6920.43
45_N164_E0.6860.42
19_L41_V0.6750.41
26_L42_E0.6740.41
19_L36_D0.6690.41
151_G154_K0.6680.41
14_R128_R0.6680.41
100_Q143_S0.6660.41
17_Y188_S0.6630.40
117_I121_E0.6620.40
24_W34_A0.6590.40
42_E87_R0.6560.40
2_Y155_S0.6540.40
43_K50_C0.6530.39
82_V85_Y0.6530.39
42_E122_Q0.6530.39
151_G155_S0.6460.39
111_R114_V0.6440.39
83_P155_S0.6370.38
116_L119_E0.6320.38
35_R74_V0.6320.38
84_L92_P0.6310.38
51_H59_M0.6310.38
53_L145_R0.6280.37
115_P122_Q0.6230.37
35_R89_R0.6210.37
36_D195_S0.6200.37
130_L136_V0.6190.37
59_M168_T0.6180.36
122_Q126_L0.6160.36
93_I96_I0.6160.36
27_F93_I0.6150.36
42_E196_L0.6140.36
39_F134_A0.6110.36
23_L169_I0.6100.36
150_S155_S0.6080.36
55_Q175_L0.6060.35
9_A13_S0.6060.35
119_E123_I0.6030.35
132_N155_S0.6020.35
146_P159_Q0.6000.35
146_P161_V0.5990.35
36_D122_Q0.5930.34
23_L195_S0.5900.34
134_A188_S0.5880.34
8_I139_V0.5860.34
57_A68_V0.5850.34
2_Y172_A0.5800.33
45_N48_E0.5780.33
72_K153_G0.5770.33
43_K124_A0.5770.33
17_Y92_P0.5770.33
80_V135_R0.5750.33
141_P167_L0.5740.33
146_P163_F0.5720.33
22_G185_P0.5710.33
5_K185_P0.5700.32
16_L147_Q0.5680.32
143_S152_D0.5680.32
126_L167_L0.5680.32
170_N181_Q0.5680.32
28_P61_V0.5660.32
88_L112_C0.5660.32
86_F134_A0.5640.32
95_T111_R0.5600.32
24_W35_R0.5580.31
41_V52_V0.5570.31
176_I196_L0.5570.31
177_D188_S0.5560.31
22_G25_H0.5550.31
27_F180_Q0.5550.31
29_N58_Q0.5540.31
9_A15_D0.5530.31
2_Y187_K0.5440.30
111_R172_A0.5430.30
42_E52_V0.5420.30
18_Q55_Q0.5410.30
184_G192_G0.5400.30
34_A196_L0.5400.30
44_R194_L0.5380.30
178_L194_L0.5360.30
84_L189_M0.5350.30
165_G184_G0.5330.30
19_L37_F0.5330.30
99_N193_L0.5320.29
135_R163_F0.5290.29
70_K75_E0.5270.29
113_R139_V0.5270.29
39_F65_V0.5260.29
137_E150_S0.5220.29
130_L175_L0.5210.29
59_M132_N0.5210.29
50_C124_A0.5180.28
175_L183_I0.5180.28
160_T186_A0.5120.28
33_A124_A0.5110.28
11_A25_H0.5110.28
54_L99_N0.5110.28
126_L130_L0.5100.28
95_T185_P0.5080.28
13_S196_L0.5060.28
64_A67_T0.5060.28
123_I127_Q0.5040.27
3_L74_V0.5040.27
63_T153_G0.5030.27
90_A122_Q0.5030.27
2_Y123_I0.5030.27
89_R196_L0.5020.27
125_W193_L0.5000.27
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4dzdA 1 0.9648 100 0.119 Contact Map
3qrpA 1 0.9397 100 0.174 Contact Map
2y8yA 1 0.8945 100 0.176 Contact Map
4c97A 1 0.8894 50 0.941 Contact Map
3i4hX 1 0.8593 23.7 0.95 Contact Map
3ufcX 1 0.9045 16.3 0.954 Contact Map
3cymA 1 0.2663 4.3 0.965 Contact Map
4illA 2 0.8995 4.1 0.965 Contact Map
4uvqA 2 0.2864 3.6 0.966 Contact Map
4c98A 2 0.8241 2.8 0.968 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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