GREMLIN Database
CAS1 - CRISPR-associated endonuclease Cas1
UniProt: Q46896 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13114
Length: 305 (270)
Sequences: 2430 (1780)
Seq/√Len: 108.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
183_A228_V5.3971.00
183_A260_T4.8961.00
104_K107_L3.3521.00
128_A137_L3.2051.00
242_E259_K3.1911.00
101_Y104_K3.1231.00
242_E256_R3.1001.00
253_D256_R3.0011.00
26_D35_I2.8991.00
65_V75_L2.7831.00
188_Y220_A2.7811.00
69_A86_Y2.6861.00
168_K173_G2.6081.00
135_E138_R2.5961.00
100_L210_G2.5621.00
139_G142_G2.5461.00
172_K181_S2.4751.00
242_E253_D2.4661.00
273_A280_P2.4421.00
252_R256_R2.3501.00
133_S136_Q2.2921.00
27_V67_L2.2721.00
190_V264_L2.2561.00
186_C264_L2.2551.00
46_V73_T2.2071.00
99_L201_A2.2011.00
24_Q59_R2.1871.00
134_V138_R2.1771.00
183_A232_A2.1161.00
46_V67_L2.0631.00
187_L228_V2.0021.00
179_C236_A1.9551.00
67_L71_V1.9251.00
100_L106_A1.9101.00
53_M78_V1.8741.00
113_L117_R1.8731.00
191_T220_A1.8571.00
263_K266_P1.8541.00
176_I236_A1.7981.00
96_S204_I1.7431.00
115_V119_M1.7421.00
32_F64_A1.7341.00
262_A266_P1.7081.00
267_L270_D1.6971.00
31_A43_H1.6681.00
148_T229_V1.6311.00
105_L111_L1.6161.00
25_I64_A1.6141.00
99_L103_A1.6101.00
108_D111_L1.5981.00
235_I263_K1.5931.00
78_V197_A1.5781.00
132_R136_Q1.5681.00
33_V43_H1.5681.00
101_Y105_L1.5581.00
266_P269_E1.5441.00
32_F46_V1.5201.00
52_I68_A1.5111.00
113_L137_L1.5031.00
254_I258_S1.4801.00
231_K235_I1.4671.00
36_D41_R1.4601.00
32_F52_I1.4591.00
232_A260_T1.4511.00
279_P285_D1.4171.00
147_A150_A1.4111.00
176_I232_A1.4091.00
32_F68_A1.3961.00
175_T240_P1.3691.00
24_Q61_S1.3641.00
36_D39_G1.3601.00
153_A174_D1.3541.00
20_L261_L1.3441.00
179_C232_A1.3441.00
17_M52_I1.3371.00
242_E252_R1.3341.00
220_A224_K1.3331.00
171_E181_S1.3320.99
273_A278_Q1.3290.99
54_L58_T1.3270.99
66_R85_V1.3080.99
119_M122_L1.3070.99
28_I43_H1.3070.99
254_I257_S1.2940.99
120_F207_V1.2890.99
235_I259_K1.2820.99
272_L286_A1.2700.99
184_T229_V1.2700.99
138_R209_T1.2510.99
64_A68_A1.2250.99
134_V209_T1.2220.99
68_A75_L1.2190.99
112_R209_T1.2030.99
57_G63_A1.1960.99
160_W240_P1.1950.99
18_I55_E1.1870.99
223_I286_A1.1860.99
195_I216_V1.1770.99
79_G83_V1.1740.99
283_P286_A1.1700.99
118_K121_E1.1650.99
27_V46_V1.1640.99
264_L268_I1.1560.99
63_A66_R1.1540.99
265_I284_E1.1420.98
111_L114_K1.1370.98
271_V280_P1.1250.98
171_E178_Q1.1060.98
76_V87_A1.1050.98
143_S146_R1.0890.98
195_I219_I1.0880.98
140_I143_S1.0750.98
192_E216_V1.0670.98
152_L176_I1.0640.98
52_I75_L1.0510.97
121_E129_P1.0450.97
190_V268_I1.0410.97
23_G58_T1.0370.97
113_L131_R1.0320.97
183_A231_K1.0320.97
182_A260_T1.0210.97
94_A212_P1.0180.97
182_A257_S1.0160.97
113_L133_S1.0150.97
182_A261_L1.0090.97
242_E255_F1.0050.96
179_C252_R1.0000.96
40_I43_H0.9970.96
226_D283_P0.9960.96
35_I40_I0.9890.96
59_R63_A0.9880.96
46_V71_V0.9850.96
239_N242_E0.9780.96
138_R207_V0.9750.96
145_V229_V0.9740.96
60_V64_A0.9730.96
180_I232_A0.9720.96
16_S53_M0.9710.96
126_E129_P0.9690.96
184_T224_K0.9670.96
26_D33_V0.9610.95
124_F147_A0.9600.95
153_A162_G0.9570.95
143_S147_A0.9560.95
190_V284_E0.9530.95
30_G71_V0.9520.95
101_Y111_L0.9490.95
214_S217_Y0.9470.95
60_V65_V0.9340.95
123_R127_P0.9330.95
161_N236_A0.9290.95
169_D172_K0.9230.94
187_L220_A0.9210.94
17_M49_V0.9150.94
30_G67_L0.9120.94
228_V232_A0.9120.94
274_A277_I0.9050.94
270_D273_A0.8980.94
234_E238_R0.8940.93
142_G221_D0.8930.93
100_L103_A0.8890.93
191_T219_I0.8870.93
32_F73_T0.8850.93
76_V189_G0.8830.93
117_R131_R0.8820.93
106_A112_R0.8810.93
84_R87_A0.8800.93
158_V161_N0.8780.93
170_W178_Q0.8740.93
194_A198_A0.8630.92
115_V118_K0.8590.92
125_G129_P0.8590.92
100_L107_L0.8580.92
119_M222_I0.8560.92
272_L281_A0.8540.92
116_V138_R0.8520.92
128_A131_R0.8520.92
22_Y57_G0.8510.92
51_C74_L0.8390.91
26_D61_S0.8340.91
186_C261_L0.8320.91
282_P285_D0.8310.91
182_A185_S0.8290.90
114_K118_K0.8260.90
170_W217_Y0.8260.90
94_A201_A0.8240.90
281_A286_A0.8200.90
271_V282_P0.8160.90
175_T244_D0.8130.90
179_C260_T0.8120.90
156_Y176_I0.8090.89
188_Y217_Y0.8020.89
121_E125_G0.7990.89
261_L265_I0.7990.89
149_Y184_T0.7990.89
124_F144_R0.7970.89
117_R128_A0.7860.88
152_L155_Q0.7860.88
122_L222_I0.7830.88
178_Q250_A0.7810.88
219_I222_I0.7780.87
118_K122_L0.7750.87
68_A94_A0.7740.87
21_Q36_D0.7730.87
22_Y63_A0.7730.87
146_R150_A0.7730.87
152_L156_Y0.7710.87
131_R136_Q0.7690.87
95_R98_K0.7680.87
125_G128_A0.7680.87
180_I184_T0.7660.87
33_V40_I0.7650.87
46_V68_A0.7570.86
67_L73_T0.7540.86
141_E208_H0.7490.85
37_K147_A0.7490.85
136_Q140_I0.7480.85
22_Y80_E0.7470.85
185_S189_G0.7440.85
16_S51_C0.7430.85
99_L203_A0.7410.85
100_L209_T0.7410.85
124_F140_I0.7410.85
147_A241_G0.7360.84
217_Y220_A0.7340.84
120_F137_L0.7340.84
110_D114_K0.7330.84
60_V75_L0.7310.84
274_A281_A0.7300.84
151_L154_K0.7280.84
130_A136_Q0.7260.84
65_V77_W0.7230.83
113_L209_T0.7210.83
188_Y192_E0.7190.83
163_R167_P0.7160.83
39_G42_T0.7150.83
127_P130_A0.7120.82
175_T250_A0.7090.82
120_F128_A0.7070.82
97_D101_Y0.7070.82
221_D224_K0.7060.82
85_V88_S0.7040.82
194_A197_A0.7030.82
51_C76_V0.6990.81
99_L212_P0.6980.81
243_P247_V0.6970.81
203_A216_V0.6960.81
167_P172_K0.6940.81
66_R70_Q0.6920.81
114_K117_R0.6920.81
153_A164_R0.6900.80
259_K263_K0.6870.80
171_E250_A0.6870.80
210_G214_S0.6860.80
234_E267_L0.6800.79
106_A204_I0.6800.79
259_K262_A0.6780.79
60_V77_W0.6770.79
103_A204_I0.6720.79
117_R121_E0.6700.78
106_A206_F0.6700.78
109_E126_E0.6690.78
94_A200_Y0.6680.78
61_S64_A0.6650.78
201_A212_P0.6620.78
137_L207_V0.6590.77
260_T263_K0.6580.77
171_E256_R0.6510.76
244_D251_C0.6490.76
116_V207_V0.6470.76
27_V64_A0.6440.76
255_F259_K0.6430.76
117_R129_P0.6430.76
112_R134_V0.6420.75
19_F64_A0.6400.75
145_V184_T0.6400.75
250_A257_S0.6380.75
119_M219_I0.6370.75
187_L264_L0.6340.75
137_L162_G0.6320.74
168_K172_K0.6320.74
69_A88_S0.6270.74
267_L283_P0.6230.73
183_A247_V0.6210.73
203_A211_K0.6210.73
96_S100_L0.6210.73
72_G198_A0.6200.73
188_Y224_K0.6190.73
28_I33_V0.6180.73
205_G210_G0.6120.72
48_S150_A0.6090.72
100_L104_K0.6030.71
79_G85_V0.6020.71
209_T214_S0.6010.71
132_R266_P0.5990.70
192_E195_I0.5970.70
44_I52_I0.5960.70
98_K212_P0.5950.70
201_A205_G0.5930.70
102_Q214_S0.5920.69
22_Y55_E0.5920.69
236_A239_N0.5910.69
100_L204_I0.5900.69
105_L206_F0.5880.69
169_D225_F0.5880.69
57_G80_E0.5880.69
266_P270_D0.5870.69
121_E128_A0.5870.69
99_L213_L0.5840.68
27_V30_G0.5840.68
119_M206_F0.5840.68
96_S103_A0.5840.68
65_V69_A0.5780.68
22_Y58_T0.5770.68
128_A251_C0.5770.68
139_G176_I0.5770.68
223_I267_L0.5760.67
169_D181_S0.5750.67
161_N179_C0.5740.67
65_V88_S0.5730.67
166_D176_I0.5720.67
281_A285_D0.5710.67
76_V84_R0.5690.66
183_A187_L0.5680.66
105_L115_V0.5660.66
173_G249_L0.5650.66
204_I210_G0.5650.66
116_V137_L0.5650.66
185_S217_Y0.5640.66
94_A203_A0.5640.66
18_I265_I0.5610.65
121_E126_E0.5580.65
118_K240_P0.5580.65
75_L88_S0.5580.65
117_R273_A0.5570.65
61_S199_G0.5540.64
184_T220_A0.5530.64
171_E243_P0.5520.64
159_T278_Q0.5500.64
156_Y236_A0.5500.64
231_K263_K0.5490.64
148_T167_P0.5470.63
106_A209_T0.5470.63
140_I147_A0.5460.63
149_Y221_D0.5450.63
255_F271_V0.5440.63
124_F143_S0.5430.63
131_R140_I0.5420.63
116_V215_F0.5420.63
192_E202_P0.5410.63
24_Q68_A0.5410.63
23_G34_L0.5410.63
276_E279_P0.5400.62
156_Y238_R0.5400.62
179_C243_P0.5390.62
181_S185_S0.5350.62
235_I260_T0.5340.62
158_V162_G0.5340.62
116_V134_V0.5330.61
36_D127_P0.5330.61
22_Y56_P0.5330.61
69_A74_L0.5320.61
167_P171_E0.5310.61
192_E219_I0.5300.61
17_M32_F0.5290.61
135_E139_G0.5270.61
103_A201_A0.5260.60
64_A67_L0.5260.60
263_K270_D0.5260.60
15_V48_S0.5230.60
275_G281_A0.5220.60
180_I199_G0.5220.60
47_G71_V0.5210.60
175_T239_N0.5210.60
147_A151_L0.5200.60
181_S206_F0.5200.60
28_I40_I0.5190.59
126_E147_A0.5180.59
203_A210_G0.5170.59
239_N259_K0.5160.59
114_K260_T0.5160.59
50_A203_A0.5150.59
38_T42_T0.5140.59
74_L193_A0.5130.59
30_G47_G0.5120.58
119_M221_D0.5110.58
205_G212_P0.5110.58
116_V119_M0.5090.58
187_L223_I0.5090.58
145_V224_K0.5080.58
126_E162_G0.5080.58
178_Q237_R0.5070.58
77_W88_S0.5070.58
74_L87_A0.5070.58
195_I200_Y0.5070.58
113_L128_A0.5060.58
100_L112_R0.5060.58
46_V64_A0.5040.57
68_A73_T0.5030.57
21_Q91_P0.5030.57
24_Q161_N0.5020.57
29_D43_H0.5020.57
149_Y233_F0.5010.57
102_Q206_F0.5000.57
115_V206_F0.5000.57
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4xtkA 2 0.9738 100 0.18 Contact Map
2yzsA 4 0.9344 100 0.181 Contact Map
4wj0A 2 0.941 100 0.186 Contact Map
4n06A 2 0.9443 100 0.214 Contact Map
3nkdA 2 0.8623 100 0.285 Contact Map
4w8kA 2 0.859 100 0.297 Contact Map
3godA 4 0.8852 100 0.328 Contact Map
3nkeA 3 0.6033 100 0.512 Contact Map
2iojA 1 0.3279 47.5 0.957 Contact Map
4bgvA 4 0.7607 25.3 0.963 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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