YQGA - ECOLI - GREMLIN Contacts

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YQGA - Uncharacterized protein YqgA

UniProt:	Q46831	Sequences: MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLGIGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESFIQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVISIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLPALLLAMPLSAAWTAWFA	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG12987
Length:	235 (223)
Sequences:	997 (799)
Seq/√Len:	53.5

ECOLI - YQGA
BACSU - YDFK

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
67_A	216_M	5.708	1.00
7_I	136_I	4.915	1.00
126_E	132_P	4.042	1.00
74_L	77_K	3.774	1.00
11_A	139_S	3.465	1.00
12_V	164_L	3.437	1.00
9_A	165_L	3.325	1.00
78_G	81_T	3.024	1.00
71_E	215_N	2.916	1.00
44_G	196_L	2.889	1.00
5_P	168_Q	2.860	1.00
49_L	195_G	2.854	1.00
6_F	10_S	2.830	1.00
125_N	131_D	2.754	1.00
69_I	222_L	2.638	1.00
76_E	214_V	2.572	1.00
37_I	63_T	2.550	1.00
125_N	129_T	2.415	1.00
43_L	202_G	2.277	1.00
3_I	136_I	2.226	1.00
16_G	158_S	2.204	1.00
41_A	63_T	2.155	1.00
69_I	73_C	2.128	1.00
33_S	67_A	2.098	1.00
12_V	119_G	1.949	1.00
5_P	172_A	1.946	1.00
204_R	209_K	1.917	1.00
105_N	109_I	1.863	0.99
222_L	226_L	1.795	0.99
203_L	208_I	1.792	0.99
65_L	222_L	1.790	0.99
83_V	214_V	1.774	0.99
123_A	127_G	1.752	0.99
207_G	210_M	1.739	0.99
44_G	50_V	1.693	0.99
63_T	196_L	1.688	0.99
28_E	31_R	1.658	0.99
112_L	149_F	1.657	0.99
113_F	146_A	1.629	0.98
155_I	159_V	1.616	0.98
102_F	159_V	1.611	0.98
58_A	226_L	1.602	0.98
28_E	32_V	1.580	0.98
197_L	219_A	1.574	0.98
30_I	72_I	1.530	0.98
109_I	149_F	1.512	0.97
121_F	134_I	1.511	0.97
101_S	104_Q	1.502	0.97
79_V	214_V	1.495	0.97
5_P	126_E	1.488	0.97
32_V	36_S	1.478	0.97
119_G	167_I	1.471	0.97
27_P	30_I	1.462	0.97
18_L	147_M	1.459	0.97
84_A	103_I	1.454	0.97
106_Y	110_I	1.449	0.97
13_L	158_S	1.437	0.96
132_P	136_I	1.378	0.95
41_A	60_V	1.376	0.95
9_A	161_S	1.352	0.95
211_F	216_M	1.345	0.95
77_K	81_T	1.297	0.94
223_A	226_L	1.292	0.94
62_A	222_L	1.283	0.93
53_C	189_D	1.275	0.93
134_I	137_A	1.246	0.92
76_E	80_N	1.241	0.92
38_F	145_T	1.228	0.92
124_M	128_M	1.220	0.91
40_L	200_A	1.219	0.91
126_E	172_A	1.211	0.91
223_A	227_S	1.202	0.91
124_M	171_L	1.169	0.90
193_V	223_A	1.124	0.88
75_L	214_V	1.093	0.86
105_N	153_L	1.092	0.86
64_L	144_F	1.089	0.86
165_L	169_L	1.083	0.86
146_A	149_F	1.070	0.85
29_R	71_E	1.065	0.85
119_G	163_P	1.063	0.85
212_P	224_M	1.060	0.84
83_V	107_V	1.052	0.84
113_F	147_M	1.043	0.83
60_V	137_A	1.039	0.83
161_S	164_L	1.034	0.83
87_Q	102_F	1.030	0.83
11_A	143_F	1.027	0.82
189_D	192_A	1.023	0.82
83_V	110_I	1.016	0.82
170_T	173_W	1.015	0.82
7_I	132_P	1.014	0.82
37_I	67_A	1.012	0.82
183_T	186_M	1.009	0.81
84_A	87_Q	1.005	0.81
133_S	137_A	0.993	0.80
47_I	50_V	0.993	0.80
162_I	166_I	0.990	0.80
33_S	71_E	0.987	0.80
42_S	119_G	0.982	0.80
168_Q	220_L	0.981	0.79
16_G	150_A	0.978	0.79
136_I	165_L	0.978	0.79
7_I	139_S	0.966	0.78
45_I	56_L	0.964	0.78
42_S	145_T	0.957	0.78
52_K	146_A	0.953	0.77
23_S	31_R	0.952	0.77
13_L	17_V	0.952	0.77
189_D	227_S	0.946	0.77
161_S	165_L	0.946	0.77
7_I	11_A	0.944	0.77
39_G	205_I	0.941	0.77
110_I	164_L	0.933	0.76
50_V	199_L	0.928	0.76
120_I	190_F	0.920	0.75
136_I	139_S	0.919	0.75
85_K	89_L	0.917	0.75
4_G	135_L	0.915	0.74
221_L	224_M	0.913	0.74
4_G	126_E	0.909	0.74
2_V	172_A	0.903	0.74
22_L	25_R	0.901	0.73
85_K	88_N	0.901	0.73
55_N	189_D	0.895	0.73
123_A	171_L	0.893	0.73
129_T	134_I	0.890	0.72
58_A	223_A	0.890	0.72
145_T	148_I	0.880	0.72
20_A	158_S	0.878	0.71
61_L	222_L	0.878	0.71
118_T	134_I	0.874	0.71
2_V	132_P	0.873	0.71
69_I	72_I	0.872	0.71
141_L	145_T	0.865	0.70
180_P	183_T	0.862	0.70
143_F	146_A	0.855	0.69
192_A	195_G	0.846	0.69
29_R	74_L	0.845	0.68
25_R	148_I	0.844	0.68
131_D	134_I	0.844	0.68
11_A	141_L	0.833	0.67
198_L	201_T	0.824	0.66
28_E	183_T	0.823	0.66
53_C	196_L	0.822	0.66
117_G	121_F	0.822	0.66
74_L	78_G	0.821	0.66
33_S	37_I	0.819	0.66
124_M	127_G	0.819	0.66
49_L	223_A	0.817	0.66
113_F	195_G	0.814	0.66
48_L	52_K	0.812	0.65
72_I	225_P	0.808	0.65
47_I	199_L	0.807	0.65
14_L	143_F	0.806	0.65
32_V	212_P	0.799	0.64
42_S	205_I	0.795	0.64
75_L	78_G	0.793	0.64
141_L	148_I	0.792	0.63
87_Q	103_I	0.789	0.63
186_M	190_F	0.788	0.63
13_L	161_S	0.788	0.63
17_V	77_K	0.786	0.63
58_A	193_V	0.771	0.61
33_S	36_S	0.765	0.61
203_L	209_K	0.765	0.61
71_E	212_P	0.764	0.61
112_L	167_I	0.764	0.61
125_N	134_I	0.755	0.60
41_A	195_G	0.750	0.59
219_A	223_A	0.749	0.59
42_S	198_L	0.749	0.59
44_G	63_T	0.741	0.58
111_V	198_L	0.729	0.57
61_L	65_L	0.727	0.57
26_L	33_S	0.724	0.56
203_L	211_F	0.723	0.56
74_L	81_T	0.723	0.56
86_A	159_V	0.721	0.56
114_C	183_T	0.713	0.55
174_A	177_L	0.708	0.55
102_F	156_A	0.708	0.55
59_M	196_L	0.707	0.55
5_P	9_A	0.704	0.54
186_M	223_A	0.703	0.54
168_Q	172_A	0.697	0.54
146_A	150_A	0.695	0.53
104_Q	210_M	0.695	0.53
81_T	85_K	0.694	0.53
49_L	134_I	0.693	0.53
120_I	186_M	0.689	0.53
10_S	14_L	0.689	0.53
41_A	198_L	0.688	0.53
204_R	210_M	0.688	0.53
190_F	221_L	0.687	0.53
123_A	172_A	0.685	0.52
10_S	65_L	0.684	0.52
23_S	61_L	0.680	0.52
87_Q	104_Q	0.680	0.52
33_S	216_M	0.675	0.51
157_V	167_I	0.673	0.51
17_V	170_T	0.672	0.51
113_F	143_F	0.672	0.51
45_I	187_M	0.670	0.51
109_I	113_F	0.669	0.51
46_G	202_G	0.666	0.50
52_K	208_I	0.666	0.50
186_M	224_M	0.665	0.50
145_T	196_L	0.663	0.50
26_L	153_L	0.662	0.50
48_L	193_V	0.661	0.50
16_G	155_I	0.660	0.50
73_C	78_G	0.659	0.50
174_A	180_P	0.657	0.49
9_A	13_L	0.656	0.49
38_F	64_L	0.655	0.49
79_V	111_V	0.654	0.49
61_L	140_F	0.653	0.49
14_L	77_K	0.650	0.49
169_L	173_W	0.649	0.49
199_L	202_G	0.648	0.48
54_A	189_D	0.646	0.48
180_P	191_S	0.645	0.48
40_L	196_L	0.644	0.48
87_Q	124_M	0.644	0.48
105_N	149_F	0.643	0.48
110_I	123_A	0.643	0.48
38_F	149_F	0.642	0.48
108_A	215_N	0.641	0.48
39_G	202_G	0.641	0.48
105_N	157_V	0.639	0.48
16_G	146_A	0.639	0.48
141_L	149_F	0.639	0.48
83_V	102_F	0.637	0.47
34_M	144_F	0.637	0.47
18_L	45_I	0.633	0.47
178_I	181_L	0.631	0.47
43_L	184_P	0.628	0.46
201_T	214_V	0.626	0.46
170_T	174_A	0.625	0.46
4_G	132_P	0.622	0.46
113_F	157_V	0.618	0.45
35_T	153_L	0.617	0.45
20_A	124_M	0.614	0.45
196_L	199_L	0.612	0.45
44_G	195_G	0.610	0.44
160_I	164_L	0.609	0.44
117_G	145_T	0.606	0.44
11_A	166_I	0.604	0.44
16_G	20_A	0.603	0.44
160_I	163_P	0.600	0.43
76_E	215_N	0.599	0.43
54_A	185_S	0.599	0.43
59_M	189_D	0.597	0.43
151_C	155_I	0.597	0.43
125_N	172_A	0.597	0.43
203_L	212_P	0.596	0.43
64_L	175_A	0.594	0.43
54_A	117_G	0.594	0.43
47_I	51_V	0.592	0.43
69_I	77_K	0.592	0.43
73_C	77_K	0.587	0.42
150_A	158_S	0.587	0.42
14_L	101_S	0.587	0.42
106_Y	156_A	0.586	0.42
6_F	9_A	0.586	0.42
139_S	143_F	0.584	0.42
6_F	191_S	0.579	0.41
209_K	212_P	0.575	0.41
114_C	137_A	0.575	0.41
184_P	188_A	0.573	0.41
127_G	134_I	0.573	0.41
41_A	141_L	0.573	0.41
201_T	213_V	0.572	0.41
177_L	181_L	0.572	0.41
62_A	137_A	0.570	0.40
52_K	76_E	0.564	0.40
14_L	208_I	0.563	0.40
180_P	219_A	0.562	0.40
78_G	151_C	0.562	0.40
26_L	37_I	0.561	0.39
31_R	76_E	0.560	0.39
11_A	81_T	0.560	0.39
56_L	139_S	0.558	0.39
49_L	118_T	0.556	0.39
214_V	217_L	0.554	0.39
175_A	217_L	0.551	0.38
119_G	145_T	0.550	0.38
88_N	98_A	0.549	0.38
47_I	222_L	0.549	0.38
44_G	48_L	0.548	0.38
173_W	219_A	0.547	0.38
20_A	155_I	0.547	0.38
57_P	213_V	0.546	0.38
115_A	198_L	0.546	0.38
36_S	206_C	0.546	0.38
173_W	177_L	0.543	0.38
146_A	157_V	0.543	0.38
123_A	163_P	0.538	0.37
113_F	140_F	0.537	0.37
114_C	177_L	0.536	0.37
182_T	187_M	0.536	0.37
63_T	174_A	0.535	0.37
33_S	153_L	0.531	0.36
31_R	39_G	0.531	0.36
34_M	167_I	0.530	0.36
39_G	163_P	0.527	0.36
149_F	186_M	0.525	0.36
6_F	98_A	0.524	0.36
206_C	220_L	0.522	0.36
145_T	149_F	0.521	0.35
14_L	65_L	0.521	0.35
26_L	148_I	0.521	0.35
99_H	102_F	0.521	0.35
16_G	161_S	0.520	0.35
17_V	65_L	0.520	0.35
102_F	120_I	0.517	0.35
156_A	160_I	0.517	0.35
62_A	91_R	0.516	0.35
144_F	223_A	0.516	0.35
112_L	157_V	0.515	0.35
2_V	205_I	0.514	0.35
105_N	193_V	0.511	0.34
16_G	113_F	0.510	0.34
32_V	207_G	0.509	0.34
127_G	138_K	0.508	0.34
27_P	78_G	0.508	0.34
60_V	117_G	0.506	0.34
35_T	152_S	0.505	0.34
22_L	84_A	0.504	0.34
209_K	216_M	0.503	0.34
78_G	140_F	0.503	0.34
34_M	115_A	0.500	0.33
76_E	212_P	0.500	0.33

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1t5jA	1	0.2255	33.2	0.958	Contact Map
3hfwA	1	0.2553	21.2	0.961	Contact Map
2nwlA	3	0.634	9.7	0.967	Contact Map
4eneA	2	0.3745	8.7	0.968	Contact Map
2xq2A	2	0.3234	7.9	0.968	Contact Map
3nd0A	2	0.366	6	0.97	Contact Map
4n7wA	2	0.366	4.2	0.972	Contact Map
3w9iA	3	0.3617	3.6	0.973	Contact Map
3zuxA	1	0.3106	3.5	0.973	Contact Map
4f35D	2	0.8213	3.4	0.973	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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