SSNA - ECOLI - GREMLIN Contacts

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SSNA - Protein SsnA

UniProt:	Q46812	Sequences: MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDASFKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEESLYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRNNGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVKATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLLNQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGGPLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIAFGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG13062
Length:	442 (357)
Sequences:	27373 (21036)
Seq/√Len:	1113.3

ECOLI - SSNA GUAD YAHJ CODA PHNM IADA ALLB PHYDA NAGA
BACSU - HUTI YTCJ ALLB NAGA PYRC
HALSA - MTAD Q9HQE0 HUTI Q9HNZ2 Q9HHS1 PYRC
SULSO - Q7LXN1 Q97V65 Q97WS6 Q97W09 Q97VT3 Q97XD4 Q980B7 PYRC

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
404_M	409_M	5.006	1.00
4_L	49_M	4.684	1.00
362_L	367_Y	4.645	1.00
52_R	401_H	3.951	1.00
402_S	409_M	3.809	1.00
26_E	31_V	3.665	1.00
49_M	373_I	3.609	1.00
336_L	339_D	3.532	1.00
7_V	49_M	3.415	1.00
57_G	120_T	3.241	1.00
364_E	367_Y	3.195	1.00
55_M	374_C	3.192	1.00
5_K	46_F	2.822	1.00
32_A	365_A	2.803	1.00
360_F	368_K	2.797	1.00
368_K	406_N	2.697	1.00
28_D	368_K	2.651	1.00
55_M	372_T	2.600	1.00
45_S	412_E	2.573	1.00
3_I	24_A	2.484	1.00
54_V	373_I	2.471	1.00
359_K	368_K	2.406	1.00
29_V	359_K	2.337	1.00
274_T	303_D	2.322	1.00
251_A	279_R	2.310	1.00
356_F	360_F	2.306	1.00
25_I	371_L	2.299	1.00
363_L	371_L	2.294	1.00
23_I	54_V	2.291	1.00
306_N	350_E	2.235	1.00
115_I	410_V	2.213	1.00
339_D	342_A	2.186	1.00
371_L	404_M	2.182	1.00
216_A	219_A	2.161	1.00
403_V	411_Y	2.160	1.00
342_A	346_T	2.116	1.00
29_V	368_K	2.090	1.00
9_A	21_V	2.078	1.00
116_K	411_Y	2.035	1.00
346_T	362_L	2.018	1.00
52_R	373_I	1.987	1.00
7_V	54_V	1.968	1.00
24_A	44_A	1.948	1.00
52_R	375_D	1.909	1.00
6_N	48_E	1.876	1.00
28_D	406_N	1.863	1.00
375_D	401_H	1.834	1.00
54_V	371_L	1.821	1.00
23_I	363_L	1.819	1.00
13_H	364_E	1.798	1.00
99_D	102_S	1.786	1.00
5_K	22_D	1.781	1.00
409_M	412_E	1.750	1.00
41_Y	44_A	1.729	1.00
55_M	58_I	1.712	1.00
34_G	37_L	1.696	1.00
274_T	278_Q	1.681	1.00
33_I	365_A	1.664	1.00
372_T	400_V	1.647	1.00
18_Q	21_V	1.596	1.00
347_N	350_E	1.595	1.00
49_M	54_V	1.577	1.00
9_A	23_I	1.554	1.00
4_L	25_I	1.527	1.00
342_A	347_N	1.524	1.00
23_I	371_L	1.518	1.00
176_F	179_L	1.509	1.00
30_I	363_L	1.507	1.00
24_A	32_A	1.506	1.00
358_A	367_Y	1.506	1.00
215_E	219_A	1.483	1.00
308_A	351_L	1.474	1.00
273_I	303_D	1.460	1.00
401_H	413_D	1.446	1.00
248_A	252_Q	1.437	1.00
22_D	36_A	1.424	1.00
301_L	339_D	1.400	1.00
300_H	305_R	1.398	1.00
346_T	350_E	1.397	1.00
275_L	279_R	1.395	1.00
402_S	412_E	1.386	1.00
6_N	51_G	1.362	1.00
59_V	348_G	1.353	1.00
206_P	209_G	1.339	1.00
211_E	215_E	1.337	1.00
61_S	308_A	1.337	1.00
207_D	211_E	1.336	1.00
211_E	252_Q	1.319	1.00
373_I	402_S	1.316	1.00
64_H	264_H	1.310	1.00
319_F	322_M	1.309	1.00
218_K	260_T	1.303	1.00
4_L	371_L	1.295	1.00
405_V	410_V	1.294	1.00
114_A	122_V	1.291	1.00
373_I	404_M	1.291	1.00
178_R	182_E	1.290	1.00
282_F	351_L	1.286	1.00
24_A	31_V	1.286	1.00
374_C	400_V	1.283	1.00
350_E	362_L	1.259	1.00
208_A	211_E	1.259	1.00
212_M	215_E	1.257	1.00
12_L	346_T	1.256	1.00
61_S	348_G	1.254	1.00
8_T	18_Q	1.249	1.00
53_I	376_Y	1.248	1.00
341_F	345_L	1.243	1.00
178_R	219_A	1.237	1.00
356_F	359_K	1.235	1.00
216_A	220_T	1.232	1.00
308_A	347_N	1.230	1.00
225_H	263_A	1.230	1.00
4_L	404_M	1.227	1.00
217_V	224_L	1.223	1.00
192_L	195_A	1.220	1.00
275_L	278_Q	1.208	1.00
21_V	33_I	1.196	1.00
22_D	34_G	1.190	1.00
138_T	141_D	1.186	1.00
7_V	373_I	1.183	1.00
171_E	175_R	1.179	1.00
24_A	41_Y	1.176	1.00
320_E	323_K	1.176	1.00
282_F	306_N	1.174	1.00
346_T	367_Y	1.171	1.00
55_M	341_F	1.169	1.00
380_T	399_S	1.166	1.00
22_D	37_L	1.166	1.00
21_V	34_G	1.165	1.00
123_I	352_M	1.163	1.00
45_S	409_M	1.159	1.00
25_I	404_M	1.155	1.00
59_V	121_S	1.153	1.00
47_K	404_M	1.151	1.00
61_S	284_V	1.150	1.00
121_S	360_F	1.138	1.00
276_L	280_D	1.136	1.00
24_A	43_D	1.129	1.00
104_Y	141_D	1.129	1.00
21_V	35_D	1.129	1.00
172_E	176_F	1.127	1.00
31_V	365_A	1.121	1.00
264_H	268_L	1.121	1.00
323_K	327_F	1.118	1.00
262_V	265_G	1.117	1.00
8_T	19_E	1.116	1.00
155_E	158_D	1.106	1.00
173_N	176_F	1.100	1.00
4_L	54_V	1.098	1.00
3_I	22_D	1.096	1.00
284_V	308_A	1.089	1.00
138_T	142_A	1.085	1.00
265_G	268_L	1.081	1.00
276_L	283_L	1.062	1.00
223_G	355_N	1.062	1.00
137_S	141_D	1.061	1.00
271_D	274_T	1.054	1.00
221_G	259_K	1.053	1.00
281_A	284_V	1.052	1.00
177_A	180_I	1.052	1.00
59_V	360_F	1.045	1.00
308_A	348_G	1.042	1.00
47_K	402_S	1.041	1.00
115_I	405_V	1.033	1.00
6_N	20_G	1.027	1.00
13_H	16_K	1.024	1.00
265_G	283_L	1.015	1.00
175_R	178_R	1.014	1.00
6_N	50_H	1.008	1.00
286_N	289_S	1.007	1.00
194_E	199_A	1.004	1.00
61_S	352_M	0.998	1.00
326_F	330_R	0.994	1.00
108_L	112_L	0.993	1.00
30_I	371_L	0.993	1.00
143_F	147_G	0.987	1.00
217_V	222_R	0.984	1.00
322_M	344_A	0.983	1.00
111_S	115_I	0.982	1.00
190_P	194_E	0.980	1.00
179_L	182_E	0.977	1.00
179_L	183_A	0.976	1.00
43_D	46_F	0.975	1.00
108_L	145_K	0.972	1.00
115_I	403_V	0.972	1.00
141_D	145_K	0.971	1.00
293_N	296_G	0.968	1.00
245_D	272_D	0.967	1.00
11_Q	14_P	0.963	1.00
64_H	227_H	0.962	1.00
172_E	175_R	0.955	1.00
194_E	198_G	0.943	1.00
198_G	201_A	0.942	1.00
318_M	324_F	0.937	1.00
277_N	305_R	0.932	1.00
289_S	292_N	0.931	1.00
154_F	158_D	0.931	1.00
319_F	324_F	0.931	1.00
250_L	254_D	0.930	1.00
377_N	380_T	0.929	1.00
59_V	349_N	0.922	1.00
324_F	327_F	0.922	1.00
285_H	289_S	0.921	1.00
7_V	371_L	0.920	1.00
64_H	312_D	0.920	1.00
59_V	352_M	0.915	1.00
335_P	343_K	0.905	1.00
227_H	312_D	0.905	1.00
156_T	159_R	0.902	1.00
8_T	53_I	0.899	1.00
372_T	403_V	0.899	1.00
322_M	326_F	0.897	1.00
264_H	267_Y	0.897	1.00
49_M	52_R	0.895	1.00
24_A	34_G	0.895	1.00
65_F	110_C	0.892	1.00
264_H	269_S	0.883	1.00
4_L	47_K	0.882	1.00
8_T	20_G	0.876	1.00
326_F	329_H	0.876	1.00
115_I	411_Y	0.875	1.00
268_L	300_H	0.874	1.00
359_K	367_Y	0.873	1.00
317_D	324_F	0.873	1.00
343_K	347_N	0.866	1.00
344_A	348_G	0.864	1.00
169_G	172_E	0.862	1.00
10_V	341_F	0.854	1.00
317_D	320_E	0.854	1.00
65_F	122_V	0.852	1.00
248_A	271_D	0.852	1.00
8_T	377_N	0.850	1.00
62_H	264_H	0.849	1.00
321_E	324_F	0.849	1.00
189_E	195_A	0.846	1.00
261_L	351_L	0.840	1.00
192_L	196_H	0.838	1.00
175_R	179_L	0.838	1.00
264_H	312_D	0.833	1.00
318_M	321_E	0.832	1.00
190_P	195_A	0.832	1.00
227_H	264_H	0.831	1.00
286_N	310_G	0.828	1.00
270_K	299_H	0.827	1.00
37_L	41_Y	0.826	1.00
188_S	191_Y	0.816	1.00
151_M	155_E	0.815	1.00
3_I	43_D	0.810	1.00
104_Y	145_K	0.810	1.00
400_V	403_V	0.809	1.00
270_K	274_T	0.807	1.00
285_H	290_N	0.804	1.00
62_H	227_H	0.800	1.00
146_V	149_R	0.800	1.00
13_H	362_L	0.797	1.00
326_F	340_S	0.797	1.00
155_E	159_R	0.796	1.00
58_I	344_A	0.793	1.00
121_S	370_D	0.788	1.00
62_H	312_D	0.787	1.00
269_S	272_D	0.786	1.00
9_A	19_E	0.784	1.00
289_S	293_N	0.780	1.00
375_D	378_S	0.779	1.00
286_N	290_N	0.777	1.00
375_D	399_S	0.771	1.00
65_F	111_S	0.770	1.00
228_A	262_V	0.770	1.00
249_R	253_F	0.768	1.00
194_E	197_I	0.765	1.00
319_F	323_K	0.764	1.00
149_R	153_C	0.762	1.00
5_K	48_E	0.759	1.00
290_N	294_H	0.757	1.00
277_N	303_D	0.756	1.00
189_E	192_L	0.754	1.00
125_H	263_A	0.754	1.00
150_A	154_F	0.752	1.00
10_V	53_I	0.750	1.00
309_L	343_K	0.748	1.00
60_C	311_T	0.746	1.00
276_L	281_A	0.744	1.00
157_T	160_N	0.744	1.00
146_V	151_M	0.744	1.00
22_D	32_A	0.740	1.00
276_L	279_R	0.737	1.00
273_I	277_N	0.734	1.00
6_N	49_M	0.732	1.00
143_F	150_A	0.730	1.00
210_L	226_I	0.730	1.00
284_V	289_S	0.729	1.00
23_I	33_I	0.728	1.00
155_E	160_N	0.727	1.00
306_N	347_N	0.726	1.00
214_R	224_L	0.726	1.00
52_R	402_S	0.723	1.00
271_D	275_L	0.723	1.00
21_V	363_L	0.723	1.00
12_L	341_F	0.720	1.00
144_L	147_G	0.720	1.00
32_A	37_L	0.720	1.00
248_A	251_A	0.718	1.00
18_Q	338_P	0.718	1.00
181_D	222_R	0.717	1.00
125_H	225_H	0.716	1.00
189_E	194_E	0.715	1.00
324_F	328_K	0.715	1.00
309_L	325_A	0.714	1.00
154_F	160_N	0.713	1.00
66_Y	127_A	0.713	1.00
323_K	329_H	0.712	1.00
362_L	365_A	0.711	1.00
21_V	36_A	0.710	1.00
290_N	293_N	0.709	1.00
150_A	153_C	0.704	1.00
284_V	290_N	0.704	1.00
113_E	116_K	0.700	1.00
324_F	329_H	0.700	1.00
349_N	361_G	0.699	1.00
196_H	199_A	0.699	1.00
4_L	373_I	0.695	1.00
195_A	199_A	0.695	1.00
120_T	403_V	0.695	1.00
283_L	300_H	0.690	1.00
273_I	300_H	0.690	1.00
123_I	261_L	0.689	1.00
63_N	114_A	0.686	1.00
176_F	180_I	0.686	1.00
188_S	194_E	0.684	1.00
140_R	146_V	0.683	1.00
153_C	158_D	0.683	1.00
20_G	50_H	0.682	1.00
352_M	356_F	0.681	1.00
17_V	338_P	0.679	1.00
60_C	63_N	0.674	1.00
262_V	276_L	0.672	1.00
10_V	18_Q	0.671	1.00
12_L	362_L	0.671	1.00
122_V	150_A	0.670	1.00
173_N	177_A	0.670	1.00
142_A	145_K	0.669	1.00
274_T	277_N	0.669	1.00
338_P	342_A	0.668	1.00
154_F	157_T	0.667	1.00
299_H	302_S	0.666	1.00
112_L	116_K	0.665	1.00
207_D	252_Q	0.664	1.00
153_C	157_T	0.663	1.00
245_D	248_A	0.662	1.00
178_R	216_A	0.660	1.00
277_N	280_D	0.660	1.00
259_K	350_E	0.659	1.00
5_K	36_A	0.658	1.00
141_D	144_L	0.657	1.00
209_G	212_M	0.657	1.00
58_I	119_C	0.656	1.00
213_L	216_A	0.656	1.00
153_C	156_T	0.655	1.00
323_K	326_F	0.654	1.00
321_E	325_A	0.653	1.00
120_T	370_D	0.653	1.00
140_R	147_G	0.652	1.00
225_H	261_L	0.652	1.00
151_M	157_T	0.650	1.00
10_V	17_V	0.649	1.00
251_A	275_L	0.649	1.00
323_K	328_K	0.645	1.00
250_L	255_L	0.644	1.00
359_K	362_L	0.643	1.00
121_S	352_M	0.643	1.00
191_Y	195_A	0.641	1.00
288_R	292_N	0.639	1.00
177_A	181_D	0.639	1.00
280_D	284_V	0.638	1.00
325_A	328_K	0.637	1.00
191_Y	194_E	0.637	1.00
318_M	325_A	0.636	1.00
121_S	356_F	0.636	1.00
54_V	404_M	0.635	1.00
273_I	276_L	0.635	1.00
233_Y	237_Y	0.635	1.00
337_W	379_P	0.633	1.00
290_N	296_G	0.633	1.00
170_V	173_N	0.630	1.00
170_V	181_D	0.630	1.00
24_A	37_L	0.627	1.00
302_S	339_D	0.627	1.00
139_L	143_F	0.626	1.00
324_F	330_R	0.625	1.00
172_E	177_A	0.625	1.00
104_Y	108_L	0.624	1.00
319_F	374_C	0.623	1.00
229_A	266_L	0.623	1.00
322_M	329_H	0.622	1.00
283_L	305_R	0.622	1.00
321_E	327_F	0.619	1.00
402_S	411_Y	0.618	1.00
317_D	322_M	0.616	1.00
320_E	324_F	0.616	1.00
188_S	193_V	0.615	1.00
142_A	146_V	0.613	1.00
349_N	367_Y	0.613	1.00
230_E	233_Y	0.611	1.00
145_K	148_L	0.611	1.00
285_H	288_R	0.610	1.00
22_D	38_T	0.610	1.00
156_T	160_N	0.609	1.00
33_I	363_L	0.607	1.00
272_D	275_L	0.607	1.00
20_G	33_I	0.605	1.00
139_L	142_A	0.604	1.00
182_E	220_T	0.604	1.00
317_D	321_E	0.600	1.00
263_A	266_L	0.597	1.00
292_N	296_G	0.595	1.00
131_Y	134_G	0.594	1.00
140_R	144_L	0.593	1.00
20_G	36_A	0.593	1.00
100_E	104_Y	0.593	1.00
171_E	177_A	0.592	1.00
214_R	218_K	0.592	1.00
318_M	323_K	0.590	1.00
317_D	323_K	0.590	1.00
139_L	144_L	0.590	1.00
166_L	169_G	0.589	1.00
17_V	20_G	0.589	1.00
109_I	113_E	0.585	1.00
140_R	143_F	0.585	1.00
318_M	328_K	0.584	1.00
319_F	326_F	0.584	1.00
58_I	400_V	0.584	1.00
147_G	153_C	0.584	1.00
263_A	284_V	0.584	1.00
171_E	174_I	0.583	1.00
12_L	342_A	0.582	1.00
294_H	297_Y	0.582	1.00
261_L	284_V	0.581	1.00
290_N	295_V	0.580	1.00
322_M	330_R	0.579	1.00
148_L	151_M	0.579	1.00
208_A	212_M	0.579	1.00
325_A	330_R	0.578	1.00
30_I	367_Y	0.578	1.00
247_L	251_A	0.575	1.00
174_I	177_A	0.575	1.00
137_S	140_R	0.575	1.00
109_I	112_L	0.573	1.00
143_F	146_V	0.570	1.00
23_I	30_I	0.569	1.00
282_F	308_A	0.568	1.00
34_G	41_Y	0.567	1.00
251_A	278_Q	0.566	1.00
319_F	325_A	0.566	1.00
190_P	193_V	0.565	1.00
360_F	370_D	0.565	1.00
154_F	159_R	0.565	1.00
58_I	319_F	0.565	1.00
406_N	409_M	0.563	1.00
180_I	184_K	0.563	1.00
319_F	328_K	0.563	1.00
211_E	253_F	0.562	1.00
335_P	339_D	0.561	1.00
284_V	288_R	0.561	1.00
138_T	143_F	0.560	1.00
214_R	255_L	0.559	1.00
149_R	360_F	0.558	1.00
173_N	178_R	0.557	1.00
49_M	402_S	0.556	1.00
150_A	158_D	0.555	1.00
181_D	184_K	0.555	1.00
270_K	303_D	0.554	1.00
263_A	268_L	0.552	1.00
339_D	343_K	0.552	1.00
322_M	325_A	0.552	1.00
193_V	196_H	0.551	1.00
151_M	154_F	0.550	1.00
111_S	122_V	0.546	1.00
286_N	292_N	0.546	1.00
102_S	105_Y	0.544	1.00
229_A	286_N	0.543	1.00
403_V	409_M	0.543	1.00
370_D	406_N	0.543	1.00
12_L	338_P	0.541	1.00
318_M	327_F	0.540	1.00
360_F	367_Y	0.538	1.00
246_L	250_L	0.535	1.00
3_I	41_Y	0.533	1.00
181_D	220_T	0.533	1.00
180_I	183_A	0.533	1.00
247_L	272_D	0.533	1.00
53_I	374_C	0.531	1.00
47_K	409_M	0.529	1.00
209_G	213_L	0.529	1.00
11_Q	18_Q	0.528	1.00
39_Q	44_A	0.526	1.00
20_G	35_D	0.526	1.00
248_A	275_L	0.526	1.00
119_C	318_M	0.526	1.00
348_G	351_L	0.526	1.00
193_V	197_I	0.521	1.00
53_I	337_W	0.521	1.00
320_E	327_F	0.521	1.00
121_S	151_M	0.521	1.00
293_N	297_Y	0.520	1.00
7_V	51_G	0.518	1.00
207_D	249_R	0.518	1.00
318_M	322_M	0.518	1.00
168_E	172_E	0.515	1.00
371_L	409_M	0.515	1.00
193_V	198_G	0.514	1.00
173_N	180_I	0.513	1.00
270_K	273_I	0.513	1.00
25_I	31_V	0.513	1.00
170_V	177_A	0.512	1.00
378_S	395_M	0.512	1.00
173_N	181_D	0.512	1.00
283_L	290_N	0.511	1.00
27_N	31_V	0.511	1.00
236_S	272_D	0.509	1.00
150_A	157_T	0.509	1.00
190_P	196_H	0.509	1.00
139_L	146_V	0.509	1.00
212_M	216_A	0.509	1.00
100_E	141_D	0.508	1.00
192_L	200_H	0.508	1.00
32_A	38_T	0.507	1.00
171_E	212_M	0.507	1.00
232_L	236_S	0.506	1.00
265_G	273_I	0.505	1.00
318_M	326_F	0.502	1.00
259_K	355_N	0.501	1.00
190_P	199_A	0.501	1.00
58_I	341_F	0.500	1.00
365_A	368_K	0.500	1.00
248_A	269_S	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4dzhA	2	0.9457	100	0.266	Contact Map
3lnpA	2	0.948	100	0.268	Contact Map
3ls9A	2	0.9615	100	0.27	Contact Map
2pajA	2	0.8982	100	0.276	Contact Map
4v1xA	5	0.9729	100	0.288	Contact Map
3hpaA	2	0.9344	100	0.291	Contact Map
4gbdA	2	0.9434	100	0.293	Contact Map
4f0rA	2	0.948	100	0.305	Contact Map
2i9uA	2	0.8959	100	0.313	Contact Map
4f0lA	2	0.9412	100	0.318	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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