ARNC - ECOLI - GREMLIN Contacts

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ARNC - Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase

UniProt:	P77757	Sequences: MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEILLIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDADLQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYGCMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLINLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAVLFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG14090
Length:	322 (277)
Sequences:	16801 (13439)
Seq/√Len:	807.4

ECOLI - ARNC GTRB PGAC YAIP YIBD WCAA WCAE WBBL
BACSU - CSBB YKCC YKOT YDAM GGAA EPSH TUAG CGED EPSE EPSJ GGAB SPSA YFNE
HALSA - Q9HQQ6 Q9HSB1 Q9HQP4 Q9HR08 Q9HQP8 Q9HQP3 Q9HNK3 Q9HSC8
SULSO - Q97TY0 Q97W52 Q97WK7 Q7LXY2 Q97UK7 Q97YM4 Q97TZ0 Q7LXF7 Q97X05 Q97YG5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
20_Q	53_N	4.849	1.00
47_D	73_L	4.411	1.00
168_R	172_D	4.215	1.00
53_N	56_H	3.251	1.00
11_S	93_G	3.111	1.00
11_S	42_E	3.024	1.00
121_Y	196_I	3.003	1.00
58_L	71_S	2.911	1.00
95_L	117_A	2.834	1.00
42_E	70_V	2.670	1.00
9_K	94_D	2.628	1.00
16_V	54_S	2.624	1.00
116_K	119_E	2.618	1.00
55_A	73_L	2.594	1.00
47_D	50_S	2.577	1.00
46_I	87_G	2.514	1.00
55_A	59_V	2.456	1.00
24_P	60_E	2.439	1.00
97_I	165_A	2.315	1.00
24_P	57_M	2.277	1.00
170_I	193_R	2.266	1.00
19_E	101_A	2.226	1.00
116_K	196_I	2.211	1.00
24_P	28_R	2.182	1.00
55_A	71_S	2.146	1.00
95_L	165_A	2.093	1.00
45_L	58_L	2.088	1.00
117_A	165_A	2.035	1.00
80_Q	84_I	2.021	1.00
74_L	86_A	2.017	1.00
9_K	40_E	2.005	1.00
27_I	61_A	1.984	1.00
56_H	60_E	1.972	1.00
28_R	61_A	1.967	1.00
112_R	200_V	1.966	1.00
57_M	60_E	1.942	1.00
10_V	114_V	1.925	1.00
11_S	44_L	1.923	1.00
183_F	187_L	1.912	1.00
122_D	167_R	1.880	1.00
9_K	92_T	1.879	1.00
11_S	91_V	1.862	1.00
18_N	51_S	1.852	1.00
116_K	198_I	1.844	1.00
112_R	115_A	1.817	1.00
95_L	114_V	1.800	1.00
53_N	57_M	1.787	1.00
122_D	193_R	1.784	1.00
173_A	193_R	1.784	1.00
59_V	73_L	1.775	1.00
37_L	111_P	1.717	1.00
173_A	191_F	1.715	1.00
113_L	165_A	1.706	1.00
13_V	87_G	1.702	1.00
97_I	110_I	1.679	1.00
42_E	93_G	1.665	1.00
18_N	50_S	1.665	1.00
129_Q	201_H	1.588	1.00
84_I	98_T	1.588	1.00
117_A	167_R	1.575	1.00
88_F	171_V	1.560	1.00
17_Y	77_N	1.545	1.00
86_A	90_H	1.542	1.00
88_F	96_I	1.538	1.00
28_R	32_T	1.503	1.00
37_L	40_E	1.497	1.00
9_K	93_G	1.476	1.00
13_V	46_I	1.472	1.00
25_E	28_R	1.446	1.00
42_E	92_T	1.407	1.00
72_I	90_H	1.405	1.00
149_Q	152_T	1.403	1.00
108_E	112_R	1.401	1.00
16_V	101_A	1.380	1.00
169_H	173_A	1.378	1.00
29_R	105_N	1.369	1.00
46_I	83_A	1.367	1.00
14_I	26_L	1.364	1.00
134_S	137_R	1.361	1.00
47_D	55_A	1.350	1.00
10_V	110_I	1.349	1.00
123_V	195_A	1.345	1.00
29_R	108_E	1.339	1.00
96_I	171_V	1.307	1.00
20_Q	57_M	1.292	1.00
123_V	188_A	1.278	1.00
27_I	45_L	1.261	1.00
91_V	168_R	1.240	1.00
216_R	220_L	1.228	1.00
127_V	199_P	1.218	1.00
25_E	206_E	1.208	1.00
179_E	182_T	1.194	1.00
31_T	66_N	1.192	1.00
56_H	59_V	1.187	1.00
27_I	66_N	1.180	1.00
105_N	109_E	1.179	1.00
115_A	118_D	1.174	1.00
99_L	105_N	1.164	1.00
128_R	162_M	1.153	1.00
80_Q	100_D	1.144	1.00
48_D	77_N	1.128	1.00
60_E	65_E	1.123	1.00
135_W	138_K	1.121	1.00
48_D	79_G	1.112	1.00
115_A	119_E	1.111	1.00
278_F	282_G	1.102	1.00
70_V	90_H	1.102	1.00
96_I	168_R	1.099	1.00
113_L	126_T	1.098	1.00
16_V	45_L	1.097	1.00
44_L	70_V	1.088	1.00
89_S	175_L	1.083	1.00
146_R	150_R	1.080	1.00
86_A	89_S	1.079	1.00
41_Y	68_H	1.076	1.00
29_R	104_Q	1.074	1.00
146_R	149_Q	1.074	1.00
210_S	213_S	1.070	1.00
25_E	29_R	1.064	1.00
135_W	139_T	1.061	1.00
23_L	57_M	1.057	1.00
105_N	202_H	1.057	1.00
167_R	170_I	1.052	1.00
170_I	188_A	1.044	1.00
16_V	19_E	1.039	1.00
12_V	110_I	1.037	1.00
14_I	101_A	1.034	1.00
116_K	124_V	1.028	1.00
223_D	226_T	1.021	1.00
25_E	104_Q	1.012	1.00
95_L	167_R	1.006	1.00
57_M	61_A	1.003	1.00
12_V	97_I	0.997	1.00
109_E	163_L	0.995	1.00
137_R	140_A	0.990	1.00
169_H	172_D	0.984	1.00
87_G	91_V	0.984	1.00
24_P	61_A	0.980	1.00
216_R	219_N	0.975	1.00
60_E	63_Q	0.970	1.00
126_T	163_L	0.969	1.00
27_I	31_T	0.965	1.00
88_F	98_T	0.964	1.00
173_A	176_H	0.946	1.00
282_G	286_I	0.942	1.00
240_G	244_A	0.942	1.00
217_L	220_L	0.940	1.00
8_K	118_D	0.936	1.00
26_L	30_T	0.926	1.00
44_L	91_V	0.916	1.00
14_I	43_I	0.912	1.00
62_S	69_I	0.907	1.00
72_I	86_A	0.899	1.00
116_K	120_G	0.899	1.00
85_M	89_S	0.894	1.00
159_Y	164_R	0.893	1.00
280_F	284_Q	0.892	1.00
33_A	111_P	0.884	1.00
31_T	68_H	0.883	1.00
149_Q	153_G	0.878	1.00
70_V	92_T	0.874	1.00
143_M	146_R	0.871	1.00
29_R	33_A	0.870	1.00
234_R	238_L	0.870	1.00
244_A	247_G	0.866	1.00
22_S	206_E	0.866	1.00
9_K	42_E	0.864	1.00
276_V	280_F	0.857	1.00
198_I	201_H	0.855	1.00
94_D	169_H	0.852	1.00
32_T	107_P	0.852	1.00
14_I	30_T	0.850	1.00
166_Y	188_A	0.847	1.00
14_I	45_L	0.846	1.00
263_P	266_A	0.844	1.00
18_N	52_D	0.842	1.00
23_L	54_S	0.841	1.00
27_I	69_I	0.840	1.00
205_R	208_G	0.835	1.00
240_G	247_G	0.834	1.00
59_V	62_S	0.829	1.00
28_R	65_E	0.822	1.00
19_E	22_S	0.820	1.00
80_Q	161_C	0.819	1.00
26_L	29_R	0.814	1.00
12_V	30_T	0.812	1.00
166_Y	171_V	0.811	1.00
29_R	203_A	0.810	1.00
268_E	271_F	0.803	1.00
84_I	100_D	0.802	1.00
97_I	109_E	0.798	1.00
237_S	240_G	0.797	1.00
103_L	205_R	0.794	1.00
16_V	23_L	0.790	1.00
109_E	112_R	0.787	1.00
117_A	124_V	0.786	1.00
145_N	149_Q	0.786	1.00
45_L	69_I	0.784	1.00
139_T	143_M	0.779	1.00
17_Y	51_S	0.778	1.00
188_A	193_R	0.774	1.00
11_S	168_R	0.774	1.00
112_R	116_K	0.772	1.00
20_Q	52_D	0.772	1.00
136_F	140_A	0.770	1.00
136_F	139_T	0.769	1.00
220_L	223_D	0.768	1.00
29_R	32_T	0.768	1.00
167_R	193_R	0.768	1.00
137_R	141_S	0.767	1.00
111_P	115_A	0.765	1.00
8_K	114_V	0.759	1.00
58_L	62_S	0.758	1.00
59_V	63_Q	0.756	1.00
138_K	142_K	0.756	1.00
41_Y	110_I	0.756	1.00
91_V	96_I	0.753	1.00
269_G	272_M	0.750	1.00
138_K	141_S	0.748	1.00
127_V	197_E	0.748	1.00
30_T	43_I	0.746	1.00
279_T	283_A	0.744	1.00
247_G	251_A	0.742	1.00
113_L	201_H	0.741	1.00
23_L	45_L	0.736	1.00
14_I	99_L	0.734	1.00
78_Y	82_S	0.734	1.00
273_L	276_V	0.733	1.00
15_P	83_A	0.732	1.00
38_G	41_Y	0.730	1.00
228_L	231_T	0.722	1.00
75_N	86_A	0.719	1.00
22_S	26_L	0.718	1.00
196_I	199_P	0.715	1.00
23_L	58_L	0.715	1.00
241_S	244_A	0.715	1.00
18_N	54_S	0.715	1.00
139_T	142_K	0.713	1.00
247_G	279_T	0.712	1.00
94_D	167_R	0.711	1.00
222_Y	226_T	0.711	1.00
148_I	152_T	0.710	1.00
44_L	87_G	0.709	1.00
282_G	285_F	0.707	1.00
81_H	85_M	0.700	1.00
50_S	54_S	0.698	1.00
263_P	268_E	0.696	1.00
266_A	271_F	0.696	1.00
122_D	169_H	0.695	1.00
31_T	61_A	0.694	1.00
81_H	180_R	0.693	1.00
146_R	152_T	0.693	1.00
270_V	274_F	0.691	1.00
37_L	41_Y	0.690	1.00
88_F	91_V	0.683	1.00
147_L	152_T	0.682	1.00
179_E	223_D	0.682	1.00
144_I	149_Q	0.681	1.00
93_G	168_R	0.680	1.00
221_M	224_L	0.680	1.00
129_Q	199_P	0.677	1.00
174_M	188_A	0.677	1.00
281_I	285_F	0.676	1.00
232_P	235_M	0.674	1.00
42_E	91_V	0.672	1.00
249_S	253_L	0.666	1.00
121_Y	194_R	0.664	1.00
112_R	198_I	0.664	1.00
144_I	148_I	0.660	1.00
43_I	69_I	0.659	1.00
258_R	262_G	0.658	1.00
13_V	91_V	0.657	1.00
124_V	198_I	0.655	1.00
241_S	245_I	0.653	1.00
75_N	78_Y	0.651	1.00
23_L	28_R	0.650	1.00
143_M	147_L	0.650	1.00
79_G	102_D	0.650	1.00
39_K	118_D	0.648	1.00
49_G	77_N	0.646	1.00
116_K	121_Y	0.646	1.00
18_N	47_D	0.645	1.00
17_Y	48_D	0.643	1.00
23_L	101_A	0.643	1.00
216_R	223_D	0.643	1.00
48_D	100_D	0.641	1.00
220_L	224_L	0.641	1.00
62_S	71_S	0.640	1.00
113_L	117_A	0.640	1.00
114_V	165_A	0.639	1.00
272_M	276_V	0.638	1.00
178_H	187_L	0.638	1.00
114_V	118_D	0.637	1.00
144_I	147_L	0.636	1.00
239_L	244_A	0.635	1.00
98_T	164_R	0.634	1.00
256_I	260_T	0.631	1.00
113_L	163_L	0.631	1.00
232_P	240_G	0.630	1.00
19_E	54_S	0.629	1.00
103_L	203_A	0.626	1.00
62_S	66_N	0.625	1.00
24_P	65_E	0.624	1.00
33_A	110_I	0.624	1.00
170_I	173_A	0.624	1.00
122_D	194_R	0.621	1.00
238_L	242_I	0.620	1.00
214_F	217_L	0.620	1.00
140_A	144_I	0.620	1.00
214_F	218_I	0.619	1.00
223_D	227_C	0.616	1.00
279_T	282_G	0.615	1.00
184_I	188_A	0.614	1.00
26_L	101_A	0.611	1.00
11_S	92_T	0.611	1.00
234_R	237_S	0.610	1.00
105_N	163_L	0.610	1.00
284_Q	288_M	0.610	1.00
8_K	94_D	0.610	1.00
125_G	128_R	0.609	1.00
278_F	281_I	0.609	1.00
15_P	48_D	0.607	1.00
94_D	168_R	0.606	1.00
183_F	186_I	0.602	1.00
239_L	243_I	0.600	1.00
248_F	255_V	0.600	1.00
66_N	69_I	0.599	1.00
125_G	159_Y	0.599	1.00
278_F	285_F	0.596	1.00
140_A	143_M	0.595	1.00
44_L	90_H	0.589	1.00
30_T	105_N	0.585	1.00
218_I	222_Y	0.584	1.00
215_M	218_I	0.583	1.00
54_S	58_L	0.582	1.00
219_N	222_Y	0.578	1.00
280_F	285_F	0.576	1.00
48_D	102_D	0.575	1.00
137_R	142_K	0.574	1.00
58_L	61_A	0.572	1.00
109_E	113_L	0.571	1.00
38_G	114_V	0.570	1.00
236_L	287_G	0.568	1.00
17_Y	49_G	0.566	1.00
55_A	58_L	0.564	1.00
224_L	227_C	0.563	1.00
254_L	257_L	0.559	1.00
267_A	270_V	0.558	1.00
15_P	101_A	0.556	1.00
135_W	140_A	0.556	1.00
108_E	111_P	0.556	1.00
30_T	99_L	0.556	1.00
285_F	289_G	0.555	1.00
19_E	80_Q	0.555	1.00
245_I	249_S	0.555	1.00
125_G	162_M	0.554	1.00
253_L	257_L	0.554	1.00
113_L	116_K	0.553	1.00
216_R	221_M	0.553	1.00
20_Q	56_H	0.552	1.00
189_N	195_A	0.552	1.00
283_A	287_G	0.550	1.00
9_K	41_Y	0.549	1.00
270_V	273_L	0.548	1.00
49_G	74_L	0.547	1.00
31_T	69_I	0.546	1.00
215_M	219_N	0.543	1.00
281_I	284_Q	0.542	1.00
252_V	256_I	0.540	1.00
219_N	223_D	0.539	1.00
74_L	78_Y	0.536	1.00
121_Y	124_V	0.533	1.00
54_S	101_A	0.532	1.00
185_P	188_A	0.531	1.00
178_H	183_F	0.530	1.00
251_A	275_A	0.530	1.00
250_I	254_L	0.530	1.00
26_L	31_T	0.528	1.00
80_Q	98_T	0.527	1.00
244_A	248_F	0.526	1.00
111_P	114_V	0.526	1.00
277_L	280_F	0.526	1.00
217_L	221_M	0.525	1.00
257_L	261_F	0.524	1.00
80_Q	180_R	0.523	1.00
17_Y	47_D	0.523	1.00
33_A	37_L	0.522	1.00
128_R	158_D	0.521	1.00
147_L	151_T	0.520	1.00
16_V	58_L	0.519	1.00
22_S	101_A	0.519	1.00
142_K	146_R	0.517	1.00
26_L	99_L	0.516	1.00
85_M	178_H	0.516	1.00
170_I	174_M	0.514	1.00
15_P	84_I	0.513	1.00
237_S	241_S	0.513	1.00
233_L	237_S	0.512	1.00
59_V	71_S	0.512	1.00
23_L	61_A	0.510	1.00
106_P	109_E	0.509	1.00
97_I	113_L	0.509	1.00
145_N	148_I	0.508	1.00
275_A	279_T	0.507	1.00
271_F	286_I	0.507	1.00
29_R	103_L	0.506	1.00
218_I	221_M	0.506	1.00
27_I	30_T	0.505	1.00
103_L	162_M	0.504	1.00
26_L	103_L	0.504	1.00
286_I	289_G	0.504	1.00
48_D	74_L	0.503	1.00
208_G	211_K	0.503	1.00
251_A	254_L	0.502	1.00
19_E	100_D	0.501	1.00
104_Q	131_R	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4p02A	1	0.972	100	0.401	Contact Map
2z86A	2	0.7671	100	0.451	Contact Map
3bcvA	2	0.5404	100	0.461	Contact Map
2d7iA	1	0.9689	100	0.462	Contact Map
1qg8A	1	0.6646	100	0.467	Contact Map
3l7iA	4	0.0652	100	0.472	Contact Map
1xhbA	1	0.9379	100	0.476	Contact Map
5ajoA	1	0.941	100	0.482	Contact Map
2bo4A	4	0.9037	100	0.485	Contact Map
3ckjA	2	0.795	100	0.492	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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