GREMLIN Database
HYFI - Hydrogenase-4 component I
UniProt: P77668 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG14217
Length: 252 (204)
Sequences: 241 (86)
Seq/√Len: 6.0

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
128_S134_I3.3940.86
32_R71_S2.9800.78
18_Q64_F2.9790.78
17_E25_H2.9790.78
12_P18_Q2.6960.70
17_E122_Y2.6410.68
27_L136_V2.5890.67
45_N122_Y2.5440.65
200_G203_Q2.4220.61
45_N124_V2.2810.56
103_K118_F2.2560.55
91_P144_T2.2350.55
22_M79_L2.2240.54
150_H154_V2.2130.54
122_Y161_Q2.0720.49
11_Q18_Q2.0690.49
75_A118_F2.0600.48
24_R211_L1.9990.46
80_F128_S1.9700.45
15_L174_V1.9350.44
18_Q28_Q1.9050.43
57_P151_G1.8960.43
110_A120_D1.8590.41
91_P94_R1.8420.41
18_Q177_Q1.8310.40
62_E67_K1.8180.40
80_F92_A1.7580.38
92_A132_V1.7390.37
14_T18_Q1.7270.37
84_V137_W1.6520.34
59_F120_D1.6310.34
122_Y183_I1.6020.33
42_G52_F1.5970.33
48_E52_F1.5860.32
185_P206_E1.5830.32
49_I118_F1.5750.32
33_S67_K1.5660.32
84_V132_V1.5160.30
33_S56_T1.5080.30
19_T23_K1.5070.30
53_A64_F1.4970.30
45_N161_Q1.4640.29
187_Q190_A1.4620.29
37_Y62_E1.4520.28
72_P76_D1.4460.28
102_H137_W1.4390.28
25_H122_Y1.4090.27
64_F124_V1.3990.27
72_P127_G1.3850.26
61_A66_I1.3750.26
17_E124_V1.3740.26
112_G131_I1.3710.26
99_A146_A1.3580.26
35_Y159_L1.3530.26
58_V160_Q1.3510.25
59_F110_A1.3350.25
11_Q183_I1.3320.25
28_Q64_F1.3300.25
11_Q15_L1.3290.25
96_Y105_C1.3170.25
84_V88_M1.3100.24
17_E45_N1.2940.24
78_L94_R1.2880.24
91_P161_Q1.2770.24
69_V79_L1.2760.23
166_V179_L1.2680.23
193_R204_G1.2680.23
192_E203_Q1.2570.23
11_Q17_E1.2540.23
42_G55_I1.2360.22
188_R207_I1.2340.22
47_C109_G1.2320.22
105_C114_G1.2290.22
167_D206_E1.2190.22
11_Q16_D1.2100.22
93_L97_E1.2070.22
18_Q24_R1.1980.22
95_A104_I1.1710.21
124_V161_Q1.1640.21
202_R206_E1.1630.21
200_G204_G1.1560.21
22_M34_A1.1540.21
176_M182_Q1.1520.20
37_Y90_M1.1450.20
146_A155_A1.1410.20
90_M132_V1.1130.20
107_S176_M1.1060.19
203_Q206_E1.1050.19
49_I148_T1.0960.19
37_Y87_A1.0940.19
12_P15_L1.0860.19
76_D127_G1.0800.19
22_M31_R1.0710.19
92_A128_S1.0640.18
11_Q64_F1.0610.18
206_E210_R1.0550.18
76_D96_Y1.0530.18
103_K112_G1.0470.18
168_Y174_V1.0450.18
166_V206_E1.0290.18
132_V144_T1.0290.18
37_Y89_R1.0220.18
47_C133_P1.0200.18
17_E20_Q1.0090.17
16_D173_G1.0090.17
71_S193_R1.0090.17
44_C104_I1.0070.17
44_C51_I1.0060.17
68_V114_G1.0060.17
43_G108_Y1.0050.17
93_L112_G0.9960.17
52_F78_L0.9920.17
14_T17_E0.9910.17
15_L28_Q0.9820.17
19_T185_P0.9700.17
107_S184_P0.9690.17
19_T136_V0.9670.16
12_P64_F0.9620.16
14_T197_R0.9600.16
24_R27_L0.9600.16
47_C142_P0.9560.16
205_R209_D0.9540.16
84_V122_Y0.9460.16
109_G133_P0.9430.16
76_D129_D0.9420.16
12_P28_Q0.9400.16
49_I75_A0.9380.16
11_Q179_L0.9330.16
56_T62_E0.9320.16
25_H84_V0.9320.16
190_A194_E0.9260.16
73_R143_P0.9160.16
32_R72_P0.9160.16
55_I114_G0.9160.16
27_L179_L0.9140.15
135_D158_L0.9100.15
92_A134_I0.9090.15
110_A118_F0.9030.15
159_L193_R0.9000.15
43_G76_D0.8970.15
75_A127_G0.8950.15
18_Q144_T0.8910.15
24_R170_D0.8880.15
171_P197_R0.8860.15
171_P187_Q0.8810.15
109_G142_P0.8800.15
49_I55_I0.8800.15
41_C52_F0.8780.15
51_I148_T0.8720.15
18_Q21_K0.8700.15
46_A98_S0.8690.15
12_P195_A0.8570.14
68_V207_I0.8540.14
22_M175_T0.8530.14
30_I74_H0.8500.14
179_L182_Q0.8460.14
166_V170_D0.8420.14
172_T206_E0.8410.14
172_T186_S0.8370.14
178_P203_Q0.8350.14
171_P175_T0.8350.14
37_Y122_Y0.8350.14
166_V213_R0.8290.14
42_G67_K0.8240.14
197_R207_I0.8240.14
62_E66_I0.7960.13
52_F67_K0.7950.13
85_T124_V0.7930.13
56_T149_I0.7920.13
80_F93_L0.7870.13
18_Q53_A0.7840.13
172_T175_T0.7800.13
15_L18_Q0.7790.13
58_V162_K0.7740.13
35_Y52_F0.7720.13
96_Y160_Q0.7720.13
17_E183_I0.7640.13
103_K110_A0.7630.13
11_Q177_Q0.7600.13
192_E195_A0.7590.13
16_D189_I0.7580.13
149_I189_I0.7570.13
188_R193_R0.7550.13
34_A144_T0.7480.13
132_V177_Q0.7480.13
54_A62_E0.7470.13
15_L53_A0.7470.13
185_P215_L0.7440.13
91_P138_I0.7430.13
41_C124_V0.7420.13
64_F195_A0.7370.12
170_D173_G0.7340.12
15_L185_P0.7290.12
204_G207_I0.7290.12
73_R142_P0.7270.12
146_A154_V0.7250.12
25_H113_V0.7240.12
15_L184_P0.7200.12
17_E173_G0.7170.12
80_F144_T0.7150.12
211_L215_L0.7090.12
31_R46_A0.7070.12
38_R146_A0.7050.12
191_I194_E0.6960.12
173_G206_E0.6930.12
78_L148_T0.6920.12
122_Y163_I0.6900.12
39_V67_K0.6880.12
149_I203_Q0.6870.12
35_Y99_A0.6840.12
43_G127_G0.6820.12
24_R169_R0.6770.12
11_Q195_A0.6730.11
36_V79_L0.6720.11
67_K138_I0.6710.11
54_A61_A0.6690.11
112_G134_I0.6680.11
44_C126_G0.6670.11
64_F91_P0.6670.11
110_A130_T0.6640.11
14_T171_P0.6580.11
133_P142_P0.6580.11
46_A87_A0.6560.11
186_S203_Q0.6490.11
13_I171_P0.6450.11
190_A195_A0.6440.11
206_E211_L0.6440.11
41_C94_R0.6380.11
38_R125_W0.6370.11
121_L155_A0.6350.11
41_C88_M0.6340.11
90_M134_I0.6330.11
31_R156_L0.6330.11
106_V147_A0.6320.11
152_F196_R0.6260.11
54_A57_P0.6260.11
192_E206_E0.6240.11
64_F177_Q0.6240.11
46_A74_H0.6230.11
88_M103_K0.6220.11
62_E100_P0.6200.11
62_E138_I0.6140.11
32_R127_G0.6130.11
166_V193_R0.6110.11
189_I199_A0.6070.11
15_L64_F0.6060.11
19_T169_R0.6040.11
174_V183_I0.6010.10
204_G215_L0.6000.10
49_I53_A0.5990.10
30_I46_A0.5920.10
24_R164_H0.5920.10
91_P112_G0.5910.10
118_F145_P0.5910.10
189_I196_R0.5900.10
179_L212_L0.5890.10
120_D145_P0.5880.10
19_T206_E0.5880.10
57_P117_I0.5870.10
39_V83_A0.5870.10
51_I104_I0.5860.10
43_G87_A0.5850.10
97_E110_A0.5840.10
47_C127_G0.5840.10
16_D21_K0.5820.10
84_V205_R0.5790.10
102_H134_I0.5740.10
87_A125_W0.5730.10
45_N91_P0.5680.10
84_V130_T0.5640.10
49_I127_G0.5630.10
88_M94_R0.5600.10
196_R199_A0.5570.10
23_K200_G0.5570.10
187_Q210_R0.5550.10
210_R213_R0.5520.10
30_I155_A0.5520.10
190_A207_I0.5440.10
94_R161_Q0.5420.10
15_L213_R0.5390.10
18_Q164_H0.5370.10
35_Y126_G0.5360.10
17_E165_A0.5300.10
19_T28_Q0.5300.10
195_A200_G0.5290.10
121_L147_A0.5290.10
149_I166_V0.5260.10
81_T108_Y0.5230.09
149_I161_Q0.5230.09
107_S131_I0.5210.09
190_A213_R0.5200.09
161_Q204_G0.5190.09
79_L172_T0.5180.09
11_Q14_T0.5180.09
29_D105_C0.5150.09
52_F55_I0.5140.09
32_R88_M0.5100.09
17_E163_I0.5090.09
38_R108_Y0.5070.09
109_G127_G0.5060.09
171_P205_R0.5060.09
122_Y165_A0.5050.09
15_L190_A0.5050.09
94_R103_K0.5030.09
44_C113_V0.5000.09
41_C122_Y0.5000.09
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3i9v6 1 0.5754 100 0.508 Contact Map
1h2aS 1 0.8135 100 0.657 Contact Map
2wpnA 1 0.8373 100 0.663 Contact Map
4ue3S 2 0.7262 100 0.663 Contact Map
3ayxB 2 0.7817 100 0.664 Contact Map
4uqlA 1 0.8016 100 0.67 Contact Map
4kn9S 1 0.8294 100 0.676 Contact Map
3myrA 3 0.7698 100 0.677 Contact Map
4u9hS 1 0.754 100 0.678 Contact Map
4iucS 1 0.7302 100 0.678 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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