GREMLIN Database
MHPD - 2-keto-4-pentenoate hydratase
UniProt: P77608 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG14274
Length: 269 (255)
Sequences: 2481 (1733)
Seq/√Len: 108.5

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
115_D223_R4.5291.00
54_R85_D3.9761.00
188_N243_D3.7621.00
12_D29_L3.6411.00
34_N37_A3.6251.00
11_A48_H3.5561.00
113_N131_E3.4141.00
109_A136_A3.0291.00
44_I47_Q2.9661.00
66_P70_Q2.8511.00
112_L222_L2.8441.00
115_D224_T2.8051.00
87_C164_Y2.4881.00
110_L230_T2.4691.00
15_R19_Q2.4101.00
99_V163_V2.3331.00
57_V164_Y2.2151.00
115_D221_P2.1391.00
223_R226_D2.1371.00
11_A15_R2.1321.00
46_V83_F2.1051.00
54_R84_A2.0511.00
187_R245_F2.0471.00
24_A145_R2.0461.00
182_A194_S2.0201.00
50_V56_V1.9991.00
116_L129_A1.9421.00
104_I137_L1.9391.00
130_I208_A1.9011.00
191_E194_S1.8661.00
114_R128_N1.8621.00
186_T248_H1.8391.00
106_A247_A1.7601.00
122_T125_E1.7571.00
43_H220_E1.7541.00
246_E256_A1.7361.00
184_K191_E1.7161.00
93_I258_T1.7101.00
49_D54_R1.6681.00
14_R52_Q1.6271.00
17_A49_D1.6011.00
248_H254_S1.5901.00
14_R48_H1.5881.00
111_V132_W1.5861.00
239_V259_F1.5751.00
36_E218_L1.5671.00
182_A196_R1.5551.00
246_E254_S1.5511.00
184_K194_S1.5251.00
111_V134_L1.5171.00
88_Y163_V1.5101.00
57_V87_C1.4941.00
55_R84_A1.4851.00
95_P98_R1.4851.00
90_D168_G1.4741.00
14_R45_N1.4721.00
239_V245_F1.4701.00
184_K248_H1.4021.00
7_E44_I1.4011.00
186_T191_E1.3971.00
119_T213_R1.3881.00
6_L44_I1.3831.00
120_D213_R1.3721.00
126_L209_V1.3621.00
96_F241_A1.3401.00
56_V81_T1.3391.00
12_D23_I1.3381.00
186_T246_E1.3260.99
117_P221_P1.3200.99
205_L209_V1.2840.99
123_F209_V1.2830.99
125_E128_N1.2820.99
110_L208_A1.2810.99
143_R157_D1.2800.99
9_L12_D1.2740.99
123_F206_N1.2730.99
244_R256_A1.2680.99
49_D84_A1.2600.99
89_G92_E1.2600.99
18_E52_Q1.2580.99
244_R258_T1.2540.99
35_A155_V1.2430.99
100_L141_G1.2380.99
88_Y95_P1.2380.99
43_H218_L1.2360.99
187_R243_D1.2350.99
99_V141_G1.2320.99
78_D154_T1.2240.99
109_A227_I1.2230.99
185_M247_A1.2220.99
214_K217_S1.2080.99
88_Y92_E1.2040.99
180_N196_R1.1890.99
120_D216_A1.1870.99
108_I233_L1.1840.99
111_V131_E1.1800.99
181_C249_I1.1790.99
76_Q214_K1.1650.99
88_Y94_I1.1640.99
165_V255_V1.1580.99
135_P167_G1.1550.99
14_R18_E1.1460.98
121_I213_R1.1430.98
16_A23_I1.1380.98
166_I227_I1.1290.98
112_L228_I1.1280.98
118_A121_I1.1170.98
97_S241_A1.1150.98
90_D256_A1.1110.98
134_L170_A1.1070.98
182_A250_E1.1060.98
183_M234_G1.1050.98
104_I245_F1.0910.98
177_D180_N1.0880.98
54_R83_F1.0800.98
9_L26_L1.0730.98
193_S235_P1.0620.98
117_P121_I1.0480.97
248_H251_G1.0460.97
124_D128_N1.0450.97
142_S160_S1.0450.97
126_L208_A1.0400.97
171_Q175_G1.0350.97
5_T8_Q1.0270.97
150_Q153_D1.0250.97
43_H47_Q1.0230.97
151_F155_V1.0180.97
7_E11_A1.0130.97
111_V227_I1.0110.97
69_Q210_W1.0030.96
137_L247_A0.9920.96
130_I205_L0.9880.96
47_Q51_A0.9780.96
172_R175_G0.9730.96
9_L30_I0.9590.95
112_L130_I0.9580.95
86_M161_C0.9570.95
181_C251_G0.9520.95
132_W170_A0.9440.95
108_I249_I0.9400.95
132_W169_P0.9390.95
59_R226_D0.9350.95
247_A255_V0.9350.95
182_A198_S0.9320.95
33_D152_V0.9310.95
9_L29_L0.9240.94
68_V236_M0.9180.94
78_D159_A0.9170.94
68_V154_T0.9160.94
112_L224_T0.9140.94
27_R148_S0.9120.94
5_T9_L0.9120.94
134_L166_I0.9100.94
127_Y203_H0.9090.94
8_Q11_A0.9050.94
43_H81_T0.9030.94
208_A230_T0.9000.94
106_A183_M0.8960.93
132_W135_P0.8950.93
56_V226_D0.8850.93
159_A162_G0.8830.93
82_L87_C0.8810.93
240_N243_D0.8720.93
208_A228_I0.8710.92
24_A28_D0.8700.92
126_L130_I0.8680.92
154_T160_S0.8630.92
15_R21_E0.8610.92
63_L69_Q0.8580.92
114_R224_T0.8570.92
35_A151_F0.8510.92
200_C207_A0.8490.92
164_Y227_I0.8410.91
164_Y229_L0.8410.91
36_E40_A0.8370.91
101_Q147_W0.8330.91
8_Q12_D0.8310.91
79_F214_K0.8290.91
186_T189_N0.8270.90
203_H206_N0.8240.90
193_S237_V0.8220.90
38_A152_V0.8220.90
178_L197_G0.8210.90
94_I163_V0.8200.90
76_Q151_F0.8190.90
33_D37_A0.8150.90
209_V213_R0.8090.89
127_Y130_I0.8060.89
82_L86_M0.8050.89
181_C197_G0.8040.89
5_T30_I0.8000.89
78_D144_I0.7970.89
27_R150_Q0.7960.89
15_R18_E0.7910.88
77_P210_W0.7910.88
98_R163_V0.7900.88
180_N198_S0.7870.88
103_R236_M0.7870.88
22_A145_R0.7830.88
106_A197_G0.7830.88
233_L249_I0.7790.88
10_A44_I0.7770.87
36_E217_S0.7760.87
68_V140_V0.7720.87
194_S199_E0.7680.87
214_K218_L0.7660.87
81_T220_E0.7650.87
81_T158_N0.7630.87
70_Q73_G0.7600.86
127_Y205_L0.7570.86
106_A234_G0.7550.86
121_I126_L0.7550.86
125_E130_I0.7550.86
60_K138_E0.7550.86
94_I257_A0.7520.86
80_G158_N0.7510.86
179_K203_H0.7500.86
130_I209_V0.7460.85
128_N132_W0.7450.85
137_L257_A0.7410.85
187_R240_N0.7390.85
200_C230_T0.7360.84
68_V235_P0.7340.84
46_V50_V0.7260.84
105_E138_E0.7240.84
57_V227_I0.7140.83
46_V81_T0.7110.82
200_C233_L0.7090.82
133_V178_L0.7080.82
11_A44_I0.7070.82
60_K105_E0.7070.82
109_A229_L0.7050.82
108_I197_G0.7040.82
82_L161_C0.7030.82
147_W162_G0.7020.82
82_L164_Y0.7020.82
117_P223_R0.6990.81
104_I239_V0.6970.81
140_V236_M0.6950.81
173_P205_L0.6920.81
112_L116_L0.6910.81
137_L245_F0.6880.80
189_N246_E0.6870.80
61_V207_A0.6860.80
221_P224_T0.6850.80
206_N209_V0.6830.80
94_I139_V0.6800.80
57_V84_A0.6780.79
10_A45_N0.6770.79
200_C232_A0.6690.78
87_C227_I0.6670.78
154_T159_A0.6650.78
125_E209_V0.6640.78
144_I149_I0.6640.78
72_L101_Q0.6630.78
108_I252_I0.6610.78
67_K199_E0.6600.77
68_V78_D0.6590.77
13_L23_I0.6590.77
189_N244_R0.6550.77
112_L129_A0.6500.76
193_S234_G0.6490.76
104_I259_F0.6470.76
144_I147_W0.6440.76
185_M234_G0.6430.76
135_P255_V0.6380.75
134_L169_P0.6340.75
104_I257_A0.6330.75
72_L147_W0.6310.74
141_G162_G0.6300.74
55_R226_D0.6290.74
129_A212_A0.6280.74
88_Y98_R0.6280.74
30_I37_A0.6260.74
48_H52_Q0.6230.73
66_P69_Q0.6230.73
90_D93_I0.6230.73
10_A41_I0.6210.73
61_V159_A0.6190.73
165_V257_A0.6180.73
8_Q21_E0.6160.73
182_A186_T0.6140.72
135_P253_G0.6130.72
48_H51_A0.6060.71
25_P149_I0.6020.71
123_F203_H0.6010.71
26_L29_L0.6010.71
82_L227_I0.6000.71
33_D41_I0.5990.70
11_A14_R0.5980.70
111_V169_P0.5980.70
212_A222_L0.5960.70
142_S157_D0.5950.70
22_A101_Q0.5910.69
30_I33_D0.5900.69
77_P211_L0.5880.69
20_G98_R0.5850.69
68_V72_L0.5850.69
91_N256_A0.5850.69
23_I29_L0.5840.69
40_A218_L0.5840.69
210_W214_K0.5830.68
44_I48_H0.5820.68
61_V64_T0.5800.68
41_I45_N0.5770.68
109_A134_L0.5770.68
78_D149_I0.5770.68
46_V56_V0.5760.67
215_M228_I0.5760.67
112_L226_D0.5750.67
62_G140_V0.5750.67
99_V139_V0.5740.67
8_Q29_L0.5720.67
42_Q143_R0.5700.67
195_G201_L0.5700.67
74_V160_S0.5690.67
96_F99_V0.5670.66
73_G142_S0.5620.66
118_A122_T0.5620.66
68_V71_Q0.5610.65
119_T216_A0.5610.65
31_G34_N0.5600.65
96_F240_N0.5590.65
59_R220_E0.5580.65
8_Q15_R0.5560.65
23_I26_L0.5470.63
50_V70_Q0.5470.63
103_R238_A0.5450.63
126_L129_A0.5450.63
116_L121_I0.5420.63
82_L138_E0.5420.63
71_Q160_S0.5420.63
103_R232_A0.5410.63
102_P141_G0.5390.62
49_D52_Q0.5380.62
123_F205_L0.5370.62
61_V214_K0.5370.62
61_V230_T0.5320.61
110_L157_D0.5320.61
24_A148_S0.5320.61
127_Y173_P0.5310.61
121_I209_V0.5300.61
72_L144_I0.5290.61
107_E230_T0.5290.61
180_N184_K0.5280.61
68_V149_I0.5280.61
26_L33_D0.5280.61
47_Q220_E0.5280.61
184_K189_N0.5280.61
47_Q50_V0.5270.61
61_V147_W0.5250.60
89_G168_G0.5250.60
28_D34_N0.5250.60
188_N192_V0.5230.60
34_N38_A0.5220.60
213_R216_A0.5220.60
116_L126_L0.5220.60
232_A236_M0.5190.59
9_L23_I0.5190.59
200_C234_G0.5160.59
149_I236_M0.5160.59
42_Q81_T0.5150.59
222_L226_D0.5140.59
131_E134_L0.5140.59
178_L203_H0.5130.59
72_L149_I0.5130.59
6_L34_N0.5100.58
83_F86_M0.5100.58
103_R140_V0.5090.58
149_I154_T0.5090.58
133_V181_C0.5080.58
64_T201_L0.5040.57
196_R199_E0.5040.57
6_L37_A0.5040.57
106_A149_I0.5040.57
12_D30_I0.5030.57
143_R253_G0.5030.57
65_H199_E0.5030.57
61_V154_T0.5030.57
187_R192_V0.5030.57
203_H215_M0.5020.57
106_A185_M0.5000.57
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2wqtA 6 0.9703 100 0.217 Contact Map
2eb4A 5 0.9665 100 0.234 Contact Map
1sawA 2 0.7509 100 0.581 Contact Map
3s52A 2 0.7732 100 0.591 Contact Map
4maqA 2 0.8439 100 0.609 Contact Map
3v77A 5 0.7993 100 0.611 Contact Map
1gttA 1 0.8959 100 0.614 Contact Map
1wzoA 4 0.8736 100 0.62 Contact Map
1nkqA 4 0.7807 100 0.636 Contact Map
3r6oA 2 0.8773 99.9 0.652 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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