YCJV - ECOLI - GREMLIN Contacts

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YCJV - Putative uncharacterized ABC transporter ATP-binding protein YcjV

UniProt:	P77481	Sequences: MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKSTTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLKMQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDEPLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGAPKTVYNQPANMFVSGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLHKPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYHAGENITIHFDMTKCHFFDAETEIAIR	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG13919
Length:	360 (311)
Sequences:	21384 (15337)
Seq/√Len:	869.7

ECOLI - YCJV UGPC MALK FBPC CYSA POTA POTG PROV YDCT METN MODC SAPD DPPD DPPF OPPD RBSA YEHX YADG OPPF DDPD ARAG MACB YHES DDPF GSIA UUP YECC TAUB FEPC MLAF CYDC HISP LPTB NIKE MDLB MSBA SAPF SSUB MDLA LSRA YTFR FHUC ETTA FECE PHNC YOJI CYDD GLNQ YHDZ GLTL THIQ SUFC ZNUC PHNK LIVG ARTP PSTB MODF YPHE FTSE NIKD XYLG LIVF ALSA LOLD BTUD MGLA CCMA YBBA FETA YDDA YEJF PHNL YNJD YBHF YBIT YHIH
BACSU - MSMX YURJ OPUCA OPUAA OPUBA YVRA METN OPPD TAGH YKFD DPPD APPF APPD YFIL YHCH YXLF YDBJ YCBN YHAQ YFMM YKPA YVFR OPPF RBSA YDIF YTRB FHUC YFMF ECFA1 YFMR SUNT YHEH YUSV YFIC ECSA SKFE BCEA YTLC YVCR TCYN YKNV YFIB BMRA YCLP YKNU YGAD CYDD YXDL YLMA ALBC YHEI MNTB YWJA SUFC NATA YKOD ECFA2 YXEO TCYC GLNQ FTSE SSUB ARTM CYDC PSTB1 YTHP YCLH YJKB YVRO PSTB2 ZNUC EXPZ YKNY YYBJ YHCG YUFO YYDI YTRE
HALSA - Q9HHQ5 Q9HR06 Q9HNZ9 Q9HMT3 Q9HMX3 Q9HMW0 BTUDA Q9HMX2 Q9HMU8 Q9HRJ6 PHNC Q9HMG1 Q9HRH8 PSTB2 Q9HSQ7 Q9HHM2 Q9HMG6 Q9HNY3 Q9HRV7 Q9HRZ9 Y2317 Y1631 PSTB1 Q9HHR7 Q9HR96 Q9HT03 Q9HS04 Q9HQA8 Q9HR21 Q9HMC1 Q9HMW1
SULSO - Q97YY4 Q97UF2 Q97UY8 Q97ZC0 Q97VC1 Q7LX64 Q97UH3 Q97VF5 Q97VH6 Q97YP3 Q97VK8 Q97UD4 Q97VK9 Q97VF4 Q97YK2 Q97X18 Q97UJ9 Q97YP7 Q97YQ7 Q97YP8 Q97Z03 Q980W0 Q97U54 Y1893 Y2030 Q97V01 Q97UH4 PSTB Q97W42 Q7LY07 Q97V00 Q97VY4 Q7LXM4 Q97VM2 Q7LX80 Q97Z56 Q97UG5 Q97UC2 Q97Z96 Q97YP4 Q97ZU0 Q980K5 Q980E6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
217_G	221_T	4.317	1.00
34_V	202_A	4.018	1.00
60_D	67_R	4.016	1.00
8_H	22_D	3.850	1.00
31_E	204_R	3.668	1.00
5_S	26_E	3.593	1.00
300_A	316_T	3.528	1.00
7_Q	60_D	3.512	1.00
120_Q	125_R	3.392	1.00
18_H	21_K	3.208	1.00
112_D	116_N	2.921	1.00
21_K	212_I	2.806	1.00
214_Q	228_N	2.803	1.00
303_S	317_T	2.768	1.00
107_A	110_V	2.635	1.00
127_Y	130_R	2.606	1.00
33_I	206_V	2.593	1.00
28_A	31_E	2.520	1.00
218_A	221_T	2.504	1.00
94_Y	98_A	2.459	1.00
6_L	9_I	2.455	1.00
94_Y	129_K	2.381	1.00
11_K	49_M	2.367	1.00
10_Q	58_G	2.363	1.00
10_Q	18_H	2.362	1.00
5_S	62_L	2.352	1.00
7_Q	62_L	2.347	1.00
114_R	152_E	2.331	1.00
8_H	24_N	2.291	1.00
10_Q	22_D	2.270	1.00
12_I	18_H	2.255	1.00
77_N	154_G	2.197	1.00
94_Y	112_D	2.090	1.00
94_Y	116_N	2.086	1.00
20_V	49_M	2.024	1.00
4_L	155_V	2.012	1.00
123_G	172_Q	1.988	1.00
9_I	56_I	1.977	1.00
199_M	220_K	1.952	1.00
47_L	191_V	1.947	1.00
64_D	154_G	1.924	1.00
49_M	56_I	1.889	1.00
119_A	128_L	1.883	1.00
209_K	214_Q	1.875	1.00
206_V	216_V	1.865	1.00
11_K	57_S	1.849	1.00
62_L	67_R	1.841	1.00
206_V	213_V	1.813	1.00
113_E	117_W	1.750	1.00
110_V	113_E	1.722	1.00
229_M	280_V	1.716	1.00
11_K	54_E	1.709	1.00
50_I	189_I	1.705	1.00
109_E	113_E	1.683	1.00
114_R	117_W	1.672	1.00
94_Y	115_V	1.670	1.00
49_M	61_L	1.628	1.00
118_A	150_V	1.619	1.00
229_M	242_F	1.583	1.00
48_R	54_E	1.539	1.00
63_I	68_M	1.538	1.00
68_M	71_V	1.525	1.00
301_K	317_T	1.493	1.00
67_R	70_D	1.491	1.00
6_L	25_L	1.468	1.00
66_K	154_G	1.465	1.00
98_A	115_V	1.462	1.00
12_I	57_S	1.454	1.00
158_M	188_M	1.452	1.00
23_F	46_T	1.439	1.00
6_L	50_I	1.420	1.00
259_L	262_P	1.416	1.00
117_W	120_Q	1.402	1.00
233_G	242_F	1.402	1.00
229_M	233_G	1.386	1.00
47_L	157_L	1.381	1.00
56_I	61_L	1.379	1.00
106_I	110_V	1.368	1.00
110_V	114_R	1.360	1.00
122_L	146_G	1.358	1.00
274_S	277_K	1.352	1.00
33_I	216_V	1.350	1.00
27_I	33_I	1.349	1.00
106_I	111_I	1.347	1.00
246_T	278_P	1.328	1.00
116_N	120_Q	1.322	1.00
242_F	280_V	1.319	1.00
92_T	131_K	1.319	1.00
220_K	224_N	1.311	1.00
91_M	99_F	1.299	1.00
66_K	71_V	1.297	1.00
305_A	314_L	1.292	1.00
25_L	206_V	1.267	1.00
7_Q	24_N	1.261	1.00
60_D	69_N	1.252	1.00
10_Q	21_K	1.248	1.00
6_L	46_T	1.234	1.00
33_I	204_R	1.221	1.00
92_T	95_D	1.221	1.00
256_T	259_L	1.211	1.00
181_H	186_T	1.185	1.00
20_V	46_T	1.166	1.00
116_N	128_L	1.162	1.00
252_F	255_E	1.150	1.00
79_A	148_A	1.146	1.00
108_K	112_D	1.136	1.00
28_A	204_R	1.133	1.00
257_L	261_I	1.132	1.00
95_D	102_K	1.129	1.00
90_H	133_G	1.128	1.00
181_H	188_M	1.127	1.00
273_E	277_K	1.126	1.00
98_A	111_I	1.124	1.00
93_V	135_L	1.122	1.00
254_T	257_L	1.115	1.00
221_T	225_Q	1.114	1.00
244_R	278_P	1.111	1.00
12_I	19_V	1.110	1.00
95_D	98_A	1.102	1.00
100_G	151_R	1.101	1.00
257_L	260_T	1.100	1.00
48_R	53_L	1.096	1.00
111_I	115_V	1.095	1.00
259_L	264_E	1.095	1.00
12_I	58_G	1.093	1.00
20_V	23_F	1.092	1.00
56_I	69_N	1.092	1.00
9_I	49_M	1.092	1.00
117_W	152_E	1.082	1.00
204_R	216_V	1.072	1.00
102_K	108_K	1.071	1.00
41_C	208_M	1.070	1.00
61_L	68_M	1.050	1.00
244_R	280_V	1.043	1.00
178_S	201_M	1.036	1.00
249_G	252_F	1.034	1.00
259_L	263_E	1.031	1.00
46_T	208_M	1.028	1.00
258_K	261_I	1.021	1.00
240_M	282_G	1.014	1.00
62_L	65_G	1.008	1.00
88_Y	100_G	1.007	1.00
213_V	216_V	1.000	1.00
96_N	147_R	0.999	1.00
12_I	15_N	0.993	1.00
25_L	216_V	0.992	1.00
8_H	58_G	0.983	1.00
29_D	64_D	0.983	1.00
269_L	273_E	0.981	1.00
71_V	75_A	0.979	1.00
192_T	198_A	0.979	1.00
31_E	203_T	0.975	1.00
245_G	279_I	0.975	1.00
79_A	153_A	0.971	1.00
207_I	222_V	0.964	1.00
263_E	266_L	0.962	1.00
117_W	121_I	0.962	1.00
104_Q	151_R	0.962	1.00
5_S	65_G	0.960	1.00
283_I	288_I	0.960	1.00
256_T	260_T	0.946	1.00
13_Y	19_V	0.946	1.00
99_F	103_M	0.945	1.00
178_S	182_Q	0.944	1.00
224_N	307_L	0.940	1.00
294_E	297_N	0.940	1.00
241_N	245_G	0.939	1.00
87_L	132_P	0.935	1.00
261_I	264_E	0.932	1.00
32_F	201_M	0.930	1.00
273_E	276_H	0.929	1.00
252_F	256_T	0.929	1.00
27_I	187_T	0.927	1.00
34_V	198_A	0.926	1.00
51_A	157_L	0.926	1.00
250_D	253_V	0.925	1.00
116_N	125_R	0.920	1.00
97_M	150_V	0.919	1.00
94_Y	128_L	0.917	1.00
258_K	263_E	0.917	1.00
152_E	186_T	0.916	1.00
258_K	262_P	0.912	1.00
190_Y	202_A	0.912	1.00
32_F	181_H	0.911	1.00
118_A	149_I	0.910	1.00
9_I	61_L	0.910	1.00
108_K	111_I	0.907	1.00
131_K	134_A	0.906	1.00
29_D	154_G	0.906	1.00
88_Y	91_M	0.905	1.00
36_V	207_I	0.904	1.00
97_M	146_G	0.902	1.00
207_I	231_V	0.902	1.00
260_T	263_E	0.902	1.00
272_Q	276_H	0.900	1.00
72_P	75_A	0.899	1.00
91_M	95_D	0.886	1.00
118_A	121_I	0.886	1.00
170_R	194_D	0.885	1.00
253_V	257_L	0.880	1.00
274_S	278_P	0.878	1.00
162_L	177_I	0.876	1.00
307_L	312_F	0.876	1.00
262_P	265_K	0.873	1.00
178_S	200_T	0.871	1.00
171_V	175_A	0.866	1.00
32_F	188_M	0.866	1.00
98_A	102_K	0.865	1.00
220_K	307_L	0.859	1.00
30_K	187_T	0.856	1.00
79_A	151_R	0.856	1.00
162_L	190_Y	0.852	1.00
112_D	115_V	0.851	1.00
276_H	279_I	0.848	1.00
209_K	230_F	0.843	1.00
6_L	61_L	0.843	1.00
180_L	186_T	0.839	1.00
97_M	119_A	0.839	1.00
156_F	186_T	0.831	1.00
267_A	271_T	0.827	1.00
130_R	134_A	0.824	1.00
118_A	122_L	0.820	1.00
223_Y	286_E	0.812	1.00
283_I	286_E	0.810	1.00
281_M	284_R	0.799	1.00
291_D	294_E	0.793	1.00
49_M	57_S	0.792	1.00
262_P	266_L	0.792	1.00
223_Y	226_P	0.790	1.00
119_A	124_L	0.789	1.00
271_T	274_S	0.787	1.00
154_G	186_T	0.783	1.00
15_N	18_H	0.778	1.00
302_I	314_L	0.776	1.00
260_T	264_E	0.775	1.00
258_K	264_E	0.771	1.00
257_L	262_P	0.768	1.00
256_T	262_P	0.767	1.00
91_M	96_N	0.766	1.00
107_A	111_I	0.766	1.00
145_L	158_M	0.765	1.00
35_F	208_M	0.763	1.00
284_R	288_I	0.762	1.00
219_P	223_Y	0.761	1.00
153_A	156_F	0.755	1.00
7_Q	26_E	0.754	1.00
35_F	206_V	0.750	1.00
30_K	185_N	0.748	1.00
106_I	114_R	0.745	1.00
255_E	258_K	0.743	1.00
14_D	18_H	0.738	1.00
257_L	264_E	0.736	1.00
278_P	282_G	0.736	1.00
160_E	193_H	0.735	1.00
240_M	243_I	0.732	1.00
35_F	46_T	0.730	1.00
83_Q	164_N	0.727	1.00
308_T	313_M	0.725	1.00
132_P	143_V	0.725	1.00
78_I	155_V	0.724	1.00
256_T	261_I	0.720	1.00
167_A	171_V	0.717	1.00
96_N	132_P	0.715	1.00
274_S	279_I	0.710	1.00
196_T	308_T	0.708	1.00
115_V	118_A	0.702	1.00
198_A	202_A	0.699	1.00
63_I	78_I	0.698	1.00
209_K	212_I	0.698	1.00
270_K	274_S	0.696	1.00
269_L	272_Q	0.695	1.00
191_V	195_Q	0.688	1.00
290_P	293_Q	0.686	1.00
44_S	48_R	0.686	1.00
261_I	266_L	0.686	1.00
41_C	46_T	0.685	1.00
308_T	311_E	0.685	1.00
257_L	263_E	0.683	1.00
168_K	233_G	0.683	1.00
79_A	188_M	0.683	1.00
173_M	176_E	0.679	1.00
38_P	41_C	0.677	1.00
22_D	58_G	0.676	1.00
158_M	190_Y	0.676	1.00
33_I	189_I	0.675	1.00
251_K	255_E	0.673	1.00
276_H	283_I	0.672	1.00
261_I	265_K	0.669	1.00
271_T	275_L	0.668	1.00
102_K	111_I	0.666	1.00
97_M	100_G	0.665	1.00
125_R	128_L	0.663	1.00
269_L	276_H	0.660	1.00
304_V	307_L	0.659	1.00
167_A	174_R	0.658	1.00
248_D	253_V	0.658	1.00
243_I	247_I	0.656	1.00
204_R	215_Q	0.656	1.00
302_I	316_T	0.654	1.00
300_A	303_S	0.654	1.00
13_Y	54_E	0.651	1.00
251_K	254_T	0.651	1.00
85_Y	164_N	0.650	1.00
286_E	290_P	0.648	1.00
119_A	122_L	0.648	1.00
260_T	265_K	0.646	1.00
276_H	281_M	0.638	1.00
298_I	301_K	0.636	1.00
255_E	259_L	0.636	1.00
267_A	270_K	0.636	1.00
21_K	210_D	0.635	1.00
49_M	54_E	0.633	1.00
179_K	304_V	0.633	1.00
124_L	127_Y	0.632	1.00
87_L	96_N	0.631	1.00
196_T	200_T	0.631	1.00
51_A	80_M	0.631	1.00
291_D	295_E	0.624	1.00
11_K	45_T	0.623	1.00
94_Y	97_M	0.619	1.00
93_V	143_V	0.616	1.00
114_R	118_A	0.615	1.00
92_T	129_K	0.615	1.00
126_E	139_Q	0.613	1.00
268_V	271_T	0.613	1.00
100_G	147_R	0.613	1.00
83_Q	137_G	0.612	1.00
250_D	255_E	0.611	1.00
259_L	265_K	0.610	1.00
176_E	179_K	0.608	1.00
32_F	190_Y	0.608	1.00
171_V	196_T	0.602	1.00
94_Y	111_I	0.601	1.00
109_E	112_D	0.601	1.00
208_M	213_V	0.600	1.00
281_M	285_P	0.599	1.00
142_R	165_L	0.597	1.00
295_E	301_K	0.597	1.00
171_V	237_S	0.595	1.00
118_A	146_G	0.595	1.00
285_P	289_H	0.594	1.00
220_K	312_F	0.593	1.00
253_V	256_T	0.591	1.00
297_N	300_A	0.589	1.00
301_K	305_A	0.589	1.00
175_A	304_V	0.587	1.00
282_G	285_P	0.587	1.00
178_S	307_L	0.587	1.00
252_F	257_L	0.586	1.00
12_I	21_K	0.585	1.00
269_L	274_S	0.584	1.00
256_T	264_E	0.584	1.00
242_F	245_G	0.583	1.00
304_V	315_Y	0.582	1.00
285_P	288_I	0.582	1.00
256_T	263_E	0.581	1.00
257_L	265_K	0.580	1.00
231_V	235_I	0.580	1.00
23_F	213_V	0.580	1.00
164_N	174_R	0.580	1.00
157_L	189_I	0.580	1.00
25_L	213_V	0.577	1.00
175_A	315_Y	0.577	1.00
238_P	242_F	0.576	1.00
305_A	308_T	0.576	1.00
207_I	226_P	0.575	1.00
31_E	216_V	0.573	1.00
222_V	226_P	0.571	1.00
278_P	284_R	0.570	1.00
239_A	243_I	0.570	1.00
177_I	188_M	0.569	1.00
253_V	258_K	0.569	1.00
277_K	284_R	0.569	1.00
295_E	299_S	0.568	1.00
305_A	312_F	0.567	1.00
251_K	258_K	0.566	1.00
217_G	222_V	0.565	1.00
239_A	242_F	0.565	1.00
18_H	22_D	0.565	1.00
156_F	188_M	0.565	1.00
259_L	266_L	0.565	1.00
276_H	284_R	0.564	1.00
302_I	309_G	0.563	1.00
294_E	299_S	0.563	1.00
219_P	222_V	0.562	1.00
113_E	116_N	0.562	1.00
294_E	298_I	0.561	1.00
291_D	296_N	0.560	1.00
270_K	273_E	0.559	1.00
272_Q	284_R	0.559	1.00
272_Q	275_L	0.558	1.00
279_I	283_I	0.555	1.00
163_S	167_A	0.555	1.00
248_D	251_K	0.555	1.00
230_F	233_G	0.554	1.00
127_Y	139_Q	0.553	1.00
205_I	219_P	0.552	1.00
248_D	258_K	0.551	1.00
275_L	279_I	0.551	1.00
68_M	78_I	0.549	1.00
99_F	102_K	0.548	1.00
270_K	275_L	0.548	1.00
312_F	315_Y	0.547	1.00
230_F	234_F	0.546	1.00
170_R	197_E	0.546	1.00
226_P	231_V	0.544	1.00
155_V	187_T	0.544	1.00
88_Y	96_N	0.543	1.00
60_D	70_D	0.543	1.00
276_H	280_V	0.542	1.00
269_L	275_L	0.542	1.00
237_S	313_M	0.541	1.00
172_Q	176_E	0.540	1.00
281_M	288_I	0.540	1.00
267_A	274_S	0.539	1.00
77_N	104_Q	0.538	1.00
241_N	244_R	0.534	1.00
283_I	290_P	0.533	1.00
250_D	254_T	0.533	1.00
55_E	70_D	0.532	1.00
269_L	283_I	0.532	1.00
253_V	260_T	0.532	1.00
303_S	315_Y	0.530	1.00
249_G	256_T	0.529	1.00
272_Q	278_P	0.529	1.00
10_Q	20_V	0.528	1.00
111_I	114_R	0.526	1.00
123_G	176_E	0.525	1.00
241_N	247_I	0.524	1.00
89_P	133_G	0.524	1.00
270_K	277_K	0.524	1.00
254_T	259_L	0.524	1.00
274_S	283_I	0.523	1.00
249_G	254_T	0.523	1.00
191_V	194_D	0.523	1.00
71_V	74_K	0.522	1.00
81_V	141_Q	0.522	1.00
39_S	160_E	0.522	1.00
35_F	189_I	0.522	1.00
195_Q	234_F	0.518	1.00
124_L	128_L	0.517	1.00
245_G	252_F	0.517	1.00
292_A	295_E	0.516	1.00
13_Y	45_T	0.515	1.00
232_S	236_G	0.515	1.00
268_V	275_L	0.513	1.00
288_I	291_D	0.513	1.00
278_P	292_A	0.513	1.00
113_E	120_Q	0.512	1.00
81_V	148_A	0.512	1.00
32_F	177_I	0.511	1.00
81_V	145_L	0.510	1.00
11_K	20_V	0.510	1.00
84_N	140_R	0.510	1.00
250_D	257_L	0.509	1.00
181_H	203_T	0.509	1.00
179_K	303_S	0.509	1.00
39_S	193_H	0.507	1.00
284_R	287_D	0.507	1.00
305_A	309_G	0.506	1.00
254_T	258_K	0.505	1.00
29_D	185_N	0.504	1.00
123_G	126_E	0.504	1.00
69_N	73_A	0.501	1.00
22_D	212_I	0.500	1.00
4_L	63_I	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
1v43A	2	0.9639	100	0.163	Contact Map
2it1A	1	0.9944	100	0.164	Contact Map
1g291	4	0.9972	100	0.174	Contact Map
2yyzA	1	0.9806	100	0.174	Contact Map
3rlfA	2	0.9889	100	0.178	Contact Map
1oxxK	1	0.9444	100	0.188	Contact Map
3fvqA	2	0.95	100	0.21	Contact Map
4tquS	2	0.9917	100	0.212	Contact Map
1z47A	2	0.9472	100	0.214	Contact Map
3d31A	2	0.9417	100	0.223	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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