GREMLIN Database
HYFF - Hydrogenase-4 component F
UniProt: P77437 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG14214
Length: 526 (457)
Sequences: 19943 (12333)
Seq/√Len: 576.9

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
73_D271_R4.8221.00
353_L390_G4.6661.00
128_L141_A4.4361.00
194_M198_E3.6731.00
80_L131_T3.6641.00
154_G165_A3.6561.00
346_N401_N3.2541.00
309_D368_R3.2171.00
167_K244_D3.1851.00
87_G123_L3.0581.00
297_V428_L2.9321.00
343_H405_S2.9061.00
192_S198_E2.8731.00
194_M197_A2.7741.00
308_R371_N2.7471.00
17_F39_H2.6881.00
314_L358_S2.6781.00
388_G440_A2.6511.00
367_T372_V2.6441.00
83_L130_V2.6381.00
15_L124_F2.6171.00
293_G330_G2.6051.00
80_L127_L2.5071.00
125_T144_A2.5041.00
182_G224_F2.4671.00
235_L326_A2.4231.00
369_D372_V2.3891.00
391_A436_L2.3561.00
94_S115_Y2.3511.00
41_L88_F2.3151.00
327_V425_L2.2431.00
313_L370_L2.2421.00
71_H133_N2.1881.00
305_L434_A2.1871.00
84_G127_L2.1691.00
262_L318_S2.1301.00
287_R291_I1.9511.00
328_E409_T1.9461.00
380_M383_T1.9371.00
317_S435_G1.9201.00
342_L405_S1.9101.00
298_A428_L1.8841.00
308_R369_D1.8751.00
438_R442_R1.8751.00
230_G235_L1.8451.00
301_A320_E1.8421.00
73_D76_G1.8061.00
44_T85_V1.7871.00
459_W463_S1.7871.00
290_L330_G1.7641.00
164_A248_E1.7521.00
82_I348_S1.7441.00
226_L230_G1.7161.00
135_L272_Y1.7151.00
310_I439_M1.7131.00
138_M268_V1.7081.00
294_M424_L1.7001.00
234_G242_L1.6851.00
410_I425_L1.6691.00
135_L199_M1.6621.00
349_L471_M1.6601.00
185_L221_A1.6561.00
383_T456_D1.6521.00
270_I329_L1.6471.00
284_F287_R1.6461.00
22_F25_R1.6461.00
307_Q312_R1.6441.00
331_I336_G1.6401.00
28_R31_A1.6351.00
339_A412_A1.6331.00
377_L384_A1.6211.00
76_G267_L1.5871.00
198_E201_I1.5771.00
189_N221_A1.5631.00
236_F295_L1.5471.00
78_L345_L1.5421.00
118_F151_F1.5331.00
470_V474_A1.5211.00
132_S137_V1.5201.00
30_S33_R1.5151.00
43_I47_L1.5111.00
129_V264_C1.5041.00
389_G469_L1.4731.00
216_T220_L1.4691.00
71_H74_G1.4601.00
41_L45_L1.4581.00
301_A428_L1.4551.00
387_F391_A1.4521.00
235_L292_F1.4471.00
187_Y193_V1.4441.00
134_N194_M1.4421.00
329_L340_A1.4231.00
133_N197_A1.4171.00
182_G228_G1.4161.00
45_L49_L1.4151.00
30_S34_T1.4131.00
47_L84_G1.4021.00
29_L33_R1.4001.00
289_L330_G1.4001.00
11_L124_F1.4001.00
49_L53_V1.3981.00
91_G116_Y1.3961.00
269_L326_A1.3931.00
152_L253_V1.3911.00
11_L14_P1.3861.00
31_A34_T1.3821.00
458_G461_T1.3811.00
229_F268_V1.3801.00
411_T415_A1.3771.00
325_V340_A1.3761.00
387_F439_M1.3751.00
226_L289_L1.3721.00
40_S43_I1.3701.00
91_G119_F1.3681.00
466_V470_V1.3541.00
328_E336_G1.3421.00
184_V188_A1.3371.00
270_I340_A1.3341.00
286_N290_L1.3301.00
134_N193_V1.3271.00
182_G221_A1.3271.00
354_L390_G1.3261.00
481_Q485_R1.3141.00
384_A445_M1.3131.00
182_G225_L1.3081.00
350_A393_A1.3081.00
39_H43_I1.3001.00
38_L42_G1.2961.00
159_R248_E1.2951.00
160_S248_E1.2941.00
384_A440_A1.2671.00
72_I131_T1.2661.00
93_Y356_C1.2631.00
467_I471_M1.2611.00
328_E340_A1.2521.00
69_W133_N1.2501.00
331_I413_G1.2431.00
327_V413_G1.2421.00
40_S44_T1.2371.00
464_P467_I1.2341.00
244_D312_R1.2341.00
86_I348_S1.2331.00
78_L341_L1.2311.00
139_W180_L1.2241.00
370_L443_V1.2221.00
171_I240_A1.2111.00
413_G422_I1.2081.00
86_I352_T1.2071.00
459_W462_T1.2061.00
179_G228_G1.2061.00
346_N405_S1.2001.00
293_G323_G1.1951.00
33_R37_V1.1891.00
18_S22_F1.1841.00
49_L52_W1.1831.00
290_L421_I1.1781.00
299_V303_F1.1771.00
79_F344_I1.1751.00
48_I52_W1.1731.00
96_G99_R1.1701.00
138_M196_Q1.1671.00
25_R113_C1.1611.00
230_G292_F1.1591.00
460_L464_P1.1561.00
89_L467_I1.1551.00
386_L469_L1.1531.00
40_S47_L1.1521.00
18_S121_L1.1501.00
394_L436_L1.1431.00
61_G67_G1.1431.00
432_V435_G1.1391.00
382_F466_V1.1381.00
482_P485_R1.1381.00
47_L51_L1.1371.00
390_G469_L1.1351.00
414_L422_I1.1321.00
135_L183_T1.1311.00
362_L368_R1.1281.00
185_L217_L1.1211.00
391_A440_A1.1211.00
163_E167_K1.1191.00
291_I295_L1.1151.00
471_M475_M1.1081.00
183_T225_L1.0981.00
357_G387_F1.0931.00
386_L465_M1.0891.00
57_A61_G1.0841.00
320_E432_V1.0821.00
36_T116_Y1.0801.00
189_N217_L1.0791.00
122_F126_M1.0651.00
88_F92_I1.0651.00
347_H351_K1.0591.00
423_V427_L1.0591.00
388_G444_L1.0581.00
84_G88_F1.0581.00
72_I77_G1.0551.00
75_L270_I1.0501.00
408_M411_T1.0501.00
236_F299_V1.0501.00
158_Q161_S1.0501.00
339_A408_M1.0491.00
179_G183_T1.0481.00
382_F385_V1.0461.00
233_T266_L1.0451.00
27_R30_S1.0431.00
59_D63_I1.0421.00
357_G465_M1.0391.00
75_L341_L1.0381.00
293_G327_V1.0351.00
44_T84_G1.0311.00
119_F256_L1.0291.00
175_G231_T1.0281.00
336_G412_A1.0271.00
46_L50_A1.0241.00
136_I193_V1.0211.00
10_L14_P1.0191.00
420_L423_V1.0161.00
392_L397_M1.0161.00
85_V89_L1.0151.00
383_T457_L1.0151.00
364_K380_M1.0141.00
96_G460_L1.0141.00
76_G271_R1.0121.00
387_F444_L1.0101.00
35_V39_H1.0071.00
120_H124_F1.0061.00
258_S318_S0.9981.00
231_T238_M0.9951.00
251_S311_K0.9951.00
374_C449_P0.9881.00
266_L325_V0.9871.00
219_L285_P0.9841.00
57_A60_A0.9791.00
370_L442_R0.9791.00
221_A225_L0.9781.00
8_A11_L0.9771.00
34_T37_V0.9771.00
32_T36_T0.9761.00
44_T48_I0.9751.00
93_Y359_G0.9661.00
219_L284_F0.9661.00
300_A323_G0.9661.00
126_M260_V0.9651.00
301_A435_G0.9651.00
133_N194_M0.9641.00
190_A199_M0.9631.00
12_L16_L0.9621.00
386_L466_V0.9601.00
37_V40_S0.9601.00
186_V221_A0.9591.00
149_S261_L0.9591.00
469_L473_L0.9581.00
83_L123_L0.9571.00
321_N343_H0.9551.00
86_I259_A0.9551.00
360_N383_T0.9501.00
226_L269_L0.9501.00
216_T219_L0.9481.00
283_D286_N0.9461.00
58_A61_G0.9461.00
302_F431_L0.9451.00
215_P219_L0.9441.00
197_A201_I0.9421.00
361_V376_M0.9391.00
178_F227_I0.9381.00
161_S248_E0.9371.00
401_N475_M0.9371.00
318_S347_H0.9361.00
10_L46_L0.9351.00
36_T40_S0.9341.00
310_I358_S0.9341.00
469_L472_M0.9331.00
360_N457_L0.9331.00
125_T148_S0.9331.00
70_L131_T0.9321.00
428_L431_L0.9321.00
255_A356_C0.9291.00
192_S199_M0.9281.00
44_T47_L0.9281.00
382_F386_L0.9251.00
59_D62_E0.9231.00
56_T59_D0.9221.00
36_T39_H0.9211.00
301_A431_L0.9211.00
47_L50_A0.9191.00
364_K461_T0.9181.00
223_V227_I0.9161.00
43_I46_L0.9141.00
365_Y373_V0.9131.00
153_V245_A0.9111.00
40_S46_L0.9101.00
297_V323_G0.9101.00
79_F267_L0.9081.00
214_D218_M0.9071.00
162_L165_A0.9061.00
374_C442_R0.9061.00
321_N435_G0.9061.00
295_L299_V0.9051.00
384_A444_L0.9051.00
353_L468_L0.9051.00
196_Q200_A0.9051.00
338_F487_L0.9021.00
333_G336_G0.9021.00
254_S262_L0.9011.00
121_L148_S0.8991.00
342_L346_N0.8971.00
267_L271_R0.8961.00
18_S117_G0.8951.00
363_L455_G0.8941.00
139_W143_E0.8931.00
42_G46_L0.8911.00
96_G458_G0.8901.00
407_F411_T0.8891.00
269_L329_L0.8881.00
145_T261_L0.8851.00
178_F224_F0.8851.00
187_Y190_A0.8811.00
34_T38_L0.8801.00
33_R36_T0.8801.00
243_P316_Y0.8801.00
290_L293_G0.8781.00
321_N432_V0.8751.00
87_G119_F0.8751.00
215_P218_M0.8701.00
360_N465_M0.8691.00
185_L189_N0.8631.00
266_L344_I0.8611.00
220_L224_F0.8611.00
122_F149_S0.8611.00
328_E339_A0.8611.00
374_C446_A0.8581.00
130_V267_L0.8541.00
310_I373_V0.8501.00
419_L423_V0.8501.00
69_W194_M0.8491.00
14_P18_S0.8481.00
440_A445_M0.8481.00
325_V344_I0.8451.00
89_L464_P0.8441.00
287_R290_L0.8431.00
401_N472_M0.8421.00
76_G80_L0.8411.00
463_S467_I0.8391.00
380_M456_D0.8371.00
313_L439_M0.8361.00
32_T35_V0.8361.00
57_A69_W0.8351.00
385_V389_G0.8351.00
367_T373_V0.8341.00
82_I345_L0.8341.00
309_D369_D0.8331.00
385_V444_L0.8311.00
350_A402_I0.8301.00
266_L326_A0.8301.00
139_W183_T0.8291.00
384_A388_G0.8251.00
126_M145_T0.8231.00
214_D217_L0.8231.00
304_I435_G0.8231.00
192_S196_Q0.8221.00
436_L439_M0.8191.00
26_K29_L0.8191.00
126_M261_L0.8181.00
115_Y252_P0.8181.00
297_V327_V0.8171.00
81_A85_V0.8171.00
95_I460_L0.8161.00
258_S351_K0.8141.00
294_M327_V0.8121.00
31_A35_V0.8121.00
379_I448_P0.8091.00
90_T356_C0.8081.00
290_L294_M0.8061.00
314_L354_L0.8061.00
324_L409_T0.8031.00
40_S91_G0.8021.00
26_K30_S0.8011.00
301_A317_S0.8011.00
343_H350_A0.8001.00
59_D67_G0.7991.00
125_T141_A0.7991.00
55_Q58_A0.7981.00
18_S21_C0.7971.00
13_T17_F0.7961.00
19_L22_F0.7951.00
333_G412_A0.7951.00
127_L131_T0.7901.00
8_A58_A0.7901.00
462_T466_V0.7901.00
457_L462_T0.7871.00
210_S213_L0.7861.00
11_L70_L0.7861.00
142_I179_G0.7851.00
18_S124_F0.7851.00
150_A165_A0.7831.00
300_A303_F0.7821.00
247_S258_S0.7811.00
173_T176_V0.7781.00
153_V257_L0.7781.00
334_P338_F0.7771.00
222_F285_P0.7771.00
160_S163_E0.7761.00
9_L13_T0.7761.00
393_A400_F0.7751.00
290_L327_V0.7731.00
83_L126_M0.7721.00
66_A136_I0.7721.00
160_S247_S0.7701.00
219_L223_V0.7691.00
317_S347_H0.7681.00
449_P452_V0.7651.00
90_T255_A0.7611.00
36_T43_I0.7611.00
77_G81_A0.7601.00
378_K381_P0.7551.00
266_L322_M0.7541.00
50_A53_V0.7541.00
75_L267_L0.7501.00
47_L80_L0.7491.00
145_T264_C0.7491.00
270_I337_I0.7481.00
86_I256_L0.7481.00
298_A302_F0.7481.00
391_A439_M0.7441.00
29_L32_T0.7371.00
399_P477_T0.7351.00
150_A172_C0.7351.00
69_W134_N0.7341.00
300_A320_E0.7341.00
288_L291_I0.7341.00
331_I416_R0.7331.00
324_L428_L0.7331.00
97_Y252_P0.7321.00
153_V249_A0.7321.00
389_G473_L0.7321.00
193_V198_E0.7311.00
456_D462_T0.7301.00
57_A62_E0.7291.00
17_F120_H0.7281.00
113_C117_G0.7271.00
122_F261_L0.7261.00
134_N137_V0.7261.00
28_R32_T0.7241.00
22_F117_G0.7231.00
17_F21_C0.7221.00
293_G326_A0.7221.00
350_A472_M0.7221.00
122_F256_L0.7111.00
396_G406_E0.7111.00
43_I50_A0.7111.00
213_L216_T0.7091.00
14_P17_F0.7091.00
254_S311_K0.7071.00
395_A433_L0.7071.00
437_V441_A0.7061.00
424_L428_L0.7031.00
41_L52_W0.7011.00
282_S286_N0.6991.00
72_I76_G0.6971.00
468_L472_M0.6951.00
272_Y276_I0.6951.00
321_N351_K0.6941.00
186_V225_L0.6941.00
212_L215_P0.6941.00
294_M298_A0.6931.00
220_L223_V0.6911.00
430_T434_A0.6911.00
307_Q316_Y0.6901.00
474_A482_P0.6901.00
303_F316_Y0.6881.00
138_M179_G0.6881.00
142_I264_C0.6881.00
336_G340_A0.6861.00
247_S309_D0.6861.00
43_I84_G0.6831.00
180_L184_V0.6811.00
48_I81_A0.6801.00
356_C464_P0.6791.00
54_V57_A0.6771.00
37_V41_L0.6731.00
463_S466_V0.6711.00
410_I422_I0.6711.00
218_M221_A0.6711.00
310_I443_V0.6701.00
175_G228_G0.6691.00
385_V388_G0.6691.00
75_L79_F0.6691.00
473_L476_G0.6691.00
133_N137_V0.6681.00
186_V199_M0.6651.00
217_L221_A0.6621.00
382_F462_T0.6621.00
27_R32_T0.6611.00
11_L131_T0.6611.00
89_L352_T0.6591.00
166_W170_I0.6591.00
393_A402_I0.6581.00
251_S359_G0.6571.00
259_A263_N0.6571.00
37_V92_I0.6561.00
135_L186_V0.6551.00
37_V44_T0.6541.00
38_L41_L0.6511.00
41_L44_T0.6511.00
16_L20_L0.6511.00
317_S351_K0.6491.00
14_P124_F0.6431.00
454_R457_L0.6421.00
242_L262_L0.6411.00
396_G402_I0.6411.00
475_M483_V0.6381.00
297_V324_L0.6381.00
249_A254_S0.6371.00
94_S97_Y0.6351.00
349_L401_N0.6341.00
317_S321_N0.6331.00
357_G386_L0.6311.00
37_V43_I0.6301.00
81_A84_G0.6301.00
353_L465_M0.6291.00
258_S347_H0.6291.00
240_A243_P0.6271.00
258_S262_L0.6241.00
410_I429_L0.6241.00
341_L487_L0.6221.00
440_A444_L0.6211.00
185_L220_L0.6211.00
400_F404_L0.6211.00
254_S347_H0.6181.00
83_L127_L0.6181.00
8_A68_L0.6181.00
143_E232_K0.6171.00
33_R43_I0.6171.00
9_L46_L0.6161.00
298_A424_L0.6151.00
156_Y159_R0.6151.00
29_L36_T0.6141.00
386_L390_G0.6121.00
226_L285_P0.6121.00
46_L49_L0.6121.00
482_P486_I0.6101.00
93_Y255_A0.6081.00
58_A62_E0.6081.00
377_L444_L0.6081.00
51_L81_A0.6071.00
71_H197_A0.6061.00
90_T252_P0.6051.00
87_G256_L0.6041.00
28_R33_R0.6031.00
293_G297_V0.6031.00
85_V471_M0.6021.00
36_T91_G0.6021.00
242_L245_A0.6011.00
434_A438_R0.6001.00
343_H346_N0.6001.00
47_L81_A0.5991.00
25_R29_L0.5971.00
94_S252_P0.5971.00
363_L461_T0.5921.00
335_L415_A0.5911.00
433_L437_V0.5911.00
301_A432_V0.5911.00
254_S257_L0.5901.00
284_F288_L0.5891.00
251_S254_S0.5881.00
115_Y253_V0.5881.00
236_F300_A0.5851.00
126_M264_C0.5841.00
303_F306_V0.5841.00
269_L276_I0.5821.00
412_A416_R0.5811.00
43_I53_V0.5811.00
345_L475_M0.5811.00
376_M380_M0.5811.00
133_N271_R0.5801.00
183_T193_V0.5801.00
375_G381_P0.5801.00
33_R95_I0.5801.00
10_L22_F0.5801.00
404_L476_G0.5781.00
79_F83_L0.5741.00
90_T119_F0.5721.00
406_E429_L0.5721.00
92_I464_P0.5721.00
123_L127_L0.5721.00
197_A200_A0.5711.00
84_G123_L0.5711.00
350_A394_L0.5711.00
103_A114_D0.5701.00
89_L468_L0.5701.00
375_G448_P0.5701.00
94_S116_Y0.5691.00
139_W176_V0.5650.99
200_A274_I0.5640.99
291_I294_M0.5640.99
259_A352_T0.5630.99
342_L408_M0.5620.99
308_R317_S0.5610.99
243_P319_V0.5600.99
360_N461_T0.5590.99
307_Q313_L0.5580.99
39_H120_H0.5570.99
247_S312_R0.5570.99
36_T120_H0.5560.99
118_F148_S0.5560.99
15_L19_L0.5560.99
82_I344_I0.5560.99
347_H355_F0.5540.99
357_G390_G0.5540.99
115_Y155_I0.5530.99
381_P384_A0.5530.99
8_A12_L0.5510.99
217_L220_L0.5510.99
38_L45_L0.5510.99
377_L388_G0.5510.99
360_N364_K0.5500.99
370_L373_V0.5500.99
37_V51_L0.5500.99
408_M484_I0.5500.99
386_L462_T0.5490.99
132_S141_A0.5460.99
226_L292_F0.5440.99
233_T269_L0.5430.99
338_F484_I0.5430.99
303_F307_Q0.5420.99
135_L196_Q0.5400.99
34_T40_S0.5400.99
481_Q484_I0.5400.99
388_G445_M0.5380.99
191_A194_M0.5380.99
321_N324_L0.5380.99
10_L13_T0.5370.99
14_P22_F0.5360.99
375_G446_A0.5360.99
163_E244_D0.5350.99
190_A194_M0.5350.99
18_S26_K0.5350.99
302_F306_V0.5340.99
224_F228_G0.5340.99
234_G262_L0.5340.99
21_C25_R0.5330.99
262_L322_M0.5320.99
250_P311_K0.5320.99
98_M101_E0.5300.99
43_I127_L0.5300.99
357_G383_T0.5300.99
263_N322_M0.5300.99
38_L43_I0.5290.99
44_T88_F0.5280.99
274_I277_C0.5280.99
41_L48_I0.5260.99
345_L483_V0.5250.99
196_Q199_M0.5250.99
404_L477_T0.5240.99
233_T265_A0.5230.99
395_A400_F0.5230.99
370_L439_M0.5230.99
388_G391_A0.5230.99
61_G65_A0.5220.99
10_L43_I0.5210.99
46_L52_W0.5200.99
310_I361_V0.5190.99
44_T50_A0.5190.99
384_A443_V0.5170.99
25_R28_R0.5170.99
390_G472_M0.5160.99
78_L487_L0.5160.99
222_F226_L0.5150.99
60_A63_I0.5150.99
92_I463_S0.5150.99
42_G47_L0.5140.99
426_L429_L0.5140.99
294_M421_I0.5130.99
235_L238_M0.5120.99
86_I260_V0.5090.99
378_K447_K0.5080.99
115_Y152_L0.5060.99
149_S152_L0.5060.99
51_L486_I0.5050.99
324_L425_L0.5040.99
398_P403_F0.5040.99
333_G337_I0.5030.99
396_G400_F0.5020.99
296_S326_A0.5000.99
318_S322_M0.5000.99
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3rkoM 1 0.903 100 0.216 Contact Map
4he8L 1 0.9525 100 0.238 Contact Map
3rkoL 1 0.9373 100 0.241 Contact Map
3rkoN 1 0.8707 100 0.251 Contact Map
4he8M 1 0.8669 100 0.259 Contact Map
4he8N 1 0.8099 100 0.312 Contact Map
4he8K 1 0.1749 39.8 0.965 Contact Map
3rkoK 1 0.1825 33.7 0.966 Contact Map
3wu2X 1 0.0703 5.6 0.976 Contact Map
4he8J 1 0.2852 4 0.978 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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