GREMLIN Database
HYFH - Hydrogenase-4 component H
UniProt: P77423 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG14216
Length: 181 (149)
Sequences: 540 (370)
Seq/√Len: 30.3

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
82_E90_I4.7911.00
157_C160_C3.2811.00
39_Q89_A3.2741.00
117_C120_C3.1211.00
131_A138_A2.9521.00
47_A55_L2.9461.00
35_L90_I2.6861.00
150_L153_Q2.0670.98
113_H127_Q2.0630.98
27_P94_N1.9680.97
131_A135_E1.9510.97
97_E106_L1.9340.96
145_Q148_E1.8930.96
147_R150_L1.8780.96
41_I87_T1.8760.96
54_A76_I1.8350.95
34_D93_T1.7900.95
134_A138_A1.7510.94
82_E92_L1.7410.94
120_C124_F1.6680.92
117_C137_L1.6120.91
120_C137_L1.5700.90
132_L140_Q1.5670.90
148_E151_W1.5370.89
126_P130_I1.4960.87
49_A84_V1.4940.87
147_R153_Q1.4570.86
138_A151_W1.4300.85
49_A80_R1.4130.84
148_E155_S1.3440.81
84_V89_A1.3310.80
25_V96_F1.3310.80
130_I134_A1.3150.79
135_E139_Q1.2660.77
120_C157_C1.2630.76
52_A74_R1.2490.76
120_C160_C1.2270.74
134_A155_S1.2240.74
151_W155_S1.2150.73
102_N105_D1.2090.73
34_D91_Q1.2020.73
133_A137_L1.2010.73
159_E162_Q1.1910.72
146_N150_L1.1620.70
117_C160_C1.1510.69
127_Q134_A1.1480.69
57_I68_W1.1200.67
146_N149_M1.1190.67
118_S152_A1.1180.67
43_C85_C1.1160.67
46_C85_C1.1160.67
78_C81_C1.1160.67
46_C50_C1.1160.67
46_C78_C1.1160.67
46_C81_C1.1160.67
81_C85_C1.1160.67
40_C43_C1.1160.67
50_C78_C1.1160.67
50_C81_C1.1160.67
50_C85_C1.1160.67
78_C85_C1.1160.67
43_C81_C1.1160.67
43_C78_C1.1160.67
40_C81_C1.1160.67
40_C78_C1.1160.67
40_C85_C1.1160.67
40_C50_C1.1160.67
40_C46_C1.1160.67
43_C46_C1.1160.67
43_C50_C1.1160.67
149_M152_A1.1120.67
27_P96_F1.1100.66
50_C75_C1.0980.66
43_C75_C1.0980.66
75_C81_C1.0980.66
40_C75_C1.0980.66
75_C85_C1.0980.66
46_C75_C1.0980.66
75_C78_C1.0980.66
49_A128_K1.0930.65
117_C157_C1.0770.64
67_T111_T1.0690.64
157_C161_K1.0660.63
36_M39_Q1.0580.63
117_C124_F1.0540.62
17_K20_F1.0420.61
13_T20_F1.0380.61
73_G77_Y1.0270.60
42_A77_Y1.0170.60
52_A76_I1.0110.59
58_Q67_T1.0040.59
117_C121_E0.9870.57
15_T30_R0.9830.57
150_L156_V0.9820.57
35_L93_T0.9820.57
147_R154_A0.9750.56
134_A139_Q0.9750.56
33_P80_R0.9740.56
69_Q88_R0.9650.56
148_E153_Q0.9560.55
146_N153_Q0.9540.55
152_A155_S0.9500.54
147_R151_W0.9330.53
31_G36_M0.9260.52
63_Q109_R0.9260.52
21_A24_E0.9250.52
15_T19_P0.9090.51
58_Q63_Q0.9050.51
16_V23_L0.8760.48
56_T105_D0.8750.48
147_R152_A0.8720.48
35_L44_G0.8700.48
108_T118_S0.8610.47
51_P76_I0.8590.47
150_L154_A0.8490.46
127_Q138_A0.8440.46
51_P86_P0.8410.46
129_T138_A0.8310.45
89_A92_L0.8280.45
122_R137_L0.8250.44
77_Y98_L0.8230.44
122_R159_E0.8230.44
59_T63_Q0.8170.44
96_F106_L0.8080.43
123_P127_Q0.8060.43
152_A158_P0.8040.43
116_R123_P0.8020.43
80_R84_V0.7870.41
15_T18_Y0.7870.41
156_V161_K0.7850.41
114_L117_C0.7830.41
33_P83_E0.7750.40
82_E97_E0.7740.40
52_A70_L0.7730.40
52_A89_A0.7700.40
39_Q90_I0.7620.40
129_T132_L0.7450.38
45_A120_C0.7410.38
144_P153_Q0.7400.38
77_Y94_N0.7380.38
135_E138_A0.7360.38
53_N105_D0.7340.37
144_P151_W0.7270.37
155_S158_P0.7250.37
12_G95_N0.7240.37
14_A122_R0.7180.36
117_C126_P0.7130.36
59_T62_Q0.7110.36
89_A98_L0.7090.36
69_Q109_R0.7070.35
99_T108_T0.7030.35
148_E152_A0.6970.35
154_A157_C0.6910.34
54_A68_W0.6910.34
74_R84_V0.6900.34
158_P161_K0.6850.34
134_A149_M0.6850.34
61_D113_H0.6830.34
120_C123_P0.6800.33
146_N155_S0.6800.33
153_Q156_V0.6780.33
51_P103_K0.6760.33
131_A134_A0.6730.33
95_N105_D0.6620.32
32_K93_T0.6610.32
31_G54_A0.6570.32
125_A129_T0.6540.32
16_V21_A0.6530.32
156_V160_C0.6520.31
36_M54_A0.6500.31
76_I80_R0.6430.31
80_R90_I0.6380.31
13_T16_V0.6350.30
134_A140_Q0.6340.30
99_T106_L0.6240.30
120_C132_L0.6140.29
138_A141_Q0.6130.29
79_G92_L0.6040.28
45_A49_A0.6040.28
71_Y76_I0.6010.28
44_G48_C0.5930.28
150_L155_S0.5930.28
22_P96_F0.5920.28
141_Q154_A0.5780.27
138_A150_L0.5780.27
28_G45_A0.5760.27
32_K95_N0.5750.26
37_P89_A0.5720.26
137_L141_Q0.5680.26
33_P38_S0.5670.26
128_K132_L0.5660.26
100_V106_L0.5650.26
120_C126_P0.5630.26
103_K107_Y0.5630.26
18_Y97_E0.5620.26
145_Q155_S0.5600.26
73_G96_F0.5540.25
45_A80_R0.5520.25
92_L120_C0.5460.25
70_L130_I0.5450.25
20_F47_A0.5360.24
29_F83_E0.5340.24
13_T59_T0.5250.23
52_A87_T0.5210.23
119_R126_P0.5210.23
144_P149_M0.5190.23
76_I84_V0.5180.23
18_Y86_P0.5150.23
130_I138_A0.5140.23
99_T128_K0.5130.23
53_N69_Q0.5130.23
131_A136_L0.5130.23
123_P132_L0.5130.23
139_Q151_W0.5110.23
72_L77_Y0.5090.23
135_E140_Q0.5080.22
33_P68_W0.5070.22
32_K100_V0.5060.22
71_Y107_Y0.5060.22
135_E149_M0.5050.22
70_L113_H0.5040.22
35_L115_Q0.5030.22
76_I92_L0.5010.22
96_F127_Q0.5000.22
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3i9v9 1 0.768 99.9 0.563 Contact Map
5c4iB 1 0.4696 99.6 0.689 Contact Map
1gteA 4 0.5525 99.6 0.69 Contact Map
4omfG 2 0.5249 99.5 0.706 Contact Map
1hfeL 2 0.8729 99.5 0.706 Contact Map
3i9v3 1 0.895 99.5 0.709 Contact Map
1xerA 2 0.5193 99.4 0.714 Contact Map
2gmhA 1 0.5856 99.4 0.715 Contact Map
1rgvA 1 0.4088 99.4 0.72 Contact Map
2c42A 2 0.8895 99.4 0.721 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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