EUTJ - ECOLI - GREMLIN Contacts

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EUTJ - Ethanolamine utilization protein EutJ

UniProt:	P77277	Sequences: MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSMVVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPPGTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVTYSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIEGQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLYAK	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_01
EcoGene:	EG14184
Length:	278 (239)
Sequences:	11458 (6483)
Seq/√Len:	419.4

ECOLI - EUTJ HSCA DNAK HSCC MREB YEGD FTSA
BACSU - DNAK MREB MBL MREBH FTSA
HALSA - DNAK

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
188_E	192_R	3.348	1.00
95_H	119_E	3.106	1.00
214_A	244_Q	3.073	1.00
142_V	209_M	3.005	1.00
164_D	208_K	2.922	1.00
175_T	204_P	2.717	1.00
255_H	258_F	2.457	1.00
41_V	81_H	2.433	1.00
189_Q	193_G	2.276	1.00
82_L	86_E	2.209	1.00
39_C	146_G	2.164	1.00
203_K	207_E	2.128	1.00
99_S	264_I	2.093	1.00
80_R	84_T	2.055	1.00
98_T	113_L	2.045	1.00
75_V	115_S	2.032	1.00
179_N	201_A	2.015	1.00
239_E	243_K	2.009	1.00
242_R	249_Q	1.990	1.00
38_T	147_G	1.964	1.00
210_A	244_Q	1.911	1.00
233_M	257_L	1.906	1.00
153_I	216_H	1.900	1.00
138_N	155_K	1.858	1.00
231_S	234_Q	1.848	1.00
172_I	202_V	1.843	1.00
172_I	205_V	1.821	1.00
197_E	200_P	1.816	1.00
240_L	243_K	1.800	1.00
210_A	240_L	1.793	1.00
194_H	197_E	1.778	1.00
238_A	252_L	1.766	1.00
44_M	265_A	1.765	1.00
137_D	223_T	1.730	1.00
177_A	184_L	1.719	1.00
203_K	240_L	1.709	1.00
144_I	149_T	1.701	1.00
198_I	234_Q	1.676	1.00
107_R	111_N	1.667	1.00
195_G	199_W	1.657	1.00
206_Y	236_G	1.643	1.00
175_T	205_V	1.637	1.00
202_V	234_Q	1.612	1.00
95_H	121_S	1.611	1.00
111_N	114_E	1.603	1.00
153_I	220_Q	1.593	1.00
44_M	261_P	1.592	1.00
140_G	153_I	1.563	1.00
258_F	262_L	1.559	1.00
79_R	83_D	1.539	1.00
68_V	73_G	1.537	1.00
43_S	81_H	1.530	1.00
76_T	79_R	1.527	1.00
202_V	206_Y	1.494	1.00
215_R	219_G	1.477	1.00
210_A	214_A	1.472	1.00
137_D	224_D	1.472	1.00
125_D	128_T	1.469	1.00
175_T	178_G	1.453	1.00
211_D	215_R	1.448	1.00
159_V	162_S	1.426	1.00
32_L	43_S	1.410	1.00
206_Y	240_L	1.395	1.00
202_V	205_V	1.388	1.00
186_E	189_Q	1.385	1.00
200_P	203_K	1.384	1.00
74_A	109_S	1.372	1.00
255_H	262_L	1.360	1.00
83_D	87_Q	1.354	1.00
51_Q	58_D	1.353	1.00
144_I	172_I	1.351	1.00
176_L	187_A	1.350	1.00
225_L	245_F	1.341	1.00
82_L	118_L	1.338	1.00
151_I	216_H	1.327	1.00
238_A	242_R	1.325	1.00
110_I	114_E	1.324	1.00
54_A	57_L	1.313	1.00
62_V	67_I	1.309	1.00
224_D	251_H	1.301	1.00
124_L	267_S	1.299	1.00
169_G	231_S	1.295	1.00
177_A	187_A	1.295	1.00
86_E	92_R	1.280	1.00
240_L	244_Q	1.280	1.00
71_F	111_N	1.279	1.00
36_L	74_A	1.274	1.00
47_D	50_G	1.270	1.00
180_R	187_A	1.264	1.00
42_V	261_P	1.262	1.00
213_V	241_F	1.249	1.00
174_L	178_G	1.244	1.00
182_I	186_E	1.241	1.00
179_N	197_E	1.209	1.00
236_G	239_E	1.193	1.00
140_G	151_I	1.192	1.00
101_P	104_T	1.190	1.00
199_W	239_E	1.189	1.00
203_K	236_G	1.181	1.00
227_L	241_F	1.176	1.00
149_T	209_M	1.168	1.00
33_G	264_I	1.163	1.00
100_F	123_V	1.162	1.00
31_W	268_G	1.148	1.00
172_I	231_S	1.143	1.00
36_L	78_V	1.141	1.00
144_I	237_V	1.136	1.00
40_D	257_L	1.134	1.00
142_V	149_T	1.123	1.00
172_I	198_I	1.113	1.00
68_V	76_T	1.112	1.00
80_R	83_D	1.096	1.00
124_L	129_A	1.095	1.00
62_V	65_D	1.081	1.00
151_I	212_I	1.080	1.00
72_F	115_S	1.070	1.00
36_L	109_S	1.070	1.00
215_R	218_E	1.068	1.00
174_L	177_A	1.055	1.00
126_E	150_G	1.055	1.00
46_V	269_R	1.051	1.00
214_A	218_E	1.040	1.00
174_L	184_L	1.030	1.00
216_H	220_Q	1.028	1.00
207_E	240_L	1.026	1.00
102_P	127_P	1.024	1.00
130_V	228_A	1.022	1.00
185_E	189_Q	1.014	1.00
106_P	125_D	1.014	1.00
33_G	261_P	1.013	1.00
267_S	270_E	1.013	1.00
207_E	211_D	1.012	1.00
140_G	213_V	1.011	1.00
95_H	120_V	1.008	1.00
172_I	234_Q	1.006	1.00
227_L	232_C	1.000	1.00
52_P	55_V	0.996	1.00
163_A	215_R	0.993	1.00
31_W	121_S	0.992	1.00
239_E	242_R	0.981	1.00
175_T	202_V	0.975	1.00
97_A	268_G	0.973	1.00
241_F	250_V	0.973	1.00
198_I	231_S	0.972	1.00
122_H	267_S	0.968	1.00
198_I	202_V	0.962	1.00
236_G	240_L	0.961	1.00
130_V	260_T	0.958	1.00
161_Y	216_H	0.955	1.00
53_V	57_L	0.949	1.00
110_I	123_V	0.943	1.00
114_E	119_E	0.942	1.00
143_D	150_G	0.940	1.00
147_G	150_G	0.940	1.00
186_E	190_Y	0.931	1.00
207_E	210_A	0.931	1.00
134_L	226_W	0.928	1.00
197_E	201_A	0.925	1.00
228_A	259_M	0.919	1.00
82_L	96_A	0.912	1.00
45_V	89_F	0.912	1.00
75_V	79_R	0.906	1.00
81_H	84_T	0.905	1.00
144_I	231_S	0.891	1.00
173_S	188_E	0.888	1.00
207_E	244_Q	0.884	1.00
83_D	86_E	0.876	1.00
71_F	108_I	0.870	1.00
33_G	265_A	0.864	1.00
199_W	203_K	0.860	1.00
187_A	190_Y	0.856	1.00
202_V	236_G	0.855	1.00
79_R	82_L	0.854	1.00
52_P	57_L	0.851	1.00
195_G	235_P	0.838	1.00
111_N	115_S	0.837	1.00
41_V	77_I	0.833	1.00
51_Q	54_A	0.831	1.00
211_D	214_A	0.821	1.00
196_E	199_W	0.820	1.00
70_D	74_A	0.820	1.00
184_L	187_A	0.816	1.00
173_S	184_L	0.814	1.00
131_A	154_V	0.810	1.00
187_A	192_R	0.808	1.00
33_G	37_G	0.806	1.00
195_G	198_I	0.802	1.00
128_T	154_V	0.799	1.00
191_K	231_S	0.794	1.00
84_T	87_Q	0.791	1.00
196_E	200_P	0.788	1.00
144_I	209_M	0.788	1.00
138_N	223_T	0.785	1.00
71_F	75_V	0.783	1.00
60_A	65_D	0.782	1.00
34_V	96_A	0.781	1.00
201_A	204_P	0.779	1.00
46_V	50_G	0.778	1.00
55_V	58_D	0.773	1.00
208_K	211_D	0.770	1.00
72_F	75_V	0.768	1.00
100_F	106_P	0.766	1.00
107_R	110_I	0.766	1.00
164_D	167_T	0.763	1.00
63_V	66_G	0.762	1.00
106_P	110_I	0.758	1.00
172_I	206_Y	0.758	1.00
109_S	112_V	0.746	1.00
134_L	259_M	0.744	1.00
132_D	267_S	0.741	1.00
166_A	171_H	0.738	1.00
242_R	250_V	0.736	1.00
37_G	49_D	0.727	1.00
217_I	220_Q	0.723	1.00
216_H	219_G	0.719	1.00
86_E	93_F	0.717	1.00
60_A	67_I	0.716	1.00
72_F	76_T	0.714	1.00
133_L	262_L	0.714	1.00
60_A	63_V	0.713	1.00
177_A	181_R	0.712	1.00
135_Q	157_G	0.711	1.00
199_W	236_G	0.710	1.00
167_T	171_H	0.708	1.00
245_F	248_L	0.703	1.00
74_A	78_V	0.699	1.00
73_G	76_T	0.698	1.00
138_N	222_I	0.698	1.00
167_T	208_K	0.697	1.00
98_T	109_S	0.690	1.00
38_T	148_T	0.685	1.00
153_I	222_I	0.685	1.00
189_Q	192_R	0.685	1.00
76_T	80_R	0.684	1.00
143_D	147_G	0.680	1.00
126_E	146_G	0.679	1.00
94_S	119_E	0.678	1.00
100_F	125_D	0.678	1.00
106_P	123_V	0.672	1.00
262_L	266_S	0.672	1.00
77_I	81_H	0.671	1.00
133_L	259_M	0.671	1.00
127_P	148_T	0.668	0.99
212_I	216_H	0.664	0.99
98_T	101_P	0.664	0.99
42_V	258_F	0.660	0.99
176_L	179_N	0.659	0.99
108_I	112_V	0.658	0.99
183_S	190_Y	0.656	0.99
210_A	213_V	0.655	0.99
127_P	130_V	0.654	0.99
99_S	102_P	0.653	0.99
51_Q	55_V	0.652	0.99
155_K	160_T	0.646	0.99
34_V	78_V	0.644	0.99
176_L	201_A	0.642	0.99
205_V	208_K	0.640	0.99
142_V	213_V	0.639	0.99
136_L	157_G	0.636	0.99
108_I	111_N	0.634	0.99
186_E	193_G	0.633	0.99
86_E	90_G	0.633	0.99
183_S	187_A	0.632	0.99
32_L	85_L	0.628	0.99
202_V	231_S	0.626	0.99
46_V	265_A	0.625	0.99
65_D	68_V	0.625	0.99
99_S	260_T	0.624	0.99
191_K	234_Q	0.619	0.99
74_A	77_I	0.618	0.99
214_A	245_F	0.618	0.99
32_L	93_F	0.614	0.99
142_V	151_I	0.613	0.99
85_L	89_F	0.610	0.99
54_A	58_D	0.608	0.99
126_E	148_T	0.606	0.99
71_F	115_S	0.605	0.99
225_L	248_L	0.602	0.99
32_L	96_A	0.602	0.99
183_S	191_K	0.602	0.99
180_R	183_S	0.599	0.99
112_V	116_A	0.594	0.99
126_E	260_T	0.593	0.99
175_T	201_A	0.592	0.99
144_I	232_C	0.592	0.99
187_A	191_K	0.592	0.99
59_W	62_V	0.586	0.99
163_A	219_G	0.585	0.99
213_V	244_Q	0.583	0.99
31_W	95_H	0.583	0.99
227_L	237_V	0.582	0.99
75_V	112_V	0.582	0.99
124_L	128_T	0.581	0.99
204_P	207_E	0.580	0.99
53_V	56_C	0.580	0.99
52_P	56_C	0.577	0.99
102_P	109_S	0.576	0.99
251_H	259_M	0.576	0.99
104_T	109_S	0.575	0.99
247_A	250_V	0.574	0.98
100_F	109_S	0.574	0.98
143_D	146_G	0.574	0.98
170_H	173_S	0.571	0.98
173_S	187_A	0.569	0.98
178_G	181_R	0.569	0.98
32_L	89_F	0.568	0.98
164_D	168_G	0.567	0.98
70_D	73_G	0.567	0.98
232_C	252_L	0.566	0.98
233_M	255_H	0.566	0.98
213_V	245_F	0.565	0.98
168_G	171_H	0.565	0.98
109_S	123_V	0.564	0.98
171_H	174_L	0.560	0.98
130_V	146_G	0.555	0.98
217_I	221_G	0.555	0.98
113_L	123_V	0.554	0.98
69_W	72_F	0.554	0.98
225_L	241_F	0.550	0.98
63_V	68_V	0.549	0.98
182_I	190_Y	0.548	0.98
68_V	71_F	0.546	0.98
36_L	77_I	0.544	0.98
141_V	228_A	0.544	0.98
146_G	150_G	0.543	0.98
61_D	64_R	0.543	0.98
59_W	63_V	0.543	0.98
212_I	215_R	0.541	0.98
90_G	93_F	0.541	0.98
60_A	64_R	0.540	0.98
210_A	243_K	0.540	0.98
96_A	118_L	0.538	0.98
170_H	184_L	0.537	0.98
33_G	104_T	0.535	0.98
242_R	251_H	0.532	0.98
45_V	48_R	0.528	0.97
34_V	98_T	0.528	0.97
165_E	212_I	0.527	0.97
99_S	104_T	0.526	0.97
206_Y	234_Q	0.524	0.97
190_Y	193_G	0.522	0.97
37_G	46_V	0.522	0.97
173_S	191_K	0.521	0.97
143_D	148_T	0.521	0.97
183_S	186_E	0.520	0.97
112_V	115_S	0.519	0.97
114_E	120_V	0.512	0.97
209_M	237_V	0.512	0.97
35_D	147_G	0.511	0.97
40_D	192_R	0.510	0.97
208_K	212_I	0.510	0.97
36_L	40_D	0.508	0.97
206_Y	210_A	0.508	0.97
253_P	259_M	0.506	0.97
42_V	257_L	0.504	0.97
176_L	198_I	0.503	0.97
133_L	266_S	0.500	0.96
210_A	237_V	0.500	0.96

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
4jneA	2	0.8957	100	0.324	Contact Map
3d2fA	1	0.8921	100	0.324	Contact Map
4fl9A	1	0.8849	100	0.33	Contact Map
2v7yA	1	0.8993	100	0.331	Contact Map
3i33A	1	0.8777	100	0.361	Contact Map
4kboA	1	0.8705	100	0.365	Contact Map
4rtfD	1	0.8705	100	0.37	Contact Map
4j8fA	2	0.9137	100	0.371	Contact Map
4gniA	2	0.8741	100	0.398	Contact Map
3h1qA	1	0.946	100	0.399	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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