GREMLIN Database
NEMA - N-ethylmaleimide reductase
UniProt: P77258 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13546
Length: 365 (326)
Sequences: 11202 (7410)
Seq/√Len: 410.4

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
154_L203_Q3.6111.00
220_D264_R3.4461.00
99_A178_L3.0911.00
63_S163_D2.9351.00
207_S210_N2.5551.00
22_F320_A2.5151.00
169_A225_E2.5011.00
150_R156_E2.4881.00
165_R221_A2.4131.00
260_Q263_K2.3921.00
11_K16_T2.3801.00
91_V98_M2.3591.00
40_P86_K2.3331.00
152_L160_I2.3321.00
288_E317_L2.3291.00
10_L320_A2.2831.00
311_T315_K2.1721.00
52_A327_W2.1641.00
78_P170_N2.1461.00
232_G268_Y2.1271.00
152_L156_E2.1241.00
262_G295_H2.1221.00
92_H177_D2.1031.00
47_R94_E2.0611.00
14_A229_D2.0351.00
172_R226_W1.9871.00
299_I322_A1.9801.00
330_N336_R1.9741.00
7_Y338_Q1.9571.00
162_N166_Q1.9181.00
189_L215_V1.9121.00
335_A339_R1.8861.00
169_A173_E1.8811.00
259_E293_R1.8691.00
47_R90_G1.8561.00
310_E314_G1.8391.00
189_L214_L1.8351.00
60_T100_V1.8291.00
85_K89_A1.8241.00
166_Q170_N1.7971.00
159_G163_D1.7821.00
165_R225_E1.7631.00
154_L158_P1.7321.00
158_P217_E1.7271.00
111_A147_S1.7111.00
81_I173_E1.7101.00
5_K8_S1.6891.00
172_R227_G1.6831.00
215_V233_I1.6811.00
204_Y217_E1.6751.00
89_A93_A1.6621.00
256_Y260_Q1.6571.00
183_S233_I1.6521.00
210_N213_R1.6521.00
121_V152_L1.6501.00
5_K310_E1.6301.00
88_T176_F1.6051.00
154_L204_Y1.6031.00
189_L218_V1.5911.00
154_L157_I1.5881.00
153_E156_E1.5741.00
78_P173_E1.5641.00
288_E292_A1.5411.00
43_A86_K1.5321.00
162_N221_A1.5301.00
212_A260_Q1.5301.00
168_I222_G1.5291.00
65_Q159_G1.5221.00
195_P248_P1.4951.00
84_W176_F1.4771.00
40_P44_E1.4711.00
341_A344_N1.4691.00
158_P203_Q1.4681.00
22_F299_I1.4591.00
226_W230_R1.4531.00
291_R319_D1.4521.00
172_R230_R1.4431.00
215_V257_L1.4411.00
75_I100_V1.4411.00
48_Q332_D1.4241.00
44_E47_R1.4161.00
333_L337_L1.4131.00
38_P84_W1.3991.00
57_S98_M1.3921.00
212_A256_Y1.3881.00
26_L324_G1.3751.00
166_Q169_A1.3721.00
125_A151_A1.3701.00
81_I170_N1.3661.00
216_L260_Q1.3651.00
335_A338_Q1.3611.00
18_A97_H1.3581.00
32_I35_G1.3511.00
23_M49_R1.3501.00
5_K314_G1.3491.00
21_I334_V1.3451.00
75_I167_A1.3411.00
119_A150_R1.3271.00
216_L264_R1.3201.00
289_K293_R1.3181.00
309_A323_F1.3161.00
102_L167_A1.3141.00
250_E253_D1.3111.00
165_R169_A1.3091.00
208_V212_A1.3011.00
43_A90_G1.2951.00
219_V261_L1.2881.00
304_Y312_L1.2821.00
306_V337_L1.2811.00
161_V221_A1.2571.00
221_A224_E1.2511.00
10_L297_P1.2501.00
183_S189_L1.2441.00
17_A54_L1.2351.00
165_R224_E1.2301.00
306_V340_K1.2291.00
126_L199_H1.2231.00
212_A216_L1.2131.00
301_A322_A1.2121.00
158_P204_Y1.2091.00
151_A201_T1.2071.00
260_Q264_R1.2051.00
221_A225_E1.2051.00
23_M55_I1.2021.00
156_E159_G1.1881.00
83_A86_K1.1801.00
47_R93_A1.1751.00
327_W334_V1.1751.00
262_G296_G1.1741.00
15_I97_H1.1701.00
43_A47_R1.1671.00
289_K292_A1.1661.00
216_L220_D1.1651.00
185_H272_S1.1601.00
162_N165_R1.1561.00
37_I79_E1.1501.00
100_V171_A1.1461.00
312_L318_I1.1361.00
213_R217_E1.1341.00
126_L149_P1.1281.00
32_I39_T1.1271.00
251_E289_K1.1221.00
300_G318_I1.1201.00
190_H194_S1.1201.00
97_H177_D1.1181.00
68_G105_T1.1171.00
89_A92_H1.1131.00
57_S176_F1.1111.00
161_V217_E1.1101.00
226_W231_I1.1011.00
208_V256_Y1.0941.00
92_H98_M1.0931.00
88_T98_M1.0831.00
28_R41_L1.0831.00
284_D312_L1.0761.00
299_I320_A1.0761.00
183_S215_V1.0731.00
255_L293_R1.0671.00
81_I174_A1.0641.00
36_D67_K1.0631.00
203_Q213_R1.0571.00
161_V165_R1.0521.00
78_P82_A1.0511.00
233_I266_I1.0501.00
32_I37_I1.0501.00
306_V310_E1.0401.00
220_D224_E1.0361.00
256_Y259_E1.0321.00
39_T42_M1.0301.00
86_K89_A1.0211.00
11_K14_A1.0191.00
38_P83_A1.0151.00
163_D166_Q1.0131.00
251_E255_L1.0091.00
161_V218_V1.0051.00
17_A53_G1.0021.00
61_Q72_A0.9991.00
51_S335_A0.9991.00
122_A125_A0.9991.00
166_Q225_E0.9971.00
73_P84_W0.9961.00
23_M327_W0.9941.00
157_I161_V0.9901.00
17_A320_A0.9891.00
43_A87_I0.9881.00
107_R197_S0.9861.00
219_V266_I0.9861.00
5_K313_I0.9771.00
235_V254_A0.9741.00
17_A22_F0.9731.00
88_T174_A0.9701.00
158_P161_V0.9671.00
318_I321_V0.9601.00
77_S80_Q0.9581.00
121_V150_R0.9521.00
212_A257_L0.9501.00
304_Y321_V0.9481.00
180_E270_H0.9451.00
52_A334_V0.9431.00
233_I261_L0.9411.00
255_L289_K0.9381.00
74_G80_Q0.9371.00
178_L232_G0.9361.00
196_S247_G0.9281.00
19_N53_G0.9261.00
193_L215_V0.9251.00
155_E158_P0.9251.00
127_S148_M0.9231.00
258_I290_V0.9231.00
85_K173_E0.9221.00
255_L259_E0.9201.00
204_Y220_D0.9141.00
98_M177_D0.9111.00
75_I171_A0.9061.00
181_L222_G0.9031.00
54_L99_A0.9001.00
21_I333_L0.9001.00
188_L214_L0.8991.00
24_A270_H0.8971.00
258_I269_L0.8951.00
4_E310_E0.8921.00
13_G267_A0.8891.00
6_L309_A0.8861.00
18_A96_G0.8851.00
181_L218_V0.8831.00
37_I80_Q0.8811.00
212_A215_V0.8801.00
38_P73_P0.8791.00
98_M176_F0.8771.00
222_G231_I0.8771.00
268_Y299_I0.8721.00
151_A199_H0.8701.00
158_P221_A0.8671.00
208_V260_Q0.8671.00
82_A85_K0.8651.00
219_V264_R0.8541.00
307_E340_K0.8521.00
161_V214_L0.8511.00
15_I99_A0.8481.00
123_P197_S0.8461.00
223_I267_A0.8461.00
217_E220_D0.8461.00
59_A103_W0.8441.00
107_R149_P0.8441.00
161_V204_Y0.8431.00
10_L13_G0.8421.00
269_L296_G0.8421.00
208_V253_D0.8411.00
168_I226_W0.8371.00
54_L178_L0.8341.00
234_R270_H0.8311.00
23_M26_L0.8291.00
99_A232_G0.8271.00
24_A322_A0.8241.00
310_E313_I0.8211.00
56_I99_A0.8161.00
153_E158_P0.8151.00
258_I294_F0.8141.00
150_R153_E0.8141.00
209_E256_Y0.8111.00
206_G211_R0.8111.00
181_L219_V0.8091.00
123_P126_L0.8071.00
323_F326_D0.8041.00
271_M298_I0.8031.00
57_S84_W0.8031.00
249_N257_L0.8031.00
90_G94_E0.8021.00
332_D336_R0.8011.00
40_P43_A0.8011.00
105_T109_S0.8011.00
24_A324_G0.8001.00
14_A230_R0.8001.00
61_Q76_H0.7991.00
304_Y309_A0.7991.00
154_L161_V0.7981.00
38_P87_I0.7981.00
259_E263_K0.7961.00
281_P308_K0.7941.00
237_P271_M0.7841.00
307_E311_T0.7841.00
23_M52_A0.7831.00
77_S81_I0.7821.00
271_M290_V0.7811.00
160_I163_D0.7771.00
18_A51_S0.7761.00
203_Q210_N0.7671.00
61_Q67_K0.7641.00
44_E48_Q0.7611.00
214_L218_V0.7601.00
15_I178_L0.7601.00
165_R218_V0.7591.00
122_A126_L0.7571.00
56_I178_L0.7531.00
57_S100_V0.7491.00
190_H254_A0.7491.00
170_N173_E0.7481.00
273_E283_T0.7471.00
172_R225_E0.7451.00
63_S160_I0.7451.00
172_R229_D0.7451.00
4_E8_S0.7431.00
62_I164_F0.7411.00
223_I231_I0.7401.00
31_S42_M0.7401.00
323_F327_W0.7391.00
157_I215_V0.7381.00
323_F333_L0.7271.00
196_S241_F0.7231.00
182_H187_Y0.7221.00
152_L157_I0.7201.00
76_H80_Q0.7201.00
249_N252_A0.7181.00
45_Y49_R0.7171.00
125_A148_M0.7161.00
37_I77_S0.7121.00
232_G299_I0.7111.00
124_S199_H0.7101.00
108_I128_A0.7101.00
223_I227_G0.7071.00
90_G93_A0.7051.00
253_D256_Y0.7041.00
46_Y55_I0.7031.00
16_T97_H0.7011.00
21_I52_A0.7011.00
50_A94_E0.6991.00
52_A55_I0.6981.00
85_K88_T0.6981.00
309_A337_L0.6951.00
252_A255_L0.6941.00
169_A172_R0.6921.00
62_I218_V0.6911.00
249_N254_A0.6881.00
290_V294_F0.6881.00
60_T75_I0.6871.00
82_A86_K0.6841.00
92_H97_H0.6841.00
206_G210_N0.6841.00
305_T308_K0.6821.00
284_D300_G0.6801.00
37_I83_A0.6771.00
270_H322_A0.6761.00
50_A91_V0.6750.99
108_I242_Q0.6740.99
311_T314_G0.6730.99
209_E260_Q0.6710.99
112_S144_V0.6700.99
249_N253_D0.6700.99
126_L130_T0.6690.99
22_F56_I0.6660.99
22_F54_L0.6630.99
182_H185_H0.6570.99
219_V233_I0.6570.99
285_A288_E0.6550.99
62_I103_W0.6550.99
100_V176_F0.6550.99
312_L317_L0.6540.99
91_V96_G0.6510.99
25_P234_R0.6510.99
64_A76_H0.6510.99
63_S159_G0.6510.99
262_G294_F0.6490.99
66_A104_H0.6480.99
193_L212_A0.6480.99
12_V297_P0.6470.99
233_I269_L0.6460.99
290_V298_I0.6460.99
17_A97_H0.6420.99
241_F247_G0.6390.99
49_R332_D0.6390.99
203_Q217_E0.6370.99
31_S73_P0.6360.99
130_T146_T0.6340.99
326_D333_L0.6330.99
62_I163_D0.6310.99
75_I170_N0.6260.99
268_Y320_A0.6250.99
18_A338_Q0.6230.99
165_R222_G0.6230.99
190_H239_G0.6220.99
119_A147_S0.6200.99
107_R191_Q0.6200.99
7_Y334_V0.6180.99
193_L257_L0.6170.99
335_A341_A0.6160.99
300_G321_V0.6160.99
219_V231_I0.6150.99
22_F322_A0.6130.99
189_L222_G0.6110.99
65_Q160_I0.6100.99
208_V249_N0.6080.99
329_A344_N0.6080.99
9_P16_T0.6080.99
308_K311_T0.6070.99
86_K90_G0.6060.99
258_I293_R0.6030.99
261_L266_I0.6030.99
270_H301_A0.6020.99
24_A56_I0.5990.99
242_Q247_G0.5960.99
154_L217_E0.5960.99
54_L97_H0.5950.99
121_V156_E0.5930.99
75_I80_Q0.5930.99
81_I85_K0.5900.99
323_F337_L0.5880.99
270_H299_I0.5870.99
81_I88_T0.5870.99
72_A105_T0.5870.99
18_A95_N0.5870.99
218_V221_A0.5850.99
12_V17_A0.5840.99
311_T317_L0.5830.99
237_P286_F0.5830.99
227_G230_R0.5780.98
223_I228_A0.5770.98
164_F189_L0.5770.98
313_I321_V0.5730.98
152_L188_L0.5730.98
254_A290_V0.5710.98
308_K312_L0.5690.98
158_P162_N0.5690.98
65_Q114_Q0.5660.98
157_I189_L0.5660.98
239_G276_W0.5650.98
167_A170_N0.5640.98
73_P87_I0.5640.98
223_I226_W0.5630.98
326_D329_A0.5620.98
165_R226_W0.5610.98
82_A89_A0.5590.98
51_S338_Q0.5590.98
45_Y58_E0.5570.98
257_L260_Q0.5550.98
160_I188_L0.5540.98
99_A267_A0.5540.98
208_V257_L0.5530.98
25_P270_H0.5490.98
27_T68_G0.5490.98
126_L129_G0.5460.98
180_E232_G0.5440.98
26_L328_I0.5440.98
88_T100_V0.5430.98
216_L256_Y0.5420.98
70_A113_L0.5410.98
65_Q163_D0.5410.98
160_I164_F0.5400.98
232_G270_H0.5390.98
332_D335_A0.5380.98
113_L116_G0.5370.98
66_A105_T0.5370.98
21_I327_W0.5360.97
294_F297_P0.5350.97
42_M58_E0.5350.97
23_M324_G0.5340.97
205_G211_R0.5340.97
104_H160_I0.5340.97
222_G225_E0.5330.97
235_V271_M0.5310.97
179_V226_W0.5290.97
181_L231_I0.5270.97
154_L202_D0.5270.97
222_G226_W0.5250.97
313_I318_I0.5200.97
122_A197_S0.5190.97
61_Q105_T0.5190.97
306_V333_L0.5190.97
291_R298_I0.5180.97
7_Y337_L0.5170.97
157_I204_Y0.5160.97
309_A321_V0.5150.97
125_A199_H0.5140.97
57_S60_T0.5140.97
29_L69_Y0.5130.97
223_I264_R0.5130.97
72_A103_W0.5120.97
194_S239_G0.5110.97
114_Q120_P0.5110.97
55_I96_G0.5100.97
181_L189_L0.5090.97
13_G223_I0.5080.97
38_P42_M0.5080.97
68_G103_W0.5070.96
24_A327_W0.5050.96
252_A256_Y0.5020.96
46_Y58_E0.5020.96
336_R341_A0.5020.96
236_S239_G0.5010.96
121_V160_I0.5000.96
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4e2bA 1 0.9808 100 0.21 Contact Map
2hsaB 2 0.9781 100 0.211 Contact Map
4qnwA 1 0.9479 100 0.219 Contact Map
3tjlA 2 0.9808 100 0.226 Contact Map
4tmbA 2 0.9781 100 0.227 Contact Map
4jicA 1 0.9753 100 0.229 Contact Map
4gbuA 2 0.9781 100 0.238 Contact Map
4awtA 1 0.9836 100 0.241 Contact Map
4a3uA 1 0.9671 100 0.245 Contact Map
1vyrA 1 0.989 100 0.25 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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