GREMLIN Database
PRPD - 2-methylcitrate dehydratase
UniProt: P77243 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_01
EcoGene: EG13603
Length: 483 (432)
Sequences: 2710 (1861)
Seq/√Len: 89.6

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
184_A194_I5.4371.00
190_T193_E4.2411.00
75_R125_D3.9281.00
318_E412_T3.8881.00
319_K384_N3.8461.00
21_D191_R3.7071.00
38_H467_A3.2581.00
426_I438_K3.1741.00
408_T418_E3.0031.00
321_T386_F2.9381.00
46_G443_F2.8211.00
300_A352_V2.8071.00
318_E410_E2.7091.00
411_F417_F2.6471.00
147_A186_M2.6021.00
323_R388_D2.5941.00
306_Q419_E2.5601.00
321_T408_T2.5531.00
39_Y435_G2.5411.00
378_A382_K2.5311.00
316_D412_T2.4911.00
301_M355_P2.4901.00
197_A239_A2.4271.00
358_F365_D2.4271.00
319_K410_E2.4231.00
323_R406_A2.4201.00
42_L460_L2.3641.00
321_T384_N2.3601.00
155_Q178_A2.3311.00
37_A149_I2.3271.00
232_A236_A2.3221.00
35_D435_G2.3171.00
410_E416_R2.2501.00
187_L237_L2.2491.00
29_S149_I2.2081.00
33_A279_E2.2041.00
289_A293_S2.2011.00
123_T183_V2.0971.00
41_L148_M2.0721.00
444_K457_Q2.0711.00
465_D468_R2.0551.00
321_T410_E2.0451.00
164_F173_L2.0341.00
440_V464_L2.0151.00
123_T233_V2.0111.00
44_T96_I1.9901.00
30_S33_A1.9821.00
120_I182_V1.9771.00
434_D438_K1.9711.00
146_T185_E1.9551.00
37_A145_L1.9521.00
313_T316_D1.9451.00
120_I144_V1.9361.00
62_G241_T1.8871.00
323_R408_T1.8371.00
360_R365_D1.8151.00
181_A198_V1.8141.00
26_Y142_K1.8031.00
449_R452_P1.7881.00
25_N28_I1.7801.00
26_Y34_Y1.7791.00
322_I352_V1.7711.00
200_L220_R1.7641.00
180_T197_A1.7511.00
24_M191_R1.7461.00
210_T253_P1.7421.00
32_V279_E1.7311.00
444_K456_Q1.7291.00
96_I115_D1.7141.00
358_F373_D1.7131.00
457_Q461_E1.6921.00
117_L148_M1.6811.00
123_T187_L1.6561.00
443_F447_L1.6441.00
323_R386_F1.6411.00
411_F415_T1.6281.00
22_Y276_Y1.6031.00
49_L447_L1.5981.00
297_V355_P1.5941.00
42_L463_S1.5931.00
104_T282_L1.5761.00
302_T419_E1.5741.00
202_W250_L1.5641.00
27_E142_K1.5601.00
350_Y380_R1.5331.00
408_T416_R1.5311.00
312_K411_F1.5231.00
143_Q185_E1.5211.00
34_Y38_H1.5151.00
272_P275_S1.5111.00
307_M312_K1.5081.00
158_I177_V1.5011.00
85_P89_A1.4931.00
187_L236_A1.4931.00
440_V460_L1.4861.00
444_K460_L1.4841.00
57_C95_M1.4821.00
406_A418_E1.4821.00
151_A182_V1.4701.00
74_V88_A1.4580.99
357_L376_I1.4530.99
299_A422_V1.4530.99
222_S345_D1.4470.99
143_Q146_T1.4410.99
85_P126_W1.4300.99
29_S34_Y1.4290.99
461_E465_D1.4250.99
67_G241_T1.4210.99
304_Y359_G1.4140.99
354_I365_D1.4120.99
187_L240_K1.4040.99
34_Y146_T1.4020.99
358_F370_V1.4000.99
123_T127_L1.3980.99
180_T232_A1.3970.99
76_V83_L1.3860.99
41_L145_L1.3850.99
123_T186_M1.3840.99
48_G96_I1.3820.99
462_V469_L1.3800.99
177_V198_V1.3750.99
452_P456_Q1.3620.99
378_A381_E1.3440.99
440_V444_K1.3430.99
377_D380_R1.3320.99
230_S234_R1.3290.99
22_Y273_Y1.3290.99
290_E346_H1.3170.99
30_S276_Y1.3110.99
299_A407_I1.3080.99
65_V91_N1.3030.99
39_Y439_L1.3030.99
200_L239_A1.2900.99
75_R82_Q1.2880.99
334_K337_P1.2880.99
124_A144_V1.2810.99
320_V409_L1.2800.99
342_A345_D1.2780.99
23_V194_I1.2760.99
154_I181_A1.2720.99
453_T457_Q1.2710.99
20_V24_M1.2640.98
28_I146_T1.2620.98
184_A189_L1.2610.98
16_I195_L1.2590.98
58_K62_G1.2560.98
23_V181_A1.2530.98
357_L379_L1.2530.98
227_D230_S1.2500.98
45_L463_S1.2490.98
298_E426_I1.2470.98
34_Y145_L1.2450.98
120_I183_V1.2440.98
88_A92_I1.2410.98
104_T291_F1.2350.98
24_M194_I1.2310.98
297_V351_M1.2250.98
20_V195_L1.2220.98
143_Q188_G1.2190.98
120_I148_M1.2150.98
293_S351_M1.2120.98
315_A412_T1.2020.98
170_D224_A1.2020.98
26_Y29_S1.1990.98
424_Y430_R1.1960.98
362_T365_D1.1950.98
40_C283_F1.1880.98
420_V423_E1.1810.98
339_N343_D1.1800.98
189_L239_A1.1730.98
105_W109_E1.1650.97
59_K219_T1.1610.97
144_V148_M1.1600.97
105_W277_V1.1570.97
72_N82_Q1.1560.97
471_Q475_N1.1550.97
289_A366_Y1.1540.97
36_T39_Y1.1500.97
351_M366_Y1.1470.97
437_P440_V1.1390.97
315_A375_R1.1300.97
300_A409_L1.1290.97
48_G77_P1.1280.97
146_T150_K1.1240.97
303_L409_L1.1120.97
114_S152_H1.1060.96
70_V73_G1.1040.96
238_M243_E1.1030.96
203_V224_A1.1000.96
51_A95_M1.0930.96
74_V125_D1.0910.96
333_D349_Q1.0880.96
98_W222_S1.0850.96
333_D337_P1.0750.96
189_L193_E1.0750.96
77_P121_L1.0720.96
183_V236_A1.0720.96
227_D231_R1.0680.96
119_G179_S1.0670.96
114_S117_L1.0670.96
90_F226_G1.0660.96
97_R102_N1.0590.95
69_V72_N1.0570.95
196_N244_M1.0570.95
46_G446_N1.0560.95
84_D87_Q1.0520.95
447_L456_Q1.0490.95
203_V220_R1.0470.95
387_E392_T1.0470.95
189_L197_A1.0450.95
335_K349_Q1.0410.95
287_F298_E1.0320.95
59_K243_E1.0310.95
119_G183_V1.0310.95
181_A194_I1.0260.95
17_V191_R1.0190.94
17_V192_E1.0170.94
286_S291_F1.0160.94
93_G224_A1.0120.94
326_E392_T1.0100.94
307_M311_G1.0090.94
459_I463_S1.0070.94
100_D221_K1.0060.94
42_L440_V1.0040.94
57_C91_N0.9990.94
83_L91_N0.9930.94
282_L291_F0.9900.94
202_W208_L0.9880.93
277_V280_N0.9870.93
193_E196_N0.9870.93
317_I409_L0.9830.93
183_V232_A0.9760.93
208_L260_V0.9720.93
283_F439_L0.9680.93
14_R18_D0.9640.93
189_L240_K0.9580.92
90_F93_G0.9550.92
76_V91_N0.9460.92
189_L236_A0.9450.92
77_P92_I0.9430.92
434_D437_P0.9380.92
348_I385_C0.9380.92
177_V202_W0.9370.91
35_D39_Y0.9350.91
297_V361_L0.9340.91
297_V300_A0.9290.91
441_D445_I0.9240.91
122_A125_D0.9220.91
176_K232_A0.9210.91
17_V195_L0.9160.91
291_F294_Q0.9160.91
60_L94_A0.9160.91
185_E194_I0.9100.90
219_T243_E0.9070.90
89_A122_A0.9040.90
26_Y145_L0.9010.90
128_S471_Q0.9000.90
427_G432_R0.8980.90
180_T236_A0.8940.89
34_Y149_I0.8920.89
460_L463_S0.8890.89
300_A356_L0.8880.89
317_I356_L0.8800.89
288_P361_L0.8760.89
196_N239_A0.8760.89
179_S232_A0.8750.88
288_P365_D0.8730.88
94_A226_G0.8730.88
452_P455_Q0.8710.88
172_V207_S0.8700.88
87_Q91_N0.8690.88
43_D442_K0.8670.88
34_Y142_K0.8640.88
390_A393_A0.8590.88
127_L240_K0.8540.87
470_E476_E0.8540.87
221_K224_A0.8540.87
126_W237_L0.8510.87
336_G381_E0.8510.87
406_A420_V0.8490.87
85_P122_A0.8490.87
353_A379_L0.8480.87
97_R224_A0.8480.87
106_L291_F0.8460.87
311_G359_G0.8400.86
181_A184_A0.8360.86
97_R345_D0.8300.86
74_V92_I0.8280.86
89_A119_G0.8250.85
17_V21_D0.8230.85
23_V185_E0.8220.85
157_C272_P0.8200.85
311_G314_A0.8180.85
316_D413_D0.8150.85
237_L241_T0.8130.84
22_Y153_E0.8100.84
23_V184_A0.8100.84
51_A98_W0.8090.84
455_Q458_R0.8090.84
322_I348_I0.8060.84
191_R195_L0.8050.84
356_L379_L0.8050.84
304_Y355_P0.8040.84
93_G226_G0.8010.84
109_E159_A0.7990.83
219_T244_M0.7970.83
292_H395_Y0.7960.83
350_Y354_I0.7940.83
322_I383_I0.7920.83
193_E240_K0.7890.83
196_N245_G0.7880.83
100_D346_H0.7870.83
375_R378_A0.7870.83
102_N221_K0.7860.82
292_H325_H0.7850.82
318_E382_K0.7830.82
296_A348_I0.7790.82
150_K185_E0.7780.82
66_P81_F0.7770.82
299_A419_E0.7760.82
296_A352_V0.7760.82
461_E464_L0.7710.81
41_L117_L0.7690.81
447_L451_F0.7680.81
286_S433_Q0.7670.81
104_T403_I0.7640.81
304_Y356_L0.7630.81
86_V237_L0.7630.81
451_F456_Q0.7600.80
300_A304_Y0.7600.80
220_R224_A0.7580.80
59_K62_G0.7580.80
296_A322_I0.7580.80
97_R217_T0.7570.80
388_D391_F0.7570.80
203_V208_L0.7510.79
307_M417_F0.7510.79
92_I125_D0.7480.79
456_Q459_I0.7480.79
210_T249_A0.7470.79
44_T117_L0.7470.79
176_K205_G0.7460.79
157_C270_Q0.7460.79
97_R170_D0.7450.79
15_E251_T0.7450.79
167_V263_K0.7420.79
61_L79_T0.7380.78
358_F376_I0.7380.78
164_F174_L0.7370.78
97_R175_V0.7370.78
172_V221_K0.7360.78
231_R235_L0.7360.78
119_G182_V0.7360.78
458_R462_V0.7330.78
353_A383_I0.7320.78
23_V198_V0.7310.78
354_I376_I0.7300.78
326_E330_R0.7300.78
358_F375_R0.7290.77
314_A357_L0.7290.77
189_L194_I0.7260.77
312_K316_D0.7250.77
116_N151_A0.7250.77
92_I122_A0.7220.77
103_D114_S0.7210.77
17_V248_S0.7200.77
466_R470_E0.7180.76
83_L87_Q0.7170.76
444_K448_A0.7170.76
288_P446_N0.7170.76
354_I358_F0.7140.76
454_R458_R0.7130.76
254_V259_D0.7090.75
206_Q261_S0.7080.75
35_D464_L0.7080.75
37_A278_M0.7080.75
50_E99_L0.7080.75
300_A307_M0.7060.75
142_K470_E0.7050.75
322_I407_I0.7040.75
304_Y307_M0.7030.75
99_L290_E0.7030.75
165_N172_V0.7030.75
37_A148_M0.7030.75
245_G345_D0.7020.75
170_D203_V0.6980.74
26_Y146_T0.6980.74
324_T385_C0.6980.74
16_I199_S0.6970.74
128_S475_N0.6970.74
101_F288_P0.6950.74
105_W159_A0.6950.74
212_R346_H0.6930.74
61_L91_N0.6910.74
170_D221_K0.6900.73
97_R115_D0.6880.73
435_G464_L0.6860.73
31_K35_D0.6850.73
105_W204_D0.6840.73
447_L450_Q0.6830.73
160_L277_V0.6800.72
175_V207_S0.6800.72
50_E363_A0.6790.72
35_D38_H0.6790.72
41_L144_V0.6780.72
334_K343_D0.6780.72
320_V379_L0.6740.72
325_H328_C0.6730.72
97_R225_A0.6730.72
46_G49_L0.6720.72
65_V68_T0.6710.71
297_V301_M0.6690.71
16_I251_T0.6690.71
344_R371_A0.6690.71
90_F230_S0.6690.71
73_G85_P0.6690.71
382_K412_T0.6690.71
86_V230_S0.6670.71
360_R441_D0.6640.71
101_F446_N0.6620.70
314_A411_F0.6620.70
352_V356_L0.6620.70
124_A127_L0.6610.70
218_G222_S0.6590.70
165_N212_R0.6580.70
221_K345_D0.6580.70
195_L248_S0.6550.70
355_P361_L0.6520.69
357_L375_R0.6520.69
47_C101_F0.6510.69
217_T347_C0.6510.69
370_V373_D0.6510.69
426_I431_R0.6500.69
175_V228_A0.6490.69
181_A197_A0.6480.69
44_T48_G0.6480.69
104_T111_G0.6470.69
120_I177_V0.6460.69
270_Q273_Y0.6460.69
113_P156_G0.6460.69
36_T40_C0.6450.68
451_F455_Q0.6420.68
212_R222_S0.6420.68
300_A355_P0.6420.68
296_A405_N0.6410.68
199_S203_V0.6410.68
444_K453_T0.6410.68
127_L186_M0.6400.68
32_V35_D0.6400.68
438_K441_D0.6380.68
172_V175_V0.6370.67
73_G126_W0.6330.67
291_F428_H0.6330.67
237_L240_K0.6320.67
209_R212_R0.6310.67
74_V85_P0.6290.66
149_I278_M0.6290.66
361_L366_Y0.6280.66
116_N120_I0.6270.66
456_Q460_L0.6270.66
361_L365_D0.6260.66
291_F425_P0.6260.66
341_P371_A0.6250.66
153_E276_Y0.6240.66
20_V194_I0.6240.66
307_M409_L0.6230.66
20_V191_R0.6220.66
342_A346_H0.6220.66
304_Y314_A0.6210.66
287_F426_I0.6210.66
329_I385_C0.6200.65
426_I434_D0.6200.65
103_D221_K0.6190.65
216_N248_S0.6190.65
389_P393_A0.6190.65
208_L250_L0.6170.65
165_N173_L0.6150.65
29_S146_T0.6140.65
292_H328_C0.6140.65
318_E321_T0.6120.64
394_D402_A0.6120.64
292_H331_I0.6110.64
56_A98_W0.6080.64
97_R207_S0.6080.64
164_F170_D0.6080.64
171_H209_R0.6070.64
169_L261_S0.6050.63
307_M411_F0.6040.63
273_Y276_Y0.6040.63
39_Y464_L0.6040.63
168_G221_K0.6010.63
372_Q377_D0.6010.63
306_Q415_T0.6010.63
142_K457_Q0.5980.63
457_Q460_L0.5980.63
446_N449_R0.5970.62
354_I361_L0.5940.62
251_T258_Y0.5940.62
116_N182_V0.5930.62
42_L464_L0.5930.62
53_E58_K0.5920.62
106_L290_E0.5920.62
15_E19_I0.5920.62
69_V84_D0.5920.62
100_D112_H0.5910.62
374_K389_P0.5910.62
153_E157_C0.5860.61
247_P253_P0.5850.61
44_T118_G0.5840.61
97_R174_L0.5830.61
168_G172_V0.5830.61
470_E475_N0.5830.61
188_G191_R0.5810.60
326_E387_E0.5810.60
119_G233_V0.5800.60
54_Y57_C0.5800.60
102_N207_S0.5790.60
105_W156_G0.5770.60
178_A182_V0.5770.60
380_R383_I0.5760.60
123_T126_W0.5760.60
471_Q476_E0.5760.60
113_P281_V0.5760.60
465_D469_L0.5740.59
190_T194_I0.5730.59
462_V465_D0.5730.59
38_H463_S0.5730.59
96_I117_L0.5720.59
75_R92_I0.5720.59
172_V290_E0.5710.59
167_V262_F0.5710.59
350_Y370_V0.5710.59
288_P362_T0.5700.59
124_A474_V0.5700.59
349_Q380_R0.5690.59
120_I179_S0.5680.59
64_I68_T0.5670.59
267_F273_Y0.5670.59
171_H292_H0.5660.58
290_E332_I0.5660.58
91_N122_A0.5650.58
25_N146_T0.5650.58
226_G229_T0.5650.58
33_A36_T0.5640.58
28_I34_Y0.5630.58
306_Q417_F0.5630.58
422_V431_R0.5610.58
204_D235_L0.5580.57
469_L476_E0.5580.57
435_G440_V0.5580.57
40_C117_L0.5570.57
27_E30_S0.5560.57
120_I151_A0.5550.57
100_D104_T0.5550.57
114_S288_P0.5530.57
249_A254_V0.5530.57
177_V180_T0.5520.57
44_T283_F0.5520.57
295_T405_N0.5520.57
144_V474_V0.5520.57
160_L280_N0.5500.56
97_R222_S0.5490.56
267_F271_R0.5490.56
402_A424_Y0.5490.56
458_R476_E0.5490.56
21_D25_N0.5480.56
28_I185_E0.5470.56
97_R221_K0.5470.56
368_D371_A0.5460.56
20_V180_T0.5460.56
341_P368_D0.5450.56
44_T47_C0.5450.56
152_H446_N0.5440.55
26_Y149_I0.5440.55
100_D209_R0.5440.55
62_G66_P0.5430.55
356_L411_F0.5430.55
173_L206_Q0.5430.55
300_A407_I0.5420.55
105_W110_W0.5420.55
317_I320_V0.5410.55
47_C100_D0.5400.55
221_K442_K0.5400.55
207_S224_A0.5400.55
164_F172_V0.5400.55
245_G250_L0.5390.55
40_C278_M0.5380.55
211_Y222_S0.5370.54
298_E302_T0.5370.54
32_V429_A0.5370.54
40_C281_V0.5360.54
152_H278_M0.5360.54
360_R445_I0.5360.54
378_A383_I0.5360.54
324_T328_C0.5350.54
18_D21_D0.5350.54
290_E428_H0.5340.54
306_Q458_R0.5340.54
314_A417_F0.5330.54
38_H145_L0.5330.54
200_L235_L0.5330.54
23_V154_I0.5330.54
316_D415_T0.5310.54
412_T444_K0.5310.54
170_D207_S0.5310.54
97_R346_H0.5300.54
44_T114_S0.5300.54
152_H281_V0.5290.53
150_K181_A0.5290.53
159_A164_F0.5290.53
348_I352_V0.5290.53
39_Y434_D0.5280.53
57_C98_W0.5260.53
104_T292_H0.5250.53
30_S279_E0.5230.53
345_D366_Y0.5230.53
109_E171_H0.5230.53
76_V154_I0.5210.52
383_I407_I0.5210.52
217_T345_D0.5210.52
119_G123_T0.5200.52
102_N170_D0.5180.52
55_P58_K0.5170.52
312_K417_F0.5170.52
364_A449_R0.5150.51
100_D114_S0.5140.51
60_L91_N0.5130.51
334_K349_Q0.5130.51
176_K201_A0.5120.51
123_T237_L0.5100.51
30_S273_Y0.5100.51
290_E345_D0.5100.51
317_I411_F0.5090.51
390_A394_D0.5080.51
172_V223_W0.5080.51
158_I251_T0.5070.50
68_T86_V0.5070.50
105_W111_G0.5070.50
303_L307_M0.5050.50
320_V383_I0.5050.50
307_M316_D0.5050.50
458_R461_E0.5030.50
332_I346_H0.5030.50
315_A415_T0.5030.50
164_F171_H0.5020.50
52_L95_M0.5010.50
149_I416_R0.5000.49
153_E278_M0.5000.49
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1szqA 2 0.9752 100 0.127 Contact Map
2hp0A 2 0.9006 100 0.146 Contact Map
4rqoA 2 0.5321 96.3 0.946 Contact Map
1nm8A 1 0.1222 33.5 0.974 Contact Map
4giuA 2 0.0994 28.4 0.975 Contact Map
1xl7A 1 0.1284 18.2 0.977 Contact Map
1ji8A 1 0.1532 15.2 0.978 Contact Map
3kp1E 1 0.1118 13.6 0.978 Contact Map
1wx1A 2 0.5714 13.6 0.978 Contact Map
1t1uA 1 0.1201 9.1 0.98 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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